Quantitative analysis of chromatin compaction in living cells using FLIM-FRET

FRET analysis of cell lines expressing fluorescently tagged histones on separate nucleosomes demonstrates that variations in chromosome compaction occur during mitosis

Landscape-scale benefits of protected areas for tropical biodiversity

We are indebted to numerous local communities, PA and government agency staff, research assistants, and other partners for supporting the field data collection. Research permissions were granted by appropriate forestry and conservation government departments in each country. Special thanks is given to the Sarawak State Government, Sarawak Forestry Corporation, Forest Department Sarawak, Sabah Biodiversity Centre, the Danum Valley Management Committee, the Forest Research Institute Malaysia (FRIM), the Smithsonian Institute and the Tropical Ecology Assessment and Monitoring (TEAM) network, Sarayudh Bunyavejchewin, and Ronglarp Sukmasuang. Support was provided by the United Nations Development Programme, NASA grants NNL15AA03C and 80NSSC21K0189, National Geographic Society’s Committee for the Research and Exploration award #9384–13, the Australian Research Council Discovery Early Career Researcher Award DECRA #DE210101440, the Universiti Malaysia Sarawak, the Ministry of Higher Education Malaysia, Nanyang Technological University Singapore, the Darwin Initiative, Liebniz-IZW, and the Universities of Aberdeen, British Columbia, Montana, and Queensland.Peer reviewedPostprin

COVID-19 and Thrombotic or Thromboembolic Disease: Implications for Prevention, Antithrombotic Therapy, and Follow-up

Coronavirus disease 2019 (COVID-19), a viral respiratory illness caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), may predispose patients to thrombotic disease, both in the venous and arterial circulations, due to excessive inflammation, platelet activation, endothelial dysfunction, and stasis. In addition, many patients receiving antithrombotic therapy for thrombotic disease may develop COVID-19, which can have implications for choice, dosing, and laboratory monitoring of antithrombotic therapy. Moreover, during a time with much focus on COVID-19, it is critical to consider how to optimize the available technology to care for patients without COVID-19 who have thrombotic disease. Herein, we review the current understanding of the pathogenesis, epidemiology, management and outcomes of patients with COVID-19 who develop venous or arterial thrombosis, and of those with preexisting thrombotic disease who develop COVID-19, or those who need prevention or care for their thrombotic disease during the COVID-19 pandemic.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/155446/1/Bikdeli-2020-COVID-19 and Thrombotic or Thromb.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/155446/3/DeepBluepermissions_agreement-CCBYandCCBY-NC_ORCID_Barnes.docxhttps://deepblue.lib.umich.edu/bitstream/2027.42/155446/4/license_rdf.rdfDescription of Bikdeli-2020-COVID-19 and Thrombotic or Thromb.pdf : ArticleDescription of DeepBluepermissions_agreement-CCBYandCCBY-NC_ORCID_Barnes.docx : Deep Blue sharing agreemen

Lattice QCD and Particle Physics

Contribution from the USQCD Collaboration to the Proceedings of the US Community Study on the Future of Particle Physics (Snowmass 2021)

Discutindo a educação ambiental no cotidiano escolar: desenvolvimento de projetos na escola formação inicial e continuada de professores

A presente pesquisa buscou discutir como a Educação Ambiental (EA) vem sendo trabalhada, no Ensino Fundamental e como os docentes desta escola compreendem e vem inserindo a EA no cotidiano escolar., em uma escola estadual do município de Tangará da Serra/MT, Brasil. Para tanto, realizou-se entrevistas com os professores que fazem parte de um projeto interdisciplinar de EA na escola pesquisada. Verificou-se que o projeto da escola não vem conseguindo alcançar os objetivos propostos por: desconhecimento do mesmo, pelos professores; formação deficiente dos professores, não entendimento da EA como processo de ensino-aprendizagem, falta de recursos didáticos, planejamento inadequado das atividades. A partir dessa constatação, procurou-se debater a impossibilidade de tratar do tema fora do trabalho interdisciplinar, bem como, e principalmente, a importância de um estudo mais aprofundado de EA, vinculando teoria e prática, tanto na formação docente, como em projetos escolares, a fim de fugir do tradicional vínculo “EA e ecologia, lixo e horta”.Facultad de Humanidades y Ciencias de la Educació

% Closed Forest 16km-radius Window

Using Hansen et al.’s (2013) original percent forest cover layer (http://earthenginepartners.appspot.com/science-2013-global-forest/download_v1.2.html), we reclassified >40% forest cover as ‘closed forest’. We then used FRAGSTATS (McGarigal, Cushman, Neel, & Ene, 2012) to calculate the percentage of the landscape occupied by closed forest habitat within a 16km-radius moving window. The data is in raster .tif format for use in GIS. We resampled the source data from its native 30m resolution to 250m resolution. Coordinate system: Asia South Albers Equal Area Conic

Mean Annual Precipitation Focal Mean 32km-radius Window

Data for this GIS raster layer was obtained from the WorldClim Global Climate Data Portal (http://worldclim.org/version2). We then used neighborhood statistics in ArcGIS to calculate the focal mean of MAP within a 32km-radius moving window. We re-sampled the source data from its native 30-arcsec resolution (~1km) to 250m. Units are in millimeters of precipitation. Coordinate system: Asia South Albers Equal Area Conic

Shrubland/Grassland Correlation Length 16km-radius Window

Original data for this GIS raster layer was obtained from the ESA CCI Land Cover Layer 300m 2015 (www.esa-landcover-cci.org). We first reclassified the original data layer, denoting shrubland/grassland as present (1) or absent (0). We then used FRAGSTATS (McGarigal, Cushman, Neel, & Ene, 2012) to calculate the correlation length or area-weighted mean of gyration within a 16km-radius moving window. Correlation length measures the average distance an individual can travel within a habitat patch, or in this case the average extensiveness of shrubland/grassland habitat within a 16km-radius window. We re-sampled the source data from its native 300m resolution to 250m. Units are in meters. Coordinate system: Asia South Albers Equal Area Conic

Compound Topographic Index Focal Mean 500m-radius Window

This GIS raster was derived from Jarvis, Reuter, Nelson, & Guevara's (2008) digital elevation model (http://srtm.csi.cgiar.org) using the Geomorphometry & Gradient Metrics Toolbox (Evans, Oakleaf, Cushman, & Theobald, 2014) in ArcGIS 10.2.2 (ESRI, Redlands, CA, USA, 2011). Compound topographic index (CTI) characterizes flow accumulation. Low elevation drainages incur high CTI, whereas mountaintops and ridgelines exhibit low CTI. We then used neighborhood statistics in ArcGIS to calculate the focal mean within a 500m-radius moving window. We re-sampled the native 90m resolution DEM to 250m. Coordinate system: Asia South Albers Equal Area Conic