28 research outputs found
Metric to quantify white matter damage on brain magnetic resonance images
PURPOSE: Quantitative assessment of white matter hyperintensities (WMH) on structural Magnetic Resonance Imaging (MRI) is challenging. It is important to harmonise results from different software tools considering not only the volume but also the signal intensity. Here we propose and evaluate a metric of white matter (WM) damage that addresses this need. METHODS: We obtained WMH and normal-appearing white matter (NAWM) volumes from brain structural MRI from community dwelling older individuals and stroke patients enrolled in three different studies, using two automatic methods followed by manual editing by two to four observers blind to each other. We calculated the average intensity values on brain structural fluid-attenuation inversion recovery (FLAIR) MRI for the NAWM and WMH. The white matter damage metric is calculated as the proportion of WMH in brain tissue weighted by the relative image contrast of the WMH-to-NAWM. The new metric was evaluated using tissue microstructure parameters and visual ratings of small vessel disease burden and WMH: Fazekas score for WMH burden and Prins scale for WMH change. RESULTS: The correlation between the WM damage metric and the visual rating scores (Spearman Ï > =0.74, p  =0.72, p < 0.0001). The repeatability of the WM damage metric was better than WM volume (average median difference between measurements 3.26% (IQR 2.76%) and 5.88% (IQR 5.32%) respectively). The follow-up WM damage was highly related to total Prins score even when adjusted for baseline WM damage (ANCOVA, p < 0.0001), which was not always the case for WMH volume, as total Prins was highly associated with the change in the intense WMH volume (p = 0.0079, increase of 4.42 ml per unit change in total Prins, 95%CI [1.17 7.67]), but not with the change in less-intense, subtle WMH, which determined the volumetric change. CONCLUSION: The new metric is practical and simple to calculate. It is robust to variations in image processing methods and scanning protocols, and sensitive to subtle and severe white matter damage
Improving data availability for brain image biobanking in healthy subjects: practice-based suggestions from an international multidisciplinary working group
International audienceBrain imaging is now ubiquitous in clinical practice and research. The case for bringing together large amounts of image data from well-characterised healthy subjects and those with a range of common brain diseases across the life course is now compelling. This report follows a meeting of international experts from multiple disciplines, all interested in brain image biobanking. The meeting included neuroimaging experts (clinical and non-clinical), computer scientists, epidemiologists, clinicians, ethicists, and lawyers involved in creating brain image banks. The meeting followed a structured format to discuss current and emerging brain image banks; applications such as atlases; conceptual and statistical problems (e.g. defining 'normality'); legal, ethical and technological issues (e.g. consents, potential for data linkage, data security, harmonisation, data storage and enabling of research data sharing). We summarise the lessons learned from the experiences of a wide range of individual image banks, and provide practical recommendations to enhance creation, use and reuse of neuroimaging data. Our aim is to maximise the benefit of the image data, provided voluntarily by research participants and funded by many organisations, for human health. Our ultimate vision is of a federated network of brain image biobanks accessible for large studies of brain structure and function
Epigenomic profiling of preterm infants reveals DNA methylation differences at sites associated with neural function
DNA methylation (DNAm) plays a determining role in neural cell fate and provides a molecular link between early-life stress and neuropsychiatric disease. Preterm birth is a profound environmental stressor that is closely associated with alterations in connectivity of neural systems and long-term neuropsychiatric impairment. The aims of this study were to examine the relationship between preterm birth and DNAm, and to investigate factors that contribute to variance in DNAm. DNA was collected from preterm infants (birth<33 weeks gestation) and healthy controls (birth>37 weeks), and a genome-wide analysis of DNAm was performed; diffusion magnetic resonance imaging (dMRI) data were acquired from the preterm group. The major fasciculi were segmented, and fractional anisotropy, mean diffusivity and tract shape were calculated. Principal components (PC) analysis was used to investigate the contribution of MRI features and clinical variables to variance in DNAm. Differential methylation was found within 25 gene bodies and 58 promoters of protein-coding genes in preterm infants compared with controls; 10 of these have neural functions. Differences detected in the array were validated with pyrosequencing. Ninety-five percent of the variance in DNAm in preterm infants was explained by 23 PCs; corticospinal tract shape associated with 6th PC, and gender and early nutritional exposure associated with the 7th PC. Preterm birth is associated with alterations in the methylome at sites that influence neural development and function. Differential methylation analysis has identified several promising candidate genes for understanding the genetic/epigenetic basis of preterm brain injury
Effect of perinatal adversity on structural connectivity of the developing brain
Globally, preterm birth (defined as birth at <37 weeks of gestation) affects
around 11% of deliveries and it is closely associated with cerebral palsy,
cognitive impairments and neuropsychiatric diseases in later life.
Magnetic Resonance Imaging (MRI) has utility for measuring different
properties of the brain during the lifespan. Specially, diffusion MRI has been
used in the neonatal period to quantify the effect of preterm birth on white
matter structure, which enables inference about brain development and
injury.
By combining information from both structural and diffusion MRI, is it possible
to calculate structural connectivity of the brain. This involves calculating a
model of the brain as a network to extract features of interest. The process
starts by defining a series of nodes (anatomical regions) and edges
(connections between two anatomical regions). Once the network is created,
different types of analysis can be performed to find features of interest,
thereby allowing group wise comparisons.
The main frameworks/tools designed to construct the brain connectome have
been developed and tested in the adult human brain. There are several
differences between the adult and the neonatal brain: marked variation in
head size and shape, maturational processes leading to changes in signal
intensity profiles, relatively lower spatial resolution, and lower contrast
between tissue classes in the T1 weighted image. All of these issues make
the standard processes to construct the brain connectome very challenging
to apply in the neonatal population. Several groups have studied the neonatal
structural connectivity proposing several alternatives to overcome these
limitations.
The aim of this thesis was to optimise the different steps involved in
connectome analysis for neonatal data. First, to provide accurate parcellation
of the cortex a new atlas was created based on a control population of term
infants; this was achieved by propagating the atlas from an adult atlas
through intermediate childhood spatio-temporal atlases using image
registration. After this the advanced anatomically-constrained tractography
framework was adapted for the neonatal population, refined using software
tools for skull-stripping, tissue segmentation and parcellation specially
designed and tested for the neonatal brain. Finally, the method was used to
test the effect of early nutrition, specifically breast milk exposure, on
structural connectivity in preterm infants. We found that infants with higher
exposure to breastmilk in the weeks after preterm birth had improved
structural connectivity of developing networks and greater fractional
anisotropy in major white matter fasciculi. These data also show that the
benefits are dose dependent with higher exposure correlating with increased
white matter connectivity.
In conclusion, structural connectivity is a robust method to investigate the
developing human brain. We propose an optimised framework for the
neonatal brain, designed for our data and using tools developed for the
neonatal brain, and apply it to test the effect of breastmilk exposure on
preterm infants
Whole Brain Magnetic Resonance Image Atlases: A Systematic Review of Existing Atlases and Caveats for Use in Population Imaging
Brain MRI atlases may be used to characterize brain structural changes across the life course. Atlases have important applications in research, e.g., as registration and segmentation targets to underpin image analysis in population imaging studies, and potentially in future in clinical practice, e.g., as templates for identifying brain structural changes out with normal limits, and increasingly for use in surgical planning. However, there are several caveats and limitations which must be considered before successfully applying brain MRI atlases to research and clinical problems. For example, the influential Talairach and Tournoux atlas was derived from a single fixed cadaveric brain from an elderly female with limited clinical information, yet is the basis of many modern atlases and is often used to report locations of functional activation. We systematically review currently available whole brain structural MRI atlases with particular reference to the implications for population imaging through to emerging clinical practice. We found 66 whole brain structural MRI atlases world-wide. The vast majority were based on T1, T2, and/or proton density (PD) structural sequences, had been derived using parametric statistics (inappropriate for brain volume distributions), had limited supporting clinical or cognitive data, and included few younger (>5 and 60 years) subjects. To successfully characterize brain structural features and their changes across different stages of life, we conclude that whole brain structural MRI atlases should include: more subjects at the upper and lower extremes of age; additional structural sequences, including fluid attenuation inversion recovery (FLAIR) and T2* sequences; a range of appropriate statistics, e.g., rank-based or non-parametric; and detailed cognitive and clinical profiles of the included subjects in order to increase the relevance and utility of these atlases