Bifidobacterial ß-galactosidase-mediated production of galacto-oligosaccharides: structural and preliminary functional assessments

This work was sponsored by FrieslandCampina. DS, VA, and FB are members of APC Microbiome Ireland, which is a research center funded by Science Foundation Ireland (SFI), through the Irish Government’s National Development Plan. The authors and their work were supported by SFI (Grant SFI/12/RC/2273), FEMS Research Grant FEMS-RG-2016-0103 and project AGL2017-84614-C2-1-R funded by the Spanish Ministry of Economy, Industry and Competitiveness. OH-H has received funding from the European Union’s Horizon 2020 Research and Innovation Program under the Marie Skłodowska- Curie grant agreement no. 843950

Influence of supplemental choline on milk yield, fatty acid profile, and weight changes in postpartum ewes and their offspring

Background and Aim: The most intensive nutritional requirements occur during milk production’s peak. Ewe milk contains more protein and fat than cow milk. The nutritional factors significantly determine the composition. The liver undergoes high stress during lactation but is relieved by essential nutrients. Choline acts metabolically as a lipotrope. This compound functions in cell structure construction, maintenance, and acetylcholine synthesis. The animal nutrition industry provides choline from various sources, such as synthetic and natural kinds. This study evaluated the influence of two distinct choline sources on dairy ewes’ peripartum and postpartum milk production, composition, and offspring growth. Materials and Methods: Twenty-four Rambouillet ewes, each weighing around 63.7 ± 1.7 kg, aged three with two previous births, spent 30-day pre-partum and post-partum in individual pens (2 × 2 m). They were given different experimental treatments 30 days before and after birth according to a randomized design; no choline (a), 4 g/day rumen-protected choline (RPC) (b), or 4 g/day thiocholine (c). Milk samples for milk composition and long-chain fatty acid (FA) analysis were taken every 30 days during milk collection. Results: Significant differences (p < 0.05) in ewe body weight, lamb birth weight, and 30-day-old lamb body weight were observed at lambing and on day 30 of lactation due to choline treatment. Milk yield was significantly higher (1.57 kg/day) compared to the control (1.02 kg/day) and RPC (1.39 kg/day), due to the herbal choline source. There was no significant difference in the milk’s protein, lactose, fat, non-fat solids, and total milk solids content between the treatments. Herbal choline lowers (p < 0.05) the concentrations of caproic, caprylic, capric, lauric, and myristic acids while boosting (p < 0.05) those of oleic and cis-11-eicosenoic acid, the changes influencing long-chain FA levels (p < 0.05). Conclusion: Providing choline from both sources to ewes enhanced milk production and body weight at lambing and on 30-day post-lambing. The herbal choline supplement altered short-chain milk FAs, while representative concentration pathways affected medium-chain ones

Complete Genome Sequence of Houston Virus, a Newly Discovered Mosquito-Specific Virus Isolated from Culex quinquefasciatus in Mexico

ABSTRACT We fully sequenced the genome of Houston virus, a recently discovered mosquito-associated virus belonging to the newly established family Mesoniviridae. The isolate was recovered from Culex quinquefasciatus in southern Mexico, which shows that the geographic range of Houston virus is not restricted to the United States in North America

A Quick Guide for Using Microsoft Onenote as an Electronic Laboratory Notebook

[Abstract] Scientific data recording and reporting systems are of a great interest for endorsing reproducibility and transparency practices among the scientific community. Current research generates large datasets that can no longer be documented using paper lab notebooks (PLNs). In this regard, electronic laboratory notebooks (ELNs) could be a promising solution to replace PLNs and promote scientific reproducibility and transparency. We previously analyzed five ELNs and performed two survey-based studies to implement an ELN in a biomedical research institute. Among the ELNs tested, we found that Microsoft OneNote presents numerous features related to ELN best functionalities. In addition, both surveyed groups preferred OneNote over a scientifically designed ELN (PerkinElmer Elements). However, OneNote remains a general note-taking application and has not been designed for scientific purposes. We therefore provide a quick guide to adapt OneNote to an ELN workflow that can also be adjusted to other nonscientific ELNs

Eduardo Torroja's Zarzuela Racecourse grandstand: Design, construction, evolution and critical assessment from the Structural Art perspective

The grandstands of La Zarzuela Racecourse in Madrid designed by the engineer E. Torroja and the architects C. Arniches and M. Dominguez are one of the world s most outstanding concrete constructions of the first half of the 20th century. This paper describes the design competition and the conceptual design process that led to their construction and carries out a critical assessment of the grandstands from the perspective of Structural Art. By doing so, the paper helps to develop the discipline of structural criticism, and points out lessons learned from their design and construction process which should not be forgotten at the present time, such as: (a) the importance of design competitions; (b) the fact that the pursuit of economy and efficiency can actually improve the quality and aesthetics of the design; (c) the importance of combining logical reasoning based on technical criteria with imagination to achieve excellence in structural design; and (d) the importance of estimating structural behavior by simple formulas and of learning from both existing structures and experimental models.The authors are very grateful to the engineers G. Ayuso and L. Fernandez Troyano from Carlos Fernandez Casado S.L. and C. Polo from the company Hipodromo de La Zarzuela S.A. for all the information and support provided. The authors also want to thank the Archivo Torroja and its former librarian, I. Garcia, for providing full access to the funds of the archive to develop this research. Funding for this research was provided by the Spanish Ministry for Science and Innovation (Research Project BIA 2011-27104).Moragues, JJ.; Paya-Zaforteza, I.; Medina, O.; Adam Martínez, JM. (2015). Eduardo Torroja's Zarzuela Racecourse grandstand: Design, construction, evolution and critical assessment from the Structural Art perspective. Engineering Structures. 105:186-196. https://doi.org/10.1016/j.engstruct.2015.10.008S18619610

Matemática para la vida, curso propedéutico

El libro aquí presentado está preparado para el desarrollo de la asignatura de Matemática para la vida del curso propedéutico que oferta la Universidad de las Regiones Autónomas de la Costa Caribe Nicaragüense (URACCAN) en sus recintos y extensiones. La motivación en la enseñanza de las matemáticas en una universidad representa una gran dificultad. La queja de muchos estudiantes es ¿por qué nos enseñan estas matemáticas que nunca las usaremos en nuestra vida diaria? Por esta razón se ha elaborado un material en el que se incorporan problemas aplicados a lo que hacemos en la vida cotidiana. Es un programa atractivo, con el cual se puede acercar esta asignatura a los estudiantes que hasta ahora habían fracasado en los estudios clásicos de Matemáticas. Es esta la razón de este libro, ya que hasta ese momento apenas había textos para el curso propedéutico, sin embargo, no se había construido un libro completo que contenga todos los elementos de la asignatura. El objetivo del libro es reforzar temas relacionados con enteros y fracciones, razones y proporciones, notación científica y conversión de unidades de medición, partiendo de aspectos históricos, comprendiendo el objeto de estudio, resolviendo ejercicios y aplicaciones relacionadas con el entorno, incidiendo de esta manera en el desarrollo de habilidades para el análisis, análisis simbólico, cálculos e interpretación de resultados. “Al igual que las ciencias, la Matemática es una especie de juego donde el universo hace de contrario. Los mejores matemáticos, y los mejores profesores de Matemáticas, son evidentemente quienes mejor comprenden las reglas del juego y gozan experimentando la emoción de jugar

USO DE MICROSOFT ONENOTE COMO CUADERNO ELECTRÓNICO DE LABORATORIO

Los sistemas de registro y de reporte de datos son de gran interés, puesto que respaldan la reproducibilidad y transparencia científica. La investigación actual genera una gran cantidad de datos que ya no se pueden documentar utilizando cuadernos de laboratorio de papel (CLP). Los cuadernos electrónicos de laboratorio (CEL) podrían ser una solu-ción prometedora para reemplazar los CLP y promover la reproducibilidad científica y su transparencia. Anteriormente analizamos cinco CEL y realizamos dos encuestas para implementar un CEL en un instituto de investigación biomédica. Entre los CEL proba-dos, encontramos que Microsoft OneNote presenta numerosas características relacio-nadas con las mejores funcionalidades del CEL. Además, ambos grupos encuestados prefirieron OneNote sobre un CEL científico (Elements de PerkinElmer). Sin embargo, OneNote es una aplicación general para tomar notas que no ha sido diseñada para fi-nes científicos. Por lo tanto, en este trabajo proporcionamos varias pautas para adaptar OneNote a un flujo de trabajo experimental

Mejora de la competitividad del sector de la dorada (Sparus aurata) a través de la selección genética. PNA PROGENSA-III

Trabajo presentado en el XVI Congreso Nacional de Acuicultura, celebrado en Zaragoza (España), del 3 al 5 de octubre de 2017[EN] This project promotes technology developments for gilthead sea bream industrial sector of Spain, to facilitate and normalize the implementation of genetic selection schemes in this species, by defining new technological traits of commercial interest, with new standardized and extensible technological developments that permit, in an economical manner, normalized measuring methods, to study genetic parameters (growth,morphology, carcass and resistance disease), genotype-environment interaction to extend the Spanish production, and guarantee the production levels to the whole production chain. At the same time, improving the breeders management under industrial conditions, the validation of feeding efficiency during the selection processes and location of biomarkers linked and applied in genomic selection.[ES] Con la presente propuesta se pretende desarrollar metodología transferible al sector industrial de dorada en España que facilite y normalice la implementación de esquemas de selección genética en esta especie, mediante la definición de caracteres de interés comercial con nuevos desarrollos tecnológicos que estandaricen y hagan extensible a todo el sector, de manera económica, la medición de caracteres a escala industrial, el estudio de parámetros genéticos para caracteres de interés comercial (crecimiento, morfología, rendimiento y resistencia a enfermedades) en stocks de reproductores de la industria española, la estimación de la interacción genotipo-ambiente para permitir de manera garante la expansión de los rendimientos productivos de la industria Española, la gestión de reproductores bajo los propios condicionantes de la industria, así como la validación de la eficiencia alimenticia de los procesos de selección y búsqueda de biomarcadores asociados y aplicables en los modelos de selección.Este trabajo ha sido financiado a través de las Ayudas a la investigación de los Planes Nacionales de Acuicultura 2016 por el Ministerio de Agricultura y Pesca, Alimentación y Medioambiente (MAPAMA) y el Fondo Europeo Marítimo y de Pesca (FEMP).Peer reviewe