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Adrenergic and mesenchymal signatures are identifiable in cell-free DNA and correlate with metastatic disease burden in children with neuroblastoma
Background: Cell-free DNA (cfDNA) profiles of 5-hydroxymethylcytosine (5-hmC), an epigenetic marker of open chromatin and active gene expression, are correlated with metastatic disease burden in patients with neuroblastoma. Neuroblastoma tumors are comprised of adrenergic (ADRN) and mesenchymal (MES) cells, and the relative abundance of each in tumor biopsies has prognostic implications. We hypothesized that ADRN and MES-specific signatures could be quantified in cfDNA 5-hmC profiles and would augment the detection of metastatic burden in patients with neuroblastoma. Methods: We previously performed an integrative analysis to identify ADRN and MES-specific genes (n = 373 and n = 159, respectively). Purified DNA from cell lines was serial diluted with healthy donor cfDNA. Using Gene Set Variation Analysis (GSVA), ADRN and MES signatures were optimized. We then quantified signature scores, and our prior neuroblastoma signature, in cfDNA from 84 samples from 46 high-risk patients including 21 patients with serial samples. Results: Samples from patients with higher metastatic burden had increased GSVA scores for both ADRN and MES gene signatures (p Conclusions: While it is feasible to identify ADRN and MES signatures using 5-hmC profiles of cfDNA from neuroblastoma patients and correlate these signatures to metastatic burden, additional data are needed to determine the optimal strategies for clinical implementation. Prospective evaluation in larger cohorts is ongoing.</p
Modalidades de enxertia para maracujazeiro: avaliação preliminar nas condições da Depressão Cuiabana
Representações sobre segurança alimentar e nutricional nos discursos de um Conselho de Alimentação Escolar
Este trabalho objetiva conhecer as representações sobre segurança alimentar e nutricional dos membros do Conselho de Alimentação Escolar de um município do oeste catarinense. O conceito de segurança alimentar e nutricional consiste na realização do direito de todos ao acesso regular e permanente a alimentos de qualidade, em quantidade suficiente, sem comprometer o acesso a outras necessidades essenciais, tendo como base práticas alimentares promotoras da saúde, que respeitem a diversidade cultural e que sejam ambiental, cultural, econômica e socialmente sustentáveis. O Programa Nacional de Alimentação Escolar é uma das mais importantes estratégias adotadas pelo Governo Federal com vistas à melhoria das condições de segurança alimentar e nutricional da população escolar. Os Conselhos de Alimentação Escolar são estruturas flexíveis de gestão dessa política pública, pela via do controle social. Este se trata de um estudo transversal qualitativo, que adota o referencial teórico-metodológico da análise de conteúdo e da teoria das representações sociais. São entrevistados todos os membros do conselho municipal de alimentação escolar (n=14). As falas são agrupadas em categorias, sendo as mais citadas: higiene (n=12), qualidade nutricional (n=8), aceitabilidade (n=4) e acesso regular (n=3). As categorias sustentabilidade e direito à alimentação não são citadas. A configuração do conselho sugere pouca representatividade. Os resultados indicam a necessidade de qualificação dos conselhos no sentido da construção de uma participação social comprometida e de uma compreensão ampliada sobre segurança alimentar e nutricional, de forma a contribuir para o exercício de um controle social efetivo e sintonizado com a política de alimentação escolar.This study aimed to investigate the representations on food security of the members of a School Feeding Board in a city located in the western region of the State of Santa Catarina. The concept of Food Security consists in the fulfillment of the right of all to regular and permanent access to high-quality food, in sufficient quantity, without compromising the access to other essential needs, based on health promoting food practices that respect cultural diversity and which are environmentally, culturally, economically and socially sustainable. The National School Feeding Program is one of the most important strategies adopted by the Federal Government to improve food security conditions of students. The School Feeding Boards are flexible structures for the management of this public policy by means of social control. This is a cross-sectional study with a qualitative approach which adopted the theoretical and methodological framework of content analysis and of social representations theory. All the members of the School Feeding Board (n=14) were interviewed. The answers were grouped into categories, and the most cited ones were: hygiene (n=12), nutritional quality (n=8), acceptability (n=4) and regular access (n=3). The categories sustainability and right to food were not mentioned by the interviewees. The board's configuration suggested little representation. The results indicate the need for qualification of the boards in order to construct a committed social participation and an expanded understanding about food security, so as to contribute to the exercise of effective social control, in tune with the school feeding policy
Propagação da corticeira do banhado (Erythrina crista-galli L.) (FABACEAE) pelo processo de estaquia
The Fifteenth Data Release of the Sloan Digital Sky Surveys: First Release of MaNGA-derived Quantities, Data Visualization Tools, and Stellar Library
Twenty years have passed since first light for the Sloan Digital Sky Survey (SDSS). Here, we release data taken by the fourth phase of SDSS (SDSS-IV) across its first three years of operation (2014 July–2017 July). This is the third data release for SDSS-IV, and the 15th from SDSS (Data Release Fifteen; DR15). New data come from MaNGA—we release 4824 data cubes, as well as the first stellar spectra in the MaNGA Stellar Library (MaStar), the first set of survey-supported analysis products (e.g., stellar and gas kinematics, emission-line and other maps) from the MaNGA Data Analysis Pipeline, and a new data visualization and access tool we call "Marvin." The next data release, DR16, will include new data from both APOGEE-2 and eBOSS; those surveys release no new data here, but we document updates and corrections to their data processing pipelines. The release is cumulative; it also includes the most recent reductions and calibrations of all data taken by SDSS since first light. In this paper, we describe the location and format of the data and tools and cite technical references describing how it was obtained and processed. The SDSS website (www.sdss.org) has also been updated, providing links to data downloads, tutorials, and examples of data use. Although SDSS-IV will continue to collect astronomical data until 2020, and will be followed by SDSS-V (2020–2025), we end this paper by describing plans to ensure the sustainability of the SDSS data archive for many years beyond the collection of data
Recommended from our members
The Fifteenth Data Release of the Sloan Digital Sky Surveys: First Release of MaNGA-derived Quantities, Data Visualization Tools, and Stellar Library
Twenty years have passed since first light for the Sloan Digital Sky Survey (SDSS). Here, we release data taken by the fourth phase of SDSS (SDSS-IV) across its first three years of operation (2014 July–2017 July). This is the third data release for SDSS-IV, and the 15th from SDSS (Data Release Fifteen; DR15). New data come from MaNGA—we release 4824 data cubes, as well as the first stellar spectra in the MaNGA Stellar Library (MaStar), the first set of survey-supported analysis products (e.g., stellar and gas kinematics, emission-line and other maps) from the MaNGA Data Analysis Pipeline, and a new data visualization and access tool we call "Marvin." The next data release, DR16, will include new data from both APOGEE-2 and eBOSS; those surveys release no new data here, but we document updates and corrections to their data processing pipelines. The release is cumulative; it also includes the most recent reductions and calibrations of all data taken by SDSS since first light. In this paper, we describe the location and format of the data and tools and cite technical references describing how it was obtained and processed. The SDSS website (www.sdss.org) has also been updated, providing links to data downloads, tutorials, and examples of data use. Although SDSS-IV will continue to collect astronomical data until 2020, and will be followed by SDSS-V (2020–2025), we end this paper by describing plans to ensure the sustainability of the SDSS data archive for many years beyond the collection of data
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