Ruminal Degradation of Rubisco by Beef Cattle Grazing Switchgrass and Big Bluestem

This two-year study was conducted on monocultures of switchgrass and big bluestem to: (1) determine the concentration of the protein ribulose-1,5- bisphosphate carboxylase (Rubisco), found only in the bundle sheath cells of warm-season grasses, in omasal, masticate and fecal samples of grazing cattle; and (2) estimate rumen-escape Rubisco via bundle sheath cells. A quantifying enzyme-linked immunosorbent assay, along with estimates of rumen and lower tract digestibilities, indicated as much as 11% of Rubisco in big bluestem and 13% in switchgrass escaped rumen degradation and was absorbed in the lower tract. Realizing these amounts of escape Rubisco represent a significant level of soluble protein, bundle sheath cells may provide a mechanism allowing soluble protein to escape ruminal degradation

Taking the LEAP (Learner Engaged Advising Programs): VCCS Advising Practices and Recommendations

The Virginia Community College System (VCCS) administration identified the need for adequate and proactive advising programs to foster student success. This paper presents a review of “best practices” in advising to determine commonalities, provides a comparison with current VCCS advising practices, and offers recommendations that support the goal of ensuring high quality advising programs

Complete Genome Sequences of 13 Bacillus subtilis Soil Isolates for Studying Secondary Metabolite Diversity

Bacillus subtilis is a plant-benefiting soil-dwelling Gram-positive bacterium with secondary metabolite production potential. Here, we report the complete genome sequences of 13 B. subtilis strains isolated from different soil samples in Germany and Denmark

The Grizzly, October 12, 2006

Editorial: Call to Responsibility • Letter of Apology • New Shopping Center Near Campus • Spring Registration Just Around the Corner • Preventing Sexual Assault • Sexual Assault Awareness on Campus • New Incentive Program Underway for CAB • Ursinus Students Evoke Shakespeare\u27s Spirit in Two Gentlemen of Verona • Degas: His Work, His Vision, His Camera • Opinions: On Habeas Corpus; Hurt Feelings; Fundamental Importance of Negative Campaigning • Bears Pull Out Victory in Second Half Comeback • Men\u27s Soccer Capture First Win • A Look Into Ursinus College Fall Athleticshttps://digitalcommons.ursinus.edu/grizzlynews/1721/thumbnail.jp

Increased water-use efficiency and reduced CO2 uptake by plants during droughts at a continental-scale

Severe droughts in the Northern Hemisphere cause a widespread decline of agricultural yield, the reduction of forest carbon uptake, and increased CO2 growth rates in the atmosphere. Plants respond to droughts by partially closing their stomata to limit their evaporative water loss, at the expense of carbon uptake by photosynthesis. This trade-off maximizes their water-use efficiency (WUE), as measured for many individual plants under laboratory conditions and field experiments. Here we analyse the 13C/12C stable isotope ratio in atmospheric CO2 to provide new observational evidence of the impact of droughts on the WUE across areas of millions of square kilometres and spanning one decade of recent climate variability. We find strong and spatially coherent increases in WUE along with widespread reductions of net carbon uptake over the Northern Hemisphere during severe droughts that affected Europe, Russia and the United States in 2001–2011. The impact of those droughts on WUE and carbon uptake by vegetation is substantially larger than simulated by the land-surface schemes of six state-of-the-art climate models. This suggests that drought-induced carbon–climate feedbacks may be too small in these models and improvements to their vegetation dynamics using stable isotope observations can help to improve their drought response

A bacterial inflammation sensor regulates c-di-GMP signaling, adhesion, and biofilm formation

Bacteria that colonize animals must overcome, or coexist, with the reactive oxygen species products of inflammation, a front-line defense of innate immunity. Among these is the neutrophilic oxidant bleach, hypochlorous acid (HOCl), a potent antimicrobial that plays a primary role in killing bacteria through nonspecific oxidation of proteins, lipids, and DNA. Here, we report that in response to increasing HOCl levels, Escherichia coli regulates biofilm production via activation of the diguanylate cyclase DgcZ. We identify the mechanism of DgcZ sensing of HOCl to be direct oxidation of its regulatory chemoreceptor zinc-binding (CZB) domain. Dissection of CZB signal transduction reveals that oxidation of the conserved zinc-binding cysteine controls CZB Zn2+ occupancy, which in turn regulates the catalysis of c-di-GMP by the associated GGDEF domain. We find DgcZ-dependent biofilm formation and HOCl sensing to be regulated in vivo by the conserved zinc-coordinating cysteine. Additionally, point mutants that mimic oxidized CZB states increase total biofilm. A survey of bacterial genomes reveals that many pathogenic bacteria that manipulate host inflammation as part of their colonization strategy possess CZB-regulated diguanylate cyclases and chemoreceptors. Our findings suggest that CZB domains are zinc-sensitive regulators that allow host-associated bacteria to perceive host inflammation through reactivity with HOCl

Whitefield News

File includes: January 2016 Volume 3, Issue 7 February 2016 Volume 3, Issue 8 March 2016 Volume 3, Issue 9 April 2016 Volume 3, Issue 10 May 2016 Volume 3, Issue 11 June 2016 Volume 3, Issue 12 July 2016 Volume 4, Issue 1 August 2016 Volume 4, Issue 2 September 2016, Volume 4, Issue 3 October 2016, Volume 4, Issue 4 November 2016, Volume 4, Issue 5 December 2016, Volume 4, Issue