Numerical analysis of shells. Volume 2 - User's manual for ''STARS-2'' - Shell Theory Automated for Rotational Structures-2, digital computer program

Imput information for application of Shell Theory Automated for Rotational Structures, /STARS 2/ digital computer program - Vol.

Numerical analysis of shells. Volume 3 - Engineer's program manual for ''STARS-2'' - Shell Theory Automated for Rotational Structures-2, digital computer program

Manual of engineering programming information for Shell Theory Automated for Rotational Structures /STARS 2/ - Vol.

Retinoic Acid promotes a totipotent-like stem cell through Zscan4 epigenetic activation

In culture, embryonic stem cells (ESCs) are a heterogeneous population that includes several cell intermediates with different degrees of potency. In particular, the subpopulation marked by Zscan4 expression, resembling molecular and epigenetic two-cell stage preimplantation signature, results enhanced after RA-treatment. We detected the epigenetic mechanisms involved in Zscan4 activation RA-dependent. We identified the minimal Zscan4 promoter region responsive to epigenetic stimuli, such as AZA and TSA, using transgenic ESCs stably transfected with different deletions of the Zscan4 promoter regions and their specific mutation. Thus, we identified a minimal promoter region responsive to epigenetics stimuli, demonstrating that Zscan4 activation is related to the histone acetylation status and DNA demethylation. This Zscan4 promoter region, at -300 bp to TSS, contains binding motifs for the DUX and TBX transcription factors and three specific CpG sites responsible for Zscan4 RA-activation. The next step will be identifying the proteins that bind and regulate the Zscan4 activation RA-induced by using a dCAS9 system, followed by a Mass Spec analysis

Preimaginal stages of Acostatrichia simulans Mosely 1939, a Neotropical microcaddisfly (Trichoptera: Hydroptilidae: Leucotrichiinae)

The preimaginal stages and cases of Acostatrichia simulans Mosely 1939, collected in Salto Encantado Provincial Park (Misiones province, Argentina) are described. This is the first description of the immature stages of this genus, and its first record from Argentina. The larvae were collected manually and transported to the laboratory, where they completed their development. Their identity was established using the metamorphotype method. They show strong resemblance to the larvae of Abtrichia Mosely, Anchitrichia Flint, Ceratotrichia Flint, Leucotrichia Mosely and Zumatrichia Mosely, especially to Zumatrichia.Fil: Angrisano, Elisa B.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; ArgentinaFil: Sganga, Julieta Valeria. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin