Optimization and cost reduction with performance improvements on web pages

Comparison between websites to improve their performance and optimization. Applied bibliographic research to understand and survey the concepts applied at work, adopting quantitative research through a questionnaire aimed at random audiences, documentary research to support important information on websites, informal magazines and descriptive research to analyze the effect of this study based on in the information presented. Five sites were adopted, one of which is a previous version and the other is a current one, with different characteristics to check its performance and optimization on the web. As explained, it was possible to verify the bottlenecks regarding the performance and optimization of the websites. With the applications of the necessary tools it was possible to improve the performance of the sites and with that the loading of the page became faster due to the compression in the loading of the images and also in the use of JavaScript, Css and Html. With the tools properly presented to improve performance and optimization, it is prominent to express the due improvements of the sites used in everyday life

VIVER COM HIV/AIDS EM SITUAÇÃO DE RUA: REPRESENTAÇÕES SOCIAIS DE PESSOAS HOSPITALIZADAS

Objetivo: apreender as representações sociais sobre o viver com HIV para pessoas hospitalizadas em situação de rua e identificar os conteúdos, elementos e estrutura dessas representações. Método: trata-se de estudo descritivo, embasado na Teoria das Representações Sociais, realizado com pessoas hospitalizadas, que vivem com HIV em situação de rua. Para coleta de dados utilizou-se um formulário e o Teste de Associação Livre de Palavras. A análise de dados ocorreu por meio da estatística descritiva e do software EVOC. Resultados: dos 65 participantes, 46 eram do sexo masculino, com idade média de 39 anos. Observou-se como núcleo central das representações sociais: medo, doente e preconceito, indicando as proporções funcionais e relacionadas à imagem do objeto investigado. O grupo investigado representou o viver com HIV/aids na rua por meio de palavras negativas, carregadas de mágoa, tristeza e medo. Conclusão: as representações têm um provável núcleo central na palavra “medo”. Descritores: Representação Social. Pessoas Mal Alojadas. HIV. Hospitalização. Enfermagem

ESTUDOS E IMPLEMENTAÇÃO DA METODOLOGIA TPM NO LABORATÓRIO DE PROCESSOS DE FABRICAÇÃO DA UniEVANGÉLICA

Este trabalho teve por objetivo realizar estudos sobre a metodologia da Manutenção Preditiva Total (TPM) e implantar o 1º pilar dos 8 pilares desse conjunto de técnicas e procedimentos no laboratório de Processos de Fabricação, do Centro Tecnológico, da UniEVANGÉLICA. A metodologia utilizada foi, inicialmente, o estudo das principais bibliografias sobre a filosofia TPM envolvendo os 8 pilares, e em segundo momento foi realizada a implantação do 1º pilar desse conjunto de técnicas. A partir de levantamentos foram realizadas intervenções técnicas e capacitações embasadas e estruturadas no 1º pilar da TPM: Manutenção Autônoma. Com a implementação da manutenção autônoma foi possível verificar as consequências das melhorias dessa ferramenta de gestão da manutenção como: melhor disposição de máquinas e equipamentos, higiene e segurança no trabalho, controle de realização das manutenções assim como, o planejamento das manutenções preventivas e preditivas, além de proporcionar um melhor ambiente de trabalho e propício para desenvolvimento do aprendizado.

Microbial community structure and dynamics in thermophilic composting viewed through metagenomics and metatranscriptomics

Composting is a promising source of new organisms and thermostable enzymes that may be helpful in environmental management and industrial processes. Here we present results of metagenomicand metatranscriptomic-based analyses of a large composting operation in the Sao Paulo Zoo Park. This composting exhibits a sustained thermophilic profile (50 degrees C to 75 degrees C), which seems to preclude fungal activity. The main novelty of our study is the combination of time-series sampling with shotgun DNA, 16S rRNA gene amplicon, and metatranscriptome high-throughput sequencing, enabling an unprecedented detailed view of microbial community structure, dynamics, and function in this ecosystem. The time-series data showed that the turning procedure has a strong impact on the compost microbiota, restoring to a certain extent the population profile seen at the beginning of the processand that lignocellulosic biomass deconstruction occurs synergistically and sequentially, with hemicellulose being degraded preferentially to cellulose and lignin. Moreover, our sequencing data allowed near-complete genome reconstruction of five bacterial species previously found in biomass-degrading environments and of a novel biodegrading bacterial species, likely a new genus in the order Bacillales. The data and analyses provided are a rich source for additional investigations of thermophilic composting microbiology.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Provost's Office for Research of the University of Sao PauloCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, BrazilUniv Sao Paulo, Programa Pos Graduacao Interunidades Bioinformat, Sao Paulo, BrazilUniv Sao Paulo, Escola Artes Ciencias & Humanidades, Sao Paulo, Brazil|Fundacao Parque Zool Sao Paulo, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Ciencias Biol, Sao Paulo, BrazilBiocomplex Inst Virginia, Blacksburg, VA USADepartamento de Ciências Biológicas, Universidade Federal de São Paulo, São Paulo, BrazilFAPESP: 2011/50870-6Web of Scienc

Antinociceptive and Anti-Inflammatory Activity from Algae of the Genus Caulerpa

Marine natural products have been the focus of discovery for new products of chemical and pharmacological interest. The aim of this study was to evaluate the antinociceptive activity of the methanolic (ME), acetate (AE), hexanic (HE) and chloroform (CE) extracts obtained from Caulerpa mexicana, and ME, CE and HE obtained from Caulerpa sertularioides. These marine algae are found all over the world, mainly in tropical regions. Models such as the writhing test, the hot plate test and formalin-induced nociception test were used to evaluate antinociceptive activity in laboratory mice. In the writhing test, all the extracts were administered orally at a concentration of 100 mg/kg, and induced high peripheral antinociceptive activity, with a reduction in the nociception induced by acetic acid above 65%. In the hot plate test, treatment with extracts from C. sertularioides (100 mg/kg, p.o.) did not significantly increase the latency of response, although the ME, AE and HE from C. mexicana showed activity in this model. This result suggests that these extracts exhibit antinociceptive activity. In the formalin test, it was observed that ME, AE and HE obtained from C. mexicana reduced the effects of formalin in both phases. On the other hand only CE from C. sertularioides induced significant inhibition of the nociceptive response in the first phase. To better assess the potential anti-inflammatory activity of the extracts, the carrageenan-induced peritonitis test was used to test Caulerpa spp. extracts on cell migration into the peritoneal cavity. In this assay, all extracts evaluated were able to significantly inhibit leukocyte migration into the peritoneal cavity in comparison with carrageenan. These data demonstrate that extracts from Caulerpa species elicit pronounced antinociceptive and anti-inflamatory activity against several nociception models. However, pharmacological and chemical studies are continuing in order to characterize the mechanism(s) responsible for the antinociceptive action and also to identify the active principles present in the Caulerpa species

Uso de substratos alternativos no sistema hidropônico e a influência na produção de mudas de Lactuca sativa L. / Use alternative substrates in the hydroponic system influence on production Lactuca sativa L. seedlings

O Estado do Piauí possui alguns entraves na produção da Lactuca sativa L. devido principalmente as condições edafoclimáticas, por isso, veio a necessidade de adoções de novas tecnologias, utilizando o cultivo hidropônico com substratos alternativos, que vem apresentando diversas vantagens, dentre elas, o manejo adequado da água, o melhor desempenho fisiológico e desenvolvimento das plântulas, além da sustentabilidade e economia na produção. Portanto, o objetivo do presente trabalho foi avaliar o uso dos substratos alternativos no sistema hidropônico em função do desempenho agronômico em mudas de alface (L. sativa). O experimento foi conduzido no período entre 16 de junho a 11 de julho de 2016, em casa de vegetação localizada no Município de Uruçuí – PI, a hortaliça utilizada foi a alface, cultivar Crespa Caipira. Os materiais para compor os tratamentos foram cedidos pelo produtor de hortaliças (espuma fenólica) e os substratos alternativos foram obtidos de propriedade de pequeno produtor rural (folha de piaçava, pecíolo e folha de buriti). O delineamento experimental utilizado foi o inteiramente casualizado (DIC), com sete tratamentos em quatro repetições. Os tratamentos utilizados foram: T1 – Espuma Fenólica, T2 – Folha de Piaçava, T3 – Folha de Buriti, T4 – Pecíolo de Buriti, T5 – Folha de Piaçava 50% + Pecíolo de Buriti 50%, T6 – Folha de Piaçava 50% + Folha de Buriti 50% e T7 – Folha de Piaçava 33% + Folha de Buriti 33% + Pecíolo de Buriti 33%. Avaliaram-se a porcentagem de germinação (PG), o número de folhas (NF), o diâmetro de caule (DC), o comprimento da parte aérea (CPA), o comprimento radicular (CR), a matéria fresca da parte aérea (MFPA) e a matéria seca da parte aérea (MSPA). Os dados foram submetidos à análise de variância pelo teste F, e quando significativos, foi utilizado o teste de Tukey (P ? 0,05) para comparação das médias no programa ASSISTAT 7.7. trabalhos com o uso de folhas e pecíolos de buriti em cultivos hidropônicos, tem se destacando devido ao pioneirismo deste trabalho e dando relevância como mais uma alternativa de renda e ambientalmente sustentável, para os produtores rurais. A utilização da Folha e do Pecíolo de Mauritia flexuosa como substratos no sistema hidropônico, proporcionaram bom desempenho agronômico em mudas de Lactuca sativa.

Combinations of Metarhizium anisopliae with chemical insecticides and their effectiveness in Mahanarva fimbriolata (Hemiptera: Cercopidae) control on sugarcane

Some insecticides can be used jointly with entomopathogenic fungi, and therefore the combi- nation of chemical and biological control measures can be a safe and effective method to con- trol insect pests. The aim of this study was to evaluate the costs and efficacy of combinations of Metarhizium anisopliae (Metschnikoff) Sorokin (Hypocreales: Clavicipitaceae) with thiameth- oxam and imidacloprid on spittlebug (Mahanarva fimbriolata (Stål); Hemiptera: Cercopidae) control on sugarcane. The experiment was conducted as a randomized block design (RBD) with 10 treatments and 4 replications. The treatments included a control (untreated), thia- −1 −1 12 −1 methoxam (250 g ha ), imidacloprid (700 g ha ), M. anisopliae (M. a.) (3 × 10 conidia ha ), A1 (3 × 10 12 M. a. conidia ha −1 + 65 g ha −1 of thiamethoxam), A2 (3 × 10 12 M. a. conidia ha −1 + 125 g ha −1 of thiamethoxam), A3 (3 × 10 12 M. a. conidia ha −1 + 187.5 g ha −1 of thiamethoxam), A4 (3 × 10 12 M. a. conidia ha −1 + 175 g ha −1 of imidacloprid), A5 (3 × 10 12 M. a. conidia ha −1 + 350 g ha −1 of imidacloprid), and A6 (3 × 10 12 M. a. conidia ha −1 + 525g ha −1 of imidacloprid). The reductions in the numbers of M. fimbriolata nymphs per treatment compared to the control were similar at 15 DAT (days after treatment) in all treatments except combination A5 (M. anisopliae and thiamethoxam). At 30 DAT, the numbers of nymphs were significantly reduced in all treatments except A3, and their effectiveness ranged from 14.28% to 92.85%. At 45 DAT the numbers of M. fimbriolata nymphs per treatment were significantly reduced in the following treatments: imidacloprid alone at 700g ha -1 , A1, A2, A3, A4 and A6; and the combinations A1 and A2 caused the lowest M. fimbriolata nymph infestations and effectiveness rates of 77.41 and 87.09 %, respectively. At 75 DAT the 2 best control efficacies occurred in treatments A1 (3 × 10 12 M. a. conidia ha -1 of + 65g ha -1 of thiamethoxam) (82.1%) and A5 (78.6%) (3 × 10 12 M. a. conidia ha −1 + 350 g ha −1 of imidacloprid). At 90 DAT the number of nymphs in the control had increased 2.8 fold over the number at 75 DAT. Very good control efficacies at 90 DAT occurred in all treatments with the combination of the fungus with an insecticide. At 105 DAT the numbers of nymphs had surged in all treatments, and no treatment provided effective control. The treatments with the highest earnings per hectare were A1 (3 × 10 12 M. a. conidia ha -1 + 65 g thiamethoxam) and M. anisopliae alone at the recommended dose of 3 × 10 12 M. a. conidia ha -1 . Our findings demonstrate the effectiveness of using either thiamethoxam or imidacloprid in combination with M. anisopliae to control M. fimbriolata nymphs on sugarcane, but greater net earnings per hectare occurred with the lowest rate of the thiamethoxam combination than with any of the imidacloprid combinations.Algunos insecticidas se puede utilizar con hongos entomopatógenos y por lo tanto, la aso- ciación de los controles químico y biológico puede ser una estrategia segura y eficaz para el control de insectos-plaga. El objetivo de este estudio fue evaluar los costos y eficacia de combinaciones de Metarhizium anisopliae (Metschnikoff) Sorokin (Hypocreales: Clavi- cipitaceae) con insecticidas thiamethoxam e imidacloprid para el control de la chicharrita (Mahanarva fimbriolata (Stål); Hemiptera: Cercopidae) en caña de azúcar . El experimento fue conducido en un delineamiento en bloques casualizados (DBC), con 10 tratamientos y 4 repeticiones. Los tratamientos que incluidos el control (sin tratamiento), thiamethoxam (250 g ha −1 ), imidacloprido (700 g ha −1 ), M. anisopliae (M.a.) (3×10 12 conidios ha −1 ), A1 (3×10 12 conidios ha −1 de M. a. + 65 g ha −1 de thiamethoxam), A2 (3×10 12 conidios ha −1 de M. a. + 125g ha −1 de thiamethoxam), A3 (3×10 12 conidios ha −1 de M. a. + 187.5 g ha −1 de thiamethoxam), A4 (3×10 12 conidios ha −1 de M.a + 175 g ha −1 de imidacloprido), A5 (3×10 12 conidios ha −1 de M. a. + 350 g ha −1 de imidacloprido) y A6 (3×10 12 conidios ha −1 de M. a. + 525g ha −1 de imidacloprido). Las reducciones en el número de ninfas M. fimbriolata por tratamiento en comparación con el control fueron similares a los 15 DAT (días pos tratamiento) en todos los tratamientos excepto A5 combinación (M. anisopliae y thiamethoxam). A los 30 DAT, el número de ninfas se redujeron significativamente en todos los tratamientos, excepto A3, y su eficacia varió de 14,28% para 92,85%. A los 45 DAT, los números de ninfas M. fimbriolata por tratamiento se redujeron significativamente en los siguientes tratamientos: imidacloprido solo en 700 g ha -1 , A1, A2, A3, A4 y A6; y las combinaciones de A1 y A2 causaron la más bajo infestaciones de ninfas M. fimbriolata y sus tasas de eficacia fueron de 77,41 y 87,09%, respectivamente. A los 75 DAT, los 2 mejores eficacias de control se produjeron en tratamientos A1 (3×10 12 conidios ha −1 de M. a. + 65 g ha −1 de thiamethoxam) y A5 (78.6%) (3×10 12 conidios ha −1 de M. a.+ 350 g ha −1 de imidacloprido). A los 90 DAT, el número de ninfas en el control había aumentado 2,8 veces más el número a 75 DAT. Muy buenas eficacias de control en 90 DAT, se produjo en todos los tratamientos con la combinación del hongo con un insecticida. A los 105 DAT, el número de ninfas habían aumentado en todos los tratamientos, y ningún tratamiento había proporcionado un control efectivo. Los tratamientos con los mayores rendimientos hectárea fueron A1 (3×10 12 conidios ha −1 de M. a.+ 65 g de thiamethoxam) y M. anisopliae solo a la dosis recomendada de 3×10 12 conidios ha −1 de M. a. Nuestros resultados demuestran la eficacia de thiamethoxam y imidacloprido en combinación con M. anisopliae para el control de ninfas M. fimbriolata en caña de azúcar, pero mayores beneficio neto por hectárea se produjeron con la tasa más baja de la combinación de thiamethoxam que con cualquiera de las combinaciones de imidacloprid

Aqueous and Methanolic Extracts of Caulerpa mexicana Suppress Cell Migration and Ear Edema Induced by Inflammatory Agents

The regulation of the inflammatory response is essential to maintaining homeostasis. Several studies have investigated new drugs that may contribute to avoiding or minimizing excessive inflammatory process. The aim of this study was to evaluate the effect of extracts of green algae Caulerpa mexicana on models inflammation. In mice, the inflammatory peritonitis model is induced by zymosan. Previous treatment of mice with aqueous and methanolic extracts of C. mexicana was able to suppress the cell migration to the peritoneal cavity, in a time-dependent but not in a dose-dependent manner. The treatment of mice with C. mexicana extracts also decreased the xylene-induced ear edema, exerting strong inhibitory leukocyte migration elicited by zymosan into the air pouch. We concluded that administration of the extracts resulted in a reduction of cell migration to different sites as well as a decrease in edema formation induced by chemical irritants. This study demonstrates for the first time the anti-inflammatory effect of aqueous and methanolic extracts from the green marine algae Caulerpa mexicana

The Antinociceptive and Anti-Inflammatory Activities of Caulerpin, a Bisindole Alkaloid Isolated from Seaweeds of the Genus Caulerpa

The antinociceptive and anti-inflammatory activity of caulerpin was investigated. This bisindole alkaloid was isolated from the lipoid extract of Caulerpa racemosa and its structure was identified by spectroscopic methods, including IR and NMR techniques. The pharmacological assays used were the writhing and the hot plate tests, the formalin-induced pain, the capsaicin-induced ear edema and the carrageenan-induced peritonitis. Caulerpin was given orally at a concentration of 100 μmol/kg. In the abdominal constriction test caulerpin showed reduction in the acetic acid-induced nociception at 0.0945 μmol (0.0103–1.0984) and for dypirone it was 0.0426 μmol (0.0092–0.1972). In the hot plate test in vivo the inhibition of nociception by caulerpin (100 μmol/kg, p.o.) was also favorable. This result suggests that this compound exhibits a central activity, without changing the motor activity (seen in the rotarod test). Caulerpin (100 μmol/kg, p.o.) reduced the formalin effects in both phases by 35.4% and 45.6%, respectively. The possible anti-inflammatory activity observed in the second phase in the formalin test of caulerpin (100 μmol/kg, p.o.) was confirmed on the capsaicin-induced ear edema model, where an inhibition of 55.8% was presented. Indeed, it was also observed in the carrageenan-induced peritonitis that caulerpin (100 μmol/kg, p.o.) exhibited anti-inflammatory activity, reducing significantly the number of recruit cells by 48.3%. Pharmacological studies are continuing in order to characterize the mechanism(s) responsible for the antinociceptive and anti-inflammatory actions and also to identify other active principles present in Caulerpa racemosa