High frequency winding design for planar switch mode transformers

Switch mode power supplies as a mass produced article leave room for optimization especially at the voluminous magnetic components. Hereby planar component designs can reduce the overall height of the power supply and give it a more compact design. Since cores are available in standard sizes the optimal winding layout has to be found. Due to increased parasitic capacitance interleaving is not a good option to reduce losses in the windings at high frequency resonant power supplies. This article presents and evaluates different winding designs for resonant switch mode transformers operating in power supplies with frequencies larger than 500kHz. This article shows that the revolved parallel design performs best of the tested designs under the given conditions. In addition, the revolved design can compete with solutions as planar litz when it comes to value meaning cost and production complexity. Furthermore, the article shows the abilities of a heat measurement prototype.reviewe

Análise da produção escrita em matemática, de alunos do ensino fundamental

Anais do II Seminário Seminário Estadual PIBID do Paraná: tecendo saberes / organizado por Dulcyene Maria Ribeiro e Catarina Costa Fernandes — Foz do Iguaçu: Unioeste; Unila, 2014Este trabalho contém reflexões a partir da produção escrita dos alunos do Ensino Fundamental de um colégio estadual da cidade de Cascavel. Foi aplicada por nosso grupo de trabalho do subprojeto de Matemática do PIBID aos alunos da escola uma avaliação composta por 13 questões, das quais algumas eram rotineiras e outras não rotineiras, retiradas e/ou adaptadas de provas aplicadas em nível nacional e internacional, como prova Brasil e PISA. Destas 13 questões escolhemos uma para fazermos algumas análises e compartilharmos a produção escrita dos alunos, as diferentes formas de resolução e nossas impressões a partir do que esperávamos que eles fizessem e sobre o que fizera

Análise da produção escrita em matemática, de alunos do ensino fundamental

Anais do II Seminário Seminário Estadual PIBID do Paraná: tecendo saberes / organizado por Dulcyene Maria Ribeiro e Catarina Costa Fernandes — Foz do Iguaçu: Unioeste; Unila, 2014Este trabalho contém reflexões a partir da produção escrita dos alunos do Ensino Fundamental de um colégio estadual da cidade de Cascavel. Foi aplicada por nosso grupo de trabalho do subprojeto de Matemática do PIBID aos alunos da escola uma avaliação composta por 13 questões, das quais algumas eram rotineiras e outras não rotineiras, retiradas e/ou adaptadas de provas aplicadas em nível nacional e internacional, como prova Brasil e PISA. Destas 13 questões escolhemos uma para fazermos algumas análises e compartilharmos a produção escrita dos alunos, as diferentes formas de resolução e nossas impressões a partir do que esperávamos que eles fizessem e sobre o que fizera

Introdução à modelagem matemática utilizando a construção de uma casa

Anais do II Seminário Seminário Estadual PIBID do Paraná: tecendo saberes / organizado por Dulcyene Maria Ribeiro e Catarina Costa Fernandes — Foz do Iguaçu: Unioeste; Unila, 2014Uma das teorias interessantes da Educação Matemática é a Modelagem Matemática. Este trabalho contém uma primeira aplicação desta teoria para o ensino em uma aula. São apresentadas algumas observações sobre esta experiência realizada com alunos do Ensino Fundamental de um colégio estadual da cidade de Cascavel por três acadêmicos do curso d e Licenciatura em Matemátic

Histone 3.3 hotspot mutations in conventional osteosarcomas: a comprehensive clinical and molecular characterization of six H3F3A mutated cases

Background: Histone 3.3 (H3.3) hotspot mutations in bone tumors occur in the vast majority of giant cell tumors of bone (GCTBs; 96%), chondroblastomas (95%) and in a few cases of osteosarcomas. However, clinical presentation, histopathological features, and additional molecular characteristics of H3.3 mutant osteosarcomas are largely unknown. Methods: In this multicentre, retrospective study, a total of 106 conventional high-grade osteosarcomas, across all age groups were re-examined for hotspot mutations in the H3.3 coding genes H3F3A and H3F3B. H3.3 mutant osteosarcomas were re-evaluated in a multidisciplinary manner and analyzed for genome-wide DNA-methylation patterns and DNA copy number aberrations alongside H3.3 wild-type osteosarcomas and H3F3A G34W/L mutant GCTBs. Results: Six osteosarcomas (6/106) carried H3F3A hotspot mutations. No mutations were found in H3F3B. All patients with H3F3A mutant osteosarcoma were older than 30 years with a median age of 65 years. Copy number aberrations that are commonly encountered in high-grade osteosarcomas also occurred in H3F3A mutant osteosarcomas. Unlike a single osteosarcoma with a H3F3A K27M mutation, the DNA methylation profiles of H3F3A G34W/R mutant osteosarcomas were clearly different from H3.3 wild-type osteosarcomas, but more closely related to GCTBs. The most differentially methylated promoters between H3F3A G34W/R mutant and H3.3 wild-type osteosarcomas were in KLLN/PTEN (p < 0.00005) and HIST1H2BB (p < 0.0005). Conclusions: H3.3 mutations in osteosarcomas may occur in H3F3A at mutational hotspots. They are overall rare, but become more frequent in osteosarcoma patients older than 30 years. Osteosarcomas carrying H3F3A G34W/R mutations are associated with epigenetic dysregulation of KLLN/PTEN and HIST1H2BB

Bacteria establish an aqueous living space in plants crucial for virulence

High humidity has a strong influence on the development of numerous diseases affecting the above-ground parts of plants (the phyllosphere) in crop fields and natural ecosystems, but the molecular basis of this humidity effect is not understood. Previous studies have emphasized immune suppression as a key step in bacterial pathogenesis. Here we show that humidity-dependent, pathogen-driven establishment of an aqueous intercellular space (apoplast) is another important step in bacterial infection of the phyllosphere. Bacterial effectors, such as Pseudomonas syringae HopM1, induce establishment of the aqueous apoplast and are sufficient to transform non-pathogenic P. syringae strains into virulent pathogens in immunodeficient Arabidopsis thaliana under high humidity. Arabidopsis quadruple mutants simultaneously defective in a host target (AtMIN7) of HopM1 and in pattern-triggered immunity could not only be used to reconstitute the basic features of bacterial infection, but also exhibited humidity-dependent dyshomeostasis of the endophytic commensal bacterial community in the phyllosphere. These results highlight a new conceptual framework for understanding diverse phyllosphere–bacterial interactions

Filariasis and lymphoedema

Among the causes of lymphoedema (LE), secondary LE due to filariasis is the most prevalent. It affects only a minority of the 120 million people infected with the causative organisms of lymphatic filariasis (LF), Wuchereria bancrofti and Brugia malayi/timori, but is clustered in families, indicating a genetic basis for development of this pathology. The majority of infected individuals develop filarial-specific immunosuppression that starts even before birth in cases where mothers are infected and is characterized by regulatory T-cell responses and high levels of IgG4, thus tolerating high parasite loads and microfilaraemia. In contrast, individuals with this pathology show stronger immune reactions biased towards Th1, Th2 and probably also Th17. Importantly, as for the aberrant lymph vessel development, innate immune responses that are triggered by the filarial antigen ultimately result in the activation of vascular endothelial growth factors (VEGF), thus promoting lymph vessel hyperplasia as a first step to lymphoedema development. Wolbachia endosymbionts are major inducers of these responses in vitro, and their depletion by doxycycline in LF patients reduces plasma VEGF and soluble VEGF-receptor-3 levels to those seen in endemic normals preceding pathology improvement. The search for the immunogenetic basis for LE could lead to the identification of risk factors and thus, to prevention; and has so far led to the identification of single-nucleotide polymorphisms (SNP) with potential functional relevance to VEGF, cytokine and toll-like receptor (TLR) genes. Hydrocele, a pathology with some similarity to LE in which both lymph vessel dilation and lymph extravasation are shared sequelae, has been found to be strongly associated with a VEGF-A SNP known for upregulation of this (lymph-)angiogenesis factor