The fate of agrochemicals in paddy rice-aquaculture systems in Northern Vietnam

During the last decades, high population growth and export-oriented economics have led to a tremendous intensification of rice production in North Vietnam, which in turn has significantly increased the amount of agrochemicals applied in rice cropping systems. Since pesticides are toxic by design, there is a natural concern on the impact of their presence in the environment on human health and environmental quality. In North Vietnam, lowland and upland paddy rice production systems were identified to be the major non-point source of agrochemical pollution to surface and ground water, which are often directly used for domestic purposes. Hence, the quantification and forecast of pesticide losses to ground and surface water from paddy rice fields is of indispensable concern and a prerequisite for assesing the potential environmental exposure and risk of water pollution across vulnerable landscapes. In Son La province, North Vietnam, paddy rice fields and fish ponds are linked, that is, irrigation water is first used in paddy fields, before it flows to the adjacent fish pond. The aim of the present study was to measure and simulate the water regime and the transport of agrochemicals in integrated farming systems in North Vietnam and to evaluate the potential risk of water pollution across the landscape. During two consecutive cropping seasons (2008) we intensively measured the water regime (inflow, outflow, water level, soil moisture) of an integrated paddy field - fish pond system and the concentrations of two applied pesticides (Dimethoate, Fenitrothion) in various system components (paddy water, soil water, pond water, soil sediment, inflow and outflow water). Pesticide samples were processed by solid phase extraction and analyzed in Hohenheim by GC and mass spectrometry. In our presentation we will focus on measurement results indicating that under the current management and pesticide application practices a considerable amount of pesticides is lost from the paddy field to the adjacent pond and further to the receiving stream and/or to the ground water

Compositional dynamics of multilevel innovation platforms in agricultural research for development

Article purchasedInnovation platforms (IPs) form a popular vehicle in agricultural research for development (AR4D) to facilitate stakeholder interaction, agenda setting, and collective action toward sustainable agricultural development. In this article, we analyze multilevel stakeholder engagement in fulfilling seven key innovation system functions. Data are gathered from experiences with interlinked community and (sub)national IPs established under a global AR4D program aimed at stimulating sustainable agricultural development in Central Africa. Our findings show that all innovation systems functions required multilevel action, but that fulfillment of specific functions demands for strategic involvement of specific stakeholders at specific levels. We observed that a research- and dissemination-oriented sequence in the functions was prioritized in AR4D IPs and argue that such a sequence may be different in other types of (business) IPs. Our findings provide an incentive to think function oriented about compositional dynamics (stakeholder groups * levels) in innovation processes, rather than striving for equal stakeholder participation

Innovation platforms: experiences with their institutional embedding in agricultural research for development

Innovation Platforms (IPs) are seen as a promising vehicle to foster a paradigm shift in agricultural research for development (AR4D). By facilitating interaction, negotiation and collective action between farmers, researchers and other stakeholders, IPs can contribute to more integrated, systemic innovation that is essential for achieving agricultural development impacts. However, successful implementation of IPs requires institutional change within AR4D establishments. The objective of this paper is to reflect on the implementation and institutionalisation of IPs in present AR4D programmes. We use experiences from sub-Saharan Africa to demonstrate how the adoption and adaptation of IPs creates both opportunities and challenges that influence platform performance and impact. Niche-regime theory is used to understand challenges, and anticipate on how to deal with them. A key concern is whether IPs in AR4D challenge or reinforce existing technology-oriented agricultural innovation paradigms. For example, stakeholder representation, facilitation and institutional embedding determine to a large extent whether the IP can strengthen systemic capacity to innovate that can lead to real paradigm change, or are merely ‘old wine in new bottles’ and a continuation of ‘business as usual’. Institutional embedding of IPs and – more broadly – the transition from technology-oriented to system-oriented AR4D approaches requires structural changes in organisational mandates, incentives, procedures and funding, as well as investments in exchange of experiences, learning and capacity development

Easing the adoption of agent-based modelling (ABM) in tourism research

Agent-based modelling (ABM) is an emerging approach in tourism research. Despite the natural fit between theories of tourism as a complex, interconnected system, and the generative approach supported in ABM, there has been only limited integration within mainstream tourism research. This research letter reports on a recent gathering of tourism ABM researchers to define the main challenges that face the adoption of ABM in tourism research. These include technical, communications, and novelty issues. In response to these challenges, three potential strategies to ease adoption are outlined: education, awareness, and interdisciplinary teams. These findings are framed as a call for increased attention to the fit of ABM within tourism research, and a framework for negotiating constraints to adoption of this technology.info:eu-repo/semantics/publishedVersio

Hypoxia induces dilated cardiomyopathy in the chick embryo: mechanism; intervention; and long-term consequences.

Background Intrauterine growth restriction is associated with an increased future risk for developing cardiovascular diseases. Hypoxia in utero is a common clinical cause of fetal growth restriction. We have previously shown that chronic hypoxia alters cardiovascular development in chick embryos. The aim of this study was to further characterize cardiac disease in hypoxic chick embryos. Methods Chick embryos were exposed to hypoxia and cardiac structure was examined by histological methods one day prior to hatching (E20) and at adulthood. Cardiac function was assessed in vivo by echocardiography and ex vivo by contractility measurements in isolated heart muscle bundles and isolated cardiomyocytes. Chick embryos were exposed to vascular endothelial growth factor (VEGF) and its scavenger soluble VEGF receptor-1 (sFlt-1) to investigate the potential role of this hypoxia-regulated cytokine. Principal Findings Growth restricted hypoxic chick embryos showed cardiomyopathy as evidenced by left ventricular (LV) dilatation, reduced ventricular wall mass and increased apoptosis. Hypoxic hearts displayed pump dysfunction with decreased LV ejection fractions, accompanied by signs of diastolic dysfunction. Cardiomyopathy caused by hypoxia persisted into adulthood. Hypoxic embryonic hearts showed increases in VEGF expression. Systemic administration of rhVEGF165 to normoxic chick embryos resulted in LV dilatation and a dose-dependent loss of LV wall mass. Lowering VEGF levels in hypoxic embryonic chick hearts by systemic administration of sFlt-1 yielded an almost complete normalization of the phenotype. Conclusions/Significance Our data show that hypoxia causes a decreased cardiac performance and cardiomyopathy in chick embryos, involving a significant VEGF-mediated component. This cardiomyopathy persists into adulthood

Hypoxia induces dilated cardiomyopathy in the chick embryo: mechanism, intervention, and long-term consequences

Background: Intrauterine growth restriction is associated with an increased future risk for developing cardiovascular diseases. Hypoxia in utero is a common clinical cause of fetal growth restriction. We have previously shown that chronic hypoxia alters cardiovascular development in chick embryos. The aim of this study was to further characterize cardiac disease in hypoxic chick embryos. Methods: Chick embryos were exposed to hypoxia and cardiac structure was examined by histological methods one day prior to hatching (E20) and at adulthood. Cardiac function was assessed in vivo by echocardiography and ex vivo by contractility measurements in isolated heart muscle bundles and isolated cardiomyocytes. Chick embryos were exposed to vascular endothelial growth factor (VEGF) and its scavenger soluble VEGF receptor-1 (sFlt-1) to investigate the potential role of this hypoxia-regulated cytokine. Principal Findings: Growth restricted hypoxic chick embryos showed cardiomyopathy as evidenced by left ventricular (LV) dilatation, reduced ventricular wall mass and increased apoptosis. Hypoxic hearts displayed pump dysfunction with decreased LV ejection fractions, accompanied by signs of diastolic dysfunction. Cardiomyopathy caused by hypoxia persisted into adulthood. Hypoxic embryonic hearts showed increases in VEGF expression. Systemic administration of rhVEGF165 to normoxic chick embryos resulted in LV dilatation and a dose-dependent loss of LV wall mass. Lowering VEGF levels in hypoxic embryonic chick hearts by systemic administration of sFlt-1 yielded an almost complete normalization of the phenotype. Conclusions/Significance: Our data show that hypoxia causes a decreased cardiac performance and cardiomyopathy in chick embryos, involving a significant VEGF-mediated component. This cardiomyopathy persists into adulthood

Comparison of Hepatic-like Cell Production from Human Embryonic Stem Cells and Adult Liver Progenitor Cells: CAR Transduction Activates a Battery of Detoxification Genes

In vitro production of human hepatocytes is of primary importance in basic research, pharmacotoxicology and biotherapy of liver diseases. We have developed a protocol of differentiation of human embryonic stem cells (ES) towards hepatocyte-like cells (ES-Hep). Using a set of human adult markers including CAAT/enhancer binding protein (C/EBPalpha), hepatocyte nuclear factor 4/7 ratio (HNF4alpha1/HNF4alpha7), cytochrome P450 7A1 (CYP7A1), CYP3A4 and constitutive androstane receptor (CAR), and fetal markers including alpha-fetoprotein, CYP3A7 and glutathione S-transferase P1, we analyzed the expression of a panel of 41 genes in ES-Hep comparatively with human adult primary hepatocytes, adult and fetal liver. The data revealed that after 21 days of differentiation, ES-Hep are representative of fetal hepatocytes at less than 20 weeks of gestation. The glucocorticoid receptor pathway was functional in ES-Hep. Extending protocols of differentiation to 4 weeks did not improve cell maturation. When compared with hepatocyte-like cells derived from adult liver non parenchymal epithelial (NPE) cells (NPE-Hep), ES-Hep expressed several adult and fetal liver makers at much greater levels (at least one order of magnitude), consistent with greater expression of liver-enriched transcription factors Forkhead box A2, C/EBPalpha, HNF4alpha and HNF6. It therefore seems that ES-Hep reach a better level of differentiation than NPE-Hep and that these cells use different lineage pathways towards the hepatic phenotype. Finally we showed that lentivirus-mediated expression of xenoreceptor CAR in ES-Hep induced the expression of several detoxification genes including CYP2B6, CYP2C9, CYP3A4, UDP-glycosyltransferase 1A1, solute carriers 21A6, as well as biotransformation of midazolam, a CYP3A4-specific substrate

The German National Registry of Primary Immunodeficiencies (2012-2017)

Introduction: The German PID-NET registry was founded in 2009, serving as the first national registry of patients with primary immunodeficiencies (PID) in Germany. It is part of the European Society for Immunodeficiencies (ESID) registry. The primary purpose of the registry is to gather data on the epidemiology, diagnostic delay, diagnosis, and treatment of PIDs. Methods: Clinical and laboratory data was collected from 2,453 patients from 36 German PID centres in an online registry. Data was analysed with the software Stata® and Excel. Results: The minimum prevalence of PID in Germany is 2.72 per 100,000 inhabitants. Among patients aged 1–25, there was a clear predominance of males. The median age of living patients ranged between 7 and 40 years, depending on the respective PID. Predominantly antibody disorders were the most prevalent group with 57% of all 2,453 PID patients (including 728 CVID patients). A gene defect was identified in 36% of patients. Familial cases were observed in 21% of patients. The age of onset for presenting symptoms ranged from birth to late adulthood (range 0–88 years). Presenting symptoms comprised infections (74%) and immune dysregulation (22%). Ninety-three patients were diagnosed without prior clinical symptoms. Regarding the general and clinical diagnostic delay, no PID had undergone a slight decrease within the last decade. However, both, SCID and hyper IgE- syndrome showed a substantial improvement in shortening the time between onset of symptoms and genetic diagnosis. Regarding treatment, 49% of all patients received immunoglobulin G (IgG) substitution (70%—subcutaneous; 29%—intravenous; 1%—unknown). Three-hundred patients underwent at least one hematopoietic stem cell transplantation (HSCT). Five patients had gene therapy. Conclusion: The German PID-NET registry is a precious tool for physicians, researchers, the pharmaceutical industry, politicians, and ultimately the patients, for whom the outcomes will eventually lead to a more timely diagnosis and better treatment