A systematic study of the connection between white dwarf period spectra and model structure

To date, pulsational variability has been measured from nearly 70 DBVs and 500 DAVs, with only a fraction of these having been the subjects of asteroseismic analysis. One way to approach white dwarf asteroseismology is forward modeling, where one assumes an interior structure and calculates the model's periods. Many such models are calculated, in the search for the one that best matches an observed period spectrum. It is not computationaly manageable, nor necessary, to vary every possible parameter for every object. We engage in a systematic study, based on a sample of 14 hydrogen atmosphere white dwarfs, chosen to be representative of the types of pulsation spectra we encounter in white dwarf asteroseismology. These white dwarfs are modeled with carbon and oxygen cores . Our goal is to draw a connection between the period spectra and what parameters they are most sensitive to. We find that the presence of longer period modes generally muddies the mass and effective temperature determinations, unless continuous sequences of l = 1 and l = 2 modes are present. All period spectra are sensitive to structure in the helium and hydrogen envelope and most to at least some fatures of the oxygen abundance profile. Such sensitivity can be achieved either by the presence of specific low radial overtone modes, or by the presence of longer period modes. Convective efficiency only matters when fitting periods greater than 800 s. The results of this study can be used to inform parameter selection and pave the way to pipeline asteroseismic fitting of white dwarfs.Comment: 17 pages, 9 figures. In press at the Ap

Fine Grid Asteroseismology Of G117-B15A And R548

We now have a good measurement of the cooling rate of G117-B15A. In the near future, we will have equally well determined cooling rates for other pulsating white dwarfs, including R548. The ability to measure their cooling rates offers us a unique way to study weakly interacting particles that would contribute to their cooling. Working toward that goal, we perform a careful asteroseismological analysis of G117-B15A and R548. We study them side by side because they have similar observed properties. We carry out a systematic, fine grid search for best-fit models to the observed period spectra of those stars. We freely vary four parameters: the effective temperature, the stellar mass, the helium layer mass, and the hydrogen layer mass. We identify and quantify a number of uncertainties associated with our models. Based on the results of that analysis and fits to the periods observed in R548 and G117-B15A, we clearly define the regions of the four-dimensional parameter space occupied by the best-fit models.NSF AST 05-07639Astronom

Asteroseismic constraints on diffusion in WD envelopes

The asteroseismic analysis of white dwarfs allows us to peer below their photospheres and determine their internal structure. At ~ 28,000 K EC20058-5234 is the hottest known pulsating helium atmosphere white dwarf. As such, it constitutes an important link in the evolution of white dwarfs down the cooling track. It is also astrophysically interesting because it is at a temperature where white dwarfs are expected to cool mainly through the emission of plasmon neutrinos. In the present work, we perform an asteroseismic analysis of EC20058-5234 and place the results in the context of stellar evolution and time dependent diffusion calculations. We use a parallel genetic algorithm complemented with targeted grid searches to find the models that fit the observed periods best. Comparing our results with similar modeling of EC20058-5234's cooler cousin CBS114, we find a helium envelope thickness consistent with time dependent diffusion calculations and obtain a precise mode identification for EC20058-5234.Comment: 6 pages, 2 figures, 7 tables, accepted for publication in MNRA

The First Three Rungs of the Cosmological Distance Ladder

It is straightforward to determine the size of the Earth and the distance to the Moon without making use of a telescope. The methods have been known since the 3rd century BC. However, few amateur or professional astronomers have worked this out from data they themselves have taken. Here we use a gnomon to determine the latitude and longitude of South Bend, Indiana, and College Station, Texas, and determine a value of the radius of the Earth of 6290 km, only 1.4 percent smaller than the true value. We use the method of Aristarchus and the size of the Earth's shadow during the lunar eclipse of 2011 June 15 to derive an estimate of the distance to the Moon (62.3 R_Earth), some 3.3 percent greater than the true mean value. We use measurements of the angular motion of the Moon against the background stars over the course of two nights, using a simple cross staff device, to estimate the Moon's distance at perigee and apogee. Finally, we use simultaneous CCD observations of asteroid 1996 HW1 obtained with small telescopes in Socorro, New Mexico, and Ojai, California, to derive a value of the Astronomical Unit of (1.59 +/- 0.19) X 10^8 km, about 6 percent too large. The data and methods presented here can easily become part of a beginning astronomy lab class.Comment: 34 pages, 11 figures, accepted for publication in American Journal of Physic

Measuring The Evolutionary Rate Of Cooling Of ZZ Ceti

We have finally measured the evolutionary rate of cooling of the pulsating hydrogen atmosphere (DA) white dwarf ZZ Ceti (Ross 548), as reflected by the drift rate of the 213.13260694 s period. Using 41 yr of time-series photometry from 1970 November to 2012 January, we determine the rate of change of this period with time to be dP/dt = (5.2 +/- 1.4) x 10(-15) s s(-1) employing the O - C method and (5.45 +/- 0.79) x 10(-15) s s(-1) using a direct nonlinear least squares fit to the entire lightcurve. We adopt the dP/dt obtained from the nonlinear least squares program as our final determination, but augment the corresponding uncertainty to a more realistic value, ultimately arriving at the measurement of dP/dt = (5.5 +/- 1.0) x 10(-15) s s(-1). After correcting for proper motion, the evolutionary rate of cooling of ZZ Ceti is computed to be (3.3 +/- 1.1) x 10(-15) s s(-1). This value is consistent within uncertainties with the measurement of (4.19 +/- 0.73) x 10(-15) s s(-1) for another similar pulsating DA white dwarf, G 117-B15A. Measuring the cooling rate of ZZ Ceti helps us refine our stellar structure and evolutionary models, as cooling depends mainly on the core composition and stellar mass. Calibrating white dwarf cooling curves with this measurement will reduce the theoretical uncertainties involved in white dwarf cosmochronometry. Should the 213.13 s period be trapped in the hydrogen envelope, then our determination of its drift rate compared to the expected evolutionary rate suggests an additional source of stellar cooling. Attributing the excess cooling to the emission of axions imposes a constraint on the mass of the hypothetical axion particle.NSF AST-1008734, AST-0909107Norman Hackerman Advanced Research Program 003658-0252-2009Astronom

Measuring The Evolutionary Rate Of Cooling Of ZZ Ceti

We have finally measured the evolutionary rate of cooling of the pulsating hydrogen atmosphere (DA) white dwarf ZZ Ceti (Ross 548), as reflected by the drift rate of the 213.13260694 s period. Using 41 yr of time-series photometry from 1970 November to 2012 January, we determine the rate of change of this period with time to be dP/dt = (5.2 +/- 1.4) x 10(-15) s s(-1) employing the O - C method and (5.45 +/- 0.79) x 10(-15) s s(-1) using a direct nonlinear least squares fit to the entire lightcurve. We adopt the dP/dt obtained from the nonlinear least squares program as our final determination, but augment the corresponding uncertainty to a more realistic value, ultimately arriving at the measurement of dP/dt = (5.5 +/- 1.0) x 10(-15) s s(-1). After correcting for proper motion, the evolutionary rate of cooling of ZZ Ceti is computed to be (3.3 +/- 1.1) x 10(-15) s s(-1). This value is consistent within uncertainties with the measurement of (4.19 +/- 0.73) x 10(-15) s s(-1) for another similar pulsating DA white dwarf, G 117-B15A. Measuring the cooling rate of ZZ Ceti helps us refine our stellar structure and evolutionary models, as cooling depends mainly on the core composition and stellar mass. Calibrating white dwarf cooling curves with this measurement will reduce the theoretical uncertainties involved in white dwarf cosmochronometry. Should the 213.13 s period be trapped in the hydrogen envelope, then our determination of its drift rate compared to the expected evolutionary rate suggests an additional source of stellar cooling. Attributing the excess cooling to the emission of axions imposes a constraint on the mass of the hypothetical axion particle.NSF AST-1008734, AST-0909107Norman Hackerman Advanced Research Program 003658-0252-2009Astronom

Consensus guidelines for the use and interpretation of angiogenesis assays

The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation. As such, this collaborative work is the first edition of consensus guidelines on angiogenesis bioassays to serve for current and future reference

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival