103 research outputs found

    Kukin parhaan kykynsÀ mukaan:johtajuuteen liittyvÀt roolit ja kokemukset tieto- ja informaatioalan palveluja tuottavissa organisaatioissa

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    TiivistelmĂ€. TĂ€ssĂ€ pro gradu -tutkielmassa tarkastelen tieto- ja informaatioalan palveluja tuottavissa organisaatioissa harjoitettua esimiestyötĂ€ siihen kuuluvien tehtĂ€vien ja roolien, sekĂ€ esimiesten henkilökohtaisten kokemusten kautta. Tavoitteenani on tutkia, minkĂ€laisten kĂ€ytĂ€nnön toimien ja roolien varaan johtajuus rakentuu — ja millaisia ajatuksia sekĂ€ tunteita johtajuus aseman harjoittajissa herĂ€ttÀÀ. KerĂ€sin tutkimuksen laadullisen aineiston puolistrukturoiduilla teemahaastatteluilla kevĂ€ttalven 2017 aikana. Haastateltavia oli yhteensĂ€ kahdeksan kirjastoalan esimiestĂ€ eri puolella Suomea. Teemoittelin litteroidun haastatteluaineiston sisĂ€llönanalyysin vaikutteita ja temaattista analyysia hyödyntĂ€en seuraaviin kategorioihin: haastateltavan tausta ja työhistoria, johtajaksi pÀÀsy, työn sisĂ€ltö, siinĂ€ esiintyvĂ€t ongelmat, työn parhaat puolet sekĂ€ johtajuus. Viimeksi mainittuun kokonaisuuteen liittyvĂ€t haastateltavien kĂ€sitykset hyvĂ€stĂ€ ja huonosta johtajuudesta, sekĂ€ johtajalta vaadittavat ominaisuudet. Tuloksista ilmeni, ettĂ€ työhön liittyvĂ€t roolit ja tehtĂ€vĂ€kokonaisuudet vastaavat pitkĂ€lti Fayolin esityksiĂ€ johtajan työtehtĂ€vistĂ€, sekĂ€ Mintzbergin mÀÀrittelemiĂ€ perustehtĂ€viĂ€ ja rooleja pÀÀtöksentekoon, henkilösuhteisiin ja tietoon liittyvissĂ€ roolikokonaisuuksissa. Johtamistyön pyrkimyksenĂ€ on taata organisaation kilpailukyky, palvelujen sĂ€ilyvyys ja menestyvĂ€ kehitys kaikilla toiminnan osa-alueilla. TĂ€ltĂ€ osin nykypĂ€ivĂ€n johtajuus vastaa verrattain hyvin vanhoja malleja. Kun nĂ€itĂ€ mÀÀritelmiĂ€ verrataan kerÀÀmÀÀni aineistoon, voidaan johtajan työhön kuuluvista rooleista muodostaa laajempia toiminnallisia kokonaisuuksia. NĂ€in syntyivĂ€t yhdistĂ€jĂ€n ja mahdollistajan sekĂ€ luotsaajan, sekĂ€ ratkaisijan roolit. MitĂ€ roolien sisĂ€ltöön tulee, johtajan työ on monipuolista ja useita eri tehtĂ€viĂ€ kĂ€sittĂ€vÀÀ. TĂ€llöin työpĂ€ivĂ€n sisĂ€llön ennakoiminen on haastavaa — vaikkakin jonkinlainen vuosikello on mahdollista hahmottaa. TehtĂ€vistĂ€ korostuvat arjen ongelmanratkaisun lisĂ€ksi resursointi, budjetointi ja henkilöstöhallinnolliset vastuut sekĂ€ vuorovaikutus ja suhteiden hoito. Suurimpia haasteita ovat henkilöstöresurssien riittĂ€vyys sekĂ€ eri vastuiden vĂ€lisen tasapainon löytĂ€minen. Huomattavaa kuitenkin on, ettĂ€ merkittĂ€vĂ€ osa haastateltavista vastusti ongelma-termin kĂ€yttöÀ työn haasteista puhuttaessa. Haastavat tilanteet ovat arjen realiteetti, jotka tĂ€ytyy hyvĂ€ksyĂ€ ja ratkaista jÀÀmĂ€ttĂ€ niihin kiinni. LisĂ€ksi johtajan asema on usein luontevaksi koettu, toisinaan jopa tavoiteltu tehtĂ€vĂ€. Kaiken kaikkiaan haastateltavat nĂ€kevĂ€t työn vaativana, mutta antoisana. Moni haastateltava kuvailikin nauttivansa erityisesti työn ihmislĂ€heisyydestĂ€, ratkaisujen löytĂ€misestĂ€ ja asioiden viemisestĂ€ eteenpĂ€in. Kehitys ja muutos ovat voimavara, joka voidaan nĂ€hdĂ€ innostavana osana työtĂ€. Jatkotutkimuksella voitaisiin selvittÀÀ, pĂ€tevĂ€tkö nĂ€mĂ€ tulokset laajemmassakin mittakaavassa. Muita mahdollisia aiheita ovat alaisten nĂ€kemykset johtajan roolista, sekĂ€ alaisten ja esimiesten johtajuuteen liittĂ€mien kĂ€sitysten vertaileminen

    The Effect of Biochar Addition on Thermal Stability and Decomposition Mechanism of Poly(butylene succinate) Bionanocomposites

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    In the present study, poly(butylene succinate) (PBSu) and its bionanocomposites containing 1, 2.5, and 5 wt.% biochar (MSP700) were prepared via in situ melt polycondensation in order to investigate the thermal stability and decomposition mechanism of the materials. X-ray photoelectron spectroscopy (XPS) measurements were carried out to analyze the surface area of a biochar sample and PBSu/biochar nanocomposites. From XPS, it was found that only physical interactions were taking place between PBSu matrix and biochar nanoadditive. Thermal stability, decomposition kinetics, and the decomposition mechanism of the pristine PBSu and PBSu/biochar nanocomposites were thoroughly studied by thermogravimetric analysis (TGA) and pyrolysis–gas chromatography/mass spectrometry (Py−GC/MS). TGA thermograms depicted that all materials had high thermal stability, since their decomposition started at around 300 °C. However, results indicated a slight reduction in the thermal stability of the PBSu biochar nanocomposites because of the potential catalytic impact of biochar. Py−GC/MS analysis was employed to examine, in more detail, the thermal degradation mechanism of PBSu nanocomposites filled with biochar. From the decomposition products identified by Py−GC/MS after pyrolysis at 450 °C, it was found that the decomposition pathway of the PBSu/biochar nanocomposites took place mainly via ÎČ-hydrogen bond scission, which is similar to that which took place for neat PBSu. However, at higher biochar content (5 wt.%), some localized differences in the intensity of the peaks of some specific thermal degradation products could be recognized, indicating that α-hydrogen bond scission was also taking place. A study of the thermal stability and decomposition pathway of PBSu/biochar bionanocomposites is crucial to examine if the new materials fulfill the requirements for further investigation for mulch films in agriculture or in electronics as possible applications

    The Tumor Suppressor CYLD Inhibits Mammary Epithelial to Mesenchymal Transition by the Coordinated Inhibition of YAP/TAZ and TGFÎČ Signaling

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    Downregulation of the cylindromatosis (CYLD) tumor suppressor has been associated with breast cancer development and progression. Here, we report a critical role for CYLD in maintaining the phenotype of mammary epithelial cells in vitro and in vivo. CYLD downregulation or inactivation induced an epithelial to mesenchymal transition of mammary epithelial cells that was dependent on the concomitant activation of the transcription factors Yes-associated protein (YAP)/transcriptional coactivator with PDZ-binding motif (TAZ) and transforming growth factor beta (TGFïżœ)signaling. CYLD inactivation enhanced the nuclear localization of YAP/TAZ and the phosphorylation of Small Mothers Against Decapentaplegic (SMAD)2/3 proteins in confluent cell culture conditions. Consistent with these findings were the hyperplastic alterations of CYLD-deficient mouse mammary epithelia, which were associated with enhanced nuclear expression of the YAP/TAZ transcription factors. Furthermore, in human breast cancer samples, downregulation of CYLD expression correlates with enhanced YAP/TAZ-regulated target gene expression. Our results identify CYLD as a critical regulator of a signaling node that prevents the coordinated activation of YAP/TAZ and the TGFïżœ pathway in mammary epithelial cells, in order to maintain their phenotypic identity and homeostasis. Consequently, they provide a novel conceptual framework that supports and explains a causal implication of deficient CYLD expression in aggressive human breast cancers

    A systems model for immune cell interactions unravels the mechanism of inflammation in human skin

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    Inflammation is characterized by altered cytokine levels produced by cell populations in a highly interdependent manner. To elucidate the mechanism of an inflammatory reaction, we have developed a mathematical model for immune cell interactions via the specific, dose-dependent cytokine production rates of cell populations. The model describes the criteria required for normal and pathological immune system responses and suggests that alterations in the cytokine production rates can lead to various stable levels which manifest themselves in different disease phenotypes. The model predicts that pairs of interacting immune cell populations can maintain homeostatic and elevated extracellular cytokine concentration levels, enabling them to operate as an immune system switch. The concept described here is developed in the context of psoriasis, an immune-mediated disease, but it can also offer mechanistic insights into other inflammatory pathologies as it explains how interactions between immune cell populations can lead to disease phenotypes. © 2010 Valeyev et al

    Author Correction: Enhanced NF-ÎșB signaling in type-2 dendritic cells at baseline predicts non-response to adalimumab in psoriasis.

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    Funder: Department of HealthBiologic therapies have transformed the management of psoriasis, but clinical outcome is variable leaving an unmet clinical need for predictive biomarkers of response. Here we perform in-depth immunomonitoring of blood immune cells of 67 patients with psoriasis, before and during therapy with the anti-TNF drug adalimumab, to identify immune mediators of clinical response and evaluate their predictive value. Enhanced NF-ÎșBp65 phosphorylation, induced by TNF and LPS in type-2 dendritic cells (DC) before therapy, significantly correlates with lack of clinical response after 12 weeks of treatment. The heightened NF-ÎșB activation is linked to increased DC maturation in vitro and frequency of IL-17+ T cells in the blood of non-responders before therapy. Moreover, lesional skin of non-responders contains higher numbers of dermal DC expressing the maturation marker CD83 and producing IL-23, and increased numbers of IL-17+ T cells. Finally, we identify and clinically validate LPS-induced NF-ÎșBp65 phosphorylation before therapy as a predictive biomarker of non-response to adalimumab, with 100% sensitivity and 90.1% specificity in an independent cohort. Our study uncovers important molecular and cellular mediators underpinning adalimumab mechanisms of action in psoriasis and we propose a blood biomarker for predicting clinical outcome

    Finding and sharing: new approaches to registries of databases and services for the biomedical sciences

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    The recent explosion of biological data and the concomitant proliferation of distributed databases make it challenging for biologists and bioinformaticians to discover the best data resources for their needs, and the most efficient way to access and use them. Despite a rapid acceleration in uptake of syntactic and semantic standards for interoperability, it is still difficult for users to find which databases support the standards and interfaces that they need. To solve these problems, several groups are developing registries of databases that capture key metadata describing the biological scope, utility, accessibility, ease-of-use and existence of web services allowing interoperability between resources. Here, we describe some of these initiatives including a novel formalism, the Database Description Framework, for describing database operations and functionality and encouraging good database practise. We expect such approaches will result in improved discovery, uptake and utilization of data resources. Database URL: http://www.casimir.org.uk/casimir_dd

    Protein coalitions in a core mammalian biochemical network linked by rapidly evolving proteins

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    <p>Abstract</p> <p>Background</p> <p>Cellular ATP levels are generated by glucose-stimulated mitochondrial metabolism and determine metabolic responses, such as glucose-stimulated insulin secretion (GSIS) from the ÎČ-cells of pancreatic islets. We describe an analysis of the evolutionary processes affecting the core enzymes involved in glucose-stimulated insulin secretion in mammals. The proteins involved in this system belong to ancient enzymatic pathways: glycolysis, the TCA cycle and oxidative phosphorylation.</p> <p>Results</p> <p>We identify two sets of proteins, or protein coalitions, in this group of 77 enzymes with distinct evolutionary patterns. Members of the glycolysis, TCA cycle, metabolite transport, pyruvate and NADH shuttles have low rates of protein sequence evolution, as inferred from a human-mouse comparison, and relatively high rates of evolutionary gene duplication. Respiratory chain and glutathione pathway proteins evolve faster, exhibiting lower rates of gene duplication. A small number of proteins in the system evolve significantly faster than co-pathway members and may serve as rapidly evolving adapters, linking groups of co-evolving genes.</p> <p>Conclusions</p> <p>Our results provide insights into the evolution of the involved proteins. We find evidence for two coalitions of proteins and the role of co-adaptation in protein evolution is identified and could be used in future research within a functional context.</p

    Characterization of functional methylomes by next-generation capture sequencing identifies novel disease-associated variants.

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    Most genome-wide methylation studies (EWAS) of multifactorial disease traits use targeted arrays or enrichment methodologies preferentially covering CpG-dense regions, to characterize sufficiently large samples. To overcome this limitation, we present here a new customizable, cost-effective approach, methylC-capture sequencing (MCC-Seq), for sequencing functional methylomes, while simultaneously providing genetic variation information. To illustrate MCC-Seq, we use whole-genome bisulfite sequencing on adipose tissue (AT) samples and public databases to design AT-specific panels. We establish its efficiency for high-density interrogation of methylome variability by systematic comparisons with other approaches and demonstrate its applicability by identifying novel methylation variation within enhancers strongly correlated to plasma triglyceride and HDL-cholesterol, including at CD36. Our more comprehensive AT panel assesses tissue methylation and genotypes in parallel at ∌4 and ∌3 M sites, respectively. Our study demonstrates that MCC-Seq provides comparable accuracy to alternative approaches but enables more efficient cataloguing of functional and disease-relevant epigenetic and genetic variants for large-scale EWAS.This work was supported by a Canadian Institute of Health Research (CIHR) team grant awarded to E.G., A.T., M.C.V. and M.L. (TEC-128093) and the CIHR funded Epigeneome Mapping Centre at McGill University (EP1-120608) awarded to T.P. and M.L., and the Swedish Research Council, Knut and Alice Wallenberg Foundation and the Torsten Söderberg Foundation awarded to L.R. F.A. holds studentship from The Research Institute of the McGill University Health Center (MUHC). F.G. is a recipient of a research fellowship award from the Heart and Stroke Foundation of Canada. A.T. is the director of a Research Chair in Bariatric and Metabolic Surgery. M.C.V. is the recipient of the Canada Research Chair in Genomics Applied to Nutrition and Health (Tier 1). E.G. and T.P. are recipients of a Canada Research Chair Tier 2 award. The MuTHER Study was funded by a programme grant from the Wellcome Trust (081917/Z/07/Z) and core funding for the Wellcome Trust Centre for Human Genetics (090532). TwinsUK was funded by the Wellcome Trust; European Community's Seventh Framework Programme (FP7/2007-2013). The study also receives support from the National Institute for Health Research (NIHR)-funded BioResource, Clinical Research Facility and Biomedical Research Centre based at Guy's and St Thomas' NHS Foundation Trust in partnership with King's College London. T.D.S. is a holder of an ERC Advanced Principal Investigator award. SNP genotyping was performed by The Wellcome Trust Sanger Institute and National Eye Institute via NIH/CIDR. Finally, we thank the NIH Roadmap Epigenomics Consortium and the Mapping Centers (http://nihroadmap.nih.gov/epigenomics/) for the production of publicly available reference epigenomes. Specifically, we thank the mapping centre at MGH/BROAD for generation of human adipose reference epigenomes used in this study.This is the final version. It was first published by NPG at http://www.nature.com/ncomms/2015/150529/ncomms8211/full/ncomms8211.html#abstrac

    A Systems Model for Immune Cell Interactions Unravels the Mechanism of Inflammation in Human Skin

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    Inflammation is characterized by altered cytokine levels produced by cell populations in a highly interdependent manner. To elucidate the mechanism of an inflammatory reaction, we have developed a mathematical model for immune cell interactions via the specific, dose-dependent cytokine production rates of cell populations. The model describes the criteria required for normal and pathological immune system responses and suggests that alterations in the cytokine production rates can lead to various stable levels which manifest themselves in different disease phenotypes. The model predicts that pairs of interacting immune cell populations can maintain homeostatic and elevated extracellular cytokine concentration levels, enabling them to operate as an immune system switch. The concept described here is developed in the context of psoriasis, an immune-mediated disease, but it can also offer mechanistic insights into other inflammatory pathologies as it explains how interactions between immune cell populations can lead to disease phenotypes

    Meta-analysis of type 2 Diabetes in African Americans Consortium

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    Type 2 diabetes (T2D) is more prevalent in African Americans than in Europeans. However, little is known about the genetic risk in African Americans despite the recent identification of more than 70 T2D loci primarily by genome-wide association studies (GWAS) in individuals of European ancestry. In order to investigate the genetic architecture of T2D in African Americans, the MEta-analysis of type 2 DIabetes in African Americans (MEDIA) Consortium examined 17 GWAS on T2D comprising 8,284 cases and 15,543 controls in African Americans in stage 1 analysis. Single nucleotide polymorphisms (SNPs) association analysis was conducted in each study under the additive model after adjustment for age, sex, study site, and principal components. Meta-analysis of approximately 2.6 million genotyped and imputed SNPs in all studies was conducted using an inverse variance-weighted fixed effect model. Replications were performed to follow up 21 loci in up to 6,061 cases and 5,483 controls in African Americans, and 8,130 cases and 38,987 controls of European ancestry. We identified three known loci (TCF7L2, HMGA2 and KCNQ1) and two novel loci (HLA-B and INS-IGF2) at genome-wide significance (4.15 × 10(-94)<P<5 × 10(-8), odds ratio (OR)  = 1.09 to 1.36). Fine-mapping revealed that 88 of 158 previously identified T2D or glucose homeostasis loci demonstrated nominal to highly significant association (2.2 × 10(-23) < locus-wide P<0.05). These novel and previously identified loci yielded a sibling relative risk of 1.19, explaining 17.5% of the phenotypic variance of T2D on the liability scale in African Americans. Overall, this study identified two novel susceptibility loci for T2D in African Americans. A substantial number of previously reported loci are transferable to African Americans after accounting for linkage disequilibrium, enabling fine mapping of causal variants in trans-ethnic meta-analysis studies.Peer reviewe
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