Optimization of cultural conditions for submerged state fermentation of di-gested biogas slurry for production of lignocellulolytic enzymes using Phanaerochaete chrysosporium MTCC 787

Growing environmental concerns and increasing demands from end-use sectors have increased the glob-al market for microbial products. Optimizations of production parameters hold great importance for the industry. The present study was aimed at optimization of submerged state fermentation conditions for production of lignocelluloly-tic enzymes from digested biogas slurry by Phanaerochaete chrysosporium MTCC 787. Enzyme activities for differ-ent enzymes i.e. endoglucanase, exoglucanase, ?-glucosidase; xylanase and mannanase; laccase, lignin peroxidase and manganese peroxidise, using P. chrysosporium MTCC 787 were maximum at 50% concentration of digested slur-ry and showed maximum value of xylanase i.e. 187.41U/ml. Effect of temperature (25°C, 30°C and 35°C) on lignocellu-losic bioconversion showed that at 30°C, maximum value of manganese peroxidise (167.5 U/ml) was obtained. High-est enzyme activites were obtained at selected inoculum size i.e. 10?spores/ml, e.g. 85.29 U/ml xylanase was ob-tained. Incubation period of 8 days and pH of 7.0 came out to be best conditions for P. chrysosporium MTCC787 to produce maximum enzyme activity e.g. xylanase 95.47U/ml at pH 7.0 and xylanase 144.96U/ml at 8 day incu-bation.This work presents a novel concept in optimization of fermentation process to produce lignocellulolytic en-zymes as this work is focussed on utilization of digested biogas slurry as a substrate for enzyme production and enhancement of the production with microbial source, which is environment friendly

Analysis of the growth profile, biochemical composition and nutrient removal efficacy of Spirulina sp. NCIM 5143

In the present manuscript, the growth profile of the microalgae Spirulina sp. NCIM 5143 was studied. Screening was performed on four commercial media, i.e., blue‒green-11 (BG-11), Bold’s basal medium (BBM), algal culture medium (ACM), Zarrouk’s medium (ZM), and different concentrations (20%, 40%, 60%, 80%, and 100%) of unsterilized dairy effluent (UDE). Characterization of biomass was performed to assess its biochemical composition through various assays. Elemental composition and bioactive compound analysis were accomplished by inductively coupled plasma-atomic emission spectroscopy (ICP‒AES) and gas chromatography‒mass spectrometry (GC‒MS), respectively. The results revealed that maximum values of most of the parameters, i.e., optical density (0.21), chlorophyll (2.00 mg/l), proteins (119.17 mg/l), and wet (4.06 g/l) and dry biomass weight (0.28 g/l), were found on ZM. For UDE, maximum growth parameters and the highest nutrient removal efficiency were obtained at 100% concentration. Biochemical analysis revealed that total Kjeldahl nitrogen (7.14±0.49%), crude protein (48.23± 3.34%), total antioxidant activity (3.07±0.03 mg AAE/g), and total phenols (8.88±1.93 mg GAE/g) were present in the biomass. Elemental and GC‒MS analysis detected essential micronutrients and many bioactive compounds, respectively. Hence, this study proved that Spirulina sp. NCIM 5143 has the potential for the management of waste dairy effluent. This study also showed its cost-effectiveness, as the dairy effluent analyzed is used without any kind of sterilization. In addition, its biomass is rich in several essential elements, antioxidants, and bioactive compounds of therapeutic and nutraceutical importance

Effect of Fusarium sp. On Paddy Straw Digestibility and Biogas Production

Chopped and moist paddy straw was pretreated with Fusarium sp. to enhance its digestibility and biogas production. The potential of microbial pretreatment of paddy straw was investigated at regular interval of 5, 10, 15 and 20 days by determining the change in Chemical composition of paddy straw like cellulose, hemicellulose, lignin and silica contents. The pretreated straw was used for biogas production in 2l capacity biogas digesters. Results indicate that the cellulose, hemicellulose, lignin and silica contents decreased by 17.2%, 3.4%, 27.1% & 16.5% respectively. Biogas production also increased by 53.8% in 10 days pretreated samples. The significantly higher reduction of silica along with lignin content in the pretreated straw indicates that removal of silica by Fusarium sp. might be more responsible for increasing paddy straw digestibility and biogas production

Comparative evaluation of different pretreatment methods on biogas production from paddy straw

The present investigations observed the effect of chemical, enzymatic, biological and micro-wave pretreatment on paddy straw for enhancement of biogas production. Chopped and soaked paddy straw was subjected to chemicals Na2CO3 (1%) and NaOH (2%) concentrations, microwave irradiation (720 watt, 30 min), fungal (spawn impregnated, 7 days) and crude silicase (24 hrs) pretreatment. The proximate and chemical analysis showed 16.0% and 12.1% reduction in lignin and silica content in the case of Na2CO3 pretreated paddy straw whereas 23.0% and 46.8% reduction was observed in enzymatic pretreatment with 43.7% and 31.1% enhancement in biogas production respectively. This clearly indi-cates that Pleurotus ostreatus MTCC 142 is silicolytic as well as ligninolytic in nature. Enzymatic pre-treatment was also compared with, microwave (30 min) and fungal pretreatment which showed 31.2% and 32.8% reduction in silica content enhancing biogas production by 19.7% and 42.6% respectively. NaOH pretreatment showed a maximum increase in biogas production i.e. 49.7% as compared to 1% pretreated sample which showed 28.5% enhancement. The results indicated that the NaOH pretreatment was one of the potential methods to increase biogas production of paddy straw

Optimization of biogas production from water hyacinth (Eichhornia crassipes)

The present investigation reports the optimization of process parameters for biogas production from water hyacinth (Eichhornia crassipes). The different parameters like particle size, inoculum concentration, incubation temperature, metal ions and pH were optimized for biogas production. Maximum biogas was observed with water hyacinth of 1cm size, 40 % inoculum concentration. The temperature of 45°C along with neutral pH i.e. 7 was found to be most suitable for biogas production in the presence of manganese chloride (0.2 mM). Under optimized conditions, 44.9 l biogas/kg water hyacinth, 360.09 l/kg total solids and 397.95 l biogas/kg volatile solids were produced in a period of 40 days. The water hyacinth has proven to be a good source of biogas production and thus can be utilized as a potential feedstock for the biogas production

Análisis sistémico de las externalidades del mercado de bioetanol

Trabajo de investigaciónEn Colombia a partir de la resolución 40108 de 2018, se aumentó el porcentaje de mezcla del combustibles llegando a un 10% (90% combustibles fósiles 10% biocombustibles), actualmente para suplir esta demanda, las productoras de bioetanol requieren emplear al 100% su capacidad instalada, mejorar sus prácticas de cultivo de caña de azúcar y hacer uso de nuevas hectáreas, por tanto, el presente trabajo de grado tiene como objetivo realizar un análisis sistémico de las externalidades del mercado de bioetanol.INTRODUCCIÓN 1. Formulación del trabajo 2. Marco referencial 3. Diagrama propuesto 4. Conclusiones generales BibliografiaPregradoEconomist

Optimization of cultural conditions for submerged state fermentation of di-gested biogas slurry for production of lignocellulolytic enzymes using Phanaerochaete chrysosporium MTCC 787

Growing environmental concerns and increasing demands from end-use sectors have increased the glob-al market for microbial products. Optimizations of production parameters hold great importance for the industry. The present study was aimed at optimization of submerged state fermentation conditions for production of lignocelluloly-tic enzymes from digested biogas slurry by Phanaerochaete chrysosporium MTCC 787. Enzyme activities for differ-ent enzymes i.e. endoglucanase, exoglucanase, ?-glucosidase; xylanase and mannanase; laccase, lignin peroxidase and manganese peroxidise, using P. chrysosporium MTCC 787 were maximum at 50% concentration of digested slur-ry and showed maximum value of xylanase i.e. 187.41U/ml. Effect of temperature (25°C, 30°C and 35°C) on lignocellu-losic bioconversion showed that at 30°C, maximum value of manganese peroxidise (167.5 U/ml) was obtained. High-est enzyme activites were obtained at selected inoculum size i.e. 10?spores/ml, e.g. 85.29 U/ml xylanase was ob-tained. Incubation period of 8 days and pH of 7.0 came out to be best conditions for P. chrysosporium MTCC787 to produce maximum enzyme activity e.g. xylanase 95.47U/ml at pH 7.0 and xylanase 144.96U/ml at 8 day incu-bation.This work presents a novel concept in optimization of fermentation process to produce lignocellulolytic en-zymes as this work is focussed on utilization of digested biogas slurry as a substrate for enzyme production and enhancement of the production with microbial source, which is environment friendly

Performance Evaluation of Solid State Digester for Biogas Production using Biologically Pretreated Straw International Journal of Agriculture, Environment & Biotechnology

Abstract The present paper reports biogas production from biologically pretreated paddy straw in a solid state digester made of HDPE plastic (124litre capacity). The trial was conducted with a mixture of 10 kg pretreated paddy straw, 20 kg cattle dung slurry, 20 kg cattle dung and 20 litre water. The chemical and proximate analysis of the feed was done and biogas production profile was studied over a period of one month. Parallel trial was also conducted with untreated paddy straw. The result indicated that 253.89 litre biogas/kg pretreated paddy straw was produced, which is 39.3% higher than the untreated paddy straw. Highlights • Fabricated digester worked well for biogas generation from untreatedas well as pretreated paddy straw. • 39.3% enhancement in biogas production was observed in case of pretreated straw

Partial Purification and Characterization of Cellulolytic Enzymes Extracted from Trichoderma reesei Inoculated Digested Biogas Slurry

Alternative sources of energy are the demand of the modern world. For generating different alternate fuels, utilization of lignocellulolytic biomass is on top of the priority list, for which cellulase enzymes are required specifically. In this context, the aim of the presented paper was the partial purification and characterization of cellulolytic enzymes from Trichoderma reesei inoculated digested biogas slurry. The enzyme was produced at previously standardized conditions (Incubation period: 15 day, Spore concentration: 108 spores/ml, Slurry concentration: 25%). The cellulolytic enzymes viz. CMCase, Cellobiase and FilterPaperase produced were then partially purified by ammonium sulphate precipitation (0-30 and 30-80%) and dialysis followed by ion exchange chromatography using DEAE-cellulose column. Twelve fold purification was achieved for cellobiase. Specific activity of 20.18 U/mg was measured. Two isoforms of Cellobaise (C-I and C-II) were found with 21 and 32 fold purification, respectively. Upon characterization, the optimal pH and optimal temperature values for cellobiase came out to be 7.5 and 55°C for C-I and 25°C for C-II. Carboxymethyl cellulase was partially purified up to 10.4 fold with specific activity of 1.87 U/mg of protein and Fpase was purified to 11.3 fold with 1.47 U/mg of protein specific activity. Partially purified enzyme activities were compared with that of commercial enzymes. This is a novel work where cellulases were extracted and partially purified from digested slurry from biogas plant, which is very significant with reference to not only disposal of digested biogas slurry but also its value addition for industrial applications

Comparative evaluation of different pretreatment methods on biogas production from paddy straw

The present investigations observed the effect of chemical, enzymatic, biological and micro-wave pretreatment on paddy straw for enhancement of biogas production. Chopped and soaked paddy straw was subjected to chemicals Na2CO3 (1%) and NaOH (2%) concentrations, microwave irradiation (720 watt, 30 min), fungal (spawn impregnated, 7 days) and crude silicase (24 hrs) pretreatment. The proximate and chemical analysis showed 16.0% and 12.1% reduction in lignin and silica content in the case of Na2CO3 pretreated paddy straw whereas 23.0% and 46.8% reduction was observed in enzymatic pretreatment with 43.7% and 31.1% enhancement in biogas production respectively. This clearly indi-cates that Pleurotus ostreatus MTCC 142 is silicolytic as well as ligninolytic in nature. Enzymatic pre-treatment was also compared with, microwave (30 min) and fungal pretreatment which showed 31.2% and 32.8% reduction in silica content enhancing biogas production by 19.7% and 42.6% respectively. NaOH pretreatment showed a maximum increase in biogas production i.e. 49.7% as compared to 1% pretreated sample which showed 28.5% enhancement. The results indicated that the NaOH pretreatment was one of the potential methods to increase biogas production of paddy straw