p53 Family: Role of Protein Isoforms in Human Cancer

TP53, TP63, and TP73 genes comprise the p53 family. Each gene produces protein isoforms through multiple mechanisms including extensive alternative mRNA splicing. Accumulating evidence shows that these isoforms play a critical role in the regulation of many biological processes in normal cells. Their abnormal expression contributes to tumorigenesis and has a profound effect on tumor response to curative therapy. This paper is an overview of isoform diversity in the p53 family and its role in cancer

Scorzonera sensu lato (Asteraceae, Cichorieae) – taxonomic reassessment in the light of new molecular phylogenetic and carpological analyses

Scorzonera comprises 180–190 species and belongs to the subtribe Scorzonerinae. Its circumscription has long been the subject of debate and available molecular phylogenetic analyses affirmed the polyphyly of Scorzonera in its wide sense. We provide a re-evaluation of Scorzonera and other related genera, based on carpological (including anatomical) and extended molecular phylogenetic analyses. We present, for the first time, a comprehensive sampling, including Scorzonera in its widest sense and all other genera recognised in the Scorzonerinae. We conducted phylogenetic analyses using Maximum Parsimony, Maximum Likelihood and Bayesian analyses, based on sequences of the nuclear ribosomal ITS and of two plastid markers (partial rbcL and matK) and Maximum Parsimony for reconstructing the carpological character states at ancestral nodes. Achene characters, especially related to pericarp anatomy, such as general topography of the tissue types, disposition of the mechanical tissue and direction of its fibres, presence or absence of air cavities, provide, in certain cases, support for the phylogenetic lineages revealed. Confirming the polyphyly of Scorzonera, we propose a revised classification of the subtribe, accepting the genera Scorzonera (including four major clades: Scorzonera s. str., S. purpurea, S. albicaulis and Podospermum), Gelasia, Lipschitzia gen. nov. (for the Scorzonera divaricata clade), Pseudopodospermum, Pterachaenia (also including Scorzonera codringtonii), Ramaliella gen. nov. (for the S. polyclada clade) and Takhtajaniantha. A key to the revised genera and a characterisation of the genera and major clades are provided

A new endemic species of Sesuvium (Aizoaceae: Sesuvioideae) from the Caribbean Basin, with further notes on the genus composition in the West Indies

A distinctive new species, Sesuvium curassavicum Sukhor. (Aizoaceae: Sesuvioideae), restricted to the Caribbean Basin (Kingdom of the Netherlands: Curacao and Bonaire Islands, North Colombia: La Guajira Department, and North Venezuela: Falcon State), is described and illustrated. It differs from all other perennial species growing in the West Indies by its papillate stems and wrinkled seeds. Based on the molecular phylogenetic analysis of nrDNA (ITS) and three plastid regions (rps16 gene intron, atpB-rbcL and trnL-trnF intergenic spacers), S. curassavicum is included in the 'American' clade, but its relationships are not fully resolved. The samples of the plants known as S. microphyllum fall within the 'Sesuvium portulacastrum' clade, and for that reason this species is considered here as a synonym of S. portulacastrum being an ecological form of the latter species. Sesuvium revolutifolium, S. ortegae and S. revolutum, described from cultivated plants are established as earlier synonyms of S. verrucosum, for which S. revolutifolium has priority and is proposed here as the correct name. These three species names seem to share the same provenance which cannot be Cuba, as stated in the protologue for S. revolutifolium, but rather Mexico. The name Sesuvium sessile is discussed and merged with S. portulacastrum. A new diagnostic key to the Sesuvium species in the West Indies is provided. In total, we accept for the West Indies the following species: S. curassavicum, S. humifusum, S. maritimum, S. portulacastrum and S. rubriflorum. The origins of collections of the neotype of Radiana petiolata and the holotypes of Sesuvium microphyllum and S. spathulatum are clarified.Peer reviewe

Study of the relationship between psycho-emotional wellbeing and personal qualities in Ukrainian youth: Ways of overcoming

Objectives. There is enough information about personal state and characteristics of the students from different countries, but about Ukrainian Senior school pupils and university students is completely missing. For the first time, the issue of stressful psychosomatic consequences for Ukrainian youth health is raised in world research. Methods. To assess wellbeing, anxiety, value orientations and behavior types in 216 youth (school/university participants) four standardized Instruments were used. Additionally, with other young/adult participants (n = 307), the method of conversation was used in order to establish their reaction to the ongoing Russian military aggression in Ukraine, and to find out the ways for prevention-rehabilitation the psycho-emotional state of all participants. Results and Conclusion. The greater anxiety, the more often competitive behavior. Wellbeing is negatively associated with competition behavior; anxiety is positively linked with adaptation. The youngest participants showed the worst state of health, higher anxiety and cooperative behavior. During the war unleashed by Russia, Ukrainians, on the one hand, were strongly motivated, aimed at the speedy cessation of hostilities which strengthened, encouraged them psycho-emotionally, on the other hand, needed special psychological support and help. High value orientations are combined with high conflict resolution. Special preventive-recovery activities were carried out with different categories of the population: refugees, people left in the war zone and their families, militaries, rescuers, medical personnel. Limitations. Findings on the anxiety and wellbeing interconnected with value orientations require clarification. Correctional-rehabilitation work efficiency provided to the participants suffered because of the hostilities need experimental-statistical confirmation

A decade of shaping the futures of polar early career researchers: A legacy of the International Polar Year

The Association of Polar Early Career Scientists (APECS) is an important legacy of the International Polar Year (IPY). APECS continues to foster engagement in education, outreach and communication (EOC) activities relating to the polar regions and provide training for early career researchers (ECRs). We highlight opportunities for training, leadership and skills development, such as the annual Polar Weeks and Antarctica Day celebrations. Participation and engagement in EOC activities actively contributes to career development by enabling ECRs to develop valuable soft skills such as networking, communication and interdisciplinary knowledge. A pilot survey on EOC engagement highlighted that those who organise events also gain leadership skills such as team management. We discuss several factors contributing to the success of APECS in training the next generation of polar leaders. These include the geographical rather than discipline-specific focus of the organisation, utilisation of online resources, including social media, and the strong links with partner organisations. These examples demonstrate how the EOC legacy of IPY has continued due to APECS’ targeted efforts to create EOC opportunities and provide skills and leadership training for ECRs

M‐type K⁺ channels in peripheral nociceptive pathways

Pathological pain is a hyperexcitability disorder. Since the excitability of a neuron is set and controlled by a complement of ion channels it expresses, in order to understand and treat pain, we need to develop a mechanistic insight into the key ion channels controlling excitability within the mammalian pain pathways and how these ion channels are regulated and modulated in various physiological and pathophysiological settings. In this review, we will discuss the emerging data on the expression in pain pathways, functional role and modulation of a family of voltage‐gated K⁺ channels called ‘M channels’ (KCNQ, Kv7). M channels are increasingly recognized as important players in controlling pain signalling, especially within the peripheral somatosensory system. We will also discuss the therapeutic potential of M channels as analgesic drug targets

The complete genome sequence of Corynebacterium pseudotuberculosis FRC41 isolated from a 12-year-old girl with necrotizing lymphadenitis reveals insights into gene-regulatory networks contributing to virulence

Trost E, Ott L, Schneider J, et al. The complete genome sequence of Corynebacterium pseudotuberculosis FRC41 isolated from a 12-year-old girl with necrotizing lymphadenitis reveals insights into gene-regulatory networks contributing to virulence. BMC Genomics. 2010;11(1): 728

THE ISOLATION, PROPERTIES AND PRACTICAL APPLICATION OF SOME THERMOSTABLE FERMENTS OF THE NUCLEIC ACIDS EXCHANGE

The object of investigation: fungus Spicaria violacea, bacterium Themus aquaticus. The purpose of the work: cleaning of the thermostable nucleases and DNA-polymerase Taq, creating the technology of receiving the 5'-desoxyribonucleotides. The nucleases, received from fungus Spicaria violacea have been isolated; the physico-chemical properties of the ferments and the efficiency of interaction of the DNA-polymerase with the primers of the different length have been studied. Created has been the technology for obtaining the 5'-desoxyribonucleotides; the methods of isolation of the DNA-polymerase Taq have been developed. The method of obtaining the 5'-desoxyribonucleotides and the DNA-polymerase Taq has been introduced in production. The field of application: obtaining the components of the nucleic acids and thermostable fermentsAvailable from VNTIC / VNTIC - Scientific & Technical Information Centre of RussiaSIGLERURussian Federatio