Studies on Extractive Fermentation for L-Phenylalanine Production by Recombinant Escherichia coli Immobilized with Microcapsules

Abstract

作为人体八种必需氨基酸之一的L-苯丙氨酸(L-Phenylalanine,L-Phe),是一种重要的合成中间体,在食品和医药领域有广泛的应用,可作为食品添加剂、抗癌药物中间体和合成阿斯巴甜的原料,具有非常广阔的市场前景。利用固定化重组大肠杆菌细胞发酵生产L-Phe,是当前制备L-Phe应用最广泛的一种方法。本文利用两种新型微胶囊——SA-CMC/CaCl2微胶囊体系与NaCS-PDMDAAC微胶囊体系对重组大肠杆菌进行固定化,探索了它们在萃取发酵生产L-Phe过程中的特性,并与游离细胞培养作了比较,试图对实验室探索L-Phe的工艺以及大规模生产L-Phe提供理论依据。论文先对有机溶液萃取分离L...As one of the eight amino acids essential to human being, L-Phenylalanine (L-Phe) is an important organic compound intermediate, which can be widely used in food industry, medicine, and chemical engineering. It is a major food additive and anticancer medicament intermediate. What is more, it is the raw material of aspartame synthesis, which made it widely demanded in the market. Fermentation by...学位:工程硕士院系专业:化学化工学院化学工程与生物工程系_生物化工学号:20043300

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