Primitive layered gabbros from fast-spreading lower oceanic crust

Three-quarters of the oceanic crust formed at fast-spreading ridges is composed of plutonic rocks whose mineral assemblages, textures and compositions record the history of melt transport and crystallization between the mantle and the sea floor. Despite the importance of these rocks, sampling them in situ is extremely challenging owing to the overlying dykes and lavas. This means that models for understanding the formation of the lower crust are based largely on geophysical studies and ancient analogues (ophiolites) that did not form at typical mid-ocean ridges. Here we describe cored intervals of primitive, modally layered gabbroic rocks from the lower plutonic crust formed at a fast-spreading ridge, sampled by the Integrated Ocean Drilling Program at the Hess Deep rift. Centimetre-scale, modally layered rocks, some of which have a strong layering-parallel foliation, confirm a long-held belief that such rocks are a key constituent of the lower oceanic crust formed at fast-spreading ridges. Geochemical analysis of these primitive lower plutonic rocks-in combination with previous geochemical data for shallow-level plutonic rocks, sheeted dykes and lavas-provides the most completely constrained estimate of the bulk composition of fast-spreading oceanic crust so far. Simple crystallization models using this bulk crustal composition as the parental melt accurately predict the bulk composition of both the lavas and the plutonic rocks. However, the recovered plutonic rocks show early crystallization of orthopyroxene, which is not predicted by current models of melt extraction from the mantle and mid-ocean-ridge basalt differentiation. The simplest explanation of this observation is that compositionally diverse melts are extracted from the mantle and partly crystallize before mixing to produce the more homogeneous magmas that erupt

Magmatism, serpentinization and life: Insights through drilling the Atlantis Massif (IODP Expedition 357)

IODP Expedition 357 used two seabed drills to core 17 shallow holes at 9 sites across Atlantis Massif ocean core complex (Mid-Atlantic Ridge 30°N). The goals of this expedition were to investigate serpentinization processes and microbial activity in the shallow subsurface of highly altered ultramafic and mafic sequences that have been uplifted to the seafloor along a major detachment fault zone. More than 57 m of core were recovered, with borehole penetration ranging from 1.3 to 16.4 meters below seafloor, and core recovery as high as 75% of total penetration in one borehole. The cores show highly heterogeneous rock types and alteration associated with changes in bulk rock chemistry that reflect multiple phases of magmatism, fluid-rock interaction and mass transfer within the detachment fault zone. Recovered ultramafic rocks are dominated by pervasively serpentinized harzburgite with intervals of serpentinized dunite and minor pyroxenite veins; gabbroic rocks occur as melt impregnations and veins. Dolerite intrusions and basaltic rocks represent the latest magmatic activity. The proportion of mafic rocks is volumetrically less than the amount of mafic rocks recovered previously by drilling the central dome of Atlantis Massif at IODP Site U1309. This suggests a different mode of melt accumulation in the mantle peridotites at the ridge-transform intersection and/or a tectonic transposition of rock types within a complex detachment fault zone. The cores revealed a high degree of serpentinization and metasomatic alteration dominated by talc-amphibole-chlorite overprinting. Metasomatism is most prevalent at contacts between ultramafic and mafic domains (gabbroic and/or doleritic intrusions) and points to channeled fluid flow and silica mobility during exhumation along the detachment fault. The presence of the mafic lenses within the serpentinites and their alteration to mechanically weak talc, serpentine and chlorite may also be critical in the development of the detachment fault zone and may aid in continued unroofing of the upper mantle peridotite/gabbro sequences. New technologies were also developed for the seabed drills to enable biogeochemical and microbiological characterization of the environment. An in situ sensor package and water sampling system recorded real-time variations in dissolved methane, oxygen, pH, oxidation reduction potential (Eh), and temperature and during drilling and sampled bottom water after drilling. Systematic excursions in these parameters together with elevated hydrogen and methane concentrations in post-drilling fluids provide evidence for active serpentinization at all sites. In addition, chemical tracers were delivered into the drilling fluids for contamination testing, and a borehole plug system was successfully deployed at some sites for future fluid sampling. A major achievement of IODP Expedition 357 was to obtain microbiological samples along a west–east profile, which will provide a better understanding of how microbial communities evolve as ultramafic and mafic rocks are altered and emplaced on the seafloor. Strict sampling handling protocols allowed for very low limits of microbial cell detection, and our results show that the Atlantis Massif subsurface contains a relatively low density of microbial life

Stromal Fibroblasts in Digestive Cancer

The normal gastrointestinal stroma consists of extra-cellular matrix and a community of stromal cells including fibroblasts, myofibroblasts, smooth muscle cells, pericytes, endothelium and inflammatory cells. α-smooth muscle actin (α-SMA) positive stromal fibroblasts, often referred to as myofibroblasts or activated fibroblasts, are critical in the development of digestive cancer and help to create an environment that is permissive of tumor growth, angiogenesis and invasion. This review focusses on the contribution of activated fibroblasts in carcinogenesis and where possible directly applies this to, and draws on examples from, gastrointestinal cancer. In particular, the review expands on the definition, types and origins of activated fibroblasts. It examines the molecular biology of stromal fibroblasts and their contribution to the peritumoral microenvironment and concludes by exploring some of the potential clinical applications of this exciting branch of cancer research. Understanding the origin and biology of activated fibroblasts will help in the development of an integrated epithelial-stromal sequence to cancer that will ultimately inform cancer pathogenesis, natural history and future therapeutics

Fludarabine, cytarabine, granulocyte colony-stimulating factor, and idarubicin with gemtuzumab ozogamicin improves event-free survival in younger patients with newly diagnosed aml and overall survival in patients with npm1 and flt3 mutations

Purpose To determine the optimal induction chemotherapy regimen for younger adults with newly diagnosed AML without known adverse risk cytogenetics. Patients and Methods One thousand thirty-three patients were randomly assigned to intensified (fludarabine, cytarabine, granulocyte colony-stimulating factor, and idarubicin [FLAG-Ida]) or standard (daunorubicin and Ara-C [DA]) induction chemotherapy, with one or two doses of gemtuzumab ozogamicin (GO). The primary end point was overall survival (OS). Results There was no difference in remission rate after two courses between FLAG-Ida + GO and DA + GO (complete remission [CR] + CR with incomplete hematologic recovery 93% v 91%) or in day 60 mortality (4.3% v 4.6%). There was no difference in OS (66% v 63%; P = .41); however, the risk of relapse was lower with FLAG-Ida + GO (24% v 41%; P < .001) and 3-year event-free survival was higher (57% v 45%; P < .001). In patients with an NPM1 mutation (30%), 3-year OS was significantly higher with FLAG-Ida + GO (82% v 64%; P = .005). NPM1 measurable residual disease (MRD) clearance was also greater, with 88% versus 77% becoming MRD-negative in peripheral blood after cycle 2 (P = .02). Three-year OS was also higher in patients with a FLT3 mutation (64% v 54%; P = .047). Fewer transplants were performed in patients receiving FLAG-Ida + GO (238 v 278; P = .02). There was no difference in outcome according to the number of GO doses, although NPM1 MRD clearance was higher with two doses in the DA arm. Patients with core binding factor AML treated with DA and one dose of GO had a 3-year OS of 96% with no survival benefit from FLAG-Ida + GO. Conclusion Overall, FLAG-Ida + GO significantly reduced relapse without improving OS. However, exploratory analyses show that patients with NPM1 and FLT3 mutations had substantial improvements in OS. By contrast, in patients with core binding factor AML, outcomes were excellent with DA + GO with no FLAG-Ida benefit

Hydrothermal circulation and the dike-gabbro transition in the detachment mode of slow seafloor spreading

One of the most ubiquitous boundaries within our planet is between sheeted dikes and gabbros in fast-spreading ocean crust. This boundary marks the brittle-ductile transition at the ridge crest, and is localized by a decametric conductive boundary layer between hydrothermal circulation in the sheeted dike layer and a shallow quasi-steady-state melt lens. In contrast, at slow-spreading ridges, the crustal structure appears chaotic, with no consistent sheeted dike layer and widespread occurrences of gabbro and serpentinized peridotite on the seafloor. Recent work suggests that as much as 50% of the Atlantic Ocean crust formed by a detachment mode of seafloor spreading, including the formation of oceanic core complexes capped by long-lived, convex-upward detachment faults. These detachment faults are often associated with large hydrothermal systems in which the location of any magmatic heat source is uncertain. Here we show that detachment faults can act as thermal boundaries between gabbroic melt in the fault footwall and hydrothermal circulation in the fault zone and hanging wall, thus explaining the link between faulting and black smoker systems. We suggest that interaction between magmatism and hydrothermal circulation means that detachment faults can act as the dike-gabbro transition in the detachment mode of spreading, inevitably leading to exposure of gabbros on the seafloor through continued faulting. This concept provides a means of unifying apparently contrasting processes and crustal structures at different spreading rates

Oceanic detachment faults focus very large volumes of black smoker fluids

It is generally assumed that the seawater-derived fluids that feed black smoker vent fields on the seafloor are discharged vertically from depths of 1–3 km. We present new oxygen and strontium isotope data that show that fluids at black smoker temperatures of 300–400 °C were focused along a low-angle detachment fault at 15°45'N near the Mid-Atlantic Ridge. Isotopic alteration is the most extreme ever reported from oceanic rocks altered at similar temperatures, indicating intensely focused fluid flow both in recharge and discharge parts of the hydrothermal system. Rare earth element mobility in the fault rocks demonstrates isotopic alteration by evolved hydrothermal fluids, not conductively heated seawater. The fault zone protolith was predominantly ultramafic, but also included mafic rocks, with metasomatic alteration to talc-tremolite-chlorite schists resulting mainly from chemical exchange between these lithologies during fluid flow. Fluids in equilibrium with this assemblage would be similar to ultramafic-hosted black smoker fluids. We present a new model in which hydrothermal circulation around detachment faults evolves from basalt hosted (TAG type), to footwall ultramafic hosted (Rainbow type), to low-temperature ultramafic hosted (Lost City type). Key features of our model are the intrusion of gabbro bodies immediately below the detachment to provide a heat source for circulation, and focusing of fluid flow into the detachment fault to allow venting away from the neovolcanic axis

Hess deep plutonic crust: Exploring the plutonic crust at a fast-spreading ridge: New drilling at Hess Deep

Integrated Ocean Drilling Program (IODP) Hess Deep Expedition 345 was designed to sample lower crustal primitive gabbroic rocks that formed at the fast-spreading East Pacific Rise (EPR) in order to test models of magmatic accretion and the intensity of hydrothermal cooling at depth. The Hess Deep Rift was selected to exploit tectonic exposures of young EPR plutonic crust, building upon results from ODP Leg 147 as well as more recent submersible, remotely operated vehicle, and near-bottom surveys. The primary goal was to acquire the observations required to test end-member crustal accretion models that were in large part based on relationships from ophiolites, in combination with mid-ocean ridge geophysical studies. This goal was achieved with the recovery of primitive layered olivine gabbros and troctolites with many unexpected mineralogical and textural relationships, such as the abundance of orthopyroxene and the preservation of delicate skeletal olivine textures. Site U1415 is located along the southern slope of an intrarift ridge within the Hess Deep Rift between 4675 and 4850 water depths. Specific hole locations were selected in the general area of the proposed drill sites (HD-01B-HD-03B) using a combination of geomorphology, seafloor observations, and shallow subsurface seismic data. A total of 16 holes were drilled. The primary science results were obtained from coring of two ~110 m deep reentry holes (U1415J and U1415P) and five single-bit holes (U1415E and U1415G-U1415I). Despite deep water depths and challenging drilling conditions, reasonable recovery for hard rock expeditions (15%-30%) was achieved at three 35-110 m deep holes (U1415I, U1415J, and U1415P). The other holes occupied during this expedition included two failed attempts to establish reentry capability (Holes U1415K and U1415M) and six jet-in tests to assess sediment thickness (Holes U1415A-U1415D, U1415F, and U1415L). Olivine gabbro and troctolite are the dominant plutonic rock types recovered at Site U1415, with minor gabbro, clinopyroxene oikocryst-bearing troctolite, clinopyroxene oikocryst-bearing gabbro, and gabbronorite. These rocks exhibit cumulate textures similar to those found in layered basic intrusions and some ophiolite complexes. All lithologies are primitive, with Mg# between 0.76 and 0.89, falling within the global range of primitive oceanic gabbros. Spectacular modal and/or grain size layering was prevalent in \u3e50% of the recovered core, displaying either simple or diffuse boundaries. Magmatic foliation largely defined by the shape-preferred orientation of plagioclase and olivine is moderate to strong in intervals with simple modal layering but weak to absent in the troctolite series and largely absent in the multitextured lay-ered series. The abundance of orthopyroxene in these primitive rocks was unexpected and deviates from the standard model for mid-ocean-ridge basalt crystallization. Pres-ervation of delicate skeletal olivine grains suggests that at least part of the recovered section of the lower crust was not subjected to significant hypersolidus or subsolidus strain. The metamorphic mineral assemblages record the cooling of primitive gabbroic lithologies from EPR magmatic conditions (\u3e1000°C) to zeolite facies conditions (\u3c200°C) associated with Cocos-Nazca rifting and exposure onto the seafloor. Greenschist to subgreenschist facies alteration dominates and is most pervasive in olivine-rich lithologies and within zones of brittle fracturing and cataclasis. Amphibolite facies metamorphism is not abundant in comparison to the upper gabbros at the Hess Deep Rift. The core recovered at Site U1415 originated at \u3e2 km beneath the sheeted dike-plutonic transition and thus represents the lower half to a third of the EPR plutonic crust. The orientation of the magmatic fabrics and magnetic inclinations of the core suggest that Site U1415 is composed of a series of 30-65 m thick blocks that likely formed by mass wasting. Sampling three or four blocks of relatively fresh rocks proved advantageous, as it facilitated observations of two distinct types of layering and troctolite units with varying grain size, lithologic associations, and textures. The mineralogical and textural relationships show that in several respects the Oman ophiolite is not the ideal model for fast-spreading ocean crust and call into question some aspects of both of the end-member accretion models that were to be tested. The results of the IODP Hess Deep Expedition 345 provide a reference section for primitive fast-spreading lower crust that did not exist before. This highlights the necessity of ocean drilling to address questions related to the origin and evolution of the lower ocean crust