Chemical and dynamical processes in the mesospheric emissive layer. First results of stereoscopic observations

[1] The mesospheric emissive layer is an efficient tracer of the dynamical processes propagating in the atmosphere at that level. CCD images in the near infrared taken from the ground at slant angles often reveal the existence of wavy fields. A series of such images has been transformed, using matrix operations, producing a downward satellite-type view that covers a circular area of radius ∼1000 km at the altitude of the layer. The Fourier characteristics of the wave system are measured using a Morlet-type wavelet generator function with horizontal wavelengths of mostly ∼20–40 km and 100–150 km and temporal periods of ∼15–30 min. An oxygen-hydrogen model is used to evaluate the response of the emissive layer to a progressive density wave. The altitude of the layer is modulated with an amplitude of ∼0.8–1.8 km when a density wave propagates vertically. The layer thickness is slightly modulated and is equal to ∼7 km. Stereoscopic pairs of photographs taken simultaneously on 8–9 September 2000 at the Château-Renard and Pic du Midi observatories are used to obtain surface maps of the emission layer barycenter altitude. A stereocorrelation method suitable for low contrast objects without discrete contours is employed. Preliminary results for areas ∼50 × 50 km2 are presented. The surface maps of the layer barycenter altitude depict the existence of waves. They show the same wavy structure and compare favorably with the maps showing the emission intensity

APETx4, a novel sea anemone toxin and a modulator of the cancer-relevant potassium channel K_V10.1

The human ether-à-go-go channel (hEag1 or KV10.1) is a cancer-relevant voltage-gated potassium channel that is overexpressed in a majority of human tumors. Peptides that are able to selectively inhibit this channel can be lead compounds in the search for new anticancer drugs. Here, we report the activity-guided purification and electrophysiological characterization of a novel KV10.1 inhibitor from the sea anemone Anthopleura elegantissima. Purified sea anemone fractions were screened for inhibitory activity on KV10.1 by measuring whole-cell currents as expressed in Xenopus laevis oocytes using the two-microelectrode voltage clamp technique. Fractions that showed activity on Kv10.1 were further purified by RP-HPLC. The amino acid sequence of the peptide was determined by a combination of MALDI- LIFT-TOF/TOF MS/MS and CID-ESI-FT-ICR MS/MS and showed a high similarity with APETx1 and APETx3 and was therefore named APETx4. Subsequently, the peptide was electrophysiologically characterized on KV10.1. The selectivity of the toxin was investigated on an array of voltage-gated ion channels, including the cardiac human ether-à-go-go-related gene potassium channel (hERG or Kv11.1). The toxin inhibits KV10.1 with an IC50 value of 1.1 μM. In the presence of a similar toxin concentration, a shift of the activation curve towards more positive potentials was observed. Similar to the effect of the gating modifier toxin APETx1 on hERG, the inhibition of Kv10.1 by the isolated toxin is reduced at more positive voltages and the peptide seems to keep the channel in a closed state. Although the peptide also induces inhibitory effects on other KV and NaV channels, it exhibits no significant effect on hERG. Moreover, APETx4 induces a concentration-dependent cytotoxic and proapoptotic effect in various cancerous and noncancerous cell lines. This newly identified KV10.1 inhibitor can be used as a tool to further characterize the oncogenic channel KV10.1 or as a scaffold for the design and synthesis of more potent and safer anticancer drugs

Generic 3D Representation via Pose Estimation and Matching

Though a large body of computer vision research has investigated developing generic semantic representations, efforts towards developing a similar representation for 3D has been limited. In this paper, we learn a generic 3D representation through solving a set of foundational proxy 3D tasks: object-centric camera pose estimation and wide baseline feature matching. Our method is based upon the premise that by providing supervision over a set of carefully selected foundational tasks, generalization to novel tasks and abstraction capabilities can be achieved. We empirically show that the internal representation of a multi-task ConvNet trained to solve the above core problems generalizes to novel 3D tasks (e.g., scene layout estimation, object pose estimation, surface normal estimation) without the need for fine-tuning and shows traits of abstraction abilities (e.g., cross-modality pose estimation). In the context of the core supervised tasks, we demonstrate our representation achieves state-of-the-art wide baseline feature matching results without requiring apriori rectification (unlike SIFT and the majority of learned features). We also show 6DOF camera pose estimation given a pair local image patches. The accuracy of both supervised tasks come comparable to humans. Finally, we contribute a large-scale dataset composed of object-centric street view scenes along with point correspondences and camera pose information, and conclude with a discussion on the learned representation and open research questions.Comment: Published in ECCV16. See the project website http://3drepresentation.stanford.edu/ and dataset website https://github.com/amir32002/3D_Street_Vie

Growing blood vessels in space : preparation studies of the SPHEROIDS project using related ground-based studies

Endothelial cells (ECs) grow as single layers on the bottom surface of cell culture flasks under normal (1g) culture conditions. In numerous experiments using simulated microgravity we noticed that the ECs formed three-dimensional, tube-like cell aggregates resembling the intima of small, rudimentary blood vessels. The SPHEROIDS project has now shown that similar processes occur in space. For the first time, we were able to observe scaffold-free growth of human ECs into multicellular spheroids and tubular structures during an experiment in real microgravity. With further investigation of the space samples we hope to understand endothelial 3D growth and to improve the in vitro engineering of biocompatible vessels which could be used in surgery

From Multiview Image Curves to 3D Drawings

Reconstructing 3D scenes from multiple views has made impressive strides in recent years, chiefly by correlating isolated feature points, intensity patterns, or curvilinear structures. In the general setting - without controlled acquisition, abundant texture, curves and surfaces following specific models or limiting scene complexity - most methods produce unorganized point clouds, meshes, or voxel representations, with some exceptions producing unorganized clouds of 3D curve fragments. Ideally, many applications require structured representations of curves, surfaces and their spatial relationships. This paper presents a step in this direction by formulating an approach that combines 2D image curves into a collection of 3D curves, with topological connectivity between them represented as a 3D graph. This results in a 3D drawing, which is complementary to surface representations in the same sense as a 3D scaffold complements a tent taut over it. We evaluate our results against truth on synthetic and real datasets.Comment: Expanded ECCV 2016 version with tweaked figures and including an overview of the supplementary material available at multiview-3d-drawing.sourceforge.ne

Ultrafast supercontinuum spectroscopy of carrier multiplication and biexcitonic effects in excited states of PbS quantum dots

We examine the multiple exciton population dynamics in PbS quantum dots by ultrafast spectrally-resolved supercontinuum transient absorption (SC-TA). We simultaneously probe the first three excitonic transitions over a broad spectral range. Transient spectra show the presence of first order bleach of absorption for the 1S_h-1S_e transition and second order bleach along with photoinduced absorption band for 1P_h-1P_e transition. We also report evidence of the one-photon forbidden 1S_{h,e}-1P_{h,e} transition. We examine signatures of carrier multiplication (multiexcitons for the single absorbed photon) from analysis of the first and second order bleaches, in the limit of low absorbed photon numbers (~ 10^-2), at pump energies from two to four times the semiconductor band gap. The multiexciton generation efficiency is discussed both in terms of a broadband global fit and the ratio between early- to long-time transient absorption signals.. Analysis of population dynamics shows that the bleach peak due to the biexciton population is red-shifted respect the single exciton one, indicating a positive binding energy.Comment: 16 pages, 5 figure

Angular redistribution of near-infrared emission from quantum dots in 3D photonic crystals

We study the angle-resolved spontaneous emission of near-infrared light sources in 3D photonic crystals over a wavelength range from 1200 to 1550 nm. To this end PbSe quantum dots are used as light sources inside titania inverse opal photonic crystals. Strong deviations from the Lambertian emission profile are observed. An attenuation of 60 % is observed in the angle dependent radiant flux emitted from the samples due to photonic stop bands. At angles that correspond to the edges of the stop band the emitted flux is increased by up to 34 %. This increase is explained by the redistribution of Bragg-diffracted light over the available escape angles. The results are quantitatively explained by an expanded escape-function model. This model is based on diffusion theory and adapted to photonic crystals using band structure calculations. Our results are the first angular redistributions and escape functions measured at near-infrared, including telecom, wavelengths. In addition, this is the first time for this model to be applied to describe emission from samples that are optically thick for the excitation light and relatively thin for the photoluminesence light.Comment: 24 pages, 8 figures (current format = single column, double spaced

Synthesis of highly luminescent wurtzite CdSe/CdS giant-shell nanocrystals using a fast continuous injection route

We synthesized CdSe/CdS giant-shell nanocrystals, with a CdSe core diameter between 2.8 nm and 5.5 nm, and a CdS shell thickness of up to 7–8 nm (equivalent to about 20 monolayers of CdS). Both the core and shell have a wurtzite crystal structure, yielding epitaxial growth of the shell and nearly defect-free crystals. As a result, the photoluminescence (PL) quantum efficiency (QE) is as high as 90%. Quantitative PL measurements at various excitation wavelengths allow us to separate the nonradiative decay into contributions from interface and surface trapping, giving us pathways for future optimization of the structure. In addition, the NCs do not blink, and the giant shell and concurring strong electron delocalization efficiently suppress Auger recombination, yielding a biexciton lifetime of about 15 ns. The corresponding biexciton PL QE equals 11% in 5.5/18.1 nm CdSe/CdS. Variable-temperature time-resolved PL and PL under magnetic fields further reveal that the emission at cryogenic temperature originates from a negative trion-state, in agreement with other CdSe/CdS giant-shell systems reported in the literature

Molecular jets driven by high-mass protostars: a detailed study of the IRAS 20126+4104 jet

We present here an extensive analysis of the protostellar jet driven by IRAS 20126+4104, deriving the kinematical, dynamical, and physical conditions of the H2 gas along the flow. The jet has been investigated by means of near-IR H2 and [FeII] narrow-band imaging, high resolution spectroscopy of the 1-0S(1) line (2.12 um), NIR (0.9-2.5 um) low resolution spectroscopy, along with ISO-SWS and LWS spectra (from 2.4 to 200 um). The flow shows a complex morphology. In addition to the large-scale jet precession presented in previous studies, we detect a small-scale wiggling close to the source, that may indicate the presence of a multiple system. The peak radial velocities of the H2 knots range from -42 to -14 km s^-1 in the blue lobe, and from -8 to 47 km s^-1 in the red lobe. The low resolution spectra are rich in H_2 emission, and relatively faint [FeII] (NIR), [OI] and [CII] (FIR) emission is observed in the region close to the source. A warm H2 gas component has an average excitation temperature that ranges between 2000 K and 2500 K. Additionally, the ISO-SWS spectrum reveals the presence of a cold component (520 K), that strongly contributes to the radiative cooling of the flow and plays a major role in the dynamics of the flow. The estimated L(H2) of the jet is 8.2+/-0.7 L_sun, suggesting that IRAS20126+4104 has an accretion rate significantly increased compared to low-mass YSOs. This is also supported by the derived mass flux rate from the H2 lines (Mflux(H2)~7.5x10^-4 M_sun yr^-1). The comparison between the H2 and the outflow parameters strongly indicates that the jet is driving, at least partially, the outflow. As already found for low-mass protostellar jets, the measured H2 outflow luminosity is tightly related to the source bolometric luminosity.Comment: 16 pages, 12 figures. A&A accepte