DR9.3 Final report of the JRRM and ASM activities

Deliverable del projecte europeu NEWCOM++This deliverable provides the final report with the summary of the activities carried out in NEWCOM++ WPR9, with a particular focus on those obtained during the last year. They address on the one hand RRM and JRRM strategies in heterogeneous scenarios and, on the other hand, spectrum management and opportunistic spectrum access to achieve an efficient spectrum usage. Main outcomes of the workpackage as well as integration indicators are also summarised.Postprint (published version

Ergodic rate for fading interference channels with proper and improper Gaussian signaling

This paper studies the performance of improper Gaussian signaling (IGS) over a 2-user Rayleigh single-input single-output (SISO) interference channel, treating interference as noise. We assume that the receivers have perfect channel state information (CSI), while the transmitters have access to only statistical CSI. Under these assumptions, we consider a signaling scheme, which we refer to as proper/improper Gaussian signaling or PGS/IGS, where at most one user may employ IGS. For the Rayleigh fading channel model, we characterize the statistical distribution of the signal-to-interference-plus-noise ratio at each receiver and derive closed-form expressions for the ergodic rates. By adapting the powers, we characterize the Pareto boundary of the ergodic rate region for the 2-user fading IC. The ergodic transmission rates can be attained using fixed-rate codebooks and no optimization is involved. Our results show that, in the moderate and strong interference regimes, the proposed PGS/IGS scheme improves the performance with respect to the PGS scheme. Additionally, we numerically compute the ergodic rate region of the full IGS scheme when both users can employ IGS and their transmission parameters are optimized by an exhaustive search. Our results suggest that most of the Pareto optimal points for the 2-user fading IC channel are attained when either both users transmit PGS or when one transmits PGS and the other transmits maximally improper Gaussian signals and time sharing is allowed.The work of M. Soleymani, C. Lameiro and P. J. Schreier was supported by the German Research Foundation (DFG) under grants LA 4107/1-1, SCHR 1384/7-1 and SCHR 1384/8-1. The work of I. Santamaria was supported by MINECO of Spain and AEI/FEDER funds of the E.U., under grant TEC2016-75067-C4-4-R (CARMEN)

Structural Characterization of Acidic M17 Leucine Aminopeptidases from the TriTryps and Evaluation of Their Role in Nutrient Starvation in Trypanosoma brucei

Leucine aminopeptidase (LAP) is found in all kingdoms of life and catalyzes the metal-dependent hydrolysis of the N-terminal amino acid residue of peptide or amino acyl substrates. LAPs have been shown to participate in the N-terminal processing of certain proteins in mammalian cells and in homologous recombination and transcription regulation in bacteria, while in parasites, they are involved in host cell invasion and provision of essential amino acids for growth. The enzyme is essential for survival in Plasmodium falciparum, where its drug target potential has been suggested. We report here the X-ray structures of three kinetoplastid acidic LAPs (LAP-As from Trypanosoma brucei, Trypanosoma cruzi, and Leishmania major) which were solved in the metal-free and unliganded forms, as well as in a number of ligand complexes, providing insight into ligand binding, metal ion requirements, and oligomeric state. In addition, we analyzed mutant cells defective in LAP-A in Trypanosoma brucei, strongly suggesting that the enzyme is not required for the growth of this parasite either in vitro or in vivo. In procyclic cells, LAP-A was equally distributed throughout the cytoplasm, yet upon starvation, it relocalizes in particles that concentrate in the perinuclear region. Overexpression of the enzyme conferred a growth advantage when parasites were grown in leucine-deficient medium. Overall, the results suggest that in T. brucei, LAP-A may participate in protein degradation associated with nutrient depletion. IMPORTANCE Leucine aminopeptidases (LAPs) catalyze the hydrolysis of the N-terminal amino acid of peptides and are considered potential drug targets. They are involved in multiple functions ranging from host cell invasion and provision of essential amino acids to site-specific homologous recombination and transcription regulation. In kinetoplastid parasites, there are at least three distinct LAPs. The availability of the crystal structures provides important information for drug design. Here we report the structure of the acidic LAPs from three kinetoplastids in complex with different inhibitors and explore their role in Trypanosoma brucei survival under various nutrient conditions. Importantly, the acidic LAP is dispensable for growth both in vitro and in vivo, an observation that questions its use as a specific drug target. While LAP-A is not essential, leucine depletion and subcellular localization studies performed under starvation conditions suggest a possible function of LAP-A in the response to nutrient restriction

Arabidopsis thaliana dehydroascorbate reductase 2 : conformational flexibility during catalysis

Dehydroascorbate reductase (DHAR) catalyzes the glutathione (GSH)-dependent reduction of dehydroascorbate and plays a direct role in regenerating ascorbic acid, an essential plant antioxidant vital for defense against oxidative stress. DHAR enzymes bear close structural homology to the glutathione transferase (GST) superfamily of enzymes and contain the same active site motif, but most GSTs do not exhibit DHAR activity. The presence of a cysteine at the active site is essential for the catalytic functioning of DHAR, as mutation of this cysteine abolishes the activity. Here we present the crystal structure of DHAR2 from Arabidopsis thaliana with GSH bound to the catalytic cysteine. This structure reveals localized conformational differences around the active site which distinguishes the GSH-bound DHAR2 structure from that of DHAR1. We also unraveled the enzymatic step in which DHAR releases oxidized glutathione (GSSG). To consolidate our structural and kinetic findings, we investigated potential conformational flexibility in DHAR2 by normal mode analysis and found that subdomain mobility could be linked to GSH binding or GSSG release

Proceedings of a workshop on Lunar Volcanic Glasses: Scientific and Resource Potential

This workshop on lunar mare volcanism was the first since 1975 to deal with the major scientific advances that have occurred in this general subject, and the first ever to deal specifically with volcanic glasses. Lunar volcanic glasses are increasingly being recognized as the best geochemical and petrologic probes into the lunar mantle. Lunar volcanic glasses, of which 25 compositional varieties are presently known, appear to represent primary magmas that were produced by partial melting of differentiated mantle source regions at depths of perhaps 400 to 500 km. These high-magnesian picritic magmas were erupted onto the lunar surface in fire fountains associated with the release of indigenous lunar volatiles. The cosmic significance of this volatile component, in an otherwise depleted Moon, remains a lingering puzzle. The resource potential, if any, of the surface-correlated volatile sublimates on the volcanic glass spherules had not been systematically addressed prior to this workshop

Structure of the CaMKIIδ/Calmodulin Complex Reveals the Molecular Mechanism of CaMKII Kinase Activation

Structural and biophysical studies reveal how CaMKII kinases, which are important for cellular learning and memory, are switched on by binding of Ca2+/calmodulin