La casa-torre en Guipúzcoa

En número dedicado a: Guipúzco

Oral administration of a new copper (I) complex with coumarin as ligand: modulation of the immune response and the composition of the intestinal microbiota in Onchorhynchus mykiss

[Cu(NN1)2]ClO4 is a copper (I) complex, where NN1 is an imine ligand 6-((quinolin-2-ylmethylene) amino)-2H-chromen-2-one obtained by derivatization of natural compound coumarin, developed for the treatment of infectious diseases that affect salmonids. In previous research, we showed that the Cu(I) coordination complex possesses antibacterial activity against Flavobacterium psychrophilum, providing protection against this pathogen in rainbow trout during challenge assays (with an RPS of 50%). In the present study, the effects of administering [Cu(NN1)2]ClO4 to Oncorhynchus mykiss over a 60-days period were evaluated with regard to systemic immune response and its potential to alter intestinal microbiota composition. In O. mykiss, an immunostimulatory effect was evident at days 30 and 45 after administration, resulting in an increment of transcript levels of IFN-γ, IL-12, TNF-α, lysozyme and perforin. To determine whether these immunomodulatory effects correlated with changes in the intestinal microbiota, we analyzed the metagenome diversity by V4 16S rRNA sequencing. In O. mykiss, both [Cu(NN1)2]ClO4 and commercial antibiotic florfenicol had comparable effects at the phylum level, resulting in a predominance of proteobacteria and firmicutes. Nonetheless, at the genus level, florfenicol and [Cu(NN1)2]ClO4 complex exhibited distinct effects on the intestinal microbiota of O. mykiss. In conclusion, our findings demonstrate that [Cu(NN1)2]ClO4 is capable of stimulating the immune system at a systemic level, while inducing alterations in the composition of the intestinal microbiota in O. mykiss

Environmental evolution of the Basque Coast Geopark estuaries (southern Bay of Biscay) during the last 10,000 years

In order to reconstruct the environmental evolution of the Deba and Urola estuaries located in the Basque Coast Geopark at millennial, centennial and decadal timescales, four long boreholes, three short cores and twelve surface samples were studied. Multiproxy analysis (foraminifera, trace metals and radioisotopes) shows the temporal transformation of these estuaries in response to regional driving forces such as fresh-water discharge, relative sea-level (RSL) variation and the more recent impact of industrial development. At millennial and centennial timescales, the Deba estuary transformed from a tide-dominated to a river-dominated estuary at about 8000 yr cal BP following the decrease in RSL rise rate. This decrease also led to a reduction in both salinity and marine influence in the nearby tide-dominated Urola estuary. At decadal timescale, human disturbance on foraminiferal populations was found to be lower in the Deba estuary despite its higher level of contaminants in sediments. This was due to the greater impact of fresh-water discharge. In the Urola estuary, dredging operations altered severely the foraminiferal biota.Funding: This research was supported financially by Geoparkea-UPV/EHU (US13/02), Spanish MINECO (CGL2013-41083-P and RTI2018-095678-B-C21, MCIU/AEI/FEDER, UE), UPV/EHU (UFI11/09) and EJ/GV (IT365-10, IT767-13 and IT976-16) projects. NEA is supported by the Centre for Arctic Gas Hydrate, Environment and Climate (CAGE), the Research Council of Norway through its Centres of Excellence scheme (grant 223259). These funding sources were not involved in the study design, the collection, analysis and interpretation of data, the writing of the report, or the decision to submit the article for publication

Generación de patrones de costura a medida mediante Kinect y Makehuman

This document corresponds to the End of Bachelor's Project Report (Memoria del Proyecto de Fin de Grado), Generation of sewing patterns using the Kinect and MakeHuman, developed for the Faculty of Computer Engineering at the UPV-EHU university of Donostia-San Sebastián under Blanca Cases supervision, teacher at UPV-EHU University. The project consists in obtaining the measurements of someone by using the Kinect's depth sensor (which was previously achieved by Ibon Olabarria using only the image that ended up causing precision loss), creating the model with the client's form and measurements on MakeHuman and, after selecting the clothes, adapting the pattern automatically to the user's measurements. This would generate a life-size sewing pattern in a pdf.

Stability of Antibacterial Silver Carboxylate Complexes against Staphylococcus epidermidis and Their Cytotoxic Effects

The antibacterial effects against Staphylococcus epidermidis of five silver carboxylate complexes with anti-inflammatory ligands were studied in order to analyze and compare them in terms of stability (in solution and after exposure to UV light), and their antibacterial and morphological differences. Four effects of the Ag-complexes were evidenced by transmission electronic microscopy (TEM) and scanning electronic microscopy (SEM): DNA condensation, membrane disruption, shedding of cytoplasmic material and silver compound microcrystal penetration of bacteria. 5-Chlorosalicylic acid (5Cl) and sodium 4-aminosalicylate (4A) were the most effective ligands for synthesizing silver complexes with high levels of antibacterial activity. However, Ag-5Cl was the most stable against exposure UV light (365 nm). Cytotoxic effects were tested against two kinds of eukaryotic cells: murine fibroblast cells (T10 1/2) and human epithelial ovarian cancer cells (A2780). The main objective was to identify changes in their antibacterial properties associated with potential decomposition and the implications for clinical applications

Generación de patrones de costura a medida mediante Kinect y Makehuman

This document corresponds to the End of Bachelor's Project Report (Memoria del Proyecto de Fin de Grado), Generation of sewing patterns using the Kinect and MakeHuman, developed for the Faculty of Computer Engineering at the UPV-EHU university of Donostia-San Sebastián under Blanca Cases supervision, teacher at UPV-EHU University. The project consists in obtaining the measurements of someone by using the Kinect's depth sensor (which was previously achieved by Ibon Olabarria using only the image that ended up causing precision loss), creating the model with the client's form and measurements on MakeHuman and, after selecting the clothes, adapting the pattern automatically to the user's measurements. This would generate a life-size sewing pattern in a pdf.

New Copper(I) complex with a coumarin as ligand with antibacterial activity againstFlavobacterium psychrophilum

A new copper (I) complex, [Cu(NN1)(2)](ClO4)(,)was synthesized, where NN(1)was a imine ligand 6-((quinolin-2-ylmethylene)amino)-2H-chromen-2-one obtained by a derivatization of natural compound coumarin. The structural characterization in solution was done by NMR techniques, UV-Vis and cyclic voltammetry. The potential antibacterial effect of [Cu(NN1)(2)](ClO4)(,)was assessed forF. psychrophilumisolated 10094.F. psychrophilumis a Gram-negative bacterium which causes diseases such as bacterial cold-water disease and rainbow trout fry syndrome, causing large economic losses in the freshwater salmonid aquaculture industry. This complex show to have antibacterial activity againstF. psychrophilum10094 at non-cytotoxic concentration in cell line derived from trout (F. psychrophilum10094 IC(50)16.0 +/- 0.9; RT-GUT IC(50)53.0 +/- 3.1 mu g/mL).Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) CONICYT FONDECYT 1180265 1191902 Internal Doctoral Grant USACH FONDEQUIP EQM15010

Evaluation of the Antiviral Activity against Infectious Pancreatic Necrosis Virus (IPNV) of a Copper (I) Homoleptic Complex with a Coumarin as Ligand

The aquatic infectious pancreatic necrosis virus (IPNV) causes a severe disease in farmed salmonid fish that generates great economic losses in the aquaculture industry. In the search for new tools to control the disease, in this paper we show the results obtained from the evaluation of the antiviral effect of [Cu(NN1)2](ClO4) Cu(I) complex, synthesized in our laboratory, where the NN1 ligand is a synthetic derivate of the natural compound coumarin. This complex demonstrated antiviral activity against IPNV at 5.0 and 15.0 µg/mL causing a decrease viral load 99.0% and 99.5%, respectively. The Molecular Docking studies carried out showed that the copper complex would interact with the VP2 protein, specifically in the S domain, altering the process of entry of the virus into the host cell