Characterization of the antimicrobial substances produced by Nibribacter radioresistens

This study characterized the antimicrobial substances produced by the radiation-resistant bacterium Nibribacter radioresistens. The antimicrobial substances showed activity against Salmonella Gallinarum, pathogenic Escherichia coli, Bacillus cereus, Streptococcus iniae, and Saccharomyces cerevisiae. The substances showed higher activity against Gram-positive bacteria than against Gram-negative bacteria and yeast. N. radioresistens showed the best growth rate in LB liquid medium at 37ºC; however, production of the antimicrobial substances was not associated with growth. Since the activity of the antimicrobial substances was affected by proteinase K and EDTA, the substances were presumed to be antimicrobial peptides (AMPs). The antimicrobial substances produced by N. radioresistens were unstable at higher temperatures and in acidic and basic pH ranges, and most of the activity was attributed to either low (30 kDa) molecules. When S. Gallinarum was treated with the antimicrobial substances, the cell destruction was acted on the cell envelope. Therefore, we concluded that N. radioresistens produces broad-spectrum and very unstable antimicrobial substances that mostly consist of low- and high-molecular weight peptides

Efficient Ferble Plant Regeneration from Pmtoplasts of Javanica Rice and Their Ploidy Determination by Flow Cytometry

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Cloning of the 52-kDa chitinase gene from Serratia marcescens KCTC2172 and its proteolytic cleavage into an active 35-kDa enzyme

A chitinase gene (pCHI52) encoding the 52-kDa chitinase was isolated from a Serratia marcescens KCTC2172 cosmid library. This chitinase gene consists of 2526 bp with an open reading frame that encodes 485 amino acids. Escherichia coli harboring the pCHI52 gene secreted not only a 52-kDa but also a 35-kDa chitinase into the culture supernatant. We purified both 52-kDa and 35-kDa chitinases using a chitin affinity column and Sephacryl-S-300 gel filtration chromatography. We determined that the 17 N-terminal amino acid sequences of the 52-kDa and the 35-kDa chitinase are identical. Furthermore, a protease obtained from S. marcescens KCTC2172 cleaved the 52-kDa chitinase into the 35-kDa protein with chitinase activity. These results suggest that the 35-kDa chitinase derives from the 52-kDa chitinase by post-translational proteolytic modification. The optimal reaction temperature of 45 degrees C and the optimal pH of 5.5 were identical for both enzymes. The specific activities of the 52-kDa and 35-kDa chitinases on natural swollen chitin were 67 mu mol min(-1) mg(-1) and 60 mu mol min(-1) mg(-1), respectively. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.close414

Anti-Salmonella Activity Modulation of Mastoparan V1—A Wasp Venom Toxin—Using Protease Inhibitors, and Its Efficient Production via an Escherichia coli Secretion System

A previous study highlighted that mastoparan V1 (MP-V1), a mastoparan from the venom of the social wasp Vespula vulgaris, is a potent antimicrobial peptide against Salmonella infection, which causes enteric diseases. However, there exist some limits for its practical application due to the loss of its activity in an increased bacterial density and the difficulty of its efficient production. In this study, we first modulated successfully the antimicrobial activity of synthetic MP-V1 against an increased Salmonella population using protease inhibitors, and developed an Escherichia coli secretion system efficiently producing active MP-V1. The protease inhibitors used, except pepstatin A, significantly increased the antimicrobial activity of the synthetic MP-V1 at minimum inhibitory concentrations (determined against 106 cfu/mL of population) against an increased population (108 cfu/mL) of three different Salmonella serotypes, Gallinarum, Typhimurium and Enteritidis. Meanwhile, the E. coli strain harboring OmpA SS::MP-V1 was identified to successfully secrete active MP-V1 into cell-free supernatant, whose antimicrobial activity disappeared in the increased population (108 cfu/mL) of Salmonella Typhimurium recovered by adding a protease inhibitor cocktail. Therefore, it has been concluded that our challenge using the E. coli secretion system with the protease inhibitors is an attractive strategy for practical application of peptide toxins, such as MP-V1

Multiplicity dependence of light (anti-)nuclei production in p–Pb collisions at sNN=5.02 TeV

The measurement of the deuteron and anti-deuteron production in the rapidity range −1 < y < 0 as a function of transverse momentum and event multiplicity in p–Pb collisions at √sNN = 5.02 TeV is presented. (Anti-)deuterons are identified via their specific energy loss dE/dx and via their time-of- flight. Their production in p–Pb collisions is compared to pp and Pb–Pb collisions and is discussed within the context of thermal and coalescence models. The ratio of integrated yields of deuterons to protons (d/p) shows a significant increase as a function of the charged-particle multiplicity of the event starting from values similar to those observed in pp collisions at low multiplicities and approaching those observed in Pb–Pb collisions at high multiplicities. The mean transverse particle momenta are extracted from the deuteron spectra and the values are similar to those obtained for p and particles. Thus, deuteron spectra do not follow mass ordering. This behaviour is in contrast to the trend observed for non-composite particles in p–Pb collisions. In addition, the production of the rare 3He and 3He nuclei has been studied. The spectrum corresponding to all non-single diffractive p-Pb collisions is obtained in the rapidity window −1 < y < 0 and the pT-integrated yield dN/dy is extracted. It is found that the yields of protons, deuterons, and 3He, normalised by the spin degeneracy factor, follow an exponential decrease with mass number

Production of Λ\Lambda and KS0{\rm K}^{0}_{\rm S} in jets in p-Pb collisions at sNN=5\sqrt{s_{\rm NN}} = 5 TeV and pp collisions at s=7\sqrt{s} = 7 TeV

The production of $\Lambda$ baryons and ${\rm K}^{0}_{\rm S}$ mesons (${\rm V}^{0}$ particles) was measured in p-Pb collisions at $\sqrt{s_{\rm NN}} = 5$ TeV and pp collisions at $\sqrt{s} = 7$ TeV with ALICE at the LHC. The production of these strange particles is studied separately for particles associated with hard scatterings and the underlying event to shed light on the baryon-to-meson ratio enhancement observed at intermediate transverse momentum ($p_{\rm T}$) in high multiplicity pp and p-Pb collisions. Hard scatterings are selected on an event-by-event basis with jets reconstructed with the anti-$k_{\rm T}$ algorithm using charged particles. The production of strange particles associated with jets $p_{\rm T,\;jet}^{\rm ch}>10$ GeV/$c$ is reported as a function of $p_{\rm T}$ in both systems; and its dependence on $p_{\rm T}$ with jets $p_{\rm T,\;jet}^{\rm ch}>20$ GeV/$c$ and on angular distance from the jet axis, $R({\rm V}^{0},\;{\rm jet})$, for jets with $p_{\rm T,\;jet}^{\rm ch} > 10$ GeV/$c$ are reported in p-Pb collisions. The results are compared with the strange particle production in the underlying event. The $\Lambda/{\rm K}^{0}_{\rm S}$ ratio associated with jets in p-Pb collisions for $R({\rm V}^{0},\;{\rm jet})<0.4$ is consistent with the ratio measured in pp collisions and with the expectation of jets fragmenting in vacuum given by the PYTHIA event generator

Λc+\Lambda^+_c production in pppp and in pp-Pb collisions at sNN\sqrt {s_{NN}}=5.02 TeV

International audienceThe production cross section of prompt $\mathrm{\Lambda_{c}^{+}}$ charm baryons was measured with the ALICE detector at the LHC at midrapidity in proton-proton (pp) and proton-lead (p-Pb) collisions at a centre-of-mass energy per nucleon pair of $\sqrt{s_\mathrm{NN}} = 5.02$ TeV. The $\mathrm{\Lambda_{c}^{+}}$ and $\rm {\overline{\Lambda}{}_c^-}$ baryons were reconstructed in the hadronic decay channels $\rm \Lambda_{c}^{+} \rightarrow p K^{-}\pi^{+}$ and $\rm \Lambda_{c}^{+}\to p K^{0}_{S}$ and respective charge conjugates. The measured differential cross sections as a function of transverse momentum ($p_{\rm T}$) and the $p_{\rm T}$-integrated $\mathrm{\Lambda_{c}^{+}}$ production cross section in pp and in p-Pb collisions are presented. The $\mathrm{\Lambda_{c}^{+}}$ nuclear modification factor ($R_\mathrm{pPb}$), calculated from the cross sections in pp and in p-Pb collisions, is presented and compared with the $R_\mathrm{pPb}$ of D mesons. The $\mathrm {\Lambda_{c}^{+}}/\mathrm {D^0}$ ratio is also presented and compared with the light-flavour baryon-to-meson ratios p$/\pi$ and $\Lambda /\mathrm {K^0_S}$, and measurements from other LHC experiments. The results are compared to predictions from model calculations and Monte Carlo event generators

Measurement of prompt D0^{0}, D+^{+}, D∗+^{*+}, and DS+ {\mathrm{D}}_{\mathrm{S}}^{+} production in p–Pb collisions at sNN \sqrt{{\mathrm{s}}_{\mathrm{NN}}} = 5.02 TeV

International audienceThe measurement of the production of prompt D$^{0}$, D$^{+}$, D$^{*+}$, and ${\mathrm{D}}_{\mathrm{S}}^{+}$ mesons in proton–lead (p–Pb) collisions at the centre-of-mass energy per nucleon pair of $\sqrt{s_{\mathrm{NN}}}$ = 5.02 TeV, with an integrated luminosity of 292 ± 11 μb$^{−1}$, are reported. Differential production cross sections are measured at mid-rapidity (−0.96 < y$_{cms}$< 0.04) as a function of transverse momentum (p$_{T}$) in the intervals 0 < p$_{T}$< 36 GeV/c for D$^{0}$, 1 < p$_{T}$< 36 GeV/c for D$^{+}$ and D$^{*+}$, and 2 < p$_{T}$< 24 GeV/c for D$^{+}$ mesons. For each species, the nuclear modification factor R$_{pPb}$ is calculated as a function of p$_{T}$ using a proton-proton (pp) ref- erence measured at the same collision energy. The results are compatible with unity in the whole p$_{T}$ range. The average of the non-strange D mesons R$_{pPb}$ is compared with theoretical model predictions that include initial-state effects and parton transport model predictions. The p$_{T}$ dependence of the D$^{0}$, D$^{+}$, and D$^{*+}$ nuclear modification factors is also reported in the interval 1 < p$_{T}$< 36 GeV/c as a function of the collision centrality, and the central-to-peripheral ratios are computed from the D-meson yields measured in different centrality classes. The results are further compared with charged-particle measurements and a similar trend is observed in all the centrality classes. The ratios of the p$_{T}$-differential cross sections of D$^{0}$, D$^{+}$, D$^{*+}$, and ${\mathrm{D}}_{\mathrm{S}}^{+}$ mesons are also reported. The ${\mathrm{D}}_{\mathrm{S}}^{+}$ and D$^{+}$ yields are compared as a function of the charged-particle multiplicity for several p$_{T}$ intervals. No modification in the relative abundances of the four species is observed with respect to pp collisions within the statistical and systematic uncertainties