Untersuchungen zu phr2AB, einer regulatorischen Untereinheit der Proteinphosphatase PP2A, im Modellorganismus Dictyostelium discoideum

Die regulatorische PP2A Untereinheit phr2AB ist das D. discoideum Ortholog von B55, der regulatorischen Untereinheit in Säugern. Sie wurde als Bindepartner des zentrosomalen Proteins CEP161 identifiziert. Zielsetzung dieser Arbeit ist, die Interaktion mit unabhängigen Methoden zu bestätigen und zu untersuchen, ob diese Interaktion direkt oder indirekt ist. Darüberhinaus soll herausgearbeitet werden, an welchen Bereich von CEP161 phr2AB bindet. In Immunfluorezenzanalysen soll die Lokalisation von phr2AB festgestellt werden. Schließlich sollen die Konsequenzen einer Überexpression von phr2AB als GFP-Fusionsprotein auf Eigenschaften von D. discoideum analysiert werden

Mid-IR spectroscopy of T Tauri stars in Chamealeon I: evidence for processed dust at the earliest stages

We present mid-IR spectroscopy of three T Tauri stars in the young Chamealeon I dark cloud obtained with TIMMI2 on the ESO 3.6m telescope. In these three stars, the silicate emission band at 9.7 micron is prominent. We model it with a mixture of amorphous olivine grains of different size, crystalline silicates and silica. The fractional mass of these various components change widely from star to star. While the spectrum of CR Cha is dominated by small amorphous silicates, in VW Cha (and in a lesser degree in Glass I), there is clear evidence of a large amount of processed dust in the form of crystalline silicates and large amorphous grains. This is the first time that processed dust has been detetected in very young T Tauri stars (~ 1 Myr).Comment: 5 pages, 4 Postscript figures. accepted for A&A Letter

Chemical evolution of a protoplanetary disk

In this paper we review recent progress in our understanding of the chemical evolution of protoplanetary disks. Current observational constraints and theoretical modeling on the chemical composition of gas and dust in these systems are presented. Strong variations of temperature, density, high-energy radiation intensities in these disks, both radially and vertically, result in a peculiar disk chemical structure, where a variety of processes are active. In hot, dilute and heavily irradiated atmosphere only the most photostable simple radicals and atoms and atomic ions exist, formed by gas-phase processes. Beneath the atmosphere a partly UV-shielded, warm molecular layer is located, where high-energy radiation drives rich ion-molecule and radical-radical chemistry, both in the gas phase and on dust surfaces. In a cold, dense, dark disk midplane many molecules are frozen out, forming thick icy mantles where surface chemistry is active and where complex polyatomic (organic) species are synthesized. Dynamical processes affect disk chemical composition by enriching it in abundances of complex species produced via slow surface processes, which will become detectable with ALMA.Comment: 13 pages, 1 figure, 3 tables, IAU 280 "Molecular Universe" invited pape

The T Tauri star RY Tau as a case study of the inner regions of circumstellar dust disks

We study the inner region of the circumstellar disk around the TTauri star RY Tau. Our aim is to find a physical description satisfying the available interferometric data, obtained with the mid-infrared interferometric instrument at the Very Large Telescope Interferometer, as well as the spectral energy distribution. We also compare the findings with the results of similar studies, including those of intermediate-mass stars. Our analysis is done within the framework of a passive circumstellar disk, which is optionally supplemented by the effects of accretion and an added envelope. To achieve a more consistent and realistic model, we used our continuum transfer code MC3D. In addition, we studied the shape of the 10um silicate emission feature in terms of the underlying dust population, both for single-dish and for interferometric measurements. We show that a modestly flaring disk model with accretion can explain both the observed spectral energy distribution and the mid-infrared visibilities obtained with the mid-infrared infrared instrument. We found an interesting ambiguity: a circumstellar active disk model with an added envelope, and a lower accretion rate than in the active disk model without envelope, could represent the observations equally as well. This type of model with the envelope should be considered a viable alternative in future models of other TTauri stars. The approach of a disk with a puffed-up inner rim wall and the influence of a stellar companion is also discussed. From the study of the silicate emission feature we see evidence for dust evolution in a TTauri star, with a decreasing fraction of small amorphous and an increasing fraction of crystalline particles closer to the star.Comment: 19 pages, 23 figures; accepted by Astronomy & Astrophysic

Predictors and outcomes in primary depression care (POKAL) – a research training group develops an innovative approach to collaborative care

BACKGROUND: The interdisciplinary research training group (POKAL) aims to improve care for patients with depression and multimorbidity in primary care. POKAL includes nine projects within the framework of the Chronic Care Model (CCM). In addition, POKAL will train young (mental) health professionals in research competences within primary care settings. POKAL will address specific challenges in diagnosis (reliability of diagnosis, ignoring suicidal risks), in treatment (insufficient patient involvement, highly fragmented care and inappropriate long-time anti-depressive medication) and in implementation of innovations (insufficient guideline adherence, use of irrelevant patient outcomes, ignoring relevant context factors) in primary depression care. METHODS: In 2021 POKAL started with a first group of 16 trainees in general practice (GPs), pharmacy, psychology, public health, informatics, etc. The program is scheduled for at least 6 years, so a second group of trainees starting in 2024 will also have three years of research-time. Experienced principal investigators (PIs) supervise all trainees in their specific projects. All projects refer to the CCM and focus on the diagnostic, therapeutic, and implementation challenges. RESULTS: The first cohort of the POKAL research training group will develop and test new depression-specific diagnostics (hermeneutical strategies, predicting models, screening for suicidal ideation), treatment (primary-care based psycho-education, modulating factors in depression monitoring, strategies of de-prescribing) and implementation in primary care (guideline implementation, use of patient-assessed data, identification of relevant context factors). Based on those results the second cohort of trainees and their PIs will run two major trials to proof innovations in primary care-based a) diagnostics and b) treatment for depression. CONCLUSION: The research and training programme POKAL aims to provide appropriate approaches for depression diagnosis and treatment in primary care

Validation Test Plan PRG

The test plans for two real-time simulations and a field test are documented here. Higher A-SMGCS services were tested in the Prague Airport environment. Aims and objectives, experimental factors and the experiment design are descripted here. Furthermore, the necessary metrics and measurements, the hypotheses that can be accepted or rejected based on the measurements made, and the complete test environment. In addition to that scenario specifications are given and requirements for participants and training of participants are determined. Finally, the conduct of the experiments is described and the envisioned analysis methods is outlined. The document follows the E-OCVM guidelines