178 research outputs found

    Remote Sensing for Precision Nitrogen Management

    Get PDF
    This book focuses on the fundamental and applied research of the non-destructive estimation and diagnosis of crop leaf and plant nitrogen status and in-season nitrogen management strategies based on leaf sensors, proximal canopy sensors, unmanned aerial vehicle remote sensing, manned aerial remote sensing and satellite remote sensing technologies. Statistical and machine learning methods are used to predict plant-nitrogen-related parameters with sensor data or sensor data together with soil, landscape, weather and/or management information. Different sensing technologies or different modelling approaches are compared and evaluated. Strategies are developed to use crop sensing data for in-season nitrogen recommendations to improve nitrogen use efficiency and protect the environment

    Detection of Amazon Forest Degradation Caused by Land Use Changes

    Get PDF
    Field and satellite optical methods for estimation of chlorophyll content were applied in three study sites of the Ecuadorian Amazon rainforest. Those sites represent a wide range of land use disturbance in secondary and pristine lowland rainforest. The first field method is based on transmittance from the SPAD-502 chlorophyll meter index, the second field method is based on reflectance measurements collected by a spectroradiometer, and the third method estimates chlorophyll content from the PROSPECT radiative transfer model. For the first method, seven models that account for a wide range of vegetation species showed similar average leaf chlorophyll contents until 80 units of SPAD-502. An average of the results of these models was computed and used as ground truth from where a generalized second-order polynomial model was created. For the second method, five chlorophyll indices based on reflectance measurements provided similar chlorophyll content estimations for all SPAD range (15–95 units). The third method estimates chlorophyll content based on the inversion process of the PROSPECT model. The satellite methods estimate vegetation indices sensitive to chlorophyll content from space. All methods have shown to be an alternative approach to detect forest degradation at local and regional levels caused by forest disturbances and land use changes

    High throughput analysis of leaf chlorophyll content in sorghum using RGB, hyperspectral, and fluorescence imaging and sensor fusion

    Get PDF
    Background: Leaf chlorophyll content plays an important role in indicating plant stresses and nutrient status. Traditional approaches for the quantification of chlorophyll content mainly include acetone ethanol extraction, spectrophotometry and high-performance liquid chromatography. Such destructive methods based on laboratory procedures are time consuming, expensive, and not suitable for high-throughput analysis. High throughput imaging techniques are now widely used for non-destructive analysis of plant phenotypic traits. In this study three imaging modules (RGB, hyperspectral, and fluorescence imaging) were, separately and in combination, used to estimate chlorophyll content of sorghum plants in a greenhouse environment. Color features, spectral indices, and chlorophyll fluorescence intensity were extracted from these three types of images, and multiple linear regression models and PLSR (partial least squares regression) models were built to predict leaf chlorophyll content (measured by a handheld leaf chlorophyll meter) from the image features. Results: The models with a single color feature from RGB images predicted chlorophyll content with R2 ranging from 0.67 to 0.88. The models using the three spectral indices extracted from hyperspectral images (Ration Vegetation Index, Normalized Difference Vegetation Index, and Modified Chlorophyll Absorption Ratio Index) predicted chlorophyll content with R2 ranging from 0.77 to 0.78. The model using the fluorescence intensity extracted from fluorescence images predicted chlorophyll content with R2 of 0.79. The PLSR model that involved all the image features extracted from the three different imaging modules exhibited the best performance for predicting chlorophyll content, with R2 of 0.90. It was also found that inclusion of SLW (Specific Leaf Weight) into the image-based models further improved the chlorophyll prediction accuracy. Conclusion: All three imaging modules (RGB, hyperspectral, and fluorescence) tested in our study alone could estimate chlorophyll content of sorghum plants reasonably well. Fusing image features from different imaging modules with PLSR modeling significantly improved the predictive performance. Image-based phenotyping could provide a rapid and non-destructive approach for estimating chlorophyll content in sorghum

    A Cost-effective Multispectral Sensor System for Leaf-Level Physiological Traits

    Get PDF
    With the concern of the global population to reach 9 billion by 2050, ensuring global food security is a prime challenge for the research community. One potential way to tackle this challenge is sustainable intensification; making plant phenotyping a high throughput may go a long way in this respect. Among several other plant phenotyping schemes, leaf-level plant phenotyping needs to be implemented on a large scale using existing technologies. Leaf-level chemical traits, especially macronutrients and water content are important indicators to determine crop’s health. Leaf nitrogen (N) level, is one of the critical macronutrients that carries a lot of worthwhile nutrient information for classifying the plant’s health. Hence, the non-invasive leaf’s N measurement is an innovative technique for monitoring the plant’s health. Several techniques have tried to establish a correlation between the leaf’s chlorophyll content and the N level. However, a recent study showed that the correlation between chlorophyll content and leaf’s N level is profoundly affected by environmental factors. Moreover, it is also mentioned that when the N fertilization is high, chlorophyll becomes saturated. As a result, determining the high levels of N in plants becomes difficult. Moreover, plants need an optimum level of phosphorus (P) for their healthy growth. However, the existing leaf-level P status monitoring methods are expensive, limiting their deployment for the farmers of low resourceful countries. The aim of this thesis is to develop a low-cost, portable, lightweight, multifunctional, and quick-read multispectral sensor system to sense N, P, and water in leaves non-invasively. The proposed system has been developed based on two reflectance-based multispectral sensors (visible and near-infrared (NIR)). In addition, the proposed device can capture the reflectance data at 12 different wavelengths (six for each sensor). By deploying state of the art machine learning algorithms, the spectroscopic information is modeled and validated to predict that nutrient status. A total of five experiments were conducted including four on the greenhouse-controlled environment and one in the field. Within these five, three experiments were dedicated for N sensing, one for water estimation, and one for P status determination. In the first experiment, spectral data were collected from 87 leaves of canola plants, subjected to varying levels of N fertilization. The second experiment was performed on 1008 leaves from 42 canola cultivars, which were subjected to low and high N levels, used in the field experiment. The K-Nearest Neighbors (KNN) algorithm was employed to model the reflectance data. The trained model shows an average accuracy of 88.4% on the test set for the first experiment and 79.2% for the second experiment. In the third and fourth experiments, spectral data were collected from 121 leaves for N and 186 for water experiments respectively; and Rational Quadratic Gaussian Process Regression (GPR) algorithm is applied to correlate the reflectance data with actual N and water content. By performing 5-fold cross-validation, the N estimation shows a coefficient of determination (R^2) of 63.91% for canola, 80.05% for corn, 82.29% for soybean, and 63.21% for wheat. For water content estimation, canola shows an R^2 of 18.02%, corn of 68.41%, soybean of 46.38%, and wheat of 64.58%. Finally, the fifth experiment was conducted on 267 leaf samples subjected to four levels of P treatments, and KNN exhibits the best accuracy, on the test set, of about 71.2%, 73.5%, and 67.7% for corn, soybean, and wheat, respectively. Overall, the result concludes that the proposed cost-effective sensing system can be viable in determining leaf N and P status/content. However, further investigation is needed to improve the water estimation results using the proposed device. Moreover, the utility of the device to estimate other nutrients as well as other crops has great potential for future research

    Are chlorophyll concentrations and nitrogen across the vertical canopy profile affected by elevated CO2 in mature Quercus trees?

    Get PDF
    Key message: In mature Q. robur, chlorophyll varied with season and canopy height, whilst eCO2-driven changes were consistent with Marea, highlighting key factors for consideration when scaling photosynthetic processes and canopy N-use. Nitrogen-rich chlorophyll and carotenoid pigments are important in photosynthetic functioning. Photosynthetic pigments have been found to decrease with elevated CO2 (eCO2), but few such studies have been done in aged forest trees. This study aimed to assess the effects of eCO2 (150Ă‚Â ÎŒmol mol−1 above ambient) and canopy position on chlorophyll content in mature Quercus robur (Q. robur). Over 5000 in situ chlorophyll absorbance measurements, alongside laboratory chlorophyll extractions, were collected on canopy-dominant Q. robur in the 3rd and 4th season of CO2 fumigation of a free-air CO2 enrichment (FACE) study in central England. Mass-based chlorophyll concentration (Chlmass, mg g−1) was significantly higher in the lower canopy compared to upper canopy foliage (P < 0.05). In contrast, significantly higher chlorophyll content (Chlarea, mg m−2) was observed in the upper canopy. ECO2 did not affect Chlmass but Chlarea significantly increased, attributable to increased leaf mass per unit area (Marea, g m−2). We found no effect of eCO2 on mass-based or area-based nitrogen (Nmass, mg g−1 or Narea g m−2); however, Narea significantly increased with canopy height, again attributable to Marea. The parallel relationships between Marea, Narea and Chlarea suggest the allocation of N to light harvesting is maintained with eCO2 exposure as well as in the upper canopy, and that increased photosynthetic mass may help regulate the eCO2 variation. An understanding of changes in the light-harvesting machinery with eCO2 will be useful to assess canopy processes and, at larger scales, changes in biogeochemical cycles in future climate scenarios

    Multitemporal monitoring of plant area index in the Valencia Rice District with PocketLAI

    Get PDF
    Leaf area index (LAI) is a key biophysical parameter used to determine foliage cover and crop growth in environmental studies in order to assess crop yield. Frequently, plant canopy analyzers (LAI-2000) and digital cameras for hemispherical photography (DHP) are used for indirect effective plant area index (PAIeff ) estimates. Nevertheless, these instruments are expensive and have the disadvantages of low portability and maintenance. Recently, a smartphone app called PocketLAI was presented and tested for acquiring PAIeff measurements. It was used during an entire rice season for indirect PAIeff estimations and for deriving reference high-resolution PAIeff maps. Ground PAIeff values acquired with PocketLAI, LAI-2000, and DHP were well correlated (R2 = 0.95, RMSE = 0.21 m2/m2 for Licor-2000, and R2 = 0.94, RMSE = 0.6 m2/m2 for DHP). Complementary data such as phenology and leaf chlorophyll content were acquired to complement seasonal rice plant information provided by PAIeff. High-resolution PAIeff maps, which can be used for the validation of remote sensing products, have been derived using a global transfer function (TF) made of several measuring dates and their associated satellite radiances

    Hyperspectral Modeling of Relative Water Content and Nitrogen Content in Sorghum and Maize

    Get PDF
    Sorghum and maize are two of the most important cereal grains worldwide. They are important industrially, and also serve as staple crops for millions of people across the world. With climate change, increasing frequencies of droughts, and crops being planted on more marginal land, it is important to breed sorghum and maize cultivars that are tolerant to drought and low fertility soils. However, one of the largest constraints to the breeding process is the cycle time between cultivar development and release. Early evaluation of cultivars with increased the ability to maintain water status under drought and increases nitrogen contents under nitrogen stress could be the key to decreasing breeding cycle time. New tools for non-destructive, high throughput phenotyping are needed to evaluate new cultivars. These new tools can also be used for monitoring and management of crops to improve productivity. Hyperspectral imaging holds promise as one tool to improve the speed and accuracy of predicting numerous plant traits including abiotic stress tolerance characteristics. In this thesis, hyperspectral imaging projects were designed to develop and test prediction models for relative water content (RWC) and nitrogen (N) content of sorghum and maize. The first study utilized three different genotypes of sorghum in an automated hyperspectral imaging system in greenhouses at Purdue University. From this study, models were developed for relative water content and nitrogen content using the data from all three genotypes collectively as well as the data from each genotype individually. Models developed using the spectral and morphological features obtained from the hyperspectral images are predictive of both relative water content and nitrogen content. The coefficients of determination (R2) for all graphs comparing the predicted relative water content to the reference relative water content of sorghum averaged 0.90 while the same graphs for maize averaged 0.64. The coefficients of determination for all graphs comparing the predicted nitrogen content to the reference nitrogen content of sorghum averaged 0.85 while the same graphs for maize averaged 0.61. Models built only with the spectral features for sorghum were also predictive of both relative water content and nitrogen content. The coefficients of determination for all graphs comparing the predicted relative water content to the reference relative water content of sorghum averaged 0.91 while the same graphs for nitrogen content in sorghum averaged 0.85. The nitrogen content models developed using the data from the Tx7000 genotype are highly predictive of both Tx7000 and B35 but not highly predictive of Tx623. However, models developed using the data from Tx623 are highly predictive of all three genotypes. Another important finding from this study was that the water and nitrogen signals overlap and the most predictive models are developed from data where water and nitrogen vary continuously. Models to predict one factor that do not account for variation in the other factor are not very accurate. The second experiment utilized hyperspectral imaging to characterize RWC and N content of maize. Models for RWC and N content were developed using spectral and morphological features. The models developed for maize were not as predictive as the models for sorghum but they were still predictive of RWC and N content for the models developed using all six genotypes and the models developed using the data from the individual genotypes. Models built using the four half-sibling genotypes were not more predictive than the models based on all six genotypes. The final portion of this thesis explored predictions across species using both the sorghum and maize data. We found that models developed using only sorghum were not predictive of the maize reference measurements. However, when the sorghum and maize data were combined and used to generate models, both the RWC model and the N content model were highly predictive for both reference measurements

    Early detection and quantification of verticillium wilt in olive using hyperspectral and thermal imagery over large areas

    Get PDF
    © 2015 by the authors. Automatic methods for an early detection of plant diseases (i.e., visible symptoms at early stages of disease development) using remote sensing are critical for precision crop protection. Verticillium wilt (VW) of olive caused by Verticillium dahliae can be controlled only if detected at early stages of development. Linear discriminant analysis (LDA) and support vector machine (SVM) classification methods were applied to classify V. dahliae severity using remote sensing at large scale. High-resolution thermal and hyperspectral imagery were acquired with a manned platform which flew a 3000-ha commercial olive area. LDA reached an overall accuracy of 59.0% and a Îș of 0.487 while SVM obtained a higher overall accuracy, 79.2% with a similar Îș, 0.495. However, LDA better classified trees at initial and low severity levels, reaching accuracies of 71.4 and 75.0%, respectively, in comparison with the 14.3% and 40.6% obtained by SVM. Normalized canopy temperature, chlorophyll fluorescence, structural, xanthophyll, chlorophyll, carotenoid and disease indices were found to be the best indicators for early and advanced stage infection by VW. These results demonstrate that the methods developed in other studies at orchard scale are valid for flights in large areas comprising several olive orchards differing in soil and crop management characteristics.Financial support for this research was provided by Project P08-AGR-03528 from “ConsejerĂ­a de EconomĂ­a, InnovaciĂłn y Ciencia” of Junta de AndalucĂ­a and the European Social Fund, and projects AGL-2012-37521 and AGL2012-40053-C03-01 from the Spanish “Ministerio de EconomĂ­a y Competitividad” and the European Social Fund. Rocio CalderĂłn is a recipient of research fellowship BES-2010-035511 from the Spanish “Ministerio de Ciencia e InnovaciĂłn”.We acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI).Peer Reviewe

    Spectral analysis of hybrid bermudagrass placed under various combinations of nitrogen and water availability

    Full text link
    Remote sensing technology that uses a movable ground-based system has promise for rapid, accurate and objective evaluation of turfgrass quality for instantaneous nitrogen and water application correction. Such a test has been made on hybrid bermudagrass \u27Tifway\u27 [ Cynodon dactylon (L.) Pers. x C. transvaalensis Burtt-Davy] through a 2-year field study at the Center for Urban Water Conservation in the city of North Las Vegas. Ten combinations of water and nitrogen treatments including cyclic and steady conditions were imposed on twenty experimental plots, with two replications per treatment. Treatments consisted of five N treatments: High Steady Nitrogen (HSN), Low Steady Nitrogen (LSN), High Pulse Nitrogen (HPN), Low Pulse Nitrogen (LPN), High Incremental Nitrogen (HIN), Low Incremental Nitrogen (LIN); and three water treatments based on leaching fractions: Low Leaching Fraction (LLF = -0.15), High Leaching Fraction (HLF = +0.15), Low to High Leaching Fraction (LHLF ranging from -0.25 to +0.25) all combined with N treatments in ten different combinations. Canopy spectral reflectance measurements were acquired on a biweekly basis. (Abstract shortened by UMI.)
    • 

    corecore