Interpretation of cone penetration test data in layered soils using cavity expansion analysis

Cavity expansion theory plays an important role in many geotechnical engineering problems, including the cone penetration test (CPT). One of the challenges of interpreting CPT data is the delineation of interfaces between soil layers and the identification of distinct thin layers, a process which relies on an in-depth understanding of the relationship between penetrometer readings and soil properties. In this paper, analytical cavity expansion solutions in two concentric regions of soil are applied to the interpretation of CPT data, with a specific focus on the layered effects during penetration. The solutions provide a large-strain analysis of cavity expansion in two concentric regions for dilatant elastic-perfectly plastic material. The analysis of CPT data in two-layered soils highlights the effect of respective soil properties (strength, stiffness) on CPT measurements within the influence zones around the two-soil interface. Results show good comparisons with numerical results and elastic solutions. A simple superposition method of the two-layered analytical approach is applied to the analysis of penetration in multilayered soils. A good comparison with field data and numerical results is obtained. It is illustrated that the proposed parameters effectively capture the influence of respective soil properties in the thin-layer analysis. It is also shown that results based on this analysis have better agreement with numerical results compared with elastic solutions

Density effect in Cu K-shell ionization by 5.1-GeV electrons

We have made an absolute measurement of the Cu K-shell impact ionization cross section by 5.1-GeV electrons, which demonstrates directly a density effect predicted by Fermi in 1940. By determining the ratio of the K x-ray yield from a thin front and back layer of the target by a novel grazing emission method, we have verified the effect of transition radiation on the x-ray production, suggested by Sorensen and reported by Bak et al

Beam on Nonlinear Winkler Foundation and Modified Neutral Plane Solution for Calculating Downdrag Settlement

The neutral plane solution has been widely used to estimate downdrag settlements and drag loads mobilized in piles in consolidating soil profiles. Pile settlement is typically assumed equal to soil settlement at the neutral plane depth corresponding to effective stress conditions at the end of consolidation. This paper demonstrates that, in general, pile settlement is not equal to soil settlement at the neutral plane depth; rather, it is the relative velocity between the pile and soil that is zero at the neutral plane depth. A beam on nonlinear Winkler foundation (BNWF) solution, in which the shaft friction capacity is proportional to effective stress, is utilized to demonstrate that pile settlement is not equal to soil settlement at the neutral plane depth, because the neutral plane depth evolves as consolidation progresses. The BNWF solution also shows that pile settlement depends on drainage conditions, with more settlement occurring when consolidation occurs first near the top of the consolidating soil layer, and less settlement occurring when consolidation initiates at the bottom. A modified neutral plane solution that is amenable to hand calculation is formulated to account for the evolution of neutral plane depth on pile settlement. Finally, the proposed BNWF and modified neutral plane solutions are compared with measurements of downdrag settlement from a centrifuge test program. The proposed methods produced more accurate estimates of pile settlement than the traditional neutral plane solution. © 2013 American Society of Civil Engineers

Investigating the effects of particle shape on normal compression and overconsolidation using DEM

Discrete element modelling of normal compression has been simulated on a sample of breakable two-ball clumps and compared to that of spheres. In both cases the size effect on strength is assumed to be that of real silica sand. The slopes of the normal compression lines are compared and found to be consistent with the proposed equation of the normal compression line. The values of the coefficient of earth pressure at rest K0,nc are also compared and related to the critical state fiction angles for the two materials. The breakable samples have then been unloaded to establish the stress ratios on unloading. At low overconsolidation ratios the values of K0 follow a well-established empirical relationship and realistic Poisson ratios are observed. On progressive unloading both samples head towards passive failure, and the values of the critical state lines in extension in q–p' space are found to be consistent with the critical state angles deduced from the values of K0 during normal compression. The paper highlights the important role of particle shape in governing the stress ratio during both normal compression and subsequent overconsolidation

Insights into the Binding of Phenyltiocarbamide (PTC) Agonist to Its Target Human TAS2R38 Bitter Receptor

Humans' bitter taste perception is mediated by the hTAS2R subfamily of the G protein-coupled membrane receptors (GPCRs). Structural information on these receptors is currently limited. Here we identify residues involved in the binding of phenylthiocarbamide (PTC) and in receptor activation in one of the most widely studied hTAS2Rs (hTAS2R38) by means of structural bioinformatics and molecular docking. The predictions are validated by site-directed mutagenesis experiments that involve specific residues located in the putative binding site and trans-membrane (TM) helices 6 and 7 putatively involved in receptor activation. Based on our measurements, we suggest that (i) residue N103 participates actively in PTC binding, in line with previous computational studies. (ii) W99, M100 and S259 contribute to define the size and shape of the binding cavity. (iii) W99 and M100, along with F255 and V296, play a key role for receptor activation, providing insights on bitter taste receptor activation not emerging from the previously reported computational models

Characterization of the Modes of Binding between Human Sweet Taste Receptor and Low-Molecular-Weight Sweet Compounds

One of the most distinctive features of human sweet taste perception is its broad tuning to chemically diverse compounds ranging from low-molecular-weight sweeteners to sweet-tasting proteins. Many reports suggest that the human sweet taste receptor (hT1R2–hT1R3), a heteromeric complex composed of T1R2 and T1R3 subunits belonging to the class C G protein–coupled receptor family, has multiple binding sites for these sweeteners. However, it remains unclear how the same receptor recognizes such diverse structures. Here we aim to characterize the modes of binding between hT1R2–hT1R3 and low-molecular-weight sweet compounds by functional analysis of a series of site-directed mutants and by molecular modeling–based docking simulation at the binding pocket formed on the large extracellular amino-terminal domain (ATD) of hT1R2. We successfully determined the amino acid residues responsible for binding to sweeteners in the cleft of hT1R2 ATD. Our results suggest that individual ligands have sets of specific residues for binding in correspondence with the chemical structures and other residues responsible for interacting with multiple ligands