Identification of the major tRNAPhe binding domain in the tetrameric structure of cytoplasmic phenylalanyl-tRNA synthetase from baker's yeast

AbstractNative cytoplasmic phenylalanyl-tRNA synthetase from baker's yeast is a tetramer of the α2β2 type. On mild tryptic cleavage it gives rise to a modified ∡2β′2 form that has lost the tRNAPhe binding capacity but is still able to activate phenylalanine. In this paper are presented data concerning peptides released by this limited proteolytic conversion as well as those arising from exhaustive tryptic digestion of the truncated β′ subunit. Each purified peptide was unambiguously assigned to a unique stretch of the β subunit amino acid sequence that was recently determined via gene cloning and DNA sequencing. Together with earlier results from affinity labelling studies the present data show that the Lys 172—Ile 173 bond is the unique target of trypsin under mild conditions and that the N-terminal domain of each β subunit (residues 1–172) contains the major tRNAPhe binding sites

HI in the Outskirts of Nearby Galaxies

The HI in disk galaxies frequently extends beyond the optical image, and can trace the dark matter there. I briefly highlight the history of high spatial resolution HI imaging, the contribution it made to the dark matter problem, and the current tension between several dynamical methods to break the disk-halo degeneracy. I then turn to the flaring problem, which could in principle probe the shape of the dark halo. Instead, however, a lot of attention is now devoted to understanding the role of gas accretion via galactic fountains. The current $\rm \Lambda$ cold dark matter theory has problems on galactic scales, such as the core-cusp problem, which can be addressed with HI observations of dwarf galaxies. For a similar range in rotation velocities, galaxies of type Sd have thin disks, while those of type Im are much thicker. After a few comments on modified Newtonian dynamics and on irregular galaxies, I close with statistics on the HI extent of galaxies.Comment: 38 pages, 17 figures, invited review, book chapter in "Outskirts of Galaxies", Eds. J. H. Knapen, J. C. Lee and A. Gil de Paz, Astrophysics and Space Science Library, Springer, in pres

Effective Lagrangians and Universality Classes of Nonlinear Bigravity

We discuss the fully non-linear formulation of multigravity. The concept of universality classes of effective Lagrangians describing bigravity, which is the simplest form of multigravity, is introduced. We show that non-linear multigravity theories can naturally arise in several different physical contexts: brane configurations, certain Kaluza-Klein reductions and some non-commutative geometry models. The formal and phenomenological aspects of multigravity (including the problems linked to the linearized theory of massive gravitons) are briefly discussed.Comment: 41 pages, 4 Figures, final version to be published in Phys.Rev.

Planck 2018 results. IV. Diffuse component separation

We present full-sky maps of the cosmic microwave background (CMB) and polarized synchrotron and thermal dust emission, derived from the third set of Planck frequency maps. These products have significantly lower contamination from instrumental systematic effects than previous versions. The methodologies used to derive these maps follow closely those described in earlier papers, adopting four methods (Commander, NILC, SEVEM, and SMICA) to extract the CMB component, as well as three methods (Commander, GNILC, and SMICA) to extract astrophysical components. Our revised CMB temperature maps agree with corresponding products in the Planck 2015 delivery, whereas the polarization maps exhibit significantly lower large-scale power, reflecting the improved data processing described in companion papers; however, the noise properties of the resulting data products are complicated, and the best available end-to-end simulations exhibit relative biases with respect to the data at the few percent level. Using these maps, we are for the first time able to fit the spectral index of thermal dust independently over 3 degree regions. We derive a conservative estimate of the mean spectral index of polarized thermal dust emission of beta_d = 1.55 +/- 0.05, where the uncertainty marginalizes both over all known systematic uncertainties and different estimation techniques. For polarized synchrotron emission, we find a mean spectral index of beta_s = -3.1 +/- 0.1, consistent with previously reported measurements. We note that the current data processing does not allow for construction of unbiased single-bolometer maps, and this limits our ability to extract CO emission and correlated components. The foreground results for intensity derived in this paper therefore do not supersede corresponding Planck 2015 products. For polarization the new results supersede the corresponding 2015 products in all respects

Planck 2018 results. III. High Frequency Instrument data processing and frequency maps

This paper presents the High Frequency Instrument (HFI) data processing procedures for the Planck 2018 release. Major improvements in mapmaking have been achieved since the previous 2015 release. They enabled the first significant measurement of the reionization optical depth parameter using HFI data. This paper presents an extensive analysis of systematic effects, including the use of simulations to facilitate their removal and characterize the residuals. The polarized data, which presented a number of known problems in the 2015 Planck release, are very significantly improved. Calibration, based on the CMB dipole, is now extremely accurate and in the frequency range 100 to 353 GHz reduces intensity-to-polarization leakage caused by calibration mismatch. The Solar dipole direction has been determined in the three lowest HFI frequency channels to within one arc minute, and its amplitude has an absolute uncertainty smaller than $0.35\mu$K, an accuracy of order $10^{-4}$. This is a major legacy from the HFI for future CMB experiments. The removal of bandpass leakage has been improved by extracting the bandpass-mismatch coefficients for each detector as part of the mapmaking process; these values in turn improve the intensity maps. This is a major change in the philosophy of "frequency maps", which are now computed from single detector data, all adjusted to the same average bandpass response for the main foregrounds. Simulations reproduce very well the relative gain calibration of detectors, as well as drifts within a frequency induced by the residuals of the main systematic effect. Using these simulations, we measure and correct the small frequency calibration bias induced by this systematic effect at the $10^{-4}$ level. There is no detectable sign of a residual calibration bias between the first and second acoustic peaks in the CMB channels, at the $10^{-3}$ level

Planck 2018 results. XII. Galactic astrophysics using polarized dust emission

We present 353 GHz full-sky maps of the polarization fraction p, angle \u3c8, and dispersion of angles S of Galactic dust thermal emission produced from the 2018 release of Planck data. We confirm that the mean and maximum of p decrease with increasing NH. The uncertainty on the maximum polarization fraction, pmax=22.0% at 80 arcmin resolution, is dominated by the uncertainty on the zero level in total intensity. The observed inverse behaviour between p and S is interpreted with models of the polarized sky that include effects from only the topology of the turbulent Galactic magnetic field. Thus, the statistical properties of p, \u3c8, and S mostly reflect the structure of the magnetic field. Nevertheless, we search for potential signatures of varying grain alignment and dust properties. First, we analyse the product map S 7p, looking for residual trends. While p decreases by a factor of 3--4 between NH=1020 cm 122 and NH=2 71022 cm 122, S 7p decreases by only about 25%, a systematic trend observed in both the diffuse ISM and molecular clouds. Second, we find no systematic trend of S 7p with the dust temperature, even though in the diffuse ISM lines of sight with high p and low S tend to have colder dust. We also compare Planck data with starlight polarization in the visible at high latitudes. The agreement in polarization angles is remarkable. Two polarization emission-to-extinction ratios that characterize dust optical properties depend only weakly on NH and converge towards the values previously determined for translucent lines of sight. We determine an upper limit for the polarization fraction in extinction of 13%, compatible with the pmax observed in emission. These results provide strong constraints for models of Galactic dust in diffuse gas

Planck 2018 results. XII. Galactic astrophysics using polarized dust emission

We present 353 GHz full-sky maps of the polarization fraction $p$, angle $\psi$, and dispersion of angles $S$ of Galactic dust thermal emission produced from the 2018 release of Planck data. We confirm that the mean and maximum of $p$ decrease with increasing $N_H$. The uncertainty on the maximum polarization fraction, $p_\mathrm{max}=22.0$% at 80 arcmin resolution, is dominated by the uncertainty on the zero level in total intensity. The observed inverse behaviour between $p$ and $S$ is interpreted with models of the polarized sky that include effects from only the topology of the turbulent Galactic magnetic field. Thus, the statistical properties of $p$, $\psi$, and $S$ mostly reflect the structure of the magnetic field. Nevertheless, we search for potential signatures of varying grain alignment and dust properties. First, we analyse the product map $S \times p$, looking for residual trends. While $p$ decreases by a factor of 3--4 between $N_H=10^{20}$ cm$^{-2}$ and $N_H=2\times 10^{22}$ cm$^{-2}$, $S \times p$ decreases by only about 25%, a systematic trend observed in both the diffuse ISM and molecular clouds. Second, we find no systematic trend of $S \times p$ with the dust temperature, even though in the diffuse ISM lines of sight with high $p$ and low $S$ tend to have colder dust. We also compare Planck data with starlight polarization in the visible at high latitudes. The agreement in polarization angles is remarkable. Two polarization emission-to-extinction ratios that characterize dust optical properties depend only weakly on $N_H$ and converge towards the values previously determined for translucent lines of sight. We determine an upper limit for the polarization fraction in extinction of 13%, compatible with the $p_\mathrm{max}$ observed in emission. These results provide strong constraints for models of Galactic dust in diffuse gas

Planck early results XXIV : Dust in the diffuse interstellar medium and the Galactic halo

Peer reviewe

Planck intermediate results. L. Evidence of spatial variation of the polarized thermal dust spectral energy distribution and implications for CMB B-mode analysis

The characterization of the Galactic foregrounds has been shown to be the main obstacle in thechallenging quest to detect primordial B-modes in the polarized microwave sky. We make use of the Planck-HFI 2015 data release at high frequencies to place new constraints on the properties of the polarized thermal dust emission at high Galactic latitudes. Here, we specifically study the spatial variability of the dust polarized spectral energy distribution (SED), and its potential impact on the determination of the tensor-to-scalar ratio, r. We use the correlation ratio of the CBBℓ angular power spectra between the 217 and 353 GHz channels as a tracer of these potential variations, computed on different high Galactic latitude regions, ranging from 80% to 20% of the sky. The new insight from Planck data is a departure of the correlation ratio from unity that cannot be attributed to a spurious decorrelation due to the cosmic microwave background, instrumental noise, or instrumental systematics. The effect is marginally detected on each region, but the statistical combination of all the regions gives more than 99% confidence for this variation in polarized dust properties. In addition, we show that the decorrelation increases when there is a decrease in the mean column density of the region of the sky being considered, and we propose a simple power-law empirical model for this dependence, which matches what is seen in the Planck data. We explore the effect that this measured decorrelation has on simulations of the BICEP2-Keck Array/Planck analysis and show that the 2015 constraints from these data still allow a decorrelation between the dust at 150 and 353 GHz that is compatible with our measured value. Finally, using simplified models, we show that either spatial variation of the dust SED or of the dust polarization angle are able to produce decorrelations between 217 and 353 GHz data similar to the values we observe in the data