Evolutionary potential of a dispersal-restricted species in response to climate change

Habitat replacement and fragmentation associated with projected climate change pose a critical threat to global biodiversity. Edaphically limited plant species with restricted dispersal abilities will be especially handicapped to track their optimal climate spatially. Instead, the persistence of these species will depend on their capacity to adapt in situ to novel climate regimes. Here I evaluated the evolutionary potential of Lasthenia fremontii, an annual plant species restricted to ephemeral wetlands called vernal pools in California to adapt to the projected patterns of climate change. Across L. fremontii distribution there is a latitudinal gradient in precipitation which, combined with reduced gene flow rates, might be driving adaptive divergence in climate tolerances among populations of this species. Accordingly, I estimated (1) the spatial distribution of genetic variation and gene flow across the species range, (2) the extent to which the climate variability experienced by the vernal pools has selected for seed dormancy in L. fremontii populations, and (3) the degree of local adaptation and additive genetic variation in response to a simulated spectrum of precipitation conditions. My analyses revealed an isolation-by-distance model of genetic differentiation among vernal pools and a low to moderate degree of genetic differentiation among pools within a single complex. Germination time was faster in the northernmost (historically wettest) population than in the southernmost (historically driest) population but with mixed responses in others. I observed a significant positive relationship between the historical variability in autumn precipitation and extent of seed dormancy in a population. These findings were consistent with the patterns of adaptation to local rainfall conditions observed among three of the populations reciprocally exposed to local but extreme precipitation conditions. Unexpectedly, however, populations expressed higher levels of additive genetic variation but reduced fitness under extreme drought events in comparison with moderate and extreme rainfall conditions. Further, both peripheral populations expressed optimal fitness in their native conditions but the central population did not. Taken together, these results revealed that restricted gene flow, coupled with differences in the history of local selection pressures, have led to significant divergence in the climatic tolerances and relative evolutionary potential of populations. Contrary to intuitive expectations, central range populations with less predictable climate regimes may not preserve adaptive potential for more extreme environments. That potential may only be present at the current environmental extremes

Estrés académico y ansiedad en estudiantes del décimo ciclo de enfermería de una universidad privada de Lima,2023

The objective of this study was: To analyze how academic stress is related to anxiety in tench cycle nursing students of a private university in Lima, 2023. Methodology: The method was hypothetical deductive, quantitative approach, applied type research, correlational design. The population was 123 students and the sample consisted of 93 students. The technique for data collection was the survey, and the instrument was the questionnaire. The questionnaires SISCO SV-21 for academic stress and the C. Spielberg Anxiety Rating Scale for anxiety Rating Scale for anxiety were applied. The results showed that 36.6% of students presented moderate stress with moderate anxiety; in the stressors dimension of academic stress with anxiety there is moderate stress with moderate anxiety in 31.2; in the symptoms dimension of academic stress with anxiety they presented moderate stress with moderate anxiety in 34.4%; and in the coping strategies dimension of academic stress with anxiety they presented moderate stress and moderate anxiety in 29.0%. It was concluded that academic stress and anxiety present a negative correlation (Rho= -.090) and (Sig. = .393) that is, there is no statistically significant relationship.Este estudio tuvo como Objetivo: Analizar cómo el estrés académico se relaciona con la ansiedad en estudiantes del décimo ciclo de enfermería de una universidad privada de Lima, 2023. Metodología: El método fue hipotético deductivo, enfoque cuantitativo, investigación de tipo aplicada, diseño correlacional. La Población fue 123 estudiantes y su muestra estuvo conformada por 93 estudiantes. La técnica para la recolección de datos fue la encuesta, y para instrumento fue el cuestionario. Se aplicó los cuestionarios SISCO SV-21 para el estrés académico y para la ansiedad la Escala de Valoración de la Ansiedad de C. Spielberg. Los resultados mostraron que el 36.6% de estudiantes presentaron estrés moderado con ansiedad moderado; en la dimensión estresores del estrés académico con la ansiedad existe estrés moderado con ansiedad moderado en 31.2%; en la dimensión síntomas del estrés académico con ansiedad presentaron estrés moderado con ansiedad moderado en 34.4%; y en la dimensión estrategias de afrontamiento del estrés académico con ansiedad presentaron estrés moderado y ansiedad moderado en 29.0%. Se concluyó que el estrés académico y la ansiedad presentan una correlación negativa (Rho= -.090) y (Sig. = .393), es decir no existe relación estadísticamente significativa

Desenvolupament d'una funcionalitat per validar factures energètiques de grans consumidors

Aquest projecte consisteix en la creació d'una nova funcionalitat de l'aplicació SIE (Sistema Informació Energètica), en la qual els clients que siguin grans consumidors de gas, amb un contracte indexat de gas, puguin validar les seves factures energètiques. Per poder fer aquesta validació de l'import total de consum energètic, cal simular el càlcul fet per les comercialitzadores i comprovar si l'import final reflectit en la factura és correcte. El desenvolupament del treball es divideix en tres parts principalment: obtenció de dades del mercat ibèric del gas (MIBGAS), modificació pantalla existent que permet a l'usuari introduir els preus del seu contracte (anomenada pantalla de Lots), i finalment fer el càlcul del preu amb les dades que tenim disponibles i validar si correspon amb la factura. Aquesta nova funcionalitat al SIE respon a la necessitat d'incloure els contractes indexats de gas en el sistema degut a que els clients comencen a escollir més aquest tipus de contractes per fer-li front a la pujada de preus actual de gas i electricitat.This project consists in the creation of a new funcionality of the SIE application (Energy Information System) in which customer who are large consumers of gas, with an indexed gas contract, can validate their energy bills. In order to do this validation of the total amount of energy consumption, it is necessary to simulate the calculation made by the marketers and check if the final amount reflected in the bill is correct. The development of the work is divided into three main parts: obtaining data from the Iberian gas market (MIBGAS), modification of the existing screen that allows the user to enter the prices of his contract (called Lots screen), and finally to calculate the price with the data we have available and validate if it corresponds with the invoice. This new functionality in the SIE responds to the need to include indexed gas contracts in the system due to the fact that customers are starting to choose more this type of contracts to face the current price increase in gas and electricity.Este proyecto consiste en la creación de una nueva funcionalidad de la aplicación SIE (Sistema de Información Energética) en la que los clientes que son grandes consumidores de gas, con un contrato de gas indexado, puedan validar sus facturas de energía. Para realizar esta validación del importe total del consumo de energía, es necesario simular el cálculo realizado por las comercializadoras y comprobar si el importe final reflejado en la factura es correcto. El desarrollo del trabajo se divide en tres partes principales: obtención de los datos del mercado ibérico del gas (MIBGAS), modificación de la pantalla existente que permite al usuario introducir los precios de su contrato (llamada pantalla de Lotes), y finalmente calcular el precio con los datos de los que disponemos y validar si se corresponde con la factura. Esta nueva funcionalidad en el SIE responde a la necesidad de incluir los contratos de gas indexados en el sistema debido a que los clientes están empezando a elegir más este tipo de contratos para hacer frente a la actual subida de precios del gas y la electricidad

Impacts of shrubs on soil quality in the native Monte rangelands of Southwestern Buenos Aires, Argentina

Shrub cover in semiarid rangelands may induce changes in soil resources and ecosystem functioning. However, it is unknown the real influence that shrub vegetation has on soil quality in rangelands used for livestock purposes. We evaluated the shrub cover effect on 12 chemical and biochemical parameters of soil quality. In a semiarid Monte rangeland of Argentina, 6 paddocks were selected and 10 m transects were placed in a patch with (Sh) and without shrubs (WSh). Then, sites with grasses (Sh-G and WSh-G), bare ground-litter (Sh-BL and WSh-BL), and under shrub cover (Sh-S) were selected. In spring 2017 and 2018, a composite soil sample (0–10 cm in depth) was taken at each site (n = 6). Sh-G and Sh-S sites presented high values of soil organic matter, soil organic nitrogen, particulate organic matter (POM), and cellulase activity (CA); WSh-BL and Sh-BL sites were associated with the lowest contents of these variables. For the rest of the soil quality parameters, the soil sampling sites showed similar values. These results show that woody presence should not be directly linked to soil quality loss. Although we did not detect a shrub effect in all parameters studied, in the context of appropriate grazing management, the presence of plant species of different functional groups has a positive effect on organic matter and N content of soil close to them. Moreover, in these sites, high POM values represent an important reservoir of potentially available nutrients, and promote CA necessary for fresh litter decomposition improving the soil quality of semiarid rangelands.Fil: Ambrosino, Mariela Lis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Torres, Yanina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Lucero, Cinthia Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Lorda, Graciela Susana. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Ithurrart, Leticia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Martinez, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Armando, Lorena Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Garayalde, Antonio Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Matemática; ArgentinaFil: Busso, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentin

Analytical validation of an automated assay for the measurement of adenosine deaminase (ADA) and its isoenzymes in saliva and a pilot evaluation of their changes in patients with SARS-CoV-2 infection

Objectives The aim of the present study was to validate a commercially available automated assay for the measurement of total adenosine deaminase (tADA) and its isoenzymes (ADA1 and ADA2) in saliva in a fast and accurate way, and evaluate the possible changes of these analytes in individuals with SARS-CoV-2 infection. Methods The validation, in addition to the evaluation of precision and accuracy, included the analysis of the effects of the main procedures that are currently being used for SARS-CoV-2 inactivation in saliva and a pilot study to evaluate the possible changes in salivary tADA and isoenzymes in individuals infected with SARS-CoV-2. Results The automated assay proved to be accurate and precise, with intra- and inter-assay coefficients of variation below 8.2%, linearity under dilution linear regression with R2 close to 1, and recovery percentage between 80 and 120% in all cases. This assay was affected when the sample is treated with heat or SDS for virus inactivation but tolerated Triton X-100 and NP-40. Individuals with SARS-CoV-2 infection (n=71) and who recovered from infection (n=11) had higher mean values of activity of tADA and its isoenzymes than healthy individuals (n=35). Conclusions tADA and its isoenzymes ADA1 and ADA2 can be measured accurately and precisely in saliva samples in a rapid, economical, and reproducible way and can be analyzed after chemical inactivation with Triton X-100 and NP-40. Besides, the changes observed in tADA and isoenzymes in individuals with COVID-19 open the possibility of their potential use as non-invasive biomarkers in this disease

Solucionando necesidades específicas con GNU/LINUX

Este articulo trata de la instalación, configuración y administración de una infraestructura de red basada en DMZ, para ofrecer servicios de DHCP, DNS, Controlador de Dominio, Proxy no transparente, Cortafuegos (Firewall), Servidor de archivos, Servidor de Impresión y VPN, todo lo anterior con servidores basados en GNU/Linux los cuales son Endian y Zentyal.This article deals with the installation, configuration and administration of a network infrastructure based on DMZ, to offer DHCP, DNS, Domain Controller, Non-transparent Proxy, Firewall, File Server, Print Server and VPN services, all the above with servers based on GNU/Linux which are Endian and Zentyal

Caracterización fisicoquímica de una formulación alimentaria a partir de materias primas andinas

Con el propósito de disminuir las altas cifras de desnutrición y malnutrición que se presentan en Colombia y en el mundo, se ha incursionado en el mercado de  alimentos funcionales, los cuales tienen una acción benéfica sobre la salud tanto física como mental del consumidor. Por lo anterior, el objetivo de la investigación fue evaluar el contenido de componentes mayoritarios y la viscosidad de una formulación alimentaria a partir de tres materias primas andinas como la quinua, el amaranto y el sagú. En la determinación realizada mediante los métodos de análisis de la AOAC (2005) y un viscosímetro, se encontró que al desarrollar un suplemento a base de 60% de quinua, 20% de amaranto y 20% de sagú, se obtiene un 7,03% de proteína, 5,30% de grasa, 2,05% de fibra, 2,41% de ceniza, 10,45% de agua y 72,76% de carbohidratos, y un comportamiento reológico de un fluido pseudoplástico en el que su viscosidad es independiente del tiempo pero al aumentar la velocidad ésta disminuye. Consecuentemente, es posible generar una formulación alimentaria con potencial como alimento funcional, gracias a sus propiedades nutricionales, digestivas y energéticas

Do ART and chemsex drugs get along? Potential drug-drug Interactions in a cohort of people living with HIV who engaged in chemsex: a retrospective observational study.

Introduction: People living with HIV (PLWH) who engaged in chemsex are at risk of potential drug-drug interactions (pDDIs) with recreational drugs. This study aimed to characterize pDDIs between antiretroviral treatment (ART) and chemsex drugs and evaluate their association with unscheduled relevant hospital consultations. Methods: We conducted a single-center, retrospective, observational study in a series of gay, bisexual, and other men who have sex with men (gbMSM) living with HIV who engaged in chemsex and who attended a tertiary hospital in Barcelona, Spain, from February 2018 through August 2019. Associations between all recorded pDDIs and relevant unscheduled consultations were estimated using the incidence rate (IR) per 100 person-years of those events compared between patients with no pDDI (green flag) or moderate severity pDDI (orange flag) with patients with high severity pDDI (red flag) using the incidence rate ratio (IRR). Results: Among 172 PLWH engaged in chemsex, 249 ART regimens were prescribed: 44% based on integrase inhibitors, 30% on boosted ART, and 26% based on non-nucleoside reverse transcriptase inhibitors. The substances and recreational drugs most frequently used were erectile dysfunction agents (83%), methamphetamine (79%), GHB (77%), and alkyl nitrites (71%). Polydrug use was reported in 52%. We observed 2048 pDDIs. Of these, 23% were orange flag pDDIs; 88% related to boosted ARTs. The IR of the 285 unscheduled relevant episodes in patients with orange flag pDDIs was 64.67 (95% CI 40.07-89.28). The IRR of green flag pDDIs was 1.05 (95% CI 0.60-1.8; p = 0.876). Conclusion: One in four pDDIs were of moderate severity but no significant increase in the incidence of unscheduled relevant consultations was observed. A high number of unscheduled consultations, predominantly for psychiatric events and intoxication, were observed. Beyond using non-boosted ART to minimize pDDIs, other factors related to the practice of chemsex must be addressed, in order to offer a better approach

Anti-neoplastic drugs increase caveolin-1-dependent migration, invasion and metastasis of cancer cells

Indexación: Scopus.Expression of the scaffolding protein Caveolin-1 (CAV1) enhances migration and invasion of metastatic cancer cells. Yet, CAV1 also functions as a tumor suppressor in early stages of cancer, where expression is suppressed by epigenetic mechanisms. Thus, we sought to identify stimuli/mechanisms that revert epigenetic CAV1 silencing in cancer cells and evaluate how this affects their metastatic potential. We reasoned that restricted tissue availability of anti-neoplastic drugs during chemotherapy might expose cancer cells to sub-therapeutic concentrations, which activate signaling pathways and the expression of CAV1 to favor the acquisition of more aggressive traits. Here, we used in vitro [2D, invasion] and in vivo (metastasis) assays, as well as genetic and biochemical approaches to address this question. Colon and breast cancer cells were identified where CAV1 levels were low due to epigenetic suppression and could be reverted by treatment with the methyltransferase inhibitor 5'-azacytidine. Exposure of these cells to anti-neoplastic drugs for short periods of time (24-48 h) increased CAV1 expression through ROS production and MEK/ERK activation. In colon cancer cells, increased CAV1 expression enhanced migration and invasion in vitro via pathways requiring Src-family kinases, as well as Rac-1 activity. Finally, elevated CAV1 expression in colon cancer cells following exposure in vitro to sub-cytotoxic drug concentrations increased their metastatic potential in vivo. Therefore exposure of cancer cells to anti-neoplastic drugs at non-lethal drug concentrations induces signaling events and changes in transcription that favor CAV1-dependent migration, invasion and metastasis. Importantly, this may occur in the absence of selection for drug-resistance.http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=22955&path[]=7243

Heart failure in COVID-19 patients: prevalence, incidence and prognostic implications

Aims: Data on the impact of COVID-19 in chronic heart failure (CHF) patients and its potential to trigger acute heart failure (AHF) are lacking. The aim of this work was to study characteristics, cardiovascular outcomes and mortality in patients with confirmed COVID-19 infection and a prior diagnosis of heart failure (HF). Further aims included the identification of predictors and prognostic implications for AHF decompensation during hospital admission and the determination of a potential correlation between the withdrawal of HF guideline-directed medical therapy (GDMT) and worse outcomes during hospitalization. Methods and results: Data for a total of 3080 consecutive patients with confirmed COVID-19 infection and follow-up of at least 30 days were analysed. Patients with a previous history of CHF (n = 152, 4.9%) were more prone to the development of AHF (11.2% vs. 2.1%; P < 0.001) and had higher levels of N-terminal pro brain natriuretic peptide. In addition, patients with previous CHF had higher mortality rates (48.7% vs. 19.0%; P < 0.001). In contrast, 77 patients (2.5%) were diagnosed with AHF, which in the vast majority of cases (77.9%) developed in patients without a history of HF. Arrhythmias during hospital admission and CHF were the main predictors of AHF. Patients developing AHF had significantly higher mortality (46.8% vs. 19.7%; P < 0.001). Finally, the withdrawal of beta-blockers, mineralocorticoid receptor antagonists and angiotensin-converting enzyme inhibitors or angiotensin receptor blockers was associated with a significant increase in in-hospital mortality. Conclusions: Patients with COVID-19 have a significant incidence of AHF, which is associated with very high mortality rates. Moreover, patients with a history of CHF are prone to developing acute decompensation after a COVID-19 diagnosis. The withdrawal of GDMT was associated with higher mortalit