45 research outputs found
Pt(II) coordination complexes as visible light photocatalysts for the oxidation of sulfides using batch and flow processes
A new catalytic system for the photooxidation of sulfides based on Pt(ii) complexes is presented. The catalyst is capable of oxidizing a large number of sulfides containing aryl, alkyl, allyl, benzyl, as well as more complex structures such as heterocycles and methionine amino acid, with complete chemoselectivity. In addition, the first sulfur oxidation in a continuous flow process has been developedJ. A. would also like to thank the MICINN for their ‘Ramón y Cajal’ contract and the European Research Council (ERC-CG, contract number 647550
83 Bronchopulmonary infection/colonization in cystic fibrosis: results from a Spanish multicenter study
Development of an epigenetic age predictor for costal cartilage with a simultaneous somatic tissue differentiation system
Age prediction from DNA has been a topic of interest in recent years due to the promising results obtained when using epigenetic markers. Since DNA methylation gradually changes across the individual's lifetime, prediction models have been developed accordingly for age estimation. The tissue-dependence for this biomarker usually necessitates the development of tissue-specific age prediction models, in this way, multiple models for age inference have been constructed for the most commonly encountered forensic tissues (blood, oral mucosa, semen). The analysis of skeletal remains has also been attempted and prediction models for bone have now been reported. Recently, the VISAGE Enhanced Tool was developed for the simultaneous DNA methylation analysis of 8 age-correlated loci using targeted high-throughput sequencing. It has been shown that this method is compatible with epigenetic age estimation models for blood, buccal cells, and bone. Since when dealing with decomposed cadavers or postmortem samples, cartilage samples are also an important biological source, an age prediction model for cartilage has been generated in the present study based on methylation data collected using the VISAGE Enhanced Tool. In this way, we have developed a forensic cartilage age prediction model using a training set composed of 109 samples (19–74 age range) based on DNA methylation levels from three CpGs in FHL2, TRIM59 and KLF14, using multivariate quantile regression which provides a mean absolute error (MAE) of ± 4.41 years. An independent testing set composed of 72 samples (19–75 age range) was also analyzed and provided an MAE of ± 4.26 years. In addition, we demonstrate that the 8 VISAGE markers, comprising EDARADD, TRIM59, ELOVL2, MIR29B2CHG, PDE4C, ASPA, FHL2 and KLF14, can be used as tissue prediction markers which provide reliable blood, buccal cells, bone, and cartilage differentiation using a developed multinomial logistic regression model. A training set composed of 392 samples (n = 87 blood, n = 86 buccal cells, n = 110 bone and n = 109 cartilage) was used for building the model (correct classifications: 98.72%, sensitivity: 0.988, specificity: 0.996) and validation was performed using a testing set composed of 192 samples (n = 38 blood, n = 36 buccal cells, n = 46 bone and n = 72 cartilage) showing similar predictive success to the training set (correct classifications: 97.4%, sensitivity: 0.968, specificity: 0.991). By developing both a new cartilage age model and a tissue differentiation model, our study significantly expands the use of the VISAGE Enhanced Tool while increasing the amount of DNA methylation-based information obtained from a single sample and a single forensic laboratory analysis. Both models have been placed in the open-access Snipper forensic classification website.</p
The de Rham homotopy theory and differential graded category
This paper is a generalization of arXiv:0810.0808. We develop the de Rham
homotopy theory of not necessarily nilpotent spaces, using closed dg-categories
and equivariant dg-algebras. We see these two algebraic objects correspond in a
certain way. We prove an equivalence between the homotopy category of schematic
homotopy types and a homotopy category of closed dg-categories. We give a
description of homotopy invariants of spaces in terms of minimal models. The
minimal model in this context behaves much like the Sullivan's minimal model.
We also provide some examples. We prove an equivalence between fiberwise
rationalizations and closed dg-categories with subsidiary data.Comment: 47 pages. final version. The final publication is available at
http://www.springerlink.co
Long-term affected flat oyster (Ostrea edulis) haemocytes show differential gene expression profiles from naïve oysters in response to Bonamia ostreae
Expressed sequence tags (ESTs) from immune tissues of turbot (Scophthalmus maximus) challenged with pathogens
Background: The turbot (Scophthalmus maximus; Scophthalmidae; Pleuronectiformes) is a flatfish
species of great relevance for marine aquaculture in Europe. In contrast to other cultured flatfish,
very few genomic resources are available in this species. Aeromonas salmonicida and Philasterides
dicentrarchi are two pathogens that affect turbot culture causing serious economic losses to the
turbot industry. Little is known about the molecular mechanisms for disease resistance and hostpathogen interactions in this species. In this work, thousands of ESTs for functional genomic studies
and potential markers linked to ESTs for mapping (microsatellites and single nucleotide
polymorphisms (SNPs)) are provided. This information enabled us to obtain a preliminary view of
regulated genes in response to these pathogens and it constitutes the basis for subsequent and
more accurate microarray analysis.
Results: A total of 12584 cDNAs partially sequenced from three different cDNA libraries of
turbot (Scophthalmus maximus) infected with Aeromonas salmonicida, Philasterides dicentrarchi and
from healthy fish were analyzed. Three immune-relevant tissues (liver, spleen and head kidney)
were sampled at several time points in the infection process for library construction. The
sequences were processed into 9256 high-quality sequences, which constituted the source for the
turbot EST database. Clustering and assembly of these sequences, revealed 3482 different putative
transcripts, 1073 contigs and 2409 singletons. BLAST searches with public databases detected
significant similarity (e-value ≤ 1e-5) in 1766 (50.7%) sequences and 816 of them (23.4%) could be
functionally annotated. Two hundred three of these genes (24.9%), encoding for defence/immunerelated proteins, were mostly identified for the first time in turbot. Some ESTs showed significant differences in the number of transcripts when comparing the three libraries, suggesting regulation
in response to these pathogens. A total of 191 microsatellites, with 104 having sufficient flanking
sequences for primer design, and 1158 putative SNPs were identified from these EST resources in
turbot.
Conclusion: A collection of 9256 high-quality ESTs was generated representing 3482 unique
turbot sequences. A large proportion of defence/immune-related genes were identified, many of
them regulated in response to specific pathogens. Putative microsatellites and SNPs were identified.
These genome resources constitute the basis to develop a microarray for functional genomics
studies and marker validation for genetic linkage and QTL analysis in turbot.This study was supported by a Consellería de Pesca e Asuntos Marítimos and the Dirección Xeral de I+D – Xunta de Galicia project (2004/CP480)S
Synthesis, microstructure and volumetry of novel metal thiocyanate ionic liquids with [BMIM] cation
We present a new family of ionic liquids (ILs) with a common cation, 1-butyl-3-methyl imidazolium, the popular [BMIM]+ (also written C4C1Im+) and a variety of anionic complexes (also called adducts) based in thiocyanate (SCN)?: one blank compound, BMIM(SCN), and ten doped with metals having different oxidation states: Al+3, Mn+2, Fe+3, Cr+3, Ni+2, Hg+2, Zn+2, Co+2, Cd+2 and Cu+, forming, respectively, [BMIM]3[Al(SCN)6], [BMIM]4 Mn(SCN)6, [BMIM]3 Fe(SCN)6, [BMIM]3 Cr(SCN)6, [BMIM]4 Ni(SCN)6, [BMIM]2 Hg(SCN)4, [BMIM]2 Zn(SCN)4, [BMIM]2 Co(SCN)4, [BMIM]2 Cd(SCN)4 and [BMIM]3 Cu(SCN)4. All of them were synthesized by us, except the blank IL and the Co thiocyanate, which are commercial. Obtained products have been characterized by NMR, and also by electrospray ionization, MS-ES, which allows the determination of the new ILs purities. Then, compounds have been analyzed using FT-IR and Raman spectroscopy. In addition, magnetic susceptibility and refractive index measurements were performed in some of the compounds studied, as well as thermal characterization using DSC and TGA. Finally, experimental measurements of density on all those ILs have been performed, and for some of the samples in a broad temperature range (about 100 K). In spite of being very similar compounds from the chemical point of view, they present quite different physical properties, including optical, thermal and magnetic ones? Also, they show different oxidation states (one with +1, six with +2 and other three with +3). We guess that this family of ILs will have interesting applications, mainly for photonic devices
Nucleoside/nucleotide reverse transcriptase inhibitor sparing regimen with once daily integrase inhibitor plus boosted darunavir is non-inferior to standard of care in virologically-suppressed children and adolescents living with HIV – Week 48 results of the randomised SMILE Penta-17-ANRS 152 clinical trial
Whole genome sequencing of turbot (Scophthalmus maximus; Pleuronectiformes):a fish adapted to demersal life
12 páginas, 5 figuras.-- Antonio Figueras ... et al.-- This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly citedThe turbot is a flatfish (Pleuronectiformes) with increasing commercial value, which has prompted active genomic research aimed at more efficient selection. Here we present the sequence and annotation of the turbot genome, which represents a milestone for both boosting breeding programmes and ascertaining the origin and diversification of flatfish. We compare the turbot genome with model fish genomes to investigate teleost chromosome evolution. We observe a conserved macrosyntenic pattern within Percomorpha and identify large syntenic blocks within the turbot genome related to the teleost genome duplication. We identify gene family expansions and positive selection of genes associated with vision and metabolism of membrane lipids, which suggests adaptation to demersal lifestyle and to cold temperatures, respectively. Our data indicate a quick evolution and diversification of flatfish to adapt to benthic life and provide clues for understanding their controversial origin. Moreover, we investigate the genomic architecture of growth, sex determination and disease resistance, key traits for understanding local adaptation and boosting turbot production, by mapping candidate genes and previously reported quantitative trait loci. The genomic architecture of these productive traits has allowed the identification of candidate genes and enriched pathways that may represent useful information for future marker-assisted selection in turbotThis work was funded by the Spanish Government: projects Consolider
Ingenio: Aquagenomics (CSD2007-00002) and Metagenoma
de la Península Ibérica (CSD2007-00005), Ministerio de Economía
y Competitividad and European Regional Development Funds
(AGL2012-35904), and Ministerio de Economía y Competitividad
(AGL2014-51773 and AGL2014-57065-R); and Local Government
Xunta de Galicia (GRC2014/010). P.P. and D.R. gratefully acknowledge
the Spanish Ministerio de Educación for their FPU fellowships
(AP2010-2408, AP2012-0254). Funding to pay the Open Access publication
charges for this article was provided by the Ministerio de
Economía y Competitividad (AGL2014-51773) and Xunta de Galicia
(GRC2014/010)Peer reviewe