"We can remember it for you": location, memory, and commodification in social networking sites

This article explores the spatial self through the performative aspects of location sharing and geotagging in the process of self-representation on social networking sites (SNSs). Based on the legacy of early experimentations with location-based technologies for social interaction, the article asserts that the representation of location in SNSs has more temporal than spatial attributes. The article explores the immediacy of networks and the different kinds of temporality encountered in SNSs to address the commodification of geotagged content uploaded on SNSs. Location-based data are valuable commodities bought and sold in the market. Therefore, the act of archiving memories on SNSs is commodified and performed within the predetermined functions and actions set within the SNSs’ interfaces. SNSs devise ways to keep users constantly interacting with the present moment in time and simultaneously create memories of the recent past while disclosing personal data that companies use for profit

Nuclear anomalies in the buccal cells of calcite factory workers

The micronucleus (MN) assay on exfoliated buccal cells is a useful and minimally invasive method for monitoring genetic damage in humans. To determine the genotoxic effects of calcite dust that forms during processing, MN assay was carried out in exfoliated buccal cells of 50 (25 smokers and 25 non-smokers) calcite factory workers and 50 (25 smokers and 25 non-smokers) age- and sex-matched control subjects. Frequencies of nuclear abnormalities (NA) other than micronuclei, such as binucleates, karyorrhexis, karyolysis and ‘broken eggs', were also evaluated. Micronuclei and the other aforementioned anomalies were analysed by two way analysis of covariance. The linear correlations between the types of micronucleus and nuclear abnormalities were determined by Spearman's Rho. There was a positive correlation between micronuclei and other types of nuclear abnormalities in accordance with the Spearman's Rho test. Results showed statistically significant difference between calcite fabric workers and control groups. MN and NA frequencies in calcite fabric workers were significantly higher than those in control groups (p < 0.05). The results of this study indicate that calcite fabric workers are under risk of significant cytogenetic damage

Co-circulation of West Nile virus and distinct insect-specific flaviviruses in Turkey

Background: Active vector surveillance provides an efficient tool for monitoring the presence or spread of emerging or re-emerging vector-borne viruses. This study was undertaken to investigate the circulation of flaviviruses. Mosquitoes were collected from 58 locations in 10 provinces across the Aegean, Thrace and Mediterranean Anatolian regions of Turkey in 2014 and 2015. Following morphological identification, mosquitoes were pooled and screened by nested and real-time PCR assays. Detected viruses were further characterised by sequencing. Positive pools were inoculated onto cell lines for virus isolation. Next generation sequencing was employed for genomic characterisation of the isolates. Results: A total of 12,711 mosquito specimens representing 15 species were screened in 594 pools. Eleven pools (2%) were reactive in the virus screening assays. Sequencing revealed West Nile virus (WNV) in one Culex pipiens (s.l.) pool from Thrace. WNV sequence corresponded to lineage one clade 1a but clustered distinctly from the Turkish prototype isolate. In 10 pools, insect-specific flaviviruses were characterised as Culex theileri flavivirus in 5 pools of Culex theileri and one pool of Cx. pipiens (s.l.), Ochlerotatus caspius flavivirus in two pools of Aedes (Ochlerotatus) caspius, Flavivirus AV-2011 in one pool of Culiseta annulata, and an undetermined flavivirus in one pool of Uranotaenia unguiculata from the Aegean and Thrace regions. DNA forms or integration of the detected insect-specific flaviviruses were not observed. A virus strain, tentatively named as “Ochlerotatus caspius flavivirus Turkey”, was isolated from an Ae. caspius pool in C6/36 cells. The viral genome comprised 10,370 nucleotides with a putative polyprotein of 3,385 amino acids that follows the canonical flavivirus polyprotein organisation. Sequence comparisons and phylogenetic analyses revealed the close relationship of this strain with Ochlerotatus caspius flavivirus from Portugal and Hanko virus from Finland. Several conserved structural and amino acid motifs were identified. Conclusions: We identified WNV and several distinct insect-specific flaviviruses during an extensive biosurveillance study of mosquitoes in various regions of Turkey in 2014 and 2015. Ongoing circulation of WNV is revealed, with an unprecedented genetic diversity. A probable replicating form of an insect flavivirus identified only in DNA form was detected

[A. Rıza Özkul tarafından Fethi İsfendiyaroğlu'na gönderilen mektup]

Taha Toros Arşivi, Dosya No: Yabancı Gözüyle İstanbul. Not: Ekte A. Rıza Özkul'un Fethi İsfendiyaroğlu'na yaptığı tercüme metni de bulunmaktadır.Unutma İstanbul projesi İstanbul Kalkınma Ajansı'nın 2016 yılı "Yenilikçi ve Yaratıcı İstanbul Mali Destek Programı" kapsamında desteklenmiştir. Proje No: TR10/16/YNY/010

Biotypic characterisation of bovine viral diarrhoea virus (BVDV) using reverse transcription-polymerase chain reaction (RT-PCR) in clinical samples

In this study, bovine viral diarrhoea virus (BVDV) was detected and biotypically characterised in clinical samples using reverse transcriptase-polymerase chain reaction (RT-PCR). The RT-PCR technique produced two different amplicons (402 and approx. 680 bp in size) in case of the presence of both biotypes (cp and ncp) in the sample. The mixture of the biotypes as detected by RT-PCR was verified by the immunoplaque assay (IPA). Purification of biotypes was carried out by native plaque isolation and subsequent RT-PCR revealed single products (402 or approx. 680 bp in size) in each clone. The results showed that RT-PCR can be used for accurate molecular differentiation between the BVDV biotypes

Investigations on viral aetiology of diarrhea in puppies.

In this research, the role of Canine Parvovirus-2 (CPV-2), Canine Corona Virus (CCoV), Canine Distemper Virus (CDV) and Canine Rotavirus (CRV) were investigated as an aetiological agent in diarrheic puppies. For that purpose, faeces sampled from 90 diarrheic puppies were evaluated for CPV-2 by hemagglütination reaction and for CCoV genom by reverse transcription-polymerase chain reaction (PCR). Twenty five of these samples were tested for the presence of CDV genome by RT- PCR and for the presence of CRV genomic segments by polyacrilamide gel electrophoresis (PAGE). On the other hand faeces from 14 puppies with respiratory and enteric symptoms were also tested for CDV. All of animals sampled were aged between 0-4 months old. Thirty two (35.5%) among 90 samples were positive for CPV-2, and 14 (15.5) samples were positive for CCoV genome. None of these samples had cross positivity for both viruses. Out of 39 samples tested for CDV, 15 (38.4%) were positive for CDV genom. There was no faecal sample containing CRV genomic segments. Results of this study demonstrate that CPV-2 is the most prevalent viral agent in clinical diarrhea cases of puppies, and is followed by CCoV and CDV virus. It can be clearly said that, these three viruses were at least related to 58.6% of diarrhea cases in the age group investigated