Exploration von sozialen Netzwerken im 3D Raum am Beispiel von SONAR für Last.fm

Bluhm A, Eickmeyer J, Feith T, Mattar N, Pfeiffer T. Exploration von sozialen Netzwerken im 3D Raum am Beispiel von SONAR für Last.fm. In: Virtuelle und Erweiterte Realität: 6. Workshop der GI-Fachgruppe VR/AR. Aachen: Shaker Verlag; 2009: 269-280.Die Portale für soziale Netzwerke im Internet gehen mittlerweile deutlich über die Verwaltung einfacher Bekanntschaftsbeziehungen hinaus. Ihnen liegen immer reichhaltigere Datenmodelle zu Grunde. Darstellung und Exploration dieser Netzwerke sind eine grosse Herausforderung für die Entwickler, wenn beides nicht zu einer solchen für die Benutzer werden soll. Im Rahmen eines studentischen Projektes wurde die dritte Dimension für die Darstellung des komplexen sozialen Netzwerkes von Last.fm nutzbar gemacht. Durch die entwickelte Anwendung SoNAR wird das Netzwerk interaktiv und intuitiv sowohl am Desktop, als auch in der Immersion einer dreiseitigen CAVE explorierbar. Unterschiedliche Relationen des sozialen Graphen können parallel exploriert und damit Zusammenhänge zwischen Individuen intuitiv erfahren werden. Eine Suchfunktion erlaubt dabei die fexible Komposition verschiedener Startknoten für die Exploration

The eHumanities Desktop - An Online System for Corpus Management and Analysis in Support of Computing in the Humanities

Gleim R, Waltinger U, Ernst A, Mehler A, Esch D, Feith T. The eHumanities Desktop - An Online System for Corpus Management and Analysis in Support of Computing in the Humanities. In: Proceedings of the Demonstrations Session of the 12th Conference of the European Chapter of the Association for Computational Linguistics EACL 2009. 2009

eHumanities Desktop - eine webbasierte Arbeitsumgebung für die geistes-wissenschaftliche Fachinformatik

Mehler A, Gleim R, Waltinger U, Ernst A, Esch D, Feith T. eHumanities Desktop - eine webbasierte Arbeitsumgebung für die geistes-wissenschaftliche Fachinformatik. In: Proceedings of the Symposium Sprachtechnologie und eHumanities, 26. and 27. February, Duisburg-Essen University. 2009

Metabolic engineering to guide evolution - Creating a novel mode for L-valine production with Corynebacterium glutamicum

Schwentner A, Feith A, Muench E, et al. Metabolic engineering to guide evolution - Creating a novel mode for L-valine production with Corynebacterium glutamicum. METABOLIC ENGINEERING. 2018;47:31-41.Evolutionary approaches are often undirected and mutagen-based yielding numerous mutations, which need elaborate screenings to identify relevant targets. We here apply Metabolic engineering to Guide Evolution (MGE), an evolutionary approach evolving and identifying new targets to improve microbial producer strains. MGE is based on the idea to impair the cell's metabolism by metabolic engineering, thereby generating guided evolutionary pressure. It consists of three distinct phases: (i) metabolic engineering to create the evolutionary pressure on the applied strain followed by (ii) a cultivation phase with growth as straightforward screening indicator for the evolutionary event, and (iii) comparative whole genome sequencing (WGS), to identify mutations in the evolved strains, which are eventually re-engineered for verification. Applying MGE, we evolved the PEP and pyruvate carboxylase-deficient strain C. glutamicum Delta ppc Delta pyc to grow on glucose as substrate with rates up to 0.31 +/- 0.02 h(-1) which corresponds to 80% of the growth rate of the wildtype strain. The intersection of the mutations identified by WGS revealed isocitrate dehydrogenase (ICD) as consistent target in three independently evolved mutants. Upon re-engineering in C. glutamicum Delta ppc Delta pyc, the identified mutations led to diminished ICD activities and activated the glyoxylate shunt replenishing oxaloacetate required for growth. Intracellular relative quantitative metabolome analysis showed that the pools of citrate, isocitrate, cis-aconitate, and L-valine were significantly higher compared to the WT control. As an alternative to existing L-valine producer strains based on inactivated or attenuated pyruvate dehydrogenase complex, we finally engineered the PEP and pyruvate carboxylase-deficient C. glutamicum strains with identified ICD mutations for L-valine production by overexpression of the L-valine biosynthesis genes. Among them, C. glutamicum Delta ppc Delta pyc ICDG407S (pJC4ilvBNCE) produced up to 8.9 +/- 0.4 g L-valine L-1, with a product yield of 0.22 +/- 0.01 g L-valine per g glucose

Revisiting the Growth Modulon of Corynebacterium glutamicum Under Glucose Limited Chemostat Conditions.

Graf M, Haas T, Teleki A, et al. Revisiting the Growth Modulon of Corynebacterium glutamicum Under Glucose Limited Chemostat Conditions. Frontiers in Bioengineering and Biotechnology. 2020;8: 584614.Increasing the growth rate of the industrial host Corynebacterium glutamicum is a promising target to rise productivities of growth coupled product formation. As a prerequisite, detailed knowledge about the tight regulation network is necessary for identifying promising metabolic engineering goals. Here, we present comprehensive metabolic and transcriptional analysis of C. glutamicum ATCC 13032 growing under glucose limited chemostat conditions with mu = 0.2, 0.3, and 0.4 h-1. Intermediates of central metabolism mostly showed rising pool sizes with increasing growth. 13C-metabolic flux analysis (13C-MFA) underlined the fundamental role of central metabolism for the supply of precursors, redox, and energy equivalents. Global, growth-associated, concerted transcriptional patterns were not detected giving rise to the conclusion that glycolysis, pentose-phosphate pathway, and citric acid cycle are predominately metabolically controlled under glucose-limiting chemostat conditions. However, evidence is found that transcriptional regulation takes control over glycolysis once glucose-rich growth conditions are installed. Copyright © 2020 Graf, Haas, Teleki, Feith, Cerff, Wiechert, Noh, Busche, Kalinowski and Takors