Polarization Optimization for the Detection of Multiple Persistent Scatterers Using SAR Tomography

The detection of multiple interfering persistent scatterers (PSs) using Synthetic Aperture Radar (SAR) tomography is an efficient tool for generating point clouds of urban areas. In this context, detection methods based upon the polarization information of SAR data are effective at increasing the number of PSs and producing high-density point clouds. This paper presents a comparative study on the effects of the polarization design of a radar antenna on further improving the probability of detecting persistent scatterers. For this purpose, we introduce an extension of the existing scattering property-based generalized likelihood ratio test (GLRT) with realistic dependence on the transmitted/received polarizations. The test is based upon polarization basis optimization by synthesizing all possible polarimetric responses of a given scatterer from its measurements on a linear orthonormal basis. Experiments on both simulated and real data show, by means of objective metrics (probability of detection, false alarm rate, and signal-to-noise ratio), that polarization waveform optimization can provide a significant performance gain in the detection of multiple scatterers compared to the existing full-polarization-based detection method. In particular, the increased density of detected PSs at the studied test sites demonstrates the main contribution of the proposed method

Urban Deformation Monitoring using Persistent Scatterer Interferometry and SAR tomography

This book focuses on remote sensing for urban deformation monitoring. In particular, it highlights how deformation monitoring in urban areas can be carried out using Persistent Scatterer Interferometry (PSI) and Synthetic Aperture Radar (SAR) Tomography (TomoSAR). Several contributions show the capabilities of Interferometric SAR (InSAR) and PSI techniques for urban deformation monitoring. Some of them show the advantages of TomoSAR in un-mixing multiple scatterers for urban mapping and monitoring. This book is dedicated to the technical and scientific community interested in urban applications. It is useful for choosing the appropriate technique and gaining an assessment of the expected performance. The book will also be useful to researchers, as it provides information on the state-of-the-art and new trends in this fiel

Performance Improvement for SAR Tomography Based on Local Plane Model

Multilook approaches have been applied in synthetic aperture radar (SAR) tomography (TomoSAR), for improving the density and regularity of persistent scatterers reconstructed from multipass SAR images in both rural and urban regions. Multilook operations assume that all scatterers in a given neighborhood are similar in height, thereby providing additional data for recovering the position and reflectivity of a single scatterer, so that a higher signal-to-noise ratio can be achieved. This is equivalent to assuming that scatterers belonging to a local neighborhood of range-azimuth cells are located on horizontal planes. The present article generalizes this approach by adopting the so-called local plane (LP) model for TomoSAR imaging in urban areas, accounting for local variations in the height of scatterers that are not negligible. Furthermore, an LP-generalized likelihood ratio test (LP-GLRT) algorithm is developed to implement the previous idea. Compared with the multilook generalized likelihood ratio test algorithm, LP-GLRT shows better performance in the case of urban structures and terrains in experiments based on both simulated data and TerraSAR-X images

Robust and Flexible Persistent Scatterer Interferometry for Long-Term and Large-Scale Displacement Monitoring

Die Persistent Scatterer Interferometrie (PSI) ist eine Methode zur Überwachung von Verschiebungen der Erdoberfläche aus dem Weltraum. Sie basiert auf der Identifizierung und Analyse von stabilen Punktstreuern (sog. Persistent Scatterer, PS) durch die Anwendung von Ansätzen der Zeitreihenanalyse auf Stapel von SAR-Interferogrammen. PS Punkte dominieren die Rückstreuung der Auflösungszellen, in denen sie sich befinden, und werden durch geringfügige Dekorrelation charakterisiert. Verschiebungen solcher PS Punkte können mit einer potenziellen Submillimetergenauigkeit überwacht werden, wenn Störquellen effektiv minimiert werden. Im Laufe der Zeit hat sich die PSI in bestimmten Anwendungen zu einer operationellen Technologie entwickelt. Es gibt jedoch immer noch herausfordernde Anwendungen für die Methode. Physische Veränderungen der Landoberfläche und Änderungen in der Aufnahmegeometrie können dazu führen, dass PS Punkte im Laufe der Zeit erscheinen oder verschwinden. Die Anzahl der kontinuierlich kohärenten PS Punkte nimmt mit zunehmender Länge der Zeitreihen ab, während die Anzahl der TPS Punkte zunimmt, die nur während eines oder mehrerer getrennter Segmente der analysierten Zeitreihe kohärent sind. Daher ist es wünschenswert, die Analyse solcher TPS Punkte in die PSI zu integrieren, um ein flexibles PSI-System zu entwickeln, das in der Lage ist mit dynamischen Veränderungen der Landoberfläche umzugehen und somit ein kontinuierliches Verschiebungsmonitoring ermöglicht. Eine weitere Herausforderung der PSI besteht darin, großflächiges Monitoring in Regionen mit komplexen atmosphärischen Bedingungen durchzuführen. Letztere führen zu hoher Unsicherheit in den Verschiebungszeitreihen bei großen Abständen zur räumlichen Referenz. Diese Arbeit befasst sich mit Modifikationen und Erweiterungen, die auf der Grund lage eines bestehenden PSI-Algorithmus realisiert wurden, um einen robusten und flexiblen PSI-Ansatz zu entwickeln, der mit den oben genannten Herausforderungen umgehen kann. Als erster Hauptbeitrag wird eine Methode präsentiert, die TPS Punkte vollständig in die PSI integriert. In Evaluierungsstudien mit echten SAR Daten wird gezeigt, dass die Integration von TPS Punkten tatsächlich die Bewältigung dynamischer Veränderungen der Landoberfläche ermöglicht und mit zunehmender Zeitreihenlänge zunehmende Relevanz für PSI-basierte Beobachtungsnetzwerke hat. Der zweite Hauptbeitrag ist die Vorstellung einer Methode zur kovarianzbasierten Referenzintegration in großflächige PSI-Anwendungen zur Schätzung von räumlich korreliertem Rauschen. Die Methode basiert auf der Abtastung des Rauschens an Referenzpixeln mit bekannten Verschiebungszeitreihen und anschließender Interpolation auf die restlichen PS Pixel unter Berücksichtigung der räumlichen Statistik des Rauschens. Es wird in einer Simulationsstudie sowie einer Studie mit realen Daten gezeigt, dass die Methode überlegene Leistung im Vergleich zu alternativen Methoden zur Reduktion von räumlich korreliertem Rauschen in Interferogrammen mittels Referenzintegration zeigt. Die entwickelte PSI-Methode wird schließlich zur Untersuchung von Landsenkung im Vietnamesischen Teil des Mekong Deltas eingesetzt, das seit einigen Jahrzehnten von Landsenkung und verschiedenen anderen Umweltproblemen betroffen ist. Die geschätzten Landsenkungsraten zeigen eine hohe Variabilität auf kurzen sowie großen räumlichen Skalen. Die höchsten Senkungsraten von bis zu 6 cm pro Jahr treten hauptsächlich in städtischen Gebieten auf. Es kann gezeigt werden, dass der größte Teil der Landsenkung ihren Ursprung im oberflächennahen Untergrund hat. Die präsentierte Methode zur Reduzierung von räumlich korreliertem Rauschen verbessert die Ergebnisse signifikant, wenn eine angemessene räumliche Verteilung von Referenzgebieten verfügbar ist. In diesem Fall wird das Rauschen effektiv reduziert und unabhängige Ergebnisse von zwei Interferogrammstapeln, die aus unterschiedlichen Orbits aufgenommen wurden, zeigen große Übereinstimmung. Die Integration von TPS Punkten führt für die analysierte Zeitreihe von sechs Jahren zu einer deutlich größeren Anzahl an identifizierten TPS als PS Punkten im gesamten Untersuchungsgebiet und verbessert damit das Beobachtungsnetzwerk erheblich. Ein spezieller Anwendungsfall der TPS Integration wird vorgestellt, der auf der Clusterung von TPS Punkten basiert, die innerhalb der analysierten Zeitreihe erschienen, um neue Konstruktionen systematisch zu identifizieren und ihre anfängliche Bewegungszeitreihen zu analysieren

Contribution to ground-based and UAV SAR systems for Earth observation

Mankind's way of life is the main driver of a planetary-scale change that is marked by the growing of human population's demand of energy, food, goods, services and information. As a result, it have emerged new ecological, economical, social and geopolitical concerns. In this scenario, SAR remote sensing is a potential tool that provides unique information about the Earth's properties and processes that can be used to solve societal challenges of local and global dimension. SARs, which are coherent systems that are able to provide high resolution images with weather independence, represent a suitable alternative for EO with diverse applications. Some examples of SAR application areas are topography (DEM generation with interferometry), agriculture (crop classification or soil moisture), or geology (monitoring surface deformation). In this framework, the encompassing objective of the present doctoral work has been part of the implementation and the subsequent evaluation of capabilities of two X-band SAR sensors. On the one hand, the RISKSAR-X radar designed to be operated at ground to monitor small-scale areas of observation and, on the other, the ARBRES-X sensor designed to be integrated into small UAVs. Despite its inherently dissimilar conception, the concurrence of both sensors has been evidenced along this manuscript. By taking advantage of the similarities between them, it has been possible to analogously assess both sensors to obtain conclusions. In this context, the common link has been the development of the polarimetric OtF operation mode of the RISKSAR-X, allowing this sensor to be operated equivalently to the ARBRES-X. Regarding the RISKSAR-X SAR sensor, several hardware contributions have been developed during part of this Ph.D. with the aim of improving the system performance. By endowing the system with the capability to operate in the fully polarimetric OtF acquisition mode, the relative long scanning time has been reduced. It is of great interest since the measured scatterers that present a short term variable reflectivity during the scanning time, such as moving vegetation, may degrade the extracted parameters from the retrieved data and the SAR image reconstruction. During this doctoral activity, it has been studied the image blurring, the decorrelation and the coherence degradation introduced by this effect. Furthermore, a new term in the differential interferometric coherence that takes into account the image blurring has been introduced. Concerning the ARBRES-X SAR system, one of the main objectives pursued during this Ph.D. has been the integration of the sensor into a small UAV MP overcoming restrictions of weight, size and aerodynamics of the platform. The use of this type of platforms is expected to open up new possibilities in airborne SAR remote sensing, since it offers much more versatility than the commonly used fixed wings UAVs. Different innovative flight strategies with this type of platforms have been assessed and some preliminary results have been obtained with the use of the ARBRES-X SAR system. During the course of the present doctoral work, much effort has been devoted to achieve the first experimental repeat-pass interfereometric results obtained with the UAV MP together with the ARBRES-X. Moreover, the sensor has been endowed with fully polarimetric capabilities by applying the improvements developed to the RISKSAR-X radar, which is another example of the duality between both systems. Finally, a vertical and a semicircular aperture have been successfully performed obtaining SLC images of the scenario, which envisages the capability of the UAV MP to perform tomographic images and complete circular apertures in the future. In conclusion, the UAV MP is a promising platform that opens new potentials for several applications, such as repeat-pass interferometry or differential tomography imaging with the realization of almost arbitrary trajectories.El mode de viure de la humanitat és el principal motor d'un canvi a escala planetària que està marcat per la creixent demanda d'energia, d'aliment, de béns, de serveis i d'informació de les poblacions humanes. Com a resultat, han sorgit noves inquietuds ecològiques, econòmiques, socials i geopolítiques. En aquest escenari, la detecció remota SAR és una eina potencial que proporciona informació única sobre les propietats i processos de la Terra que es pot utilitzar per resoldre reptes socials de dimensió local i global. Els SARs, que són sistemes coherents que poden proporcionar imatges d'alta resolució amb independència del temps, representen una alternativa adequada per a l'observació de la Terra. Alguns exemples d'àrees d'aplicació SAR són la topografia (generació de DEM amb interferometria), l'agricultura (classificació de cultius o humitat del sòl) o la geologia (monitoratge de deformació superficial). En aquest context, l'objectiu general del present doctorat ha estat part de la implementació i posterior avaluació de les capacitats de dos sensors SAR de banda X. D'una banda, el radar RISKSAR-X dissenyat per funcionar a terra i monitoritzar àrees d'observació a petita escala i, d'altra, el sensor ARBRES-X dissenyat per ser integrat en petits UAVs. Malgrat la seva concepció inherentment diferent, la concurrència d'ambdós sensors s'ha evidenciat al llarg d'aquest manuscrit. Aprofitant les similituds entre ells, s'han pogut avaluar de forma anàloga els dos sensors per obtenir conclusions. En aquest sentit, el vincle comú ha estat el desenvolupament del mode de funcionament polimètric OtF del RISKSAR-X, permetent que aquest sensor operi de forma equivalent a l'ARBRES-X. Pel que fa al sensor RISKSAR-X, s'han desenvolupat diverses contribucions hardware durant part d'aquest doctorat amb l'objectiu de millorar el rendiment del sistema. En dotar el sistema de la possibilitat d'operar en el mode d'adquisició totalment polarimètric OtF, s'ha reduït el relatiu llarg temps d'escaneig. Això és de gran interès ja que els blancs mesurats que presenten una reflectivitat variable a curt termini, com ara la vegetació en moviment, poden degradar els paràmetres extrets de les dades recuperades i la reconstrucció d'imatges SAR. Durant aquesta activitat doctoral s'ha estudiat el desenfocat de la imatge, la decorrelació i la degradació de la coherència introduïts per aquest efecte. A més, s'ha introduït un nou terme en la coherència interferomètrica diferencial que té en compte el desenfocat de la imatge. Pel que fa al sistema ARBRES-X, un dels principals objectius perseguits durant aquest doctorat ha estat la integració del sensor en un petit UAV MP superant les restriccions de pes, grandària i aerodinàmica de la plataforma. S'espera que l'ús d'aquest tipus de plataformes obri noves possibilitats en la detecció remota SAR aerotransportada, ja que ofereix molta més versatilitat que els UAV d'ales fixes habituals. S'han avaluat diferents estratègies de vol innovadores amb aquest tipus de plataformes i s'han obtingut resultats preliminars amb l'ús del sistema ARBRES-X. Durant el transcurs del present treball, s'ha dedicat molt esforç a assolir els primers resultats experimentals d'interferometria de múltiple passada obtinguts amb l'UAV MP conjuntament amb l'ARBRES-X. A més, el sensor ha estat dotat de capacitats totalment polarimètriques aplicant les millores desenvolupades al radar RISKSAR-X, el qual constitueix un altre exemple de la dualitat entre ambdós sistemes. Finalment, s'han realitzat amb èxit una apertura vertical i semicircular obtenint imatges SLC de l'escenari, el qual permet preveure la capacitat de l'UAV MP per a realitzar imatges tomogràfiques i apertures circulars completes en el futur. En conclusió, l'UAV MP és una plataforma prometedora que obre nous potencials per a diverses aplicacions, com ara la interferometria de múltiple passada o la tomografia diferencial amb la realització de trajectòries gairebé arbitràries

Contribution to ground-based and UAV SAR systems for Earth observation

Mankind's way of life is the main driver of a planetary-scale change that is marked by the growing of human population's demand of energy, food, goods, services and information. As a result, it have emerged new ecological, economical, social and geopolitical concerns. In this scenario, SAR remote sensing is a potential tool that provides unique information about the Earth's properties and processes that can be used to solve societal challenges of local and global dimension. SARs, which are coherent systems that are able to provide high resolution images with weather independence, represent a suitable alternative for EO with diverse applications. Some examples of SAR application areas are topography (DEM generation with interferometry), agriculture (crop classification or soil moisture), or geology (monitoring surface deformation). In this framework, the encompassing objective of the present doctoral work has been part of the implementation and the subsequent evaluation of capabilities of two X-band SAR sensors. On the one hand, the RISKSAR-X radar designed to be operated at ground to monitor small-scale areas of observation and, on the other, the ARBRES-X sensor designed to be integrated into small UAVs. Despite its inherently dissimilar conception, the concurrence of both sensors has been evidenced along this manuscript. By taking advantage of the similarities between them, it has been possible to analogously assess both sensors to obtain conclusions. In this context, the common link has been the development of the polarimetric OtF operation mode of the RISKSAR-X, allowing this sensor to be operated equivalently to the ARBRES-X. Regarding the RISKSAR-X SAR sensor, several hardware contributions have been developed during part of this Ph.D. with the aim of improving the system performance. By endowing the system with the capability to operate in the fully polarimetric OtF acquisition mode, the relative long scanning time has been reduced. It is of great interest since the measured scatterers that present a short term variable reflectivity during the scanning time, such as moving vegetation, may degrade the extracted parameters from the retrieved data and the SAR image reconstruction. During this doctoral activity, it has been studied the image blurring, the decorrelation and the coherence degradation introduced by this effect. Furthermore, a new term in the differential interferometric coherence that takes into account the image blurring has been introduced. Concerning the ARBRES-X SAR system, one of the main objectives pursued during this Ph.D. has been the integration of the sensor into a small UAV MP overcoming restrictions of weight, size and aerodynamics of the platform. The use of this type of platforms is expected to open up new possibilities in airborne SAR remote sensing, since it offers much more versatility than the commonly used fixed wings UAVs. Different innovative flight strategies with this type of platforms have been assessed and some preliminary results have been obtained with the use of the ARBRES-X SAR system. During the course of the present doctoral work, much effort has been devoted to achieve the first experimental repeat-pass interfereometric results obtained with the UAV MP together with the ARBRES-X. Moreover, the sensor has been endowed with fully polarimetric capabilities by applying the improvements developed to the RISKSAR-X radar, which is another example of the duality between both systems. Finally, a vertical and a semicircular aperture have been successfully performed obtaining SLC images of the scenario, which envisages the capability of the UAV MP to perform tomographic images and complete circular apertures in the future. In conclusion, the UAV MP is a promising platform that opens new potentials for several applications, such as repeat-pass interferometry or differential tomography imaging with the realization of almost arbitrary trajectories.El mode de viure de la humanitat és el principal motor d'un canvi a escala planetària que està marcat per la creixent demanda d'energia, d'aliment, de béns, de serveis i d'informació de les poblacions humanes. Com a resultat, han sorgit noves inquietuds ecològiques, econòmiques, socials i geopolítiques. En aquest escenari, la detecció remota SAR és una eina potencial que proporciona informació única sobre les propietats i processos de la Terra que es pot utilitzar per resoldre reptes socials de dimensió local i global. Els SARs, que són sistemes coherents que poden proporcionar imatges d'alta resolució amb independència del temps, representen una alternativa adequada per a l'observació de la Terra. Alguns exemples d'àrees d'aplicació SAR són la topografia (generació de DEM amb interferometria), l'agricultura (classificació de cultius o humitat del sòl) o la geologia (monitoratge de deformació superficial). En aquest context, l'objectiu general del present doctorat ha estat part de la implementació i posterior avaluació de les capacitats de dos sensors SAR de banda X. D'una banda, el radar RISKSAR-X dissenyat per funcionar a terra i monitoritzar àrees d'observació a petita escala i, d'altra, el sensor ARBRES-X dissenyat per ser integrat en petits UAVs. Malgrat la seva concepció inherentment diferent, la concurrència d'ambdós sensors s'ha evidenciat al llarg d'aquest manuscrit. Aprofitant les similituds entre ells, s'han pogut avaluar de forma anàloga els dos sensors per obtenir conclusions. En aquest sentit, el vincle comú ha estat el desenvolupament del mode de funcionament polimètric OtF del RISKSAR-X, permetent que aquest sensor operi de forma equivalent a l'ARBRES-X. Pel que fa al sensor RISKSAR-X, s'han desenvolupat diverses contribucions hardware durant part d'aquest doctorat amb l'objectiu de millorar el rendiment del sistema. En dotar el sistema de la possibilitat d'operar en el mode d'adquisició totalment polarimètric OtF, s'ha reduït el relatiu llarg temps d'escaneig. Això és de gran interès ja que els blancs mesurats que presenten una reflectivitat variable a curt termini, com ara la vegetació en moviment, poden degradar els paràmetres extrets de les dades recuperades i la reconstrucció d'imatges SAR. Durant aquesta activitat doctoral s'ha estudiat el desenfocat de la imatge, la decorrelació i la degradació de la coherència introduïts per aquest efecte. A més, s'ha introduït un nou terme en la coherència interferomètrica diferencial que té en compte el desenfocat de la imatge. Pel que fa al sistema ARBRES-X, un dels principals objectius perseguits durant aquest doctorat ha estat la integració del sensor en un petit UAV MP superant les restriccions de pes, grandària i aerodinàmica de la plataforma. S'espera que l'ús d'aquest tipus de plataformes obri noves possibilitats en la detecció remota SAR aerotransportada, ja que ofereix molta més versatilitat que els UAV d'ales fixes habituals. S'han avaluat diferents estratègies de vol innovadores amb aquest tipus de plataformes i s'han obtingut resultats preliminars amb l'ús del sistema ARBRES-X. Durant el transcurs del present treball, s'ha dedicat molt esforç a assolir els primers resultats experimentals d'interferometria de múltiple passada obtinguts amb l'UAV MP conjuntament amb l'ARBRES-X. A més, el sensor ha estat dotat de capacitats totalment polarimètriques aplicant les millores desenvolupades al radar RISKSAR-X, el qual constitueix un altre exemple de la dualitat entre ambdós sistemes. Finalment, s'han realitzat amb èxit una apertura vertical i semicircular obtenint imatges SLC de l'escenari, el qual permet preveure la capacitat de l'UAV MP per a realitzar imatges tomogràfiques i apertures circulars completes en el futur. En conclusió, l'UAV MP és una plataforma prometedora que obre nous potencials per a diverses aplicacions, com ara la interferometria de múltiple passada o la tomografia diferencial amb la realització de trajectòries gairebé arbitràries.Postprint (published version

Study of the speckle noise effects over the eigen decomposition of polarimetric SAR data: a review

This paper is focused on considering the effects of speckle noise on the eigen decomposition of the co- herency matrix. Based on a perturbation analysis of the matrix, it is possible to obtain an analytical expression for the mean value of the eigenvalues and the eigenvectors, as well as for the Entropy, the Anisotroopy and the dif- ferent a angles. The analytical expressions are compared against simulated polarimetric SAR data, demonstrating the correctness of the different expressions.Peer ReviewedPostprint (published version

Spatial and spatiotemporal variation in metapopulation structure affects population dynamics in a passively dispersing arthropod

The spatial and temporal variation in the availability of suitable habitat within metapopulations determines colonization-extinction events, regulates local population sizes and eventually affects local population and metapopulation stability. Insights into the impact of such a spatiotemporal variation on the local population and metapopulation dynamics are principally derived from classical metapopulation theory and have not been experimentally validated. By manipulating spatial structure in artificial metapopulations of the spider mite Tetranychus urticae, we test to which degree spatial (mainland-island metapopulations) and spatiotemporal variation (classical metapopulations) in habitat availability affects the dynamics of the metapopulations relative to systems where habitat is constantly available in time and space (patchy metapopulations). Our experiment demonstrates that (i) spatial variation in habitat availability decreases variance in metapopulation size and decreases density-dependent dispersal at the metapopulation level, while (ii) spatiotemporal variation in habitat availability increases patch extinction rates, decreases local population and metapopulation sizes and decreases density dependence in population growth rates. We found dispersal to be negatively density dependent and overall low in the spatial variable mainland-island metapopulation. This demographic variation subsequently impacts local and regional population dynamics and determines patterns of metapopulation stability. Both local and metapopulation-level variabilities are minimized in mainland-island metapopulations relative to classical and patchy ones

Aggregation-Induced Emission (AIE), Life and Health

Light has profoundly impacted modern medicine and healthcare, with numerous luminescent agents and imaging techniques currently being used to assess health and treat diseases. As an emerging concept in luminescence, aggregation-induced emission (AIE) has shown great potential in biological applications due to its advantages in terms of brightness, biocompatibility, photostability, and positive correlation with concentration. This review provides a comprehensive summary of AIE luminogens applied in imaging of biological structure and dynamic physiological processes, disease diagnosis and treatment, and detection and monitoring of specific analytes, followed by representative works. Discussions on critical issues and perspectives on future directions are also included. This review aims to stimulate the interest of researchers from different fields, including chemistry, biology, materials science, medicine, etc., thus promoting the development of AIE in the fields of life and health

Functional Gold Nanoparticles for Biomedical Applications

Abstract Subjects of the present dissertation are the synthesis, the functionalization and the characterization of colloidal gold nanoparticles. The employed nanoparticles consist of an inorganic Au core of approximately 5 nm diameter, which is stabilized by hydrophobic surface molecules. To transfer the nanoparticles to aqueous environments (an indispensable necessity for biomedical applications) they are coated with an amphiphilic polymer, which generates water solubility and moreover gives the ability for further functionalization. The physico-chemical properties of such nanoparticles are verified within different purposes: First, several fundamental intrinsic surface properties are quantified, including the establishment of pH titration as characterization tool. It is found that the carboxylic groups, responsible for the colloidal stabilization, partly have different properties (like their pKa) compared to free standing carboxylic acids. These findings are crucial for the colloidal stabilization of nanoparticles as well as for their further functionalization. Secondly, two species of fluorescently labeled nanoparticles, which differed in first order only in the net surface charge, are employed to study charge dependent interaction of nanoparticles with biological systems, including proteins as well as living cells. The main finding is, that a so called protein corona forms around nanoparticles, what has far-reaching impacts on cell internalization abilities. Moreover it is found that positively charged nanoparticles show a higher cell association as well as a higher toxicity. Thirdly, nanoparticles are modified towards sensing applications by surface functionalization with ion sensitive dyes. Positively charged nanoparticles are modified with a Cl- sensitive dye and negatively charged nanoparticles are modified with a Zn2+ sensitive dye. The goals of the dissertation can be synoptically depicted as: 1) Extension of the existing techniques for nanoparticle functionalization, particularly regarding new types of functional polymers. 2) A fundamental and comprehensive characterization of nanoparticles ranging from the verification of intrinsic, physico-chemical properties to biomedical applications