183 research outputs found
Microbiome dynamics during the HI-SEAS IV mission, and implications for future crewed missions beyond Earth.
Background: Human health is closely interconnected with its microbiome. Resilient microbiomes in, on, and around the human body will be key for safe and successful long-term space travel. However, longitudinal dynamics of microbiomes inside confined built environments are still poorly understood. Herein, we used the Hawaii Space Exploration Analog and Simulation IV (HI-SEAS IV) mission, a 1 year-long isolation study, to investigate microbial transfer between crew and habitat, in order to understand adverse developments which may occur in a future outpost on the Moon or Mars.
Results: Longitudinal 16S rRNA gene profiles, as well as quantitative observations, revealed significant differences in microbial diversity, abundance, and composition between samples of the built environment and its crew. The microbiome composition and diversity associated with abiotic surfaces was found to be rather stable, whereas the microbial skin profiles of individual crew members were highly dynamic, resulting in an increased microbiome diversity at the end of the isolation period. The skin microbiome dynamics were especially pronounced by a regular transfer of the indicator species Methanobrevibacter between crew members within the first 200 days. Quantitative information was used to track the propagation of antimicrobial resistance in the habitat. Together with functional and phenotypic predictions, quantitative and qualitative data supported the observation of a delayed longitudinal microbial homogenization between crew and habitat surfaces which was mainly caused by a malfunctioning sanitary facility.
Conclusions: This study highlights main routes of microbial transfer, interaction of the crew, and origins of microbial dynamics in an isolated environment. We identify key targets of microbial monitoring, and emphasize the need for defined baselines of microbiome diversity and abundance on surfaces and crew skin. Targeted manipulation to counteract adverse developments of the microbiome could be a highly important strategy to ensure safety during future space endeavors
The body composition monitor: a flexible tool for routine fluid management across the haemodialysis population
Bioimpedance measurements with the Body Composition Monitor (BCM) have been shown to improve fluid management in haemodialysis. However, there is a lack of a sufficiently robust evidence-base for use of the BCM outside of standard protocols. This study aims to define the error associated with BCM measurement using alternate paths and timings to allow the use of BCM with confidence in a range of clinical scenarios. BCM measurements were made in 48 healthy controls and in 48 stable haemodialysis patients before and immediately after dialysis. The effect of utilising alternative measurement paths was assessed using mixed effects models and the effect of measuring post-dialysis was assessed by comparing changes in BCM-measured overhydration (OH) with weight changes over dialysis. The data from healthy controls suggest that there is no difference in BCM-measured OH between all the whole-body paths other than the ankle-to-ankle measurement. Dialysis patients showed similar results other than having higher BCM-measured OH when measured across the site of a vascular access. There was good agreement between BCM-measured OH from the standard path and change in weight, suggesting post-dialysis measurements can be utilised. These results suggest BCM protocols can be flexible regarding measurement paths and timing of measurement to ensure as many patients as possible can benefit from the technology
Experimental validation of a method for removing the capacitive leakage artifact from electrical bioimpedance spectroscopy measurements
Significance Tests for Periodogram Peaks
We discuss methods currently in use for determining the significance of peaks
in the periodograms of time series. We discuss some general methods for
constructing significance tests, false alarm probability functions, and the
role played in these by independent random variables and by empirical and
theoretical cumulative distribution functions. We also discuss the concept of
"independent frequencies" in periodogram analysis. We propose a practical
method for estimating the significance of periodogram peaks, applicable to all
time series irrespective of the spacing of the data. This method, based on
Monte Carlo simulations, produces significance tests that are tailor-made for
any given astronomical time series.Comment: 22 pages, 11 Encapsulated Postscript figures, AAS LaTeX v5.2
Submitted to Ap
Analysis of bacterial profiles of AGBRESA participants – a study concerning terrestrial astronauts under simulated microgravity
Introduction:
Long-term space missions are accompanied by harmful environmental conditions like
microgravity. Due to the reduced gravity, astronauts adapt to their environment resulting in
tissue fluidic shifts. Since the knowledge about microbiome data in space is sparse and
conduction of experiments at the ISS is complex, suitable analogs are needed. Therefore,
the first cooperative bed-rest study called Artificial Gravity Bed-Rest study with ESA
(AGBRESA), by NASA, ESA and DLR offered optimal features to investigate possible
correlations between microbial shifts and physiological microgravity by using -6° head-downtilt (HDT). The aim of this survey was to identify changes within the standardized conditions,
such as diet and wrongly distributed tissue fluids to reveal causal connections among health
state and microbial communities
Resonant Cavity Perturbation: A Promising New Method for the Assessment of Total Body Water in Children
The accurate measurement of total body water (TBW) in children has important clinical and nutritional applications. Resonant cavity perturbation (RCP) is a new method for estimating TBW. This method measures the dielectric properties of the body which are related to body water. For RCP measurements, each subject lay supine on a bed inside a screened room which acts as a resonant cavity. A network analyser measures the frequencies of two low-order cavity resonances of the room, with electric-field vectors that were respectively vertical and horizontal, the resonant frequency shifts relative to the empty room are then derived. These frequency shifts correlates with TBW. The aims of this present study were to a) develop TBWRCP predictive equations for children using TBWdil as the criterion method, b) cross-validate the derived equations, c) determine precision of the TBWRCP method, and d) compare the criterion method TBWdil with three methods of estimating TBW: RCP, MFBIS and Anthropometry. Predictive equations, independent of sex, were developed with linear regression in a group of 36 children. The relationship between combined RCP frequency shifts and TBWdilution had an r2=0.90 and standard error of the estimate (SEE) =1.42kg. Multiple regression analysis, that included a term for body mass index, only had a small effect on r2=0.93 and SEE=1.25kg. In-vivo TBW precision for the vertical, horizontal and combined frequency modes ranged from 0.7 to 3.4%. Bland Altman analysis indicated close agreement between the criterion method TBWdil and the three other methods of TBW estimation. Mean differences were TBWRCP(2) = 0.01+/-1.34kg, TBWMFBIS = 0.45+/-1.35kg, TBWAnthropometry = 0.29+/-1.29kg. Currently the RCP method does not significantly improve the prediction of TBW compared to MFBIS and anthropometry in this initial study. However the derived equation was independent of sex and body size had only a small effect
Microbial Metabolism of Amino Acids—Biologically Induced Removal of Glycine and the Resulting Fingerprint as a Potential Biosignature
The identification of reliable biomarkers, such as amino acids, is key for the search of extraterrestrial life. A large number of microorganisms metabolize, synthesize, take up and excrete amino acids as part of the amino acid metabolism during aerobic and/or anaerobic respiration or in fermentation. In this work, we investigated whether the anaerobic microbial metabolism of amino acids could leave a secondary biosignature indicating biological activity in the environment around the cells. The observed fingerprints would reflect the physiological capabilities of the specific microbial community under investigation. The
metabolic processing of an amino acid mixture by two distinct anaerobic microbial communities collected from Islinger Mühlbach (ISM) and Sippenauer Moor (SM), Germany was examined. The amino acid mixture contained L-alanine, β-alanine, L-aspartic acid, DL-proline, L-leucine, L-valine, glycine, L-phenylalanine and L-isoleucine. In parallel, an amino acid spiked medium without microorganisms was used as a control to determine abiotic changes over time. Liquid chromatography mass spectrometry (LC-MS) was used to track amino acid changes over time. When comparing to the control samples that did not show significant changes of amino acids concentrations over time, we found that glycine was almost completely depleted from both microbial samples to less than 3% after the first two weeks- This results indicates a preferential use of this simple amino acid by these microbial communities. Although glycine
degradation can be caused by abiotic processes, these results show that its preferential depletion in an environment would be consistent with the presence of life. We found changes in most other amino acids that varied between amino acids and communities, suggesting complex dynamics with no clear universal pattern that might be used as a signature of life. However, marked increases in amino acids, caused by cellular synthesis and release into the extracellular environment (e.g., alanine), were observed and could be considered a signature of metabolic activity. We conclude, that substantial anomalous enhancements of some amino acids against the expected abiotic background concentration may be an agnostic signature of the presence of biological processes
MICROORGANISMS FROM MARS ANALOGUE ENVIRONMENTS IN EARTH - COULD THEY SURVIVE ON MARS?
Assessing the habitability of Mars and detecting life, if it was ever there, depends on knowledge
of whether the combined environmental stresses experienced on Mars are compatible with life
and whether a record of that life could ever be detected. Many combinations of Mars relevant
stress factors, such as high radiation dose rates and high UV
uences combined with high salt
concentrations, and low water activity, have not been investigated. In particular, the response
of anaerobic organisms to Mars-like stress factors and combinations thereof are not known. In
the EC project MASE (Mars Analogues for Space Exploration) we address these limitations by
characterising different Mars analogue environments on Earth, isolating microorganisms from
these sites and exposing them to Mars relevant stress factors alone and in combination. We
want to find out, if these bacteria respond in an additive or synergistic way and if they would
be able to survive on Mars. So far, eight only distantly related microorganisms are under
detailed investigation, e.g Yersinia sp., Halanaerobium sp., Acidiphilum sp. Desulfovibrio sp..
Unexpectedly, a Yersinia strain turned out to be quite resistant, especially against desicca-
tion and oxidising compounds, whereas a Desulfovibrio sp. strain exhibit a relatively high
radiation resistance. The future experiments aim at the identification of the underlying cellu-
lar and molecular mechanisms and the comparison to other new isolates from Mars analogue
environments on Earth in the MASE project
BIOMARKERS DETECTION IN MARS ANALOGUE SITES WITHIN MASE PROJECT
Life is a physico-chemical process by which tell-tale signals or traces are left on the environment. These signals are indicators of life and are known as biomarkers.
Besides, the traces of some kinds of microorganisms can be well preserved, provided that they are rapidly mineralized and that the sediments in which they occur are rapidly cemented [1].
The search for these traces of life is one of the main objectives of Mars exploration [1] and to improve and optimize the search and detection of them forms part of MASE project targets.
In MASE project (Mars Analogues for Space Exploration) we work to improve approaches and methods for biomarker detection in samples with low biomass from Mars analogue sites.
A developed antibody multiarray competitive immunoassay (MACIA) for the simultaneous detection of compounds of a wide range of molecular sizes or whole spores and cells [2] [3] has revealed as suitable option to achieve this purpose
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