Amplifying LGBTQ Voices in Social Work

This banded dissertation is focused on amplifying LGBTQ voices in social work education and practice through an exploration of language, policies, standards, and practices used in social work education. Using a historical lens, feminist, queer, and critical theories were used to examine issues of power, voice, context, and social justice. The first product is a conceptual paper that examines the history of the language used in social work education related to how we think and talk about diversity. This examination includes a critique of the use of the term difference and the othering impact it can have on LGBTQ individuals and communities, deeming LGBTQ people as inherently different, deviant, or abnormal based on their sexual orientation, gender identity, and/or gender expression. The second product is a historical content analysis examining the conversation at the Council on Social Work Education regarding LGBTQ related issues from 1980-2015. This analysis expands on the literature and highlights tensions as well as advocacy efforts related to a variety of issues, most notably a recurring debate regarding policies and ethical standards that polarized religious freedom and LGBTQ rights. The third product is a presentation on product one, the conceptual paper exploring the language used to understand and talk about diversity in social work education over time. The presentation included the author’s recommendation to remove the term difference from the social work education competency language in an effort to move away from a binary and dominate subordinate language structure that can other people. The LGBTQ community served as an example group to illustrate the impact in practice. This banded dissertation is aimed at amplifying LGBTQ voices through exploration and documentation of issues that impact LTBTQ people in social work education and practice. This work provides several points of opportunity for curricular infusion related to social work education history, diversity, social justice, and ethics as well as opportunities for additional research that could further amplify LGBTQ voices in social work education and practice, such as conducting individual interviews and developing a case study

Amplifying LGBTQ Voices in Social Work

This banded dissertation is focused on amplifying LGBTQ voices in social work education and practice through an exploration of language, policies, standards, and practices used in social work education. Using a historical lens, feminist, queer, and critical theories were used to examine issues of power, voice, context, and social justice. The first product is a conceptual paper that examines the history of the language used in social work education related to how we think and talk about diversity. This examination includes a critique of the use of the term difference and the othering impact it can have on LGBTQ individuals and communities, deeming LGBTQ people as inherently different, deviant, or abnormal based on their sexual orientation, gender identity, and/or gender expression. The second product is a historical content analysis examining the conversation at the Council on Social Work Education regarding LGBTQ related issues from 1980-2015. This analysis expands on the literature and highlights tensions as well as advocacy efforts related to a variety of issues, most notably a recurring debate regarding policies and ethical standards that polarized religious freedom and LGBTQ rights. The third product is a presentation on product one, the conceptual paper exploring the language used to understand and talk about diversity in social work education over time. The presentation included the author’s recommendation to remove the term difference from the social work education competency language in an effort to move away from a binary and dominate subordinate language structure that can other people. The LGBTQ community served as an example group to illustrate the impact in practice. This banded dissertation is aimed at amplifying LGBTQ voices through exploration and documentation of issues that impact LTBTQ people in social work education and practice. This work provides several points of opportunity for curricular infusion related to social work education history, diversity, social justice, and ethics as well as opportunities for additional research that could further amplify LGBTQ voices in social work education and practice, such as conducting individual interviews and developing a case study

Optimizing identification of clinically relevant gram-positive organisms by use of the bruker biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry system

Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) can be used as a method for the rapid identification of microorganisms. This study evaluated the Bruker Biotyper (MALDI-TOF MS) system for the identification of clinically relevant Gram-positive organisms. We tested 239 aerobic Gram-positive organisms isolated from clinical specimens. We evaluated 4 direct-smear methods, including “heavy” (H) and “light” (L) smears, with and without a 1-μl direct formic acid (FA) overlay. The quality measure assigned to a MALDI-TOF MS identification is a numerical value or “score.” We found that a heavy smear with a formic acid overlay (H+FA) produced optimal MALDI-TOF MS identification scores and the highest percentage of correctly identified organisms. Using a score of ≥2.0, we identified 183 of the 239 isolates (76.6%) to the genus level, and of the 181 isolates resolved to the species level, 141 isolates (77.9%) were correctly identified. To maximize the number of correct identifications while minimizing misidentifications, the data were analyzed using a score of ≥1.7 for genus- and species-level identification. Using this score, 220 of the 239 isolates (92.1%) were identified to the genus level, and of the 181 isolates resolved to the species level, 167 isolates (92.2%) could be assigned an accurate species identification. We also evaluated a subset of isolates for preanalytic factors that might influence MALDI-TOF MS identification. Frequent subcultures increased the number of unidentified isolates. Incubation temperatures and subcultures of the media did not alter the rate of identification. These data define the ideal bacterial preparation, identification score, and medium conditions for optimal identification of Gram-positive bacteria by use of MALDI-TOF MS

Discovery of the infrared counterpart of CXOU J174437.3-323222 in the field of IGR J17448-3232: a blazar candidate viewed through the Galactic centre?

We present our near infrared ESO-NTT Ks band observations of the field of IGR J17448-3232 which show no extended emission consistent with the SNR but in which we identify a new counterpart, also visible in Spitzer images up to 24 {\mu}m, at the position of the X-ray point source, CXOUJ174437.3-323222. Multi-wavelength spectral modelling shows that the data are consistent with a reddened and absorbed single power law over five orders of magnitude in frequency. This implies non-thermal, possibly synchrotron emission that renders the previous identification of this source as a possible pulsar, and its association to the SNR, unlikely; we instead propose that the emission may be due to a blazar viewed through the plane of the Galaxy.Comment: MNRAS Letters (5 pages, 3 figures); Table 1 corrected in this versio

Understanding of sub-band gap absorption of femtosecond-laser sulfur hyperdoped silicon using synchrotron-based techniques

[[abstract]]The correlation between sub-band gap absorption and the chemical states and electronic and atomic structures of S-hyperdoped Si have been extensively studied, using synchrotron-based x-ray photoelectron spectroscopy (XPS), x-ray absorption near-edge spectroscopy (XANES), extended x-ray absorption fine structure (EXAFS), valence-band photoemission spectroscopy (VB-PES) and first-principles calculation. S 2p XPS spectra reveal that the S-hyperdoped Si with the greatest (~87%) sub-band gap absorption contains the highest concentration of S2− (monosulfide) species. Annealing S-hyperdoped Si reduces the sub-band gap absorptance and the concentration of S2− species, but significantly increases the concentration of larger S clusters [polysulfides (Sn2−, n > 2)]. The Si K-edge XANES spectra show that S hyperdoping in Si increases (decreased) the occupied (unoccupied) electronic density of states at/above the conduction-band-minimum. VB-PES spectra evidently reveal that the S-dopants not only form an impurity band deep within the band gap, giving rise to the sub-band gap absorption, but also cause the insulator-to-metal transition in S-hyperdoped Si samples. Based on the experimental results and the calculations by density functional theory, the chemical state of the S species and the formation of the S-dopant states in the band gap of Si are critical in determining the sub-band gap absorptance of hyperdoped Si samples.[[notice]]補正完畢[[journaltype]]國外[[incitationindex]]SCI[[ispeerreviewed]]Y[[booktype]]電子版[[countrycodes]]GB

FACT, the Bur Kinase Pathway, and the Histone Co-Repressor HirC Have Overlapping Nucleosome-Related Roles in Yeast Transcription Elongation

Gene transcription is constrained by the nucleosomal nature of chromosomal DNA. This nucleosomal barrier is modulated by FACT, a conserved histone-binding heterodimer. FACT mediates transcription-linked nucleosome disassembly and also nucleosome reassembly in the wake of the RNA polymerase II transcription complex, and in this way maintains the repression of ‘cryptic’ promoters found within some genes. Here we focus on a novel mutant version of the yeast FACT subunit Spt16 that supplies essential Spt16 activities but impairs transcription-linked nucleosome reassembly in dominant fashion. This Spt16 mutant protein also has genetic effects that are recessive, which we used to show that certain Spt16 activities collaborate with histone acetylation and the activities of a Bur-kinase/Spt4–Spt5/Paf1C pathway that facilitate transcription elongation. These collaborating activities were opposed by the actions of Rpd3S, a histone deacetylase that restores a repressive chromatin environment in a transcription-linked manner. Spt16 activity paralleling that of HirC, a co-repressor of histone gene expression, was also found to be opposed by Rpd3S. Our findings suggest that Spt16, the Bur/Spt4–Spt5/Paf1C pathway, and normal histone abundance and/or stoichiometry, in mutually cooperative fashion, facilitate nucleosome disassembly during transcription elongation. The recessive nature of these effects of the mutant Spt16 protein on transcription-linked nucleosome disassembly, contrasted to its dominant negative effect on transcription-linked nucleosome reassembly, indicate that mutant FACT harbouring the mutant Spt16 protein competes poorly with normal FACT at the stage of transcription-linked nucleosome disassembly, but effectively with normal FACT for transcription-linked nucleosome reassembly. This functional difference is consistent with the idea that FACT association with the transcription elongation complex depends on nucleosome disassembly, and that the same FACT molecule that associates with an elongation complex through nucleosome disassembly is retained for reassembly of the same nucleosome

The structure and function of Alzheimer's gamma secretase enzyme complex

The production and accumulation of the beta amyloid protein (Aβ) is a key event in the cascade of oxidative and inflammatory processes that characterizes Alzheimer’s disease (AD). A multi-subunit enzyme complex, referred to as gamma (γ) secretase, plays a pivotal role in the generation of Aβ from its parent molecule, the amyloid precursor protein (APP). Four core components (presenilin, nicastrin, aph-1, and pen-2) interact in a high-molecular-weight complex to perform intramembrane proteolysis on a number of membrane-bound proteins, including APP and Notch. Inhibitors and modulators of this enzyme have been assessed for their therapeutic benefit in AD. However, although these agents reduce Aβ levels, the majority have been shown to have severe side effects in pre-clinical animal studies, most likely due to the enzymes role in processing other proteins involved in normal cellular function. Current research is directed at understanding this enzyme and, in particular, at elucidating the roles that each of the core proteins plays in its function. In addition, a number of interacting proteins that are not components of γ-secretase also appear to play important roles in modulating enzyme activity. This review will discuss the structural and functional complexity of the γ-secretase enzyme and the effects of inhibiting its activity

Rare and low-frequency coding variants alter human adult height

Height is a highly heritable, classic polygenic trait with ~700 common associated variants identified so far through genome - wide association studies . Here , we report 83 height - associated coding variants with lower minor allele frequenc ies ( range of 0.1 - 4.8% ) and effects of up to 2 16 cm /allele ( e.g. in IHH , STC2 , AR and CRISPLD2 ) , >10 times the average effect of common variants . In functional follow - up studies, rare height - increasing alleles of STC2 (+1 - 2 cm/allele) compromise d proteolytic inhibition of PAPP - A and increased cleavage of IGFBP - 4 in vitro , resulting in higher bioavailability of insulin - like growth factors . The se 83 height - associated variants overlap genes mutated in monogenic growth disorders and highlight new biological candidates ( e.g. ADAMTS3, IL11RA, NOX4 ) and pathways ( e.g . proteoglycan/ glycosaminoglycan synthesis ) involved in growth . Our results demonstrate that sufficiently large sample sizes can uncover rare and low - frequency variants of moderate to large effect associated with polygenic human phenotypes , and that these variants implicate relevant genes and pathways

Genetic fine mapping and genomic annotation defines causal mechanisms at type 2 diabetes susceptibility loci.

We performed fine mapping of 39 established type 2 diabetes (T2D) loci in 27,206 cases and 57,574 controls of European ancestry. We identified 49 distinct association signals at these loci, including five mapping in or near KCNQ1. 'Credible sets' of the variants most likely to drive each distinct signal mapped predominantly to noncoding sequence, implying that association with T2D is mediated through gene regulation. Credible set variants were enriched for overlap with FOXA2 chromatin immunoprecipitation binding sites in human islet and liver cells, including at MTNR1B, where fine mapping implicated rs10830963 as driving T2D association. We confirmed that the T2D risk allele for this SNP increases FOXA2-bound enhancer activity in islet- and liver-derived cells. We observed allele-specific differences in NEUROD1 binding in islet-derived cells, consistent with evidence that the T2D risk allele increases islet MTNR1B expression. Our study demonstrates how integration of genetic and genomic information can define molecular mechanisms through which variants underlying association signals exert their effects on disease