Favourable areas for co-occurrence of parapatric species: niche conservatism and niche divergence in Iberian tree frogs and midwife toads

Aim Predicting species responses to global change is one of the most pressing issues in conservation biogeography. A key part of the problem is understanding how organisms have reacted to climatic changes in the past. Here we use species distribution modelling to infer the effects of climate changes since the Last Interglacial (LIG, about 130,000 ybp) on patterns of genetic structure and diversity in the Western Spadefoot toad (Pelobates cultripes) in combination with spatially-explicit phylogeographic analyses. Location Iberian Peninsula and mainland France. Methods 524 individuals from 54 populations across the species range were sampled to document patterns of genetic diversity and infer their evolutionary history based on data from mtDNA and fourteen polymorphic microsatellites. Generalized linear models based on distribution data were used to infer climatic favourability for the species in the present and in paleoclimatic simulations for the LIG, the Mid Holocene and the last glacial maximum (LGM). Results Estimates of genetic diversity show a decreasing trend from south to north, suggesting persistence of high historical population sizes in the southern Iberian Peninsula. Species distribution models show differences in climatic favourability through time, with significant correlations between historically stable favourable areas and current patterns of genetic diversity. These results are corroborated by Bayesian Skyline Plots and continuous diffusion phylogeographic analyses. Main conclusions The results indicate the presence of southern refugia, with moderate recent expansions at the northern end of the species’ range. Toads at the northern range margin exhibit the lowest genetic diversity and occupy areas of high past climate variability, classified as marginal in terms of favourability, rendering these populations most vulnerable to climate-mediated changes in the long term

Amphibian Metacommunity Responses to Agricultural Intensification in a Mediterranean Landscape

Agricultural intensification has been associated with biodiversity declines, habitat fragmentation and loss in a number of organisms. Given the prevalence of this process, there is a need for studies clarifying the effects of changes in agricultural practices on local biological communities; for instance, the transformation of traditional rainfed agriculture into intensively irrigated agriculture. We focused on pond-breeding amphibians as model organisms to assess the ecological effects of agricultural intensification because they are sensitive to changes in habitat quality at both local and landscape scales. We applied a metacommunity approach to characterize amphibian communities breeding in a network of ponds embedded in a terrestrial habitat matrix that was partly converted from rainfed crops to intensive irrigated agriculture in the 1990s. Specifically, we compared alpha and beta diversity, species occupancy and abundance, and metacommunity structure between irrigated and rainfed areas. We found strong differences in patterns of species occurrence, community structure and pairwise beta diversity between agricultural management groups, with a marked community structure in rainfed ponds associated with local features and the presence of some rare species that were nearly absent in the irrigated area, which was characterized by a random community structure. Natural vegetation cover at the landscape scale, significantly lower on the irrigated area, was an important predictor of species occurrences. Our results suggest that maintaining both local and landscape heterogeneity is key to preserving diverse amphibian communities in Mediterranean agricultural landscapes.This research was funded by the Spanish Ministerio de Economía, Industria y Competitividad, project Metaponds, (ref: CGL2017-84176-R); and by Fundación Biodiversidad, Ministerio para la Transición Ecológica y el Reto Demográfico (project BT-2019). L. Albero is funded by a Phd grant from Universidad de León (ULE).Peer reviewe

Ammonoids of the Oxynotum Zone and Raricostatum Zone (Densinodulum Subzone) of Sinemurian, Lower Jurassic, in the Asturian and Lusitanian basins

This research is focused on the ammonoids of Oxynotum Zone and Raricostatum Zone (Densinodulum Subzone) from Asturian (Spain) and Lusitanian (Portugal) basins. They come from expanded sections from where 216 specimens have been collected. These have been classified in eight genera and 15 species that belong to families Echioceratidae, Oxynoticeratidae and Eoderoceratidae. Most of the ammonoids are typical of the Northwest European Province. Echioceratidae is the most frequent in the Asturian Basin, whereas Oxynoticeratidae is the predominant in the Lusitanian Basin. Eoderoceratidae specimens are scarcer, and they are registered only between the middle part of Oxynotum Subzone and the lower part of Densinodulum Subzone. A new species is proposed for the upper part of Oxynotum Subzone of the Lusitanian Basin, Plesechioceras rochai sp. nov., which is relatively frequent. As well, also in Portugal, Cheltonia sp. is registered in Densinodulum Subzone, which is a form different from Cheltonia accipitris. This provides new evidence to support the hypothesis of the relation micro-macroconch between the genera Oxynoticeras (M) and Cheltonia (m). Remarkable differences in the studied area are the presence of Paroxynoticeras salisburgense and Plesechioceras cf. delicatum in the Asturias Basin, and Crucilobiceras densinodulum, associated to Oxynoticeras lymense, in the Lusitanian Basin

Malope trifida Cav. (Malvaceae) dans la province de Séville, près de trente ans après son dernier signalement.

New records of Malope trifida are reported in the province of Seville (southern Spain). His current presence is verified since his last testimony was recorded in 1990, data on its ecology are provided and its regional distribution is expanded.Se comunican nuevas citas de Malope trifida en la provincia de Sevilla (sur de España). Se verifica su presencia actual desde que se tuviera constancia de su último testimonio en 1990, se aportan datos sobre su ecología y se amplía su distribución regional.De nouveaux signalements de Malope trifida sont signalés dans la province de Séville (sud de l'Espagne). Sa présence actuelle est vérifiée puisque son dernier témoignage a été enregistré en 1990, des données sur son écologie sont fournies et sa répartition régionale est élargie

What are the topics you care about making trials in lupus more effective? Results of an Open Space meeting of international lupus experts

Despite promising candidates for new therapeutic options in the treatment of systemic lupus erythematosus (SLE), many clinical trials have failed in the past few years. The disappointing results have been at least partly be attributed to trial designs. With the aim of stimulating new developments in SLE trial design, an international open space meeting was held on occasion of the European Lupus Meeting 2018 in Duesseldorf, Germany about ‘What are the topics you care about for making trials in lupus more effective?’. The Open Space is a participant-driven technology, where the discussion topics and schedule are selected during the meeting by all participants and discussion rounds are led by the people attending encouraging active contributions. Eleven topics were selected for further discussion, of which 6 were voted to be more intensively discussed in two consecutive rounds. Major topics were the optimal handling of glucocorticoids in clinical trials, the improvement of outcome measures, reducing or controlling the placebo response and the identification of biomarkers and stratification parameters. Further, the importance of local and international networks was emphasised. By networking, collaborations are facilitated, patient recruitment is more efficient and treatment can be harmonised thus lead to more successful SLE trials. Further discussions are needed to substantiate the results and develop new trial designs

Impacto de la COVID-19 en el tratamiento del infarto agudo de miocardio con elevación del segmento ST. La experiencia Española

The COVID-19 outbreak has had an unclear impact on the treatment and outcomes of patients with ST-segment elevation myocardial infarction (STEMI). The aim of this study was to assess changes in STEMI management during the COVID-19 outbreak. Using a multicenter, nationwide, retrospective, observational registry of consecutive patients who were managed in 75 specific STEMI care centers in Spain, we compared patient and procedural characteristics and in-hospital outcomes in 2 different cohorts with 30-day follow-up according to whether the patients had been treated before or after COVID-19. Suspected STEMI patients treated in STEMI networks decreased by 27.6% and patients with confirmed STEMI fell from 1305 to 1009 (22.7%). There were no differences in reperfusion strategy (> 94% treated with primary percutaneous coronary intervention in both cohorts). Patients treated with primary percutaneous coronary intervention during the COVID-19 outbreak had a longer ischemic time (233 [150-375] vs 200 [140-332] minutes, P < .001) but showed no differences in the time from first medical contact to reperfusion. In-hospital mortality was higher during COVID-19 (7.5% vs 5.1%; unadjusted OR, 1.50; 95%CI, 1.07-2.11; P < .001); this association remained after adjustment for confounders (risk-adjusted OR, 1.88; 95%CI, 1.12-3.14; P = .017). In the 2020 cohort, there was a 6.3% incidence of confirmed SARS-CoV-2 infection during hospitalization. The number of STEMI patients treated during the current COVID-19 outbreak fell vs the previous year and there was an increase in the median time from symptom onset to reperfusion and a significant 2-fold increase in the rate of in-hospital mortality. No changes in reperfusion strategy were detected, with primary percutaneous coronary intervention performed for the vast majority of patients. The co-existence of STEMI and SARS-CoV-2 infection was relatively infrequent.S

The maternal genetic make-up of the Iberian Peninsula between the Neolithic and the Early Bronze Age

Abstract Agriculture first reached the Iberian Peninsula around 5700 BCE. However, little is known about the genetic structure and changes of prehistoric populations in different geographic areas of Iberia. In our study, we focus on the maternal genetic makeup of the Neolithic (~ 5500–3000 BCE), Chalcolithic (~ 3000–2200 BCE) and Early Bronze Age (~ 2200–1500 BCE). We report ancient mitochondrial DNA results of 213 individuals (151 HVS-I sequences) from the northeast, central, southeast and southwest regions and thus on the largest archaeogenetic dataset from the Peninsula to date. Similar to other parts of Europe, we observe a discontinuity between hunter-gatherers and the first farmers of the Neolithic. During the subsequent periods, we detect regional continuity of Early Neolithic lineages across Iberia, however the genetic contribution of hunter-gatherers is generally higher than in other parts of Europe and varies regionally. In contrast to ancient DNA findings from Central Europe, we do not observe a major turnover in the mtDNA record of the Iberian Late Chalcolithic and Early Bronze Age, suggesting that the population history of the Iberian Peninsula is distinct in character

Taking the pulse of Earth\u27s tropical forests using networks of highly distributed plots

Tropical forests are the most diverse and productive ecosystems on Earth. While better understanding of these forests is critical for our collective future, until quite recently efforts to measure and monitor them have been largely disconnected. Networking is essential to discover the answers to questions that transcend borders and the horizons of funding agencies. Here we show how a global community is responding to the challenges of tropical ecosystem research with diverse teams measuring forests tree-by-tree in thousands of long-term plots. We review the major scientific discoveries of this work and show how this process is changing tropical forest science. Our core approach involves linking long-term grassroots initiatives with standardized protocols and data management to generate robust scaled-up results. By connecting tropical researchers and elevating their status, our Social Research Network model recognises the key role of the data originator in scientific discovery. Conceived in 1999 with RAINFOR (South America), our permanent plot networks have been adapted to Africa (AfriTRON) and Southeast Asia (T-FORCES) and widely emulated worldwide. Now these multiple initiatives are integrated via ForestPlots.net cyber-infrastructure, linking colleagues from 54 countries across 24 plot networks. Collectively these are transforming understanding of tropical forests and their biospheric role. Together we have discovered how, where and why forest carbon and biodiversity are responding to climate change, and how they feedback on it. This long-term pan-tropical collaboration has revealed a large long-term carbon sink and its trends, as well as making clear which drivers are most important, which forest processes are affected, where they are changing, what the lags are, and the likely future responses of tropical forests as the climate continues to change. By leveraging a remarkably old technology, plot networks are sparking a very modern revolution in tropical forest science. In the future, humanity can benefit greatly by nurturing the grassroots communities now collectively capable of generating unique, long-term understanding of Earth\u27s most precious forests