Active probing of the mechanical properties of biological and synthetic vesicles

BACKGROUND: The interest in mechanics of synthetic and biological vesicles has been continuously growing during the last decades. Liposomes serve as model systems for investigating fundamental membrane processes and properties. More recently, extracellular vesicles (EVs) have been investigated mechanically as well. EVs are widely studied in fundamental and applied sciences, but their material properties remained elusive until recently. Elucidating the mechanical properties of vesicles is essential to unveil the mechanisms behind a variety of biological processes, e.g. budding, vesiculation and cellular uptake mechanisms. SCOPE OF REVIEW: The importance of mechanobiology for studies of vesicles and membranes is discussed, as well as the different available techniques to probe their mechanical properties. In particular, the mechanics of vesicles and membranes as obtained by nanoindentation, micropipette aspiration, optical tweezers, electrodeformation and electroporation experiments is addressed. MAJOR CONCLUSIONS: EVs and liposomes possess an astonishing rich, diverse behavior. To better understand their properties, and for optimization of their applications in nanotechnology, an improved understanding of their mechanical properties is needed. Depending on the size of the vesicles and the specific scientific question, different techniques can be chosen for their mechanical characterization. GENERAL SIGNIFICANCE: Understanding the mechanical properties of vesicles is necessary to gain deeper insight in the fundamental biological mechanisms involved in vesicle generation and cellular uptake. This furthermore facilitates technological applications such as using vesicles as targeted drug delivery vehicles. Liposome studies provide insight into fundamental membrane processes and properties, whereas the role and functioning of EVs in biology and medicine is increasingly elucidated

Phase Dependent Spectroscopy of Mira Variable Stars

Spectroscopic measurements of Mira variable stars, as a function of phase, probe the stellar atmospheres and underlying pulsation mechanisms. For example, measuring variations in TiO, VO, and ZrO with phase can be used to help determine whether these molecular species are produced in an extended region above the layers where Balmer line emission occurs or below this shocked region. Using the same methods, the Balmer-line increment, where the strongest Balmer line at phase zero is H-delta and not H-alpha can be measured and explanations tested, along with another peculiarity, the absence of the H-epsilon line in the spectra of Miras when other Balmer lines are strong. We present new spectra covering the spectral range from 6200 Angstroms to 9000 Angstroms of 20 Mira variables. A relationship between variations in the CaII IR triplet and H-alpha as a function of phase support the hypothesis that H-epsilon's observational characteristics result from an interaction of H-epsilon photons with the CaII H line. New periods and epochs of variability are also presented for each star

GPI-anchor signal sequence influences PrPC sorting, shedding and signalling, and impacts on different pathomechanistic aspects of prion disease in mice

The cellular prion protein (PrPC) is a cell surface glycoprotein attached to the membrane by a glycosylphosphatidylinositol (GPI)-anchor and plays a critical role in transmissible, neurodegenerative and fatal prion diseases. Alterations in membrane attachment influence PrPC-associated signaling, and the development of prion disease, yet our knowledge of the role of the GPI-anchor in localization, processing, and function of PrPC in vivo is limited We exchanged the PrPC GPI-anchor signal sequence of for that of Thy-1 (PrPCGPIThy-1) in cells and mice. We show that this modifies the GPI-anchor composition, which then lacks sialic acid, and that PrPCGPIThy-1 is preferentially localized in axons and is less prone to proteolytic shedding when compared to PrPC. Interestingly, after prion infection, mice expressing PrPCGPIThy-1 show a significant delay to terminal disease, a decrease of microglia/astrocyte activation, and altered MAPK signaling when compared to wild-type mice. Our results are the first to demonstrate in vivo, that the GPI-anchor signal sequence plays a fundamental role in the GPI-anchor composition, dictating the subcellular localization of a given protein and, in the case of PrPC, influencing the development of prion disease

Magnetic Fields in Large Diameter HII Regions Revealed by the Faraday Rotation of Compact Extragalactic Radio Sources

We present a study of the line-of-sight magnetic fields in five large-diameter Galactic HII regions. Using the Faraday rotation of background polarized radio sources, as well as dust-corrected H-alpha surface brightness as a probe of electron density, we estimated the strength and orientation of the magnetic field along 93 individual sight-lines through the HII regions. Each of the HII regions displayed a coherent magnetic field. The magnetic field strength (line-of-sight component) in the regions ranges from 2 to 6 microgauss, which is similar to the typical magnetic field strength in the diffuse interstellar medium. We investigated the relationship between magnetic field strength and electron density in the 5 HII regions. The slope of magnetic field vs. density in the low-density regime (0.8 < n_e < 30 per cubic cm) is very slightly above zero. We also calculated the ratio of thermal to magnetic pressure, beta_th, for each data point, which fell in the range 1.01 < beta_th < 25. Finally, we studied the orientation of the magnetic field in the solar neighborhood (d < 1.1 kpc) using our data from 5 HII regions along with existing measurements of the line-of-sight magnetic field strength from polarized pulsars whose distances have been determined from their annual parallax. We identify a net direction for the magnetic field in the solar neighborhood, but find no evidence for a preferred vertical direction of the magnetic field above or below the Galactic plane.Comment: Accepted to the Astrophysical Journal, June 4th 201

MaGICC discs: matching observed galaxy relationships over a wide stellar mass range

We use the same physical model to simulate four galaxies that match the relation between stellar and total mass, over a mass range that includes the vast majority of disc galaxies. The resultant galaxies, part of the Making Galaxies in a Cosmological Context (MaGICC) program, also match observed relations between luminosity, rotation velocity, size, colour, star formation rate, HI mass, baryonic mass, and metallicity. Radiation from massive stars and supernova energy regulate star formation and drive outflows, balancing the complex interplay between cooling gas, star formation, large scale outflows, and recycling of gas in a manner which correctly scales with the mass of the galaxy. Outflows also play a key role in simulating galaxies with exponential surface brightness profiles, flat rotation curves and dark matter cores. Our study implies that large scale outflows are the primary driver of the dependence of disc galaxy properties on mass. We show that the amount of outflows invoked in our model is required to meet the constraints provided by observations of OVI absorption lines in the circum-galactic-media of local galaxies

A distributed multiscale computation of a tightly coupled model using the Multiscale Modeling Language

AbstractNature is observed at all scales; with multiscale modeling, scientists bring together several scales for a holistic analysis of a phenomenon. The models on these different scales may require significant but also heterogeneous computational resources, creating the need for distributed multiscale computing. A particularly demanding type of multiscale models, tightly coupled, brings with it a number of theoretical and practical issues. In this contribution, a tightly coupled model of in-stent restenosis is first theoretically examined for its multiscale merits using the Multiscale Modeling Language (MML); this is aided by a toolchain consisting of MAPPER Memory (MaMe), the Multiscale Application Designer (MAD), and Gridspace Experiment Workbench. It is implemented and executed with the general Multiscale Coupling Library and Environment (MUSCLE). Finally, it is scheduled amongst heterogeneous infrastructures using the QCG-Broker. This marks the first occasion that a tightly coupled application uses distributed multiscale computing in such a general way

Clues on the origin of galactic angular momentum from looking at galaxy pairs

We search for correlations between the spin in pairs of spiral galaxies, to study if the angular momentum gain for each galaxy was the result of tidal torques imprint by the same tidal field. To perform our study we made use of a sample of galaxy pairs identified using the Sloan Digital Sky Survey. We find a weak, but statistically significant correlation between the spin magnitude of neighbouring galaxies, but no clear alignment between their orientation. We show that events such as interactions with close neighbours play an important role in the value of the spin for the final configuration, as we find these interactions tend to reduce the value of the $\lambda$ spin parameter of late-type galaxies considerably, with dependence on the morphology of the neighbour. This implies that the original tidal field for each pair could have been similar, but the redistribution of angular momentum at later stages of evolution is important.Comment: 10 pages, 4 figures, 1 table. Replaced to match the version accepted for publication in MNRA

"LUDO" - Kids playing Distributed Denial of Service

Distributed denial of service attacks pose a serious threat to the availability of the network infrastructures and services. GE̿ANT, the pan-European network with terabit capacities witnesses close to hundreds of DDoS attacks on a daily basis. The reason is that DDoS attacks are getting larger, more sophisticated and frequent. At the same time, it has never been easier to execute DDoS attacks, e.g., Booter services offer paying customers without any technical knowledge the possibility to perform DDoS attacks as a service. Given the increasing size, frequency and complexity of DDoS attacks, there is a need to perform a collaborative mitigation. Therefore, we developed (i) a DDoSDB to share real attack data and allow collaborators to query, compare, and download attacks, (ii) the Security attack experimentation framework to test mitigation and response capabilities and (iii) a collaborative mitigation and response process among trusted partners to disseminate security event information. In addition to these developments, we present and would like to discuss our latest research results with experienced networking operators and bridging the gap between academic research and operational business

Economic damages from on-going climate change imply deeper near-term emission cuts

Pathways toward limiting global warming to well below 2 ∘C, as used by the IPCC in the Fifth Assessment Report, do not consider the climate impacts already occurring below 2 ∘C. Here we show that accounting for such damages significantly increases the near-term ambition of transformation pathways. We use econometric estimates of climate damages on GDP growth and explicitly model the uncertainty in the persistence time of damages. The Integrated Assessment Model we use includes the climate system and mitigation technology detail required to derive near-term policies. We find an optimal carbon price of $115 per tonne of CO2 in 2030. The long-term persistence of damages, while highly uncertain, is a main driver of the near-term carbon price. Accounting for damages on economic growth increases the gap between the currently pledged nationally determined contributions and the welfare-optimal 2030 emissions by two thirds, compared to pathways considering the 2 ∘C limit only