Biogenesis of protein bodies during vicilin accumulation in Medicago truncatula immature seeds

BACKGROUND: Grain legumes play a worldwide role as a source of plant proteins for feed and food. In the model legume Medicago truncatula, the organisation of protein storage vacuoles (PSV) in maturing seeds remains unknown. FINDINGS: The sub-cellular events accompanying the accumulation of vicilin (globulin7S) were analysed during seed mid-maturation. Immuno-detection of vicilin in light microscopy, allowed a semi-quantitative assessment of the protein body complement. The identified populations of vicilin-containing protein bodies are distinguished by their number and size which allowed to propose a model of their biogenesis. Two distributions were detected, enabling a separation of their processing at early and mid maturation stages. The largest protein bodies, at 16 and 20 days after pollination (DAP), were formed by the fusion of small bodies. They have probably attained their final size and correspond to mature vicilin aggregations. Electron microscopic observations revealed the association of the dense protein bodies with rough endoplasmic reticulum. The presence of a ribosome layer surrounding protein bodies, would support an endoplasmic reticulum–vacuole trafficking pathway. CONCLUSIONS: The stastistic analysis may be useful for screening mutations of candidate genes governing protein content. The definitive evidence for an ER-storage vacuole pathway corresponds to a challenge, for the storage of post-translationally unstable proteins. It was proposed for the accumulation of one class of storage protein, the vicilins. This alternative pathway is a matter of controversy in dicotyledonous seeds

Simulation of distillation process with packings and trays in order to optimize the dimensions

Orientador: Maria Regina Wolf MacielDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia QuímicaResumo: Nesta dissertação serão apresentados resultados de simulações de uma coluna de destilação de bioetanol com tecnologias de recheios e pratos visando obter uma configuração otimizada que possa reduzir as dimensões das colunas, tanto na altura quanto no diâmetro. Uma análise técnica e comparativa das tecnologias de pratos calotados, pratos valvulados, recheios estruturados e recheios randômicos serão apresentadas neste trabalho, bem como a comparação com a configuração de uma coluna de destilação convencional de pratos calotados com produção de bioetanol de 600 m3/d. As colunas B e B1 de destilarias convencionais serão consideradasAbstract: In this work, many simulations results of bioethanol distillation columns with packings and trays technologies are presented in order to obtain an optimal configuration that can reduce the size of the columns, both in height and in diameter. A technical and comparative analysis of bubble cap trays, valve trays, structured packing and random packing will be presented, as well the comparison with the configuration of a conventional distillation column of bubble cap trays with bioethanol production of 600 m3/d. The columns B and B1 of conventional distilleries will be consideredMestradoDesenvolvimento de Processos QuímicosMestre em Engenharia Químic

A representatividade negra no mundo dos heróis

Este trabalho trata da representatividade da população negra no universo dos heróis, considerando quadrinhos, filmes e franquias de super-heróis. A pesquisa considera aspectos históricos e sociais das populações negras escravizadas no Brasil e nos Estados Unidos da América, que influenciaram na percepção destas populações frente a uma cultura opressiva dominante. A pesquisa foi centrada em obras com personagens e protagonistas negros, para a construção de personagens cuja figura relacional esteja inserida dentro do campo do heroico. Foi feita uma análise de obras históricas, uma pesquisa sobre os elementos necessários à construção da identidade negra e suas expressões de representatividade, com uma análise dos primeiros personagens e dos principais super-heróis, sua construção frente à realidade das sociedades onde estão inseridos e às influências que sofreram, dentro de aspectos de identidade, representatividade, direitos fundamentais, preconceito racial e racismo estrutural. Também é objeto da pesquisa os conceitos de whitewashing, castas sociais, color-blind casting, interseccionalidade, blackface, alteridade, representatividade tóxica e afrofuturismo para o cenário de representatividade negra no mundo dos heróis, a partir de alguns exemplos selecionados

O direito ao esquecimento como limite à superexposição de crianças e adolescentes

O presente trabalho tem por objetivo ensejar uma readaptação do entendimento firmado pelo Supremo Tribunal Federal acerca do Direito ao esquecimento, não contemplado em julgamento de recurso com repercussão geral no Tema 786. Em um primeiro momento, são expostas as inspirações e os princípios basilares que fundamentam a importância desse reconhecimento em prol do direito à dignidade da pessoa humana, a partir da qual se irradiam todos os demais direitos fundamentais. A segunda parte a ser analisada trata-se da superexposição de crianças e adolescentes, em uma rede de compartilhamento elevado de informações e, por conseguinte, os reflexos ao longo da vida desses grupo. Apesar da inexistência de legislação específica que verse sobre a temática, será observada a possibilidade de proteção do instituto do esquecimento, a qual afronta dispositivos constitucionais e infralegais já expressamente reconhecidos pelo legislador. Por fim, será verificada a possibilidade de novos elementos normativos que se adequem mais ao ordenamento jurídico brasileiro em um cenário de armazenamento de dados e de desenvolvimento psicossocial

Un jeu de ping-pong entre la France et le Liban

À l’occasion de l’exposition Albums, le Musée de l’histoire de l’immigration a acquis des planches de Zeina Abirached, née à Beyrouth en 1981 et qui vit en France depuis 2004. A travers ses bandes dessinées, elle interroge ses liens avec son pays d’origine, son bilinguisme, sa double culture en tant que femme enracinée et nomade à la fois

Un jeu de ping-pong entre la France et le Liban

À l’occasion de l’exposition Albums, le Musée de l’histoire de l’immigration a acquis des planches de Zeina Abirached, née à Beyrouth en 1981 et qui vit en France depuis 2004. A travers ses bandes dessinées, elle interroge ses liens avec son pays d’origine, son bilinguisme, sa double culture en tant que femme enracinée et nomade à la fois

Effect of Acid Treatment on Interfacial and Foam Properties of Soy Proteins

The goal of the present work was to study the effects of acid treatment on the foaming properties of a soybean protein isolate (SPI) and its fractions, glycinin (11S) and β-conglycinin (7S). The structural characteristics, interfacial properties, foaming capacity and stability of the treated proteins were studied. Results from surface hydrophobicity and differential scanning calorimetry (DSC) showed that the acid treatment caused the complete denaturation of 11S and a partial denaturation of 7S. This protein unfolding affected their interfacial properties, which led to an improvement in the foaming properties of both protein fractions and isolate. Treated 7S showed the best behavior in the rearrangement process, probably due to its smaller size and its modified structural characteristics. All treated proteins showed stronger interfacial films. The foams of treated proteins were destabilized mostly due to gravitational drainage rather than Ostwald ripening.Centro de Investigación y Desarrollo en Criotecnología de AlimentosConsejo Nacional de Investigaciones Científicas y Técnica

Effect of Acid Treatment on Interfacial and Foam Properties of Soy Proteins

The goal of the present work was to study the effects of acid treatment on the foaming properties of a soybean protein isolate (SPI) and its fractions, glycinin (11S) and β-conglycinin (7S). The structural characteristics, interfacial properties, foaming capacity and stability of the treated proteins were studied. Results from surface hydrophobicity and differential scanning calorimetry (DSC) showed that the acid treatment caused the complete denaturation of 11S and a partial denaturation of 7S. This protein unfolding affected their interfacial properties, which led to an improvement in the foaming properties of both protein fractions and isolate. Treated 7S showed the best behavior in the rearrangement process, probably due to its smaller size and its modified structural characteristics. All treated proteins showed stronger interfacial films. The foams of treated proteins were destabilized mostly due to gravitational drainage rather than Ostwald ripening.Centro de Investigación y Desarrollo en Criotecnología de AlimentosConsejo Nacional de Investigaciones Científicas y Técnica

The role of the pod in seed development: strategies for manipulating yield

Pods play a key role in encapsulating the developing seeds and protecting them from pests and pathogens. In addition to this protective function, it has been shown that the photosynthetically active pod wall contributes assimilates and nutrients to fuel seed growth. Recent work has revealed that signals originating from the pod may also act to coordinate grain filling and regulate the reallocation of reserves from damaged seeds to those that have retained viability. In this review we consider the evidence that pods can regulate seed growth and maturation, particularly in members of the Brassicaceae family, and explore how the timing and duration of pod development might be manipulated to enhance either the quantity of crop yield or its nutritional properties