191 research outputs found
Rapid selection of specific MAP kinase-binders from designed ankyrin repeat protein libraries
We describe here the rapid selection of specific MAP-kinase binders from a combinatorial library of designed ankyrin repeat proteins (DARPins). A combined in vitro/in vivo selection approach, based on ribosome display and the protein fragment complementation assay (PCA), yielded a large number of different binders that are fully functional in the cellular cytoplasm. Ribosome-display selection pools of four successive selection rounds were examined to monitor the enrichment of JNK2-specific DARPins. Surprisingly, only one round of ribosome display with subsequent PCA selection of this pool was necessary to isolate a first specific binder with micromolar affinity. After only two rounds of ribosome-display selection followed by PCA, virtually all DARPins showed JNK2-specific binding, with affinities in the low nanomolar range. The enrichment factor of ribosome display thus approaches 105 per round. In a second set of experiments, similar results were obtained with the kinases JNK1 and p38 as targets. Again, almost all investigated DARPins obtained after two rounds of ribosome display showed specific binding to the targets used, JNK1 or p38. In all three selection experiments the identified DARPins possess very high specificity for the target kinase. Taken together, the combination of ribosome display and PCA selections allowed the identification of large pools of binders at unparalleled speed. Furthermore, DARPins are applicable in intracellular selections and immunoprecipitations from the extract of eukaryotic cell
Extração de minerais por colmos de cinco variedades de cana-planta em três solos.
Determinação da extração de minerais por tonelada de colmos frescos despalhados de cana-planta de ano e meio em três condições edaficas
Temperatura-base e graus-dia acumulados na emergência de cultivares de girassol, tratados com herbicidas, em Campinas/SP.
Rapid selection of specific MAP kinase-binders from designed ankyrin repeat protein libraries
We describe here the rapid selection of specific MAP-kinase binders from a combinatorial library of designed ankyrin repeat proteins (DARPins). A combined in vitro/in vivo selection approach, based on ribosome display and the protein fragment complementation assay (PCA), yielded a large number of different binders that are fully functional in the cellular cytoplasm. Ribosome-display selection pools of four successive selection rounds were examined to monitor the enrichment of JNK2-specific DARPins. Surprisingly, only one round of ribosome display with subsequent PCA selection of this pool was necessary to isolate a first specific binder with micromolar affinity. After only two rounds of ribosome-display selection followed by PCA, virtually all DARPins showed JNK2-specific binding, with affinities in the low nanomolar range. The enrichment factor of ribosome display thus approaches 105 per round. In a second set of experiments, similar results were obtained with the kinases JNK1 and p38 as targets. Again, almost all investigated DARPins obtained after two rounds of ribosome display showed specific binding to the targets used, JNK1 or p38. In all three selection experiments the identified DARPins possess very high specificity for the target kinase. Taken together, the combination of ribosome display and PCA selections allowed the identification of large pools of binders at unparalleled speed. Furthermore, DARPins are applicable in intracellular selections and immunoprecipitations from the extract of eukaryotic cell
On two problems in graph Ramsey theory
We study two classical problems in graph Ramsey theory, that of determining
the Ramsey number of bounded-degree graphs and that of estimating the induced
Ramsey number for a graph with a given number of vertices.
The Ramsey number r(H) of a graph H is the least positive integer N such that
every two-coloring of the edges of the complete graph contains a
monochromatic copy of H. A famous result of Chv\'atal, R\"{o}dl, Szemer\'edi
and Trotter states that there exists a constant c(\Delta) such that r(H) \leq
c(\Delta) n for every graph H with n vertices and maximum degree \Delta. The
important open question is to determine the constant c(\Delta). The best
results, both due to Graham, R\"{o}dl and Ruci\'nski, state that there are
constants c and c' such that 2^{c' \Delta} \leq c(\Delta) \leq 2^{c \Delta
\log^2 \Delta}. We improve this upper bound, showing that there is a constant c
for which c(\Delta) \leq 2^{c \Delta \log \Delta}.
The induced Ramsey number r_{ind}(H) of a graph H is the least positive
integer N for which there exists a graph G on N vertices such that every
two-coloring of the edges of G contains an induced monochromatic copy of H.
Erd\H{o}s conjectured the existence of a constant c such that, for any graph H
on n vertices, r_{ind}(H) \leq 2^{c n}. We move a step closer to proving this
conjecture, showing that r_{ind} (H) \leq 2^{c n \log n}. This improves upon an
earlier result of Kohayakawa, Pr\"{o}mel and R\"{o}dl by a factor of \log n in
the exponent.Comment: 18 page
Influência do EPTC no desenvolvimento e absorção de macronutrientes pela cultura de feijão (Phaseolus vulgaris L. var. Carioca)
Improving manual oxygen titration in preterm infants by training and guideline implementation
To study oxygen saturation (SpO2) targeting before and after training and guideline implementation of manual oxygen titration, two cohorts of preterm infants 21%. ABCs where oxygen therapy was given were identified and analyzed. After training and guideline implementation the %SpO2-wtr increased (median interquartile range (IQR)) 48.0 (19.6-63.9) % vs 61.9 (48.5-72.3) %; p 95% (44.0 (27.8-66.2) % vs 30.8 (22.6-44.5) %; p 95% did not decrease (73% vs 64%; ns) but lasted shorter (2 (0-7) vs 1 (1-3) minute; p < 0.004). CONCLUSION: Training and guideline implementation in manual oxygen titration improved SpO2 targeting in preterm infants with more time spent within the target range and less frequent hyperoxaemia. The durations of hypoxaemia and hyperoxaemia during ABCs were shorter. What is Known: • Oxygen saturation targeting in preterm infants can be challenging and the compliance is low when oxygen is titrated manually. • Hyperoxaemia often occurs after oxygen therapy for oxygen desaturation during apnoeas. What is New: • Training and implementing guidelines improved oxygen saturation targeting and reduced hyperoxaemia. • Training and implementing guidelines improved manual oxygen titration during ABC
-WAVVAP) campaign
[1] We present a validation study for the ground-based Middle Atmospheric Water Vapour Radiometer (MIAWARA) operating at 22 GHz. MIAWARA measures the water vapor profile in the range of 20-80 km. The validation was conducted in two phases at different geographical locations. During the first operational period the radiometer was operated at middle latitudes in Bern, Switzerland, and the measured water vapor profiles were compared with the HALOE satellite instrument. The agreement between HALOE and MIAWARA was for most altitudes better than 10%. The agreement between the balloon instruments and MIAWARA was better than 2% for a total number of 10 comparable flights. This showed the potential of MIAWARA in water vapor retrieval down to 20 km. In addition, the northern Finland MIAWARA profiles were compared with POAM III water vapor profiles. This comparison confirmed the good agreement with the other instruments, and the difference between MIAWARA and POAM was generally less than 8%. Finally, the tipping curve calibration was validated with tipping curve measurements of the All-Sky Multi Wavelength Radiometer (ASMUWARA) which was operated 10 months side by side with MIAWARA. The agreement of the tropospheric opacity derived from these tipping curves agree within 1%
Validation of water vapour profiles (version 13) retrieved by the IMK/IAA scientific retrieval processor based on full resolution spectra measured by MIPAS on board Envisat
Vertical profiles of stratospheric water vapour measured by the Michelson Interferometer for Passive Atmospheric Sounding (MIPAS) with the full resolution mode between September 2002 and March 2004 and retrieved with the IMK/IAA scientific retrieval processor were compared to a number of independent measurements in order to estimate the bias and to validate the existing precision estimates of the MIPAS data. The estimated precision for MIPAS is 5 to 10% in the stratosphere, depending on altitude, latitude, and season. The independent instruments were: the Halogen Occultation Experiment (HALOE), the Atmospheric Chemistry Experiment Fourier Transform Spectrometer (ACE-FTS), the Improved Limb Atmospheric Spectrometer-II (ILAS-II), the Polar Ozone and Aerosol Measurement (POAM III) instrument, the Middle Atmospheric Water Vapour Radiometer (MIAWARA), the Michelson Interferometer for Passive Atmospheric Sounding, balloon-borne version (MIPAS-B), the Airborne Microwave Stratospheric Observing System (AMSOS), the Fluorescent Stratospheric Hygrometer for Balloon (FLASH-B), the NOAA frostpoint hygrometer, and the Fast In Situ Hygrometer (FISH). For the in-situ measurements and the ground based, air- and balloon borne remote sensing instruments, the measurements are restricted to central and northern Europe. The comparisons to satellite-borne instruments are predominantly at mid- to high latitudes on both hemispheres. In the stratosphere there is no clear indication of a bias in MIPAS data, because the independent measurements in some cases are drier and in some cases are moister than the MIPAS measurements. Compared to the infrared measurements of MIPAS, measurements in the ultraviolet and visible have a tendency to be high, whereas microwave measurements have a tendency to be low. The results of &chi;<sup>2</sup>-based precision validation are somewhat controversial among the comparison estimates. However, for comparison instruments whose error budget also includes errors due to uncertainties in spectrally interfering species and where good coincidences were found, the &chi;<sup>2</sup> values found are in the expected range or even below. This suggests that there is no evidence of systematically underestimated MIPAS random errors
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