Recomendações técnicas para o controle de pragas do pepino.

bitstream/item/71676/1/ct-1091.pd

Homologue expression of a fungal endo-1,4-β-Dxylanase using submerged and solid substrate fermentations

The xyn5 gene, which encodes an endo-β-1,4-xylanase (Xyn5), in Aspergillus niger GS1 was cloned into an expression cassette under the control of constitutive glyceraldhehyde-3-phosphate dehydrogenase gene promoter. The expression system was designed to produce the recombinant enzyme containing a six-histidine peptide fused to the carboxyl end of the protein. The efficiency of Xyn5 production under submerged (SmF) and solid-state (SSF) fermentation was investigated using the homologous cotransformed A. niger AB4.1. A productivity of 17.1 U/(l·h) was estimated for SSF and 3.2 U/(l·h) for SmF calculated at peak value of enzyme titers. Recombinant Xyn5 obtained by SSF on polyurethane fiber, was purified 5.1-fold by anion exchange and immobilized metal affinity chromatography, with 35.7% recovery. The purified recombinant enzyme showed an apparent molecular weight of 30 kDa and optimal activity (522 U/mg protein) at pH 5.5 and 50°C.Key words: Aspergillus niger GS1, xylanolytic activity, solid-state fermentation, homologue expression, polyurethane fiber

First observation of a narrow charm-strange meson DsJ(2632) -> Ds eta and D0 K+

We report the first observation of a charm-strange meson DsJ(2632) at a mass of 2632.6+/-1.6 MeV/c^2 in data from SELEX, the charm hadro-production experiment E781 at Fermilab. This state is seen in two decay modes, Ds eta and D0 K+. In the Ds eta decay mode we observe an excess of 49.3 events with a significance of 7.2sigma at a mass of 2635.9+/-2.9 MeV/c^2. There is a corresponding peak of 14 events with a significance of 5.3sigma at 2631.5+/-1.9 MeV/c^2 in the decay mode D0 K+. The decay width of this state is <17 MeV/c^2 at 90% confidence level. The relative branching ratio Gamma(D0K+)/Gamma(Dseta) is 0.16+/-0.06. The mechanism which keeps this state narrow is unclear. Its decay pattern is also unusual, being dominated by the Ds eta decay mode.Comment: 5 pages, 3 included eps figures. v2 as accepted for publication by PR

Confirmation of the Double Charm Baryon Xi_cc+ via its Decay to p D+ K-

We observes a signal for the double charm baryon Xi_cc+ in the charged decay mode Xi_cc+ -> p D+ K- to complement the previously reported decay Xi_cc+ -> Lambda_c K- pi+ in data from SELEX, the charm hadro-production experiment (E781) at Fermilab. In this new decay mode we observe an excess of 5.62 events over an expected background estimated by event mixing to be 1.38+/-0.13 events. The Poisson probability that a background fluctuation can produce the apparent signal is less than 6.4E-4. The observed mass of this state is (3518+/-3)MeV/c^2, consistent with the published result. Averaging the two results gives a mass of (3518.7+/-1.7)MeV/c^2. The observation of this new weak decay mode confirms the previous SELEX suggestion that this state is a double charm baryon. The relative branching ratio Gamma(Xi_cc+ -> pD+K-)/Gamma(Xi_cc+ -> Lambda_c K- pi+) = 0.36+/-0.21.Comment: 11 pages, 6 included eps figures. v2 includes improved statistical method to determine significance of observation. Submitted to PL

Persistence of COVID-19 Symptoms after Recovery in Mexican Population

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the coronavirus disease (COVID-19), a highly contagious infectious disease that has caused many deaths worldwide. Despite global efforts, it continues to cause great losses, and leaving multiple unknowns that we must resolve in order to face the pandemic more effectively. One of the questions that has arisen recently is what happens, after recovering from COVID-19. For this reason, the objective of this study is to identify the risk of presenting persistent symptoms in recovered from COVID-19. This case-control study was conducted in one state of Mexico. Initially the data were obtained from the participants, through a questionnaire about symptoms that they had at the moment of the interview. Initially were captured the collected data, to make a dataset. After the pre-processed using the R project tool to eliminate outliers or missing data. Obtained finally a total of 219 participants, 141 recovered and 78 controls. It was used confidence level of 90% and a margin of error of 7%. From results it was obtained that all symptoms have an associated risk in those recovered. The relative risk of the selected symptoms in the recovered patients goes from 3 to 22 times, being infinite for the case of dyspnea, due to the fact that there is no control that presents this symptom at the moment of the interview, followed by the nausea and the anosmia with a RR of 8.5. Therefore, public health strategies must be rethought, to treat or rehabilitate, avoiding chronic problems in patients recovered from COVID-19

An Enriched European Eel Transcriptome Sheds Light upon Host-Pathogen Interactions with Vibrio vulnificus

Infectious diseases are one of the principal bottlenecks for the European eel recovery. The aim of this study was to develop a new molecular tool to be used in host-pathogen interaction experiments in the eel. To this end, we first stimulated adult eels with different pathogen-associated molecular patterns (PAMPs), extracted RNA from the immune-related tissues and sequenced the transcriptome. We obtained more than 2 x 10(6) reads that were assembled and annotated into 45,067 new descriptions with a notable representation of novel transcripts related with pathogen recognition, signal transduction and the immune response. Then, we designed a DNA-microarray that was used to analyze the early immune response against Vibrio vulnificus, a septicemic pathogen that uses the gills as the portal of entry into the blood, as well as the role of the main toxin of this species (RtxA13) on this early interaction. The gill transcriptomic profiles obtained after bath infecting eels with the wild type strain or with a mutant deficient in rtxA13 were analyzed and compared. Results demonstrate that eels react rapidly and locally against the pathogen and that this immune-response is rtxA13-dependent as transcripts related with cell destruction were highly up-regulated only in the gills from eels infected with the wild-type strain. Furthermore, significant differences in the immune response against the wild type and the mutant strain also suggest that host survival after V. vulnificus infection could depend on an efficient local phagocytic activity. Finally, we also found evidence of the presence of an interbranchial lymphoid tissue in European eel gills although further experiments will be necessary to identify such tissue

The transverse momentum dependence of charged kaon Bose-Einstein correlations in the SELEX experiment

CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOWe report the measurement of the one-dimensional charged kaon correlation functions using 600GeV/c σ-, π- and 540GeV/c p beams from the SELEX (E781) experiment at the Fermilab Tevatron. K±K± correlation functions are studied for three transverse pair momentum, kT, ranges and parameterized by a Gaussian form. The emission source radii, R, and the correlation strength, λ, are extracted. The analysis shows a decrease of the source radii with increasing kaon transverse pair momentum for all beam types. © 2015 The Authors.We report the measurement of the one-dimensional charged kaon correlation functions using 600GeV/c σ-, π- and 540GeV/c p beams from the SELEX (E781) experiment at the Fermilab Tevatron. K±K± correlation functions are studied for three transverse pair momentum, kT, ranges and parameterized by a Gaussian form. The emission source radii, R, and the correlation strength, λ, are extracted. The analysis shows a decrease of the source radii with increasing kaon transverse pair momentum for all beam types.753458464CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOSem informaçãoSem informaçãoRuss, J.S., Akchurin, N., Andreev, V.A., First charm hadroproduction results from SELEX (1998) ICHEP'98 Proc. Int. Conf. on High Energy Physics II, p. 1259. , arxiv:hep-ex/9812031Goldhaber, G., Fowler, W.B., Goldhaber, S., Hoang, T.F., Kalogeropoulos, T.E., Powell, W.M., Pion-pion correlations in antiproton annihilation events (1959) Phys. Rev. Lett., 3, pp. 181-183Goldhaber, G., Goldhaber, S., Lee, W., Pais, A., Influence of Bose-Einstein statistics on the antiproton-proton annihilation process (1960) Phys. Rev., 120, pp. 300-312Kopylov, G.I., Podgoretsky, M.I., Correlations of identical particles emitted by highly excited nuclei (1972) Sov. J. Nucl. Phys., 15, pp. 219-223Kopylov, G.I., Podgoretsky, M.I., Multiple production and interference of particles emitted by moving sources (1974) Sov. J. Nucl. Phys., 18, pp. 336-341Kopylov, G.I., Like particle correlations as a tool to study the multiple production mechanism (1974) Phys. Lett. B, 50, pp. 472-474Abbiendi, G., Transverse and longitudinal Bose-Einstein correlations in hadronic Z0 decays (2000) Eur. Phys. J. C, 16, pp. 423-433. , arxiv:hep-ex/0002062Chekanov, S., Bose-Einstein correlations in one and two dimensions in deep inelastic scattering (2004) Phys. Lett. B, 583, pp. 231-246. , arxiv:hep-ex/0311030Aamodt, K., Two-pion Bose-Einstein correlations in pp collisions at s=900GeV (2010) Phys. Rev. D, 82. , arxiv:1007.0516Beker, H., MT dependence of boson interferometry in heavy ion collisions at the CERN SPS (1995) Phys. Rev. Lett., 74, pp. 3340-3343Adamczyk, L., Beam-energy-dependent two-pion interferometry and the freeze-out eccentricity of pions measured in heavy ion collisions at the STAR detector (2015) Phys. Rev. C, 92. , arxiv:1403.4972Adams, J., Experimental and theoretical challenges in the search for the quark-gluon plasma: the STAR Collaboration's critical assessment of the evidence from RHIC collisions (2005) Nucl. Phys. A, 757, pp. 102-183. , arxiv:nucl-ex/0501009Adcox, K., Formation of dense partonic matter in relativistic nucleus-nucleus collisions at RHIC: experimental evaluation by the PHENIX Collaboration (2005) Nucl. Phys. A, 757, pp. 184-283. , arxiv:nucl-ex/0410003Back, B.B., The PHOBOS perspective on discoveries at RHIC (2005) Nucl. Phys. A, 757, pp. 28-101. , arxiv:nucl-ex/0410022Arsene, I., Quark-gluon plasma and color glass condensate at RHIC? The perspective from the BRAHMS experiment (2005) Nucl. Phys. A, 757, pp. 1-27. , arxiv:nucl-ex/0410020Akkelin, S.V., Sinyukov, Y.M., The HBT-interferometry of expanding sources (1995) Phys. Lett. B, 356, pp. 525-530Alexander, G., Cohen, I., Levin, E., The dependence of the emission size on the hadron mass (1999) Phys. Lett. B, 452, pp. 159-166. , arxiv:hep-ph/9901341Kittel, W., Bose-Einstein correlations in Z fragmentation and other reactions (2001) Acta Phys. Pol. B, 32, pp. 3927-3972. , arxiv:hep-ph/0110088Chajȩcki, Z., Femtoscopy in hadron and lepton collisions: RHIC results and world systematics (2009) Acta Phys. Pol. B, 40, pp. 1119-1136. , arxiv:0901.4078Aggarwal, M.M., Pion femtoscopy in p+p collisions at s=200GeV (2011) Phys. Rev. C, 83. , arxiv:1004.0925Engelfried, J., The SELEX phototube RICH detector (1999) Nucl. Instrum. Methods A, 431, pp. 53-69. , arxiv:hep-ex/9811001Engelfried, J., The E781 (SELEX) RICH detector (1998) Nucl. Instrum. Methods A, 409, pp. 439-442Sjöstrand, T., Mrenna, S., Skands, P., PYTHIA 6.4 physics and manual (2006) J. High Energy Phys., 5. , arxiv:hep-ph/0603175v2Chajȩcki, Z., Lisa, M., Global conservation laws and femtoscopy of small systems (2008) Phys. Rev. C, 78. , arxiv:0803.0022Lednický, R., Lyuboshitz, V.L., Erazmus, B., Nouais, D., How to measure which sort of particles was emitted earlier and which later (1996) Phys. Lett. B, 373, pp. 30-34Voloshin, S., Lednický, R., Panitkin, S., Xu, N., Relative space-time asymmetries in pion and nucleon production in noncentral nucleus-nucleus collisions at high-energies (1997) Phys. Rev. Lett., 79, pp. 4766-4769. , arxiv:nucl-th/9708044Pratt, S., Shapes and sizes from non-identical-particle correlations (2007) Braz. J. Phys., 37, pp. 871-876. , arxiv:nucl-th/0612006Lednický, R., Finite-size effect on two-particle production (2008) J. Phys. G, Nucl. Part. Phys., 35Lednický, R., Lyuboshitz, V.V., Lyuboshitz, V.L., Final-state interactions in multichannel quantum systems and pair correlations of nonidentical and identical particles at low relative velocities (1998) Phys. At. Nucl., 61, pp. 2050-2063Bowler, M.G., Coulomb corrections to Bose-Einstein corrections have greatly exaggerated (1991) Phys. Lett. B, 270, pp. 69-74Sinyukov, Y., Lednický, R., Akkelin, S.V., Pluta, J., Erazmus, B., Coulomb corrections to Bose-Einstein corrections have greatly exaggerated (1998) Phys. Lett. B, 432, pp. 248-257Adams, J., Pion interferometry in Au+Au collisions at sNN=200GeV (2005) Phys. Rev. C, 71. , arxiv:nucl-ex/0411036Skands, P.Z., Tuning Monte Carlo generators: the Perugia tunes (2010) Phys. Rev. D, 82. , arxiv:1005.3457Abelev, B., Charged kaon femtoscopic correlations in pp collisions at s=7TeV (2013) Phys. Rev. D, 87. , arxiv:1212.5958v2Khachatryan, V., Measurement of Bose-Einstein correlations in pp collisions at s = 0.9 and 7 TeV (2011) J. High Energy Phys., 5. , arxiv:1101.3518Akkelin, S.V., Sinyukov, Y.M., Deciphering nonfemtoscopic two-pion correlations in p+p collisions with simple analytical models (2012) Phys. Rev. D, 85. , arxiv:1106.5120Lednický, R., Progulova, T.B., Influence of resonances on Bose-Einstein correlations of identical pions (1992) Z. Phys. C, 55, pp. 295-305Lisa, M., Pratt, S., Soltz, R., Wiedemann, U., Femtoscopy in relativistic heavy ion collisions: two decades of progress (2005) Annu. Rev. Nucl. Part. Sci., 55, pp. 357-402. , arxiv:nucl-ex/0505014Pratt, S., Pion interferometry for exploding sources (1984) Phys. Rev. Lett., 53, pp. 1219-1221Abbiendi, G., Bose-Einstein study of position-momentum correlations of charged pions in hadronic Z0 decays (2007) Eur. Phys. J. C, 52, pp. 787-803. , arxiv:0708.1122Achard, P., Test of the τ-model of Bose-Einstein correlations and reconstruction of the source function in hadronic Z-boson decay at LEP (2011) Eur. Phys. J. C, 71, p. 1648. , arxiv:1105.4788Aamodt, K., Femtoscopy of pp collisions at s = 0.9 and 7 TeV at the LHC with two-pion Bose-Einstein correlations (2011) Phys. Rev. D, 84. , arxiv:1101.3665Wiedemann, U.A., Heinz, U.W., Resonance contributions to Hanbury-Brown-Twiss correlation radii (1997) Phys. Rev. C, 56, pp. 3265-3286. , arxiv:nucl-th/9611031Werner, K., Karpenko, I., Pierog, T., Bleicher, M., Mikhailov, K., Evidence for hydrodynamic evolution in proton-proton scattering at 900 GeV (2011) Phys. Rev. C, 83. , arxiv:1010.0400Humanic, T.J., Predictions for two-pion correlations for s=14TeV proton-proton collisions (2007) Phys. Rev. C, 76. , arxiv:nucl-th/0612098Alexopoulos, T., Study of source size in pp- collisions at s=1.8TeV using pion interferometry (1993) Phys. Rev. D, 48, pp. 1931-1942Csorgo, T., Kittel, W., Metzger, W.J., Novák, T., Parametrization of Bose-Einstein correlations and reconstruction of the space-time evolution of pion production in e+e- annihilation (2008) Phys. Lett. B, 663, pp. 214-216. , arxiv:0803.3528Bialas, A., Kucharczyk, M., Palka, H., Zalewski, K., Mass dependence of HBT correlations in e+e- annihilation (2000) Phys. Rev. D, 62. , arxiv:hep-ph/0006290Alexander, G., Open questions related to Bose-Einstein correlations in e+e- → hadrons (2004) Acta Phys. Pol. B, 35, pp. 69-76. , arxiv:hep-ph/0311114Alexander, G., Mass and transverse mass effects on the hadron emitter size (2001) Phys. Lett. B, 506, pp. 45-51. , arxiv:hep-ph/0101319The authors are indebted to the staff of Fermi National Accelerator Laboratory and for invaluable technical support from the staffs of collaborating institutions. This project was supported in part by Bundesministerium für Bildung, Wissenschaft, Forschung und Technologie, Consejo Nacional de Ciencia y Tecnología (CONACyT), Conselho Nacional de Desenvolvimento Científico e Tecnológico, Fondo de Apoyo a la Investigación (UASLP), Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), the Israel Science Foundation founded by the Israel Academy of Sciences and Humanities, Istituto Nazionale di Fisica Nucleare (INFN), the International Science Foundation (ISF), the National Science Foundation, NATO, the Russian Academy of Sciences, the Russian Ministry of Science and Technology, the Russian Foundation for Basic Research (research project No. 11-02-01302-a), the Secretaría de Educación Pública (Mexico), the Turkish Scientific and Technological Research Board (TÜBİTAK), and the U.S. Department of Energy. We thank ITEP and National Research Nuclear University MEPhI (Moscow Engineering Physics Institute) for providing computing powers and support for data analysis and simulations. The authors also would like to thank Prof. Michael Lisa and Prof. Richard Lednický for helpful comments and fruitful discussions

Hippocampal CA3 Transcriptome Signature Correlates with Initial Precipitating Injury in Refractory Mesial Temporal Lobe Epilepsy

Background: Prolonged febrile seizures constitute an initial precipitating injury (IPI) commonly associated with refractory mesial temporal lobe epilepsy (RMTLE). in order to investigate IPI influence on the transcriptional phenotype underlying RMTLE we comparatively analyzed the transcriptomic signatures of CA3 explants surgically obtained from RMTLE patients with (FS) or without (NFS) febrile seizure history. Texture analyses on MRI images of dentate gyrus were conducted in a subset of surgically removed sclerotic hippocampi for identifying IPI-associated histo-radiological alterations.Methodology/Principal Findings: DNA microarray analysis revealed that CA3 global gene expression differed significantly between FS and NFS subgroups. An integrative functional genomics methodology was used for characterizing the relations between GO biological processes themes and constructing transcriptional interaction networks defining the FS and NFS transcriptomic signatures and its major gene-gene links (hubs). Co-expression network analysis showed that: i) CA3 transcriptomic profiles differ according to the IPI; ii) FS distinctive hubs are mostly linked to glutamatergic signalization while NFS hubs predominantly involve GABAergic pathways and neurotransmission modulation. Both networks have relevant hubs related to nervous system development, what is consistent with cell genesis activity in the hippocampus of RMTLE patients. Moreover, two candidate genes for therapeutic targeting came out from this analysis: SSTR1, a relevant common hub in febrile and afebrile transcriptomes, and CHRM3, due to its putative role in epilepsy susceptibility development. MRI texture analysis allowed an overall accuracy of 90% for pixels correctly classified as belonging to FS or NFS groups. Histological examination revealed that granule cell loss was significantly higher in FS hippocampi.Conclusions/Significance: CA3 transcriptional signatures and dentate gyrus morphology fairly correlate with IPI in RMTLE, indicating that FS-RMTLE represents a distinct phenotype. These findings may shed light on the molecular mechanisms underlying refractory epilepsy phenotypes and contribute to the discovery of novel specific drug targets for therapeutic interventions

First Observation of the Cabibbo-suppressed Decays Xi_c+ -> Sigma+ pi- pi+ and Xi_c+ -> Sigma- pi+ pi+ and Measurement of their Branching Ratios

We report the first observation of two Cabibbo-suppressed decay modes, Xi_c+ -> Sigma+ pi- pi+ and Xi_c+ -> Sigma- pi+ pi+. We observe 59+/-14 over a background of 87, and 22+/-8 over a background of 13 events, respectively, for the signals. The data were accumulated using the SELEX spectrometer during the 1996-1997 fixed target run at Fermilab, chiefly from a 600GeV/c Sigma- beam. The branching ratios of the decays relative to the Cabibbo--favored Xi_c+ -> Xi- pi+ pi+ are measured to be B(Xi_c+ -> Sigma+ pi- pi+)/B(Xi_c+ -> Xi- pi+ pi+) = 0.48+/-0.20, and B(Xi_c+ -> Sigma- pi+ pi+)/B(Xi_c+ -> Xi- pi+ pi+) = 0.18+/-0.09, respectively. We also report branching ratios for the same decay modes of the Lambda_c+ relative to Lambda_c+ -> p K- pi+.Comment: 15 pages, 5 figures, version 2 as accepted in PL