53 research outputs found
Rietveld refinement of the mixed boracite Fe1.59Zn1.41B7O13Br
The structural characterization of the new iron–zinc heptaborate bromide with composition Fe1.59Zn1.41B7O13Br, prepared by chemical transport is reported. A rigid-body model with constrained generalized coordinates was defined in order to hold the positions of the B atoms at reasonable interatomic distances that typically would reach unacceptable values because of the weak scattering power of boron. There are three independent sites for the B atoms of which two are tetrahedrally coordinated. The bond-valence sum around the third B atom, located on a threefold rotation axis, was calculated considering two cases of coordination of boron with oxygens: trigonal-planar and tetrahedral. The contribution of the fourth O atom to the bond-valence sum was found to be only 0.06 v.u., indicating the presence of a very weak bond in the right position to have a distorted tetrahedral coordination in favour of the trigonal-planar coordination for the third B atom. X-ray fluorescence (XRF) was used to determinate the Fe/Zn ratio
Evaluation of host and viral factors associated with severe dengue based on the 2009 WHO classification
Columbus’ footprint in Hispaniola:A paleoenvironmental record of indigenous and colonial impacts on the landscape of the central Cibao Valley, northern Dominican Republic
The 1100-year sedimentary record of Laguna Biajaca reveals human-driven landscape changes in the central Cibao Valley, Dominican Republic, Hispaniola. This sediment-filled cutoff meander is located in close proximity to pre-Colonial archaeological sites and a Colonial urban hub. It provided a nutrient-rich floodable locus for agricultural activities for indigenous communities and for the first introduction of Old World crops and cattle in the Americas. Integration of paleoecological proxies revealed the formation of a clear-water body surrounded by a palm-rich forested landscape around 1100 cal yr BP. Changes in the drainage system were linked to human-driven deforestation, which also changed the composition of the vegetation and fungal communities around the site between AD 1150 and 1500 (800 and 700 cal yr BP). Pre-Colonial modifications of the landscape were primarily the result of fire-use and small-scale clearings. Crop cultivation developed between AD 1250 and 1450 (700–500 cal yr BP). Within decades after Columbus’ arrival in Hispaniola in AD 1492, the first impacts of European colonization included the abandonment of indigenous sites and the introduction of Old World domesticated animals. During the 15th and 16th centuries the area underwent intensive land-clearing that allowed for larger scale crop cultivation. An increase of aquatic vegetation points to sediment-filling around AD 1700 (250 cal yr BP). At that time, cattle breeding expanded and rapidly provoked eutrophication while, concurrently, monocultures became regionally established. This paper provides a framework of past environmental dynamics and offers an opportunity to place archaeological findings in a context of natural and anthropogenic change
HSF-1, HIF-1and HSP90 expression on recombinant Pichia pastoris under fed-batch fermentation
Pichia pastoris is a methylotrophic yeast used as an efficient expression system for heterologous protein production as compared to other expression systems. Considering that every cell must respond to environmental changes to survive and differentiate, determination of endogenous protein related to heat stress responses and hypoxia, it would necessary to establish the temperature and methanol concentration conditions for optimal growth. The aim of this study is characterize the culture conditions through the putative biomarkers in different conditions of temperature and methanol concentration. Three yeast cultures were performed: 3X = 3% methanol -10 °C, 4X = 3% methanol -30 °C, and 5X = 1% methanol -10 °C. The expression level of HIF-1α, HSF-1, HSP-70 and HSP-90 biomarkers were measured by Western blot and in situ detection was performed by immunocytochemistry. The western blot results of HIF-1α and HSP-90 did not indicate statistically significant in the culture conditions studied. Respect to biomarkers location, HIF-1α and HSP-90 presented differences between cultures. In conclusion, the results suggest the cultures in a hypoxic condition produce a high density and yeast cells smaller. Beside the high density would not necessary related with a high production of recombinant proteins in modified-genetically P. pastoris
Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris
HSF-1, HIF-1and HSP90 expression on recombinant Pichia pastoris under fed-batch fermentation
Corrigendum to "European Society for Vascular Surgery (ESVS) 2022 Clinical Practice Guidelines on the Management of Chronic Venous Disease of the Lower Limbs. [Eur J Vasc Endovasc Surg (2022) 63, 184-267]"
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Editor's Choice – European Society for Vascular Surgery (ESVS) 2022 Clinical Practice Guidelines on the Management of Chronic Venous Disease of the Lower Limbs
Corrigendum: Volume 64, Issues 2–3, 2022, 284-285Peer reviewe
The Eighteenth Data Release of the Sloan Digital Sky Surveys: Targeting and First Spectra from SDSS-V
The eighteenth data release of the Sloan Digital Sky Surveys (SDSS) is the
first one for SDSS-V, the fifth generation of the survey. SDSS-V comprises
three primary scientific programs, or "Mappers": Milky Way Mapper (MWM), Black
Hole Mapper (BHM), and Local Volume Mapper (LVM). This data release contains
extensive targeting information for the two multi-object spectroscopy programs
(MWM and BHM), including input catalogs and selection functions for their
numerous scientific objectives. We describe the production of the targeting
databases and their calibration- and scientifically-focused components. DR18
also includes ~25,000 new SDSS spectra and supplemental information for X-ray
sources identified by eROSITA in its eFEDS field. We present updates to some of
the SDSS software pipelines and preview changes anticipated for DR19. We also
describe three value-added catalogs (VACs) based on SDSS-IV data that have been
published since DR17, and one VAC based on the SDSS-V data in the eFEDS field.Comment: Accepted to ApJ
The eighteenth data release of the Sloan Digital Sky Surveys : targeting and first spectra from SDSS-V
The eighteenth data release of the Sloan Digital Sky Surveys (SDSS) is the first one for SDSS-V, the fifth generation of the survey. SDSS-V comprises three primary scientific programs, or "Mappers": Milky Way Mapper (MWM), Black Hole Mapper (BHM), and Local Volume Mapper (LVM). This data release contains extensive targeting information for the two multi-object spectroscopy programs (MWM and BHM), including input catalogs and selection functions for their numerous scientific objectives. We describe the production of the targeting databases and their calibration- and scientifically-focused components. DR18 also includes ~25,000 new SDSS spectra and supplemental information for X-ray sources identified by eROSITA in its eFEDS field. We present updates to some of the SDSS software pipelines and preview changes anticipated for DR19. We also describe three value-added catalogs (VACs) based on SDSS-IV data that have been published since DR17, and one VAC based on the SDSS-V data in the eFEDS field.Publisher PDFPeer reviewe
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