Superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression.

Aim: To evaluate the effects of preventive treatment with low-level laser therapy (LLLT) on progression of dystrophy in mdx mice. Methods: Ten animals were randomly divided into 2 experimental groups treated with superpulsed LLLT (904 nm, 15 mW, 700 Hz, 1 J) or placebo-LLLT at one point overlying the tibialis anterior muscle (bilaterally) 5 times per week for 14 weeks (from 6th to 20th week of age). Morphological changes, creatine kinase (CK) activity and mRNA gene expression were assessed in animals at 20th week of age. Results: Animals treated with LLLT showed very few morphological changes in skeletal muscle, with less atrophy and fibrosis than animals treated with placebo-LLLT. CK was significantly lower (p = 0.0203) in animals treated with LLLT (864.70 U.l−1, SEM 226.10) than placebo (1708.00 U.l−1, SEM 184.60). mRNA gene expression of inflammatory markers was significantly decreased by treatment with LLLT (p<0.05): TNF-α (placebo-control = 0.51 µg/µl [SEM 0.12], - LLLT = 0.048 µg/µl [SEM 0.01]), IL-1β (placebo-control = 2.292 µg/µl [SEM 0.74], - LLLT = 0.12 µg/µl [SEM 0.03]), IL-6 (placebo-control = 3.946 µg/µl [SEM 0.98], - LLLT = 0.854 µg/µl [SEM 0.33]), IL-10 (placebo-control = 1.116 µg/µl [SEM 0.22], - LLLT = 0.352 µg/µl [SEM 0.15]), and COX-2 (placebo-control = 4.984 µg/µl [SEM 1.18], LLLT = 1.470 µg/µl [SEM 0.73]). Conclusion: Irradiation of superpulsed LLLT on successive days five times per week for 14 weeks decreased morphological changes, skeletal muscle damage and inflammation in mdx mice. This indicates that LLLT has potential to decrease progression of Duchenne muscular dystrophy

Social job resources as sources of meaningfulness and its effects on nurses’ vigor and emotional exhaustion: a cross-sectional study among spanish nurses

This study investigates the mediating role of psychological meaningfulness among social job resources (i.e., coworkers and supervisor support), vigor, and emotional ex haustion in a nursing context. In spite of progress in under standing which organizational influence affects nurses’ vigor and emotional exhaustion, the psychological mechanisms as sumed to underlie the associations have not been fully ex plored. The sample for this study consisted of 171 nurses from Spanish hospitals (54.4 %) and Primary Care Centers (45.6 %). The mediation model was tested using the bootstrapping procedure. Our findings confirmed that psycho logical meaningfulness fully mediates the impact of social job resources on vigor at work. Moreover, psychological mean ingfulness partially mediated the impact of social job resources on emotional exhaustion. Results suggest that mean ingfulness plays an important role in the connection between job resources, vigor, and emotional exhaustion. The findings contribute to our understanding of the psychological processes that can explain how job resources contribute to the energetic aspect of burnout and engagement among nurses. Providing nurses with more social job resources, such as coworker and supervisor support, could activate their levels of personal meaningfulness and thus enhance their levels of well-being at workThis research was funded by an FPU grant from the Spanish Ministry of Education (AP2010-0099) and by a project from the Spanish Ministry of Economy and Competitiveness (FEM2012-34692

The plant WEE1 kinase is involved in checkpoint control activation in nematode-induced galls

Galls induced by plant‐parasitic nematodes involve a hyperactivation of the plant mitotic and endocycle machinery for their profit. Dedifferentiation of host root cells includes drastic cellular and molecular readjustments. In such background, potential DNA damage in the genome of gall cells is eminent. We questioned if DNA damage checkpoints activation followed by DNA repair occurred, or was eventually circumvented, in nematode‐induced galls. Galls display transcriptional activation of the DNA damage checkpoint kinase WEE1, correlated with its protein localization in the nuclei. The promoter of the stress marker gene SMR7 was evaluated under the WEE1‐knockout background. Drugs inducing DNA damage and a marker for DNA repair, PARP1 were used to understand mechanisms that might cope with DNA damage in galls. Our functional study revealed that gall cells lacking WEE1 conceivably entered mitosis prematurely disturbing the cell cycle despite the loss of genome integrity. The disrupted nuclei phenotype in giant cells hinted to the accumulation of mitotic defects. As well, WEE1‐knockout in Arabidopsis and downregulation in tomato repressed infection and reproduction of root‐knot nematodes. Together with data on DNA damaging drugs, we suggest a conserved function for WEE1 controlling a G1/S cell cycle arrest in response to replication defect in galls

Contribution to the development of product category rules for ceramic bricks

An Environmental Product Declaration (EPD) provides information on a product's environmental performance along its life cycle. This paper aims to contribute to the development of Product Category Rules (PCR) specific for ceramic bricks in order to support the establishment of a “cradle to grave” EPD. The methodology for developing the PCR takes into account ISO 14025, ISO 21930 and EN 15804, and the environmental profile is based on the Life Cycle Assessment (LCA) methodology. In this context, some core issues like product category definition, impact categories, indicators, cut-off criteria and allocation criteria are addressed. The selected impact categories for this study were: global warming, ozone layer depletion, photochemical oxidation, acidification, eutrophication, depletion of abiotic resources and respiratory inorganics. Indicators of energy and water consumption were also considered, as well as particle emissions to air. The results obtained from an LCA study on ceramic bricks produced in Portugal, to support the development of the PCR, show that the use of different fuels in the brick manufacturing stage has a significant effect in some impact categories. The use of petroleum coke generates higher impacts than natural gas or biomass. In general, the major environmental impacts occur in the brick manufacturing stage, mainly due to fuel usage in the firing operation. Particle emissions to air should be considered as an additional parameter in the EPD, being especially important when solid fuels are used. A sensitivity analysis of the cut-off criteria options was also conducted, which concluded that a 0.5% decrease in mass proved to be adequate for adoption, with a significant reduction in the effort required for data collection

Introducing the Green Protein Footprint method as an understandable measure of the environmental cost of anchovy consumption

In a global framework of growing concern for food security and environmental protection, the selection of food products with higher protein content and lower environmental impact is a challenge. To assess the reliability of different strategies along the food supply chain, a measure of food cost through the environmental impact-protein content binomial is necessary. This study proposes a standardized method to calculate the Green Protein Footprint (GPF) index, a method that assesses both the environmental impact of a food product and its protein content provided to consumers. Life Cycle Assessment (LCA) was used to calculate the environmental impact of the selected food products, and a Life Cycle Protein Assessment (LCPA) was performed by accounting for the protein content along the supply chain. Although the GPF can be applied to all food chain products, this paper is focused on European anchovy-based products for indirect human consumption (fishmeal) and for direct human consumption (fresh, salted and canned anchovies). Moreover, the circular economy concept was applied considering the valorization of the anchovy residues generated during the canning process. These residues were used to produce fishmeal, which was employed in bass aquaculture. Hence, humans are finally consuming fish protein from the residues, closing the loop of the original product life cycle. More elaborated, multi-ingredient food products (salted and canned anchovy products), presented higher GPF values due to higher environmental impacts. Furthermore, the increase of food loss throughout their life cycle caused a decrease in the protein content. Regarding salted and canned products, the packaging was the main hotspot. The influence of the packaging was evaluated using the GPF, reaffirming that plastic was the best alternative. These results highlighted the importance of improving packaging materials in food products.The authors thank the Ministry of Economy and Competitiveness of the Spanish Government for their financial support via the projects GeSAC-Conserva: Sustainable Management of the Cantabrian Anchovies (CTM2013-43539-R) and CERES_Project: Food Production strategies for climate change mitigation: towards a food circular economy (CTM 2016-76176-C2-1-R). Authors thank Julia Celaya for her technical support. Jara Laso thanks the Ministry of Economy and Competitiveness of Spanish Government for their financial support via the research fellowship BES-2014-069368

Red (660 nm) and infrared (830 nm) low-level laser therapy in skeletal muscle fatigue in humans: what is better?

In animal and clinical trials low-level laser therapy (LLLT) using red, infrared and mixed wavelengths has been shown to delay the development of skeletal muscle fatigue. However, the parameters employed in these studies do not allow a conclusion as to which wavelength range is better in delaying the development of skeletal muscle fatigue. With this perspective in mind, we compared the effects of red and infrared LLLT on skeletal muscle fatigue. A randomized double-blind placebo-controlled crossover trial was performed in ten healthy male volunteers. They were treated with active red LLLT, active infrared LLLT (660 or 830 nm, 50 mW, 17.85 W/cm2, 100 s irradiation per point, 5 J, 1,785 J/cm2 at each point irradiated, total 20 J irradiated per muscle) or an identical placebo LLLT at four points of the biceps brachii muscle for 3 min before exercise (voluntary isometric elbow flexion for 60 s). The mean peak force was significantly greater (p < 0.05) following red (12.14%) and infrared LLLT (14.49%) than following placebo LLLT, and the mean average force was also significantly greater (p < 0.05) following red (13.09%) and infrared LLLT (13.24%) than following placebo LLLT. There were no significant differences in mean average force or mean peak force between red and infrared LLLT. We conclude that both red than infrared LLLT are effective in delaying the development skeletal muscle fatigue and in enhancement of skeletal muscle performance. Further studies are needed to identify the specific mechanisms through which each wavelength acts

Model Consistency for Distributed Collaborative Modeling

International audienceCurrent collaborative modeling tools use a centralized architecture , based on version control system, where models are updated asynchronously. These tools depend on a single server and are not completely adapted for collaborative modeling, where update reactivity is essential. In this paper, we propose a framework for building collabo-rative modeling tools which provides synchronous model update. The framework is based on a peer-to-peer architecture and uses a consistency algorithm for model updating

Evapotranspiração e coeficientes de cultivo da beterraba orgânica sob cobertura morta de leguminosa e gramínea.

As práticas agrícolas que maximizam a produtividade e o uso da água são de vital importância para a agricultura. Assim, foram testados três tipos de manejo do solo com objetivo de determinar a evapotranspiração (ETc) e os coeficientes de cultivo (kc) da beterraba. Os tipos de manejo foram a utilização de coberturas mortas vegetais, denominadas capim cameroon (Pennisetum purpureum), gliricídia (Gliricidia sepium) e solo sem cobertura morta em área experimental do SIPA (Sistema Integrado de Produção Orgânica) localizado em Seropédica, Brasil. A lâmina de irrigação foi estimada com base no balanço de água no solo, cujo monitoramento foi realizado com a técnica da TDR. As ETc acumuladas para a cultura da beterraba foram 59,41; 55,31 e 119,62 mm, respectivamente, para capim cameroon, gliricídia e solo sem cobertura morta. A evapotranspiração de referência (ETo) foi obtida por meio do modelo de Penamn-Monteith. Os valores médios de kc obtidos para as fases inicial, média e final de desenvolvimento foram de 0,39; 0,42 e 1,02; 0,79; 0,76 e 1,18; e 0,56; 0,61 e 0,84, respectivamente, para capim cameroon, gliricídia e solo sem cobertura morta. O uso da cobertura do solo com gramínea ou leguminosa minimizou de forma expressiva a demanda hídrica da cultura da beterraba (Beta vulgaris)