84 research outputs found

    Covoiturage et territoire : quelle(s) proximité (s) dans la mobilité ?

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    International audienceCarpooling, as other shared uses for travelling, is growing for both economical and ideological reasons. Firstly related to long journey, this growth is now concerning average distance trips, especially in suburban areas. Suburbs are known for being automobile-oriented territories, structured by a sole travel mode, considered responsible for their unsustainability, their lack of social cohesion and functional efficiency. After exploratory research on the spatiality of social relations involved in shared use of cars, we are showing that carpooling jointly queried social cohesion and spatial organisation made in mobility. Producing new social and spatial convergences, these shared practices also reflect a capacity to create social organisations to face suburban liveability problems. These new uses suggest the possibility of “living together with and by networks” that questions suburban territoriality.Le covoiturage, comme d’autres modes de déplacement partagé, connaît un développement important, pour des raisons à la fois économiques et idéologiques. Concernant d’abord les trajets longue distance, cette croissance touche désormais ceux de moyenne distance, notamment au sein des espaces périurbains. Or ces espaces sont avant tout réputés pour les pratiques automobiles et individuelles qui les structurent, et ainsi mettent en cause leur durabilité, leur cohésion sociale et fonctionnelle. Suite à des recherches exploratoires sur la spatialité des rapports sociaux engagés dans les pratiques de co-déplacement, nous montrons que le covoiturage interroge conjointement la cohésion sociale et la structuration spatiale issue de la mobilité. Productrices de nouvelles convergences sociales et spatiales, ces pratiques témoignent d’une capacité d’organisation des acteurs sociaux face au problème de l’habiter périurbain. Elles suggèrent la possibilité d’un « vivre ensemble par et dans les réseaux » qui interpelle la question de la territorialité périurbaine

    Probing Spin Wave Diffraction Patterns of Curved Antennas

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    We report on the dependence of curvilinear shaped coplanar waveguides on the near-field diffraction patterns of spin waves propagating in perpendicularly magnetized thin films. Implementing the propagating spin waves spectroscopy techniques on either concentrically or eccentrically shaped antennas, we show how the link budget is directly affected by the spin wave interference, in good agreement with near-field diffraction simulations. This work demonstrates the feasibility to inductively probe a magnon interference pattern with a resolution down to 1μ\mum2^2, and provides a methodology for shaping spin wave beams from an antenna design. This methodology is successfully implemented in the case study of a spin wave Young's interference experiment

    Diatom molecular research comes of age: Model species for studying phytoplankton biology and diversity

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    Diatoms are the world’s most diverse group of algae, comprising at least 100,000 species. Contributing;20% of annual global carbon fixation, they underpin major aquatic food webs and drive global biogeochemical cycles. Over the past two decades, Thalassiosira pseudonana and Phaeodactylum tricornutum have become the most important model systems for diatom molecular research, ranging from cell biology to ecophysiology, due to their rapid growth rates, small genomes, and the cumulative wealth of associated genetic resources. To explore the evolutionary divergence of diatoms, additional model species are emerging, such as Fragilariopsis cylindrus and Pseudo-nitzschia multistriata. Here, we describe how functional genomics and reverse genetics have contributed to our understanding of this important class of microalgae in the context of evolution, cell biology, and metabolic adaptations. Our review will also highlight promising areas of investigation into the diversity of these photosynthetic organisms, including the discovery of new molecular pathways governing the life of secondary plastid-bearing organisms in aquatic environments

    Multisignal control of expression of the LHCX protein family in the marine diatom Phaeodactylum tricornutum

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    Diatoms are phytoplanktonic organisms that grow successfully in the ocean where light conditions are highly variable. Studies of the molecular mechanisms of light acclimation in the marine diatom Phaeodactylum tricornutum show that carotenoid de-epoxidation enzymes and LHCX1, a member of the light-harvesting protein family, both contribute to dissipate excess light energy through non-photochemical quenching (NPQ). In this study, we investigate the role of the other members of the LHCX family in diatom stress responses. Our analysis of available genomic data shows that the presence of multiple LHCX genes is a conserved feature of diatom species living in different ecological niches. Moreover, an analysis of the levels of four P. tricornutum LHCX transcripts in relation to protein expression and photosynthetic activity indicates that LHCXs are differentially regulated under different light intensities and nutrient starvation, mostly modulating NPQ capacity. We conclude that multiple abiotic stress signals converge to regulate the LHCX content of cells, providing a way to fine-tune light harvesting and photoprotection. Moreover, our data indicate that the expansion of the LHCX gene family reflects functional diversification of its members which could benefit cells responding to highly variable ocean environments

    Plastid thylakoid architecture optimizes photosynthesis in diatoms

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    Photosynthesis is a unique process that allows independent colonization of the land by plants and of the oceans by phytoplankton. Although the photosynthesis process is well understood in plants, we are still unlocking the mechanisms evolved by phytoplankton to achieve extremely efficient photosynthesis. Here, we combine biochemical, structural and in vivo physiological studies to unravel the structure of the plastid in diatoms, prominent marine eukaryotes. Biochemical and immunolocalization analyses reveal segregation of photosynthetic complexes in the loosely stacked thylakoid membranes typical of diatoms. Separation of photosystems within subdomains minimizes their physical contacts, as required for improved light utilization. Chloroplast 3D reconstruction and in vivo spectroscopy show that these subdomains are interconnected, ensuring fast equilibration of electron carriers for efficient optimum photosynthesis. Thus, diatoms and plants have converged towards a similar functional distribution of the photosystems although via different thylakoid architectures, which likely evolved independently in the land and the ocean.ISSN:2041-172

    Inactivation of genes coding for mitochondrial Nd7 and Nd9 complex I subunits in Chlamydomonas reinhardtii. Impact of complex I loss on respiration and energetic metabolism.

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    In Chlamydomonas, unlike in flowering plants, genes coding for Nd7 (NAD7/49kDa) and Nd9 (NAD9/30kDa) core subunits of mitochondrial respiratory-chain complex I are nucleus-encoded. Both genes possess all the features that facilitate their expression and proper import of the polypeptides in mitochondria. By inactivating their expression by RNA interference or insertional mutagenesis, we show that both subunits are required for complex I assembly and activity. Inactivation of complex I impairs the cell growth rate, reduces the respiratory rate, leads to lower intracellular ROS production and lower expression of ROS scavenging enzymes, and is associated to a diminished capacity to concentrate CO2 without compromising photosynthetic capacity.Peer reviewe

    Molecular and cellular mechanisms underlying the evolution of form and function in the amniote jaw.

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    The amniote jaw complex is a remarkable amalgamation of derivatives from distinct embryonic cell lineages. During development, the cells in these lineages experience concerted movements, migrations, and signaling interactions that take them from their initial origins to their final destinations and imbue their derivatives with aspects of form including their axial orientation, anatomical identity, size, and shape. Perturbations along the way can produce defects and disease, but also generate the variation necessary for jaw evolution and adaptation. We focus on molecular and cellular mechanisms that regulate form in the amniote jaw complex, and that enable structural and functional integration. Special emphasis is placed on the role of cranial neural crest mesenchyme (NCM) during the species-specific patterning of bone, cartilage, tendon, muscle, and other jaw tissues. We also address the effects of biomechanical forces during jaw development and discuss ways in which certain molecular and cellular responses add adaptive and evolutionary plasticity to jaw morphology. Overall, we highlight how variation in molecular and cellular programs can promote the phenomenal diversity and functional morphology achieved during amniote jaw evolution or lead to the range of jaw defects and disease that affect the human condition
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