Number needed to freeze: cumulative live birth rate after fertility preservation in women with endometriosis

Research question: How does the number of oocytes used affect the cumulative live birth rate in endometriosis patients who had their oocytes vitrified for fertility preservation (FP)? Design: Retrospective observational study including data from 485 women with endometriosis who underwent FP from January 2007 to July 2018. Survival curves and Kaplan-Meier plots were used to analyse the cumulative live birth rate (CLBR) according to the number of vitrified oocytes used. Data were stratified according to age, stage of the disease and ovarian surgery prior to FP (operated vs. non-operated). Endometriosis curves were compared to plots developed using elective fertility preservation (EFP) patients as control group. Log-rank, Breslow and Tarone-Ware tests were used to compare the survival curves. Results: The CLBR increased as the number of oocytes used per patient rose, reaching 89.5% (95% CI=80.0-99.1) using 22 oocytes. Higher outcomes were observed in young women (≤35 y. vs. >35 y). In the younger group, the CLBR was 95.4% (95% CI=87.2-103.6) using ~20 oocytes vs. 79.6% (95% CI=58.1-101.1) in older women (P<0.05). No statistical differences were observed in overall calculations and according to age when the CLBR was compared between operated and non-operated women (NS). Comparable outcomes were also observed in stages I-II vs. III-IV (NS). The mean age was higher in EFP patients (37.2 ± 4.9 vs. 35.7 ± 3.7; P<0.05). The outcome was better in the endometriosis group as compared to EFP (P<0.05): a CLBR of 89.5% (80.0-99.1) vs. 59.9% (51.4-68.6) when 22 oocytes were used (P<0.05). However, the difference was milder when fewer oocytes were used in both groups. When comparisons were made between age-matching groups, no statistical differences were observed (NS). Conclusion: The probability of live birth increases as the number of oocytes used rises in patients with endometriosis, but better outcomes were observed among young women. Neither the stage of the disease nor prior surgical excision of ovarian endometrioma were related to success. No statistical differences in age matching groups were observed when comparing to EFP patients. The information provided herein may be of interest to both patients and treating physicians for counselling purposes

Neutron calibrations in dark matter searches: the ANAIS-112 case

ANAIS is a direct dark matter detection experiment whose goal is to confirm or refute in a model independent way the positive annual modulation signal claimed by DAMA/LIBRA. Consisting of 112.5 kg of NaI(Tl) scintillators, ANAIS-112 is taking data at the Canfranc Underground Laboratory in Spain since August, 2017. Results corresponding to the analysis of three years of data are compatible with the absence of modulation and incompatible with DAMA/LIBRA. However, testing this signal relies on the knowledge of the scintillation quenching factors (QF), which measure the relative efficiency for the conversion into light of the nuclear recoil energy with respect to the same energy deposited by electrons. Previous measurements of the QF in NaI(Tl) show a large dispersion. Consequently, in order to better understand the response of the ANAIS-112 detectors to nuclear recoils, a specific neutron calibration program has been developed. This program combines two different approaches: on the one hand, QF measurements were carried out in a monoenergetic neutron beam; on the other hand, the study presented here aims at the evaluation of the QF by exposing directly the ANAIS-112 crystals to neutrons from low activity $^{252}$Cf sources, placed outside the lead shielding. Comparison between these onsite neutron measurements and detailed GEANT4 simulations will be presented, confirming that this approach allows testing different QF models.Comment: Contributed to the TAUP2023 Conference, August-September 2023. To be published in Proceeding of Scienc

Spanish ATLAS tier-2: Facing up to LHC Run 2

The goal of this work is to describe the way of addressing the main challenges of Run 2 by the Spanish ATLAS Tier-2. The considerable increase of energy and luminosity for the upcoming Run 2 with respect to Run 1 has led to a revision of the ATLAS computing model as well as some of the main ATLAS computing tools. In this paper, the adaptation to these changes will be described. The Spanish ATLAS Tier-2 is a R&D project which consists of a distributed infrastructure composed of three sites and its members are involved in ATLAS computing progress, namely the work in different tasks and the development of new tools (e.g. Event Index)This work has been supported by MINECO, Spain (Proj. Ref. FPA2010-21919-C03-01,02,03 & FPA2013-47424-C3,01,02,03), which include FEDER funds from the European Unio

Angle-resolved photoemission study and first principles calculation of the electronic structure of GaTe

The electronic band structure of GaTe has been calculated by numerical atomic orbitals density-functional theory, in the local density approximation. In addition, the valence-band dispersion along various directions of the GaTe Brillouin zone has been determined experimentally by angle-resolved photoelectron spectroscopy. Along these directions, the calculated valence-band structure is in good concordance with the valence-band dispersion obtained by these measurements. It has been established that GaTe is a direct-gap semiconductor with the band gap located at the Z point, that is, at Brillouin zone border in the direction perpendicular to the layers. The valence-band maximum shows a marked \textit{p}-like behavior, with a pronounced anion contribution. The conduction band minimum arises from states with a comparable \textit{s}- \textit{p}-cation and \textit{p}-anion orbital contribution. Spin-orbit interaction appears to specially alter dispersion and binding energy of states of the topmost valence bands lying at $\Gamma$. By spin-orbit, it is favored hybridization of the topmost \textit{p}$_z$-valence band with deeper and flatter \textit{p$_x$}-\textit{p$_y$} bands and the valence-band minimum at $\Gamma$ is raised towards the Fermi level since it appears to be determined by the shifted up \textit{p$_x$}-\textit{p$_y$} bands.Comment: 7 text pages, 6 eps figures, submitted to PR

Portable magnetic resonance imaging of patients indoors, outdoors and at home

Mobile medical imaging devices are invaluable for clinical diagnostic purposes both in and outside healthcare institutions. Among the various imaging modalities, only a few are readily portable. Magnetic resonance imaging (MRI), the gold standard for numerous healthcare conditions, does not traditionally belong to this group. Recently, low-field MRI start-up companies have demonstrated the first decisive steps towards portability within medical facilities, but these are so far incompatible with more demanding use cases such as in remote and developing regions, sports facilities and events, medical and military camps, or home healthcare. Here we present in vivo images taken with a light, home-made, low-field extremity MRI scanner outside the controlled environment provided by medical facilities. To demonstrate the true portability of the system and benchmark its performance in various relevant scenarios, we have acquired images of a volunteer's knee in: i) an MRI physics laboratory; ii) an office room; iii) outside a campus building, connected to a nearby power outlet; iv) in open air, powered from a small fuel-based generator; and v) at the volunteer's home. All images have been acquired within clinically viable times, and signal-to-noise ratios (SNR) and tissue contrast suffice for 2D and 3D reconstructions with diagnostic value, with comparable overall image quality across all five situations. Furthermore, the volunteer carries a fixation metallic implant screwed to the femur, which leads to strong artifacts in standard clinical systems but appears sharp in our low-field acquisitions. Altogether, this work opens a path towards highly accessible MRI under circumstances previously unrealistic.Comment: 17 pages, 4 figures, comments welcom

Cell identity and nucleo-mitochondrial genetic context modulate OXPHOS performance and determine somatic heteroplasmy dynamics

Heteroplasmy, multiple variants of mitochondrial DNA (mtDNA) in the same cytoplasm, may be naturally generated by mutations but is counteracted by a genetic mtDNA bottleneck during oocyte development. Engineered heteroplasmic mice with nonpathological mtDNA variants reveal a nonrandom tissue-specific mtDNA segregation pattern, with few tissues that do not show segregation. The driving force for this dynamic complex pattern has remained unexplained for decades, challenging our understanding of this fundamental biological problem and hindering clinical planning for inherited diseases. Here, we demonstrate that the nonrandom mtDNA segregation is an intracellular process based on organelle selection. This cell type-specific decision arises jointly from the impact of mtDNA haplotypes on the oxidative phosphorylation (OXPHOS) system and the cell metabolic requirements and is strongly sensitive to the nuclear context and to environmental cues

Antinociceptive and antiedema effects produced in rats by Brassica oleracea var. italica sprouts involving sulforaphane

Natural products are recognized as potential analgesics since many of them are part of modern medicine to relieve pain without serious adverse effects. The aim of this study was to investigate the antinociceptive and anti-inflammatory activities of an aqueous extract of Brassica oleracea var. italica sprouts (AEBS) and one of its main reported bioactive metabolites sulforaphane (SFN). Antinociceptive activity of the AEBS (30, 100, and 300 mg/kg, i.p. or 1000 and 2000 mg/kg, p.o.) and SFN (0.1 mg/kg, i.p.) was evaluated in the plantar test in rats to reinforce its analgesic-like activity at central level using the reference drug tramadol (TR, 50 mg/kg, i.p.). The anti-inflammatory-like response was determined in the carrageenan-induced oedema at the same dosages for comparison with ketorolac (KET, 20 mg/kg, i.p.) or indomethacin (INDO, 20 mg/kg, p.o.). A histological analysis of the swollen paw was included to complement the anti-inflammatory response. Additionally, acute toxicity observed in clinical analgesics as the most common adverse effects, such as sedation and/or gastric damage, was also explored. As a result, central and peripheral action of the AEBS was confirmed using enteral and parenteral administration, in which significant reduction of the nociceptive and inflammatory responses resembled the effects of TR, KET, or INDO, respectively, involving the presence of SFN. No adverse or toxic effects were observed in the presence of the AEBS or SFN. In conclusion, this study supports that Brassica oleracea var. italica sprouts are a potential source of antinociceptive natural products such as SFN for therapy of pain alone and associated to an inflammation conditionOpen Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This work was partially supported by the projects CONACYT-22645419 and 256448, and the "Fundación Séneca"—Murcia Regional Agency for Science and Technology, Project 20855/PI/18. NB is granted by a “Juan de la Cierva Incorporación” (IJC-2020-044496-I) post-doctoral contract funded by MCIN/AEI/1013039/501100011033 and European Union NextGenerationEU/PRTR. Authors would also like to thank the CYTED Programme, Action 112RT0460 CORNUCOPIA Thematic NetworkPeer reviewe

"Invisible burials" and fragmentation practices in Iron Age Europe:Excavations at the Monte Bernorio Necropolis (Northern Spain)

The scarcity of burial remains in large parts of Iron Age Europe, particularly in the Atlantic regions, has often led scholars to discuss the apparent “invisibility” of graves. This paper presents the results from several excavation campaigns at Monte Bernorio, one of the most important sites of the 1st millennium b.c. on the Iberian Peninsula. The fieldwork and post-excavation work carried out in the area of the necropolis have identified numerous burial pits, with complex ritual activities characterized by fragmentation and the practice of the pars pro toto. In addition, evidence for later rituals in some of the graves can be linked to ancestor worship. The results provide important insights into funerary practices in Late Iron Age Europe, leading us to rethink the very meaning of cemeteries in the study area and beyond.- Burial Traditions in Iron Age Europe - The Monte Bernorio Archaeological Zone - The 2007–2008 Necropolis Excavations - The 2015–2016 Necropolis Excavations - Post-Excavation Work and Interpretation: The Faunal and Human Remains - Structure and Chronology of Monte Bernorio Area 7 - Destruction of the Body, Commemoration in the Absence of a Corpse, and Visibility of the Mortuary Rite

Controlled 3D-coating of the pores of highly ordered mesoporous antiferromagnetic Co3O4 replicas with ferrimagnetic FexCo3-xO4 nanolayers

The controlled filling of the pores of highly ordered mesoporous antiferromagnetic Co3O4 replicas with ferrimagnetic FexCo3-xO4 nanolayers is presented as a proof-of-concept toward the integration of nanosized units in highly ordered, heterostructured 3D architectures. Antiferromagnetic (AFM) Co3O 4 mesostructures are obtained as negative replicas of KIT-6 silica templates, which are subsequently coated with ferrimagnetic (FiM) Fe xCo3-xO4 nanolayers. The tuneable magnetic properties, with a large exchange bias and coercivity, arising from the FiM/AFM interface coupling, confirm the microstructure of this novel two-phase core-shell mesoporous material. The present work demonstrates that ordered functional mesoporous 3D-materials can be successfully infiltrated with other compounds exhibiting additional functionalities yielding highly tuneable, versatile, non-siliceous based nanocomposites

Correspondence: Strongly-driven Re + CO2 redox reaction at high-pressure and high-temperature

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