Studies on Extractive Fermentation for L-Phenylalanine Production by Recombinant Escherichia coli Immobilized with Microcapsules

作为人体八种必需氨基酸之一的L-苯丙氨酸(L-Phenylalanine,L-Phe)，是一种重要的合成中间体，在食品和医药领域有广泛的应用，可作为食品添加剂、抗癌药物中间体和合成阿斯巴甜的原料，具有非常广阔的市场前景。利用固定化重组大肠杆菌细胞发酵生产L-Phe，是当前制备L-Phe应用最广泛的一种方法。本文利用两种新型微胶囊——SA-CMC/CaCl2微胶囊体系与NaCS-PDMDAAC微胶囊体系对重组大肠杆菌进行固定化，探索了它们在萃取发酵生产L-Phe过程中的特性，并与游离细胞培养作了比较，试图对实验室探索L-Phe的工艺以及大规模生产L-Phe提供理论依据。论文先对有机溶液萃取分离L...As one of the eight amino acids essential to human being, L-Phenylalanine (L-Phe) is an important organic compound intermediate, which can be widely used in food industry, medicine, and chemical engineering. It is a major food additive and anticancer medicament intermediate. What is more, it is the raw material of aspartame synthesis, which made it widely demanded in the market. Fermentation by...学位：工程硕士院系专业：化学化工学院化学工程与生物工程系_生物化工学号：20043300

人民陪审员制度中法律审与事实审分离机制研究

目前人民陪审员参审案件的职权包括了法律审和事实审,并不能充分发挥人民陪审员的优势,也不符合司法实践的需求,甚至是造成人民陪审员"陪而不审,审而不议"的主要原因。司法改革稳步推进,对人民陪审员制度的改革也拉开帷幕。结合改革实践和域外法比较,并对人民陪审员制度中法律审和事实审的分离机制进行分析可知,探索重大案件由3名以上人以陪审员参加会议庭机制、切实推进事实认定问题清单列表制度以及强化人民陪审员对事实认定的责任制度是切实可行的

Test and Significance of CEA, NSE, CA125, CYFRA21-1 in Lung Cancer

目的 探讨CEA、NSE、CA125、CYFRA21-1在肺癌中的检测及意义.方法 回顾性分析2015年10月—2016年12月医院收治的肺部疾病患者166例,其中89例肺癌患者设为肺癌组,其余77例肺部良性病变患者设为肺部良性病变组.另选择35名健康志愿者作为健康对照组,电化学发光法检测3组血清中CEA、NSE、CA125、CYFRA21-1含量,分析单项及联合检测对肺癌的诊断价值.结果 肺癌组患者血清CEA、NSE、CA125、CYFRA21-1分别为（4.32±0.83）ng/mL、（11.54±4.17）ng/mL、（37.89±6.69）U/mL、（4.02±0.56）ng/mL,均高于肺部良性病变组和健康对照组,差异有统计学意义（P〈0.05）.肺部良性病变组和健康对照组血清CEA、NSE、CA125、CYFRA21-1比较差异无统计学意义（P〉0.05）.CEA、NSE、CA125、CYFRA21-1单项诊断肺癌的敏感性偏低,特异度较高;而四项联合检测诊断肺癌的敏感性显著升高,为87.64%,差异有统计学意义（P〈0.05）;特异度略有降低,差异无统计学意义（P〉0.05）.结论 肺癌患者血清中CEA、NSE、CA125、CYFRA21-1升高,四项联合检测可以提高对肺癌的诊断准确性.Objective To study the test and significance of CEA, NSE, CA125, CYFRA21-1 in lung cancer. Methods 166 cases of patients with lung diseases admitted and treated in our hospital from October 2015 to December 2016 were selected and divided into the lung cancer group with 89 cases and lung benign lesion group with 77 cases in each, and 35 cases of healthy volunteers were selected as the control group, and the contents of CEA,NSE,CA125,CYFRA21- 1 in serum of the three groups were tested by the electrochemiluminescence, and the diagnosis value of single and com- bined test for lung cancer was analyzed. Results The serum CEA,NSE,CA125,CYFRA21-1 in the lung cancer group were respectively （4.32±0.83）ng/mL, （ll.54±4.17）ng/mL, （37.89±6.69）U/mL, （4.02±0.56）ng/mL; which were higher than those in the lung benign lesion group and healthy control group, and the differences were statistically significant （P〈0.05）, and the differences in the CEA,NSE,CA125,CYFRA21-1 in serum between the lung benign lesion group and healthy control group were not statistically significant （P〉0.05）, and the sensitivity of single diagnosis of CEA,NSE, CA125,CYFRA21-1 for lung cancer was lower and the specificity was higher, but the sensitivity of four combined test in diagnosis of lung cancer .obviously increased, （87.64%）, and the difference was statistically significant（P〈0.05）, the specificity slightly decreased, and the difference was not statistically significant （/9〉0.05）. Conclusion The CEA, NSE, CA125, CYFRA21-1 in serum of patients with lung cancer increase, and the four combined test can improve the diag-nostic accuracy of lung cancer

糖化血红蛋白的应用价值研究进展

糖化血红蛋白(HbA_(1C))是人体血液中红细胞内的血红蛋白与血糖结合的产物。属于不可逆反应过程,并与血糖浓度成正比,且保持3~4个月左右,所以可以检测到120天之前的血糖浓度~([1-2])。因此HbA_(1C)水平在一定程度上反映患者近8~12周的血糖水平。近年来,HbA_(1C)越来越受到国内外相关人士的重视,美国糖尿病协会(ADA)于2011

表面增强拉曼光谱法在食品中亚硝酸盐的快速筛查研究

建立了采用表面增强拉曼光谱对香肠等食品中亚硝酸盐的快速筛查方法。检测样品经简单提取、衍生后直接进行拉曼光谱检测,整个前处理时间不超过15分钟。通过亚硝酸盐衍生物的指纹图谱定性,本方法能够对各种食品中的亚硝酸盐含量准确筛查,检出限为30 mg/kg,能够满足国家卫生标准的限量要求。该方法前处理简便快速,结果准确,可以作为一种高灵敏、无假阳性的非定向快速筛查手段。该方法可用于检测食品中亚硝酸盐的滥用添加,满足职能部门的现场执法和实验室预检需求。国家自然科学基金（21703187）厦门大学校长基金（20720170102）江苏省产学研前瞻项目（BY2018200

Optimization of Conditions for Producing L-Phenylalanine by Rhodotorula Glutinis Immobilized in Microcapsule Using Response Surface Methodology

以微胶囊固定化粘红酵母为研究对象,考察了粘红酵母转化反式肉桂酸(T-CA)生成l-苯丙氨酸过程中转化体系温度、PH值、CnH+4/CT-CA对l-苯丙氨酸积累量的影响,并通过响应面法对转化条件进行了优化,得到最佳转化条件为:温度30.75℃、PH值11.58、CnH+4=4.77MOl.l-1、CT-CA=10g·l-1,在该条件下,l-苯丙氨酸积累量为27.5g·l-1。Taking Rhodotorula glutinis immobilized in microcapsule as the research object,the effects of temperature,pH value,NH+4 concentration and t-CA concentration on L-Phe yield using t-CA as substrate by Rhodotorula glutinis were investigated.The parameters of reaction were optimized by response surface methodology.The optimum conditions were obtained as follows:temperature was 30.75 ℃,pH value was 11.58,NH+4 concentration was 4.77 mol·L-1,t-CA concentration was 10 g·L-1.The maximal L-Phe yield was 27.5 g·L-1 under the optimum conditions

Separation of L-phenylalanine by Organic Solvent Extraction

以磷酸二(2-乙基己基)脂(D2EHPA)为萃取剂,环己烷为稀释剂萃取L-苯丙氨酸(L-Phe),研究了L-Phe浓度、D2EHPA浓度、温度对萃取分配系数D的影响,以及负载L-Phe有机相的反萃取特性.结果表明:萃取分配系数D随温度的升高而降低,而L-Phe浓度对分配系数的影响还与水相初始pH值有关;萃合物是以一个L-Phe分子和二个D2EHPA分子结合而成.在利用酸溶液对负载L-Phe有机相的反萃取过程中,反萃取的效果符合HCl>HNO3≥H2SO4>CH3COOH,且随HCl浓度和温度的升高而升高.The extraction of L-Phenylalanine(L-Phe) with di-(2-ethylhexyl)phosphoric acid(D2EHPA) as extractant and cyclohexane as diluent was studied.The factors that influence the L-Phe extraction process such as the concentration of L-Phe and D2EHPA,and the temperature,as well as back-extraction,were investigated.The results showed that the distribution coefficient decreased with increasing temperature and depended on the concentration of L-Phe and the initial pH value of aqueous phase.The study of the mechanism of the extraction process indicated that the extraction complex might be composed of one L-Phe molecule and two D2EHPA molecules.The effect of acids on the back-extraction process of L-Phe was proved to be HCl>HNO3≥H2SO4>CH3COOH,and the back-extraction ratio increased with increasing HCl concentration and temperature.厦门大学新世纪优秀人才支持计划(X07161)资

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies