Detection processes for digital satellite modems

The aim of this study is to devise detectors for digital satellite modems, that have tolerances to additive white Gaussian noise which are as close as possible to that for optimal detection, at a fraction of the equipment complexity required for optimal detection. Computer simulation tests and theoretical analyses are used to compare the proposed detectors. [Continues.

Overview of high-speed TDM-PON beyond 50 Gbps per wavelength using digital signal processing [Invited Tutorial]

The recent evolution of passive optical network standards and related research activities for physical layer solutions that achieve bit rates well above 10 Gbps per wavelength (lambda) is discussed. We show that the advancement toward 50, 100, and 200 Gbps/lambda will certainly require a strong introduction of advanced digital signal processing (DSP) technologies for linear, and maybe nonlinear, equalization and for forward error correction. We start by reviewing in detail the current standardization activities in the International Telecommunication Union and the Institute of Electrical and Electronics Engineers, and then we present a comparison of the DSP approaches for traditional direct detection solutions and for future coherent detection approaches. (c) 2022 Optica Publishing Grou

Functional impact of inactivating mutations in epigenetic regulators in cancer

Cancer evolution is driven by selection acting on genetic and epigenetic diversity to promote the propagation of the fittest subpopulations. This phenomenon is shaped by the tumor microenvironment which is often characterized by stressful conditions. Epigenetic regulators are frequently mutated during the later stages of tumorigenesis, but the functional impact of their inactivation is poorly understood. In this thesis, I hypothesize that the disruption of the epigenetic regulatory network increases cell fitness in unfavorable environments and thus is selected over time. Through large-scale fitness assays in various cancer models, I demonstrate that epigenetic deregulation leads to a widespread stress-specific survival advantage. This effect is mediated by mutations in all layers of epigenetic regulation, is shared across different stress conditions and is cancer type independent. Then, I explore various cellular mechanisms that can underlie this stress-specific fitness advantage. Genetic diversity, transcriptional heterogeneity or phenotypic plasticity cannot explain the increased survival under stress, as revealed by a combination of reversible epigenetic inhibition, live-cell imaging and single-cell transcriptomics. On the contrary, epigenetically deregulated cells remain phenotypically inert (less responsive) under stress. Transcriptional profiling of cancer populations in hostile conditions, revealed significant alterations in fitness and growth-related signatures. Disruption of the epigenetic machinery results in a defective stress response, thus decreasing the probability of such cells to surpass a stressed threshold and ultimately die. This defective transcriptional rewiring underpins the inert phenotype that emerges upon epigenetic deregulation. Collectively, by investigating the effect of inactivating mutations in epigenetic regulators on cell fitness under environmental stress, I propose that phenotypic inertia is the favorable cellular trait that is selected over time. My findings provide a potential explanation for the widespread subclonal mutations affecting epigenetic regulators and have significant implications for cancer evolution.Open Acces

Design of adaptive analog filters for magnetic front-end read channels

Esta tese estuda o projecto e o comportamento de filtros em tempo contínuo de muito-alta-frequência. A motivação deste trabalho foi a investigação de soluções de filtragem para canais de leitura em sistemas de gravação e reprodução de dados em suporte magnético, com custos e consumo (tamanho total inferior a 1 mm2 e consumo inferior a 1mW/polo), inferiores aos circuitos existentes. Nesse sentido, tal como foi feito neste trabalho, o rápido desenvolvimento das tecnologias de microelectrónica suscitou esforços muito significativos a nível mundial com o objectivo de se investigarem novas técnicas de realização de filtros em circuito integrado monolítico, especialmente em tecnologia CMOS (Complementary Metal Oxide Semiconductor). Apresenta-se um estudo comparativo a diversos níveis hierárquicos do projecto, que conduziu à realização e caracterização de soluções com as características desejadas. Num primeiro nível, este estudo aborda a questão conceptual da gravação e transmissão de sinal bem como a escolha de bons modelos matemáticos para o tratamento da informação e a minimização de erro inerente às aproximações na conformidade aos princípios físicos dos dispositivos caracterizados. O trabalho principal da tese é focado nos níveis hierárquicos da arquitectura do canal de leitura e da realização em circuito integrado do seu bloco principal – o bloco de filtragem. Ao nível da arquitectura do canal de leitura, apresenta-se um estudo alargado sobre as metodologias existentes de adaptação de sinal e recuperação de dados em suporte magnético. Este desígnio aparece no âmbito da proposta de uma solução de baixo custo, baixo consumo, baixa tensão de alimentação e baixa complexidade, alicerçada em tecnologia digital CMOS, para a realização de um sistema DFE (Decision Feedback Equalization) com base na igualização de sinal utilizando filtros integrados analógicos em tempo contínuo. Ao nível do projecto de realização do bloco de filtragem e das técnicas de implementação de filtros e dos seus blocos constituintes em circuito integrado, concluiu-se que a técnica baseada em circuitos de transcondutância e condensadores, também conhecida como filtros gm-C (ou transcondutância-C), é a mais adequada para a realização de filtros adaptativos em muito-alta-frequência. Definiram-se neste nível hierárquico mais baixo, dois subníveis de aprofundamento do estudo no âmbito desta tese, nomeadamente: a pesquisa e análise de estruturas ideais no projecto de filtros recorrendo a representações no espaço de estados; e, o estudo de técnicas de realização em tecnologia digital CMOS de circuitos de transcondutância para a implementação de filtros integrados analógicos em tempo contínuo. Na sequência deste estudo, apresentam-se e comparam-se duas estruturas de filtros no espaço de estados, correspondentes a duas soluções alternativas para a realização de um igualador adaptativo realizado por um filtro contínuo passa-tudo de terceira ordem, para utilização num canal de leitura de dados em suporte magnético. Como parte constituinte destes filtros, apresenta-se uma técnica de realização de circuitos de transcondutância, e de realização de condensadores lineares usando matrizes de transístores MOSFET para processamento de sinal em muito-alta-frequência realizada em circuito integrado usando tecnologia digital CMOS submicrométrica. Apresentam-se métodos de adaptação automática capazes de compensar os erros face aos valores nominais dos componentes, devidos às tolerâncias inerentes ao processo de fabrico, para os quais apresentamos os resultados de simulação e de medição experimental obtidos. Na sequência deste estudo, resultou igualmente a apresentação de um circuito passível de constituir uma solução para o controlo de posicionamento da cabeça de leitura em sistemas de gravação/reprodução de dados em suporte magnético. O bloco proposto é um filtro adaptativo de primeira ordem, com base nos mesmos circuitos de transcondutância e técnicas de igualação propostos e utilizados na implementação do filtro adaptativo de igualação do canal de leitura. Este bloco de filtragem foi projectado e incluído num circuito integrado (Jaguar) de controlo de posicionamento da cabeça de leitura realizado para a empresa ATMEL em Colorado Springs, e incluído num produto comercial em parceria com uma empresa escocesa utilizado em discos rígidos amovíveis.This thesis studies the design and behavior of continuous-time very-high-frequency filters. The motivation of this work was the search for filtering solutions for the readchannel in recording and reproduction of data on magnetic media systems, with costs and consumption (total size less than 1 mm2 and consumption under 1mW/pole), lower than the available circuits. Accordingly, as was done in this work, the rapid development of microelectronics technology raised very significant efforts worldwide in order to investigate new techniques for implementing such filters in monolithic integrated circuit, especially in CMOS technology (Complementary Metal Oxide Semiconductor). We present a comparative study on different hierarchical levels of the project, which led to the realization and characterization of solutions with the desired characteristics. In the first level, this study addresses the conceptual question of recording and transmission of signal and the choice of good mathematical models for the processing of information and minimization of error inherent in the approaches and in accordance with the principles of the characterized physical devices. The main work of this thesis is focused on the hierarchical levels of the architecture of the read channel and the integrated circuit implementation of its main block - the filtering block. At the architecture level of the read channel this work presents a comprehensive study on existing methodologies of adaptation and signal recovery of data on magnetic media. This project appears in the sequence of the proposed solution for a lowcost, low consumption, low voltage, low complexity, using CMOS digital technology for the performance of a DFE (Decision Feedback Equalization) based on the equalization of the signal using integrated analog filters in continuous time. At the project level of implementation of the filtering block and techniques for implementing filters and its building components, it was concluded that the technique based on transconductance circuits and capacitors, also known as gm-C filters is the most appropriate for the implementation of very-high-frequency adaptive filters. We defined in this lower level, two sub-levels of depth study for this thesis, namely: research and analysis of optimal structures for the design of state-space filters, and the study of techniques for the design of transconductance cells in digital CMOS circuits for the implementation of continuous time integrated analog filters. Following this study, we present and compare two filtering structures operating in the space of states, corresponding to two alternatives for achieving a realization of an adaptive equalizer by the use of a continuous-time third order allpass filter, as part of a read-channel for magnetic media devices. As a constituent part of these filters, we present a technique for the realization of transconductance circuits and for the implementation of linear capacitors using arrays of MOSFET transistors for signal processing in very-high-frequency integrated circuits using sub-micrometric CMOS technology. We present methods capable of automatic adjustment and compensation for deviation errors in respect to the nominal values of the components inherent to the tolerances of the fabrication process, for which we present the simulation and experimental measurement results obtained. Also as a result of this study, is the presentation of a circuit that provides a solution for the control of the head positioning on recording/playback systems of data on magnetic media. The proposed block is an adaptive first-order filter, based on the same transconductance circuits and equalization techniques proposed and used in the implementation of the adaptive filter for the equalization of the read channel. This filter was designed and included in an integrated circuit (Jaguar) used to control the positioning of the read-head done for ATMEL company in Colorado Springs, and part of a commercial product used in removable hard drives fabricated in partnership with a Scottish company

Application of advanced on-board processing concepts to future satellite communications systems: Bibliography

Abstracts are presented of a literature survey of reports concerning the application of signal processing concepts. Approximately 300 references are included

Subthalamic nucleus activity dynamics and limb movement prediction in Parkinson’s disease

Whilst exaggerated bursts of beta frequency band oscillatory synchronization in the subthalamic nucleus have been associated with motor impairment in Parkinson’s disease, a plausible mechanism linking the two phenomena has been lacking. Here we test the hypothesis that increased synchronization denoted by beta bursting might compromise information coding capacity in basal ganglia networks. To this end we recorded local field potential activity in the subthalamic nucleus of 18 patients with Parkinson’s disease as they executed cued upper and lower limb movements. We used the accuracy of local field potential-based classification of the limb to be moved on each trial as an index of the information held by the system with respect to intended action. Machine learning using the naïve Bayes conditional probability model was used for classification. Local field potential dynamics allowed accurate prediction of intended movements well ahead of their execution, with an area under the receiver operator characteristic curve of 0.80 ± 0.04 before imperative cues when the demanded action was known ahead of time. The presence of bursts of local field potential activity in the alpha, and even more so, in the beta frequency band significantly compromised the prediction of the limb to be moved. We conclude that low frequency bursts, particularly those in the beta band, restrict the capacity of the basal ganglia system to encode physiologically relevant information about intended actions. The current findings are also important as they suggest that local subthalamic activity may potentially be decoded to enable effector selection, in addition to force control in restorative brain-machine interface applications

RNA Polymerase II cluster dynamics predict mRNA output in living cells

Protein clustering is a hallmark of genome regulation in mammalian cells. However, the dynamic molecular processes involved make it difficult to correlate clustering with functional consequences in vivo. We developed a live-cell super-resolution approach to uncover the correlation between mRNA synthesis and the dynamics of RNA Polymerase II (Pol II) clusters at a gene locus. For endogenous β-actin genes in mouse embryonic fibroblasts, we observe that short-lived (~8 s) Pol II clusters correlate with basal mRNA output. During serum stimulation, a stereotyped increase in Pol II cluster lifetime correlates with a proportionate increase in the number of mRNAs synthesized. Our findings suggest that transient clustering of Pol II may constitute a pre-transcriptional regulatory event that predictably modulates nascent mRNA output.National Cancer Institute (U.S.) (DP2CA195769

Extracellular Superoxide Dismutase Protects Histoplasma Yeast Cells from Host-Derived Oxidative Stress

In order to establish infections within the mammalian host, pathogens must protect themselves against toxic reactive oxygen species produced by phagocytes of the immune system. The fungal pathogen Histoplasma capsulatum infects both neutrophils and macrophages but the mechanisms enabling Histoplasma yeasts to survive in these phagocytes have not been fully elucidated. We show that Histoplasma yeasts produce a superoxide dismutase (Sod3) and direct it to the extracellular environment via N-terminal and C-terminal signals which promote its secretion and association with the yeast cell surface. This localization permits Sod3 to protect yeasts specifically from exogenous superoxide whereas amelioration of endogenous reactive oxygen depends on intracellular dismutases such as Sod1. While infection of resting macrophages by Histoplasma does not stimulate the phagocyte oxidative burst, interaction with polymorphonuclear leukocytes (PMNs) and cytokine-activated macrophages triggers production of reactive oxygen species (ROS). Histoplasma yeasts producing Sod3 survive co-incubation with these phagocytes but yeasts lacking Sod3 are rapidly eliminated through oxidative killing similar to the effect of phagocytes on Candida albicans yeasts. The protection provided by Sod3 against host-derived ROS extends in vivo. Without Sod3, Histoplasma yeasts are attenuated in their ability to establish respiratory infections and are rapidly cleared with the onset of adaptive immunity. The virulence of Sod3-deficient yeasts is restored in murine hosts unable to produce superoxide due to loss of the NADPH-oxidase function. These results demonstrate that phagocyte-produced ROS contributes to the immune response to Histoplasma and that Sod3 facilitates Histoplasma pathogenesis by detoxifying host-derived reactive oxygen thereby enabling Histoplasma survival