Proof-of-concept trial of the combination of lactitol with Bifidobacterium bifidum and Lactobacillus acidophilus for the eradication of intestinal OXA-48-producing Enterobacteriaceae

Background: The major reservoir of carbapenemase-producing Enterobacteriaceae (CPE) is the gastrointestinal tract of colonized patients. Colonization is silent and may last for months, but the risk of infection by CPE in colonized patients is significant. Methods: Eight long-Term intestinal carriers of OXA-48-producing Enterobacteriaceae (OXA-PE) were treated during 3 weeks with daily oral lactitol (Emportal®), Bifidobacterium bifidum and Lactobacillus acidophilus (Infloran®). Weekly stool samples were collected during the treatment period and 6 weeks later. The presence of OXA-PE was investigated by microbiological cultures and qPCR. Results: At the end of treatment (EoT, secondary endpoint 1), four of the subjects had negative OXA-PE cultures. Three weeks later (secondary endpoint 2), six subjects were negative. Six weeks after the EoT (primary endpoint), three subjects had negative OXA-PE cultures. The relative intestinal load of OXA-PE decreased in all the patients during treatment. Conclusions: The combination of prebiotics and probiotics was well tolerated. A rapid reduction on the OXA-PE intestinal loads was observed. At the EoT, decolonization was achieved in three patients

The B. subtilis Rok protein is an atypical H-NS-like protein irresponsive to physico-chemical cues

Nucleoid-associated proteins (NAPs) play a central role in chromosome organization and environment-responsive transcription regulation. The Bacillus subtilis-encoded NAP Rok binds preferentially AT-rich regions of the genome, which often contain genes of foreign origin that are silenced by Rok binding. Additionally, Rok plays a role in chromosome architecture by binding in genomic clusters and promoting chromosomal loop formation. Based on this, Rok was proposed to be a functional homolog of E. coli H-NS. However, it is largely unclear how Rok binds DNA, how it represses transcription and whether Rok mediates environment-responsive gene regulation. Here, we investigated Rok's DNA binding properties and the effects of physico-chemical conditions thereon. We demonstrate that Rok is a DNA bridging protein similar to prototypical H-NS-like proteins. However, unlike these proteins, the DNA bridging ability of Rok is not affected by changes in physico-chemical conditions. The DNA binding properties of the Rok interaction partner sRok are affected by salt concentration. This suggests that in a minority of Bacillus strains Rok activity can be modulated by sRok, and thus respond indirectly to environmental stimuli. Despite several functional similarities, the absence of a direct response to physico-chemical changes establishes Rok as disparate member of the H-NS family.Macromolecular Biochemistr

Continuous GPS stations deployment in the Topo-Iberia Project framework

Topo-Iberia es un proyecto financiado por el Ministerio de Educación y Ciencia de España. Su principal objetivo es comprender las interacciones entre los procesos profundos, superficiales y atmosféricos integrando datos geológicos, geofísicos y geodéticos. El proyecto se centrará en tres zonas principales de interés en la Península Ibérica: los bordes N y S de la Placa Ibérica (incluyendo el N de Marruecos) y su núcleo central intermedio. Presentamos las actividades preliminares realizadas por el subgrupo GPS de Topo-Iberia con el fin de desplegar una nueva red continua de GPS. La intención es complementar las redes CGPS continuas existentes (p.e. ROA; ERGPS) incrementado la cobertura espacial en España y N de Marruecos. Una vez seleccionados los emplazamientos de las nuevas estaciones, el objetivo es que se encuentren en pleno funcionamiento la primavera de 2008. Se han descargado conjuntos de datos CGPS de servidores de distintas instituciones sobre los que se ha realizado un procesado inicial que sirva como control de calidad. Como algunas de las estaciones actualmente disponibles no siguen los estrictos procedimientos de estabilidad dictados por IGS/EUREF, estamos evaluando sus resultados mediante análisis de series temporales para decidir cuales se pueden incluir como estaciones complementarias de la red Topo-Iberia.Topo-Iberia is a Spanish Research Council funded project. Its main objective is to understand the interactions between deep, shallow and atmospheric processes, integrating geological, geophysical, geodetic and geo-technological research activities. The project will focus in three main areas of interest in the Iberian Peninsula: Northern and Southern borders of the Iberian micro-plate (taking into account the Northern part of Morocco), and its central core. We present the preliminary steps that the Topo- Iberia GPS working group is undergoing in order to deploy a new Continuous GPS Network. We are trying to complement the existing and functioning CGPS Networks (e.g. ROA; ERGPS) by increasing the spatial coverage across Spain and Northern Morocco. The places for the new locations have been chosen and all stations should be working in the spring 2008. A set of available CGPS data files has been downloaded from different institutional servers. A preliminary data analysis has been performed for geodetic quality control. Since some of the existing CGPS stations have been installed without following strict IGS/EUREF procedures for the stability of the monuments, we are evaluating their performance through the time series analysis, in order to decide whether to include them as complementing stations to our network.Depto. de Geodinámica, Estratigrafía y PaleontologíaFac. de Ciencias GeológicasTRUEMinisterio de Ciencia e Innovación (MICINN)CONSOLIDER TOPO-Iberiapu

7th Drug hypersensitivity meeting: part two

No abstract availabl

Measurement of the CKM angle γγ in B±→DK±B^\pm\to D K^\pm and B±→Dπ±B^\pm \to D π^\pm decays with D→KS0h+h−D \to K_\mathrm S^0 h^+ h^-

A measurement of $CP$-violating observables is performed using the decays $B^\pm\to D K^\pm$ and $B^\pm\to D \pi^\pm$, where the $D$ meson is reconstructed in one of the self-conjugate three-body final states $K_{\mathrm S}\pi^+\pi^-$ and $K_{\mathrm S}K^+K^-$ (commonly denoted $K_{\mathrm S} h^+h^-$). The decays are analysed in bins of the $D$-decay phase space, leading to a measurement that is independent of the modelling of the $D$-decay amplitude. The observables are interpreted in terms of the CKM angle $\gamma$. Using a data sample corresponding to an integrated luminosity of $9\,\text{fb}^{-1}$ collected in proton-proton collisions at centre-of-mass energies of $7$, $8$, and $13\,\text{TeV}$ with the LHCb experiment, $\gamma$ is measured to be $\left(68.7^{+5.2}_{-5.1}\right)^\circ$. The hadronic parameters $r_B^{DK}$, $r_B^{D\pi}$, $\delta_B^{DK}$, and $\delta_B^{D\pi}$, which are the ratios and strong-phase differences of the suppressed and favoured $B^\pm$ decays, are also reported

Whole-genome sequencing reveals host factors underlying critical COVID-19

Critical COVID-19 is caused by immune-mediated inflammatory lung injury. Host genetic variation influences the development of illness requiring critical care1 or hospitalization2,3,4 after infection with SARS-CoV-2. The GenOMICC (Genetics of Mortality in Critical Care) study enables the comparison of genomes from individuals who are critically ill with those of population controls to find underlying disease mechanisms. Here we use whole-genome sequencing in 7,491 critically ill individuals compared with 48,400 controls to discover and replicate 23 independent variants that significantly predispose to critical COVID-19. We identify 16 new independent associations, including variants within genes that are involved in interferon signalling (IL10RB and PLSCR1), leucocyte differentiation (BCL11A) and blood-type antigen secretor status (FUT2). Using transcriptome-wide association and colocalization to infer the effect of gene expression on disease severity, we find evidence that implicates multiple genes—including reduced expression of a membrane flippase (ATP11A), and increased expression of a mucin (MUC1)—in critical disease. Mendelian randomization provides evidence in support of causal roles for myeloid cell adhesion molecules (SELE, ICAM5 and CD209) and the coagulation factor F8, all of which are potentially druggable targets. Our results are broadly consistent with a multi-component model of COVID-19 pathophysiology, in which at least two distinct mechanisms can predispose to life-threatening disease: failure to control viral replication; or an enhanced tendency towards pulmonary inflammation and intravascular coagulation. We show that comparison between cases of critical illness and population controls is highly efficient for the detection of therapeutically relevant mechanisms of disease

Efecto de la balneoterapia en la mejora de la memoria en sujetos con estrés laboral

OriginalesEl propósito de este estudio fue valorar los efectos de la balneoterapia en la mejora de la atención y la memoria en un grupo de ejecutivos con estrés laboral. Para este fin hemos investigado a un grupo de 30 ejecutivos de grandes empresas con estrés laboral (12 mujeres y 18 hombres), con una media de edad de 48 +/- 10 años. Los sujetos han sido divididos en tres grupos, un grupo participó en un tratamiento de blaneoterapia, otro de relax mental y otro de balneoterapia combinada con estimulación cognitiva. Los resultados de este estudio permiten observar que la balneoterapia en combinación con estimulación cognitiva mejora en todas las pruebas cognitivas aplicadas, principalmente en la atención y la memoria de trabajo.N