Arquitectura orientada a objetos para aplicaciones sensibles al contexto

Esta tesis abarca dos conceptos importantes: por un lado el relacionado con los dispositivos móviles, los cuales son básicamente dispositivos con ciertas capacidades de cómputo y conexión, que pueden ser llevados por los usuarios más allá de sus estaciones de trabajo; y por otro lado, se hallan las aplicaciones que aumentan sus prestaciones al incorporar información acerca del "contexto" o "ambiente"; en el cual se están ejecutando.Facultad de Informátic

Once upon a time… a face to face classroom. About platforms and flipped virtual classroom in Covid`s time

Cuando inició el ciclo lectivo 2020 los docentes teníamos aprobadas nuestras planificaciones diseñadas en torno al aula presencial, sin embargo, podríamos decir que, el Covid hackeo el sistema y nos puso a todos, independientemente del nivel educativo en el que enseñáramos, en una situación de virtualidad y en muchos casos de vulnerabilidad. En este trabajo se presentan las estrategias pedagógicas y el diseño curricular de la materia de grado “Bases de Datos 1”, de la Facultad de Informática de la UNLP, implementados durante esta coyuntura. Para ello, se abordan temáticas como uso de Plataformas, Aula Virtual Invertida, Aprendizaje Colaborativo y Aprendizaje Basado en Juegos. Durante el curso se desarrollaron encuestas y sondeos con los cursantes, con el objetivo de relevar sus necesidades, percepciones respecto a las propuestas y sugerencias para implementar mejoras que propiciaran su Aprendizaje Significativo y la apropiación de los conocimientos. En base a la información relevada, se espera abrir un espacio de debate con otros docentes, acerca de cómo estas nuevas prácticas adoptadas durante la no presencialidad, pueden sumarse a la planificación a futuro más allá del regreso físico a las aulas y para discutir acerca de la “nueva normalidad”, al menos en la Educación Superior.When the 2020 school year began, teachers had already approved educational planning designed around the face- to-face classroom, however, we could say that the Covid hacked the system and put us all, regardless of the educational level at which we teach, in a situation of virtuality and in many cases of vulnerability. This paper presents the pedagogical strategies and the curricular design of an undergraduate course on "Databases 1", from the “Facultad de Informática” of the UNLP implemented during this context. For this, topics such as the use of Platforms in Education, Inverted Virtual Classroom, Collaborative Learning and Game-Based Learning are addressed. During the course, surveys and opinion poll were carried out with the students, with the aim of assessing their needs, perceptions regarding the proposals that were presented and suggestions to propose improvements that would promote meaningful learning and the appropriation of knowledge by the students. themselves. Based on the information gathered, it is expected to open a space for debate with other teachers about how these new practices, adopted during the absence of the face-to-face- classroom, can be added to future planning beyond the physical return to classrooms to discuss about the "new normality" almost on Higher Education.Facultad de Informátic

Érase una vez…un aula presencial: acerca de plataformas y aulas virtuales invertidas en tiempos de Covid

Cuando inició el ciclo lectivo 2020 los docentes teníamos aprobadas nuestras planificaciones diseñadas en torno al aula presencial, sin embargo, podríamos decir que, el Covid hackeo el sistema y nos puso a todos, independientemente del nivel educativo en el que enseñáramos, en una situación de virtualidad y en muchos casos de vulnerabilidad. En este trabajo se presentan las estrategias pedagógicas y el diseño curricular de la materia de grado “Bases de Datos 1”, de la Facultad de Informática de la UNLP, implementados durante esta coyuntura. Para ello, se abordan temáticas como uso de Plataformas, Aula Virtual Invertida, Aprendizaje Colaborativo y Aprendizaje Basado en Juegos. Durante el curso se desarrollaron encuestas y sondeos con los cursantes, con el objetivo de relevar sus necesidades, percepciones respecto a las propuestas y sugerencias para implementar mejoras que propiciaran su Aprendizaje Significativo y la apropiación de los conocimientos. En base a la información relevada, se espera abrir un espacio de debate con otros docentes, acerca de cómo estas nuevas prácticas adoptadas durante la no presencialidad, pueden sumarse a la planificación a futuro más allá del regreso físico a las aulas y para discutir acerca de la “nueva normalidad”, al menos en la Educación Superior.When the 2020 school year began, teachers had already approved educational planning designed around the face- to-face classroom, however, we could say that the Covid hacked the system and put us all, regardless of the educational level at which we teach, in a situation of virtuality and in many cases of vulnerability. This paper presents the pedagogical strategies and the curricular design of an undergraduate course on Databases 1 , from the “Facultad de Informática” of the UNLP implemented during this context. For this, topics such as the use of Platforms in Education, Inverted Virtual Classroom, Collaborative Learning and Game-Based Learning are addressed. During the course, surveys and opinion poll were carried out with the students, with the aim of assessing their needs, perceptions regarding the proposals that were presented and suggestions to propose improvements that would promote meaningful learning and the appropriation of knowledge by the students. themselves. Based on the information gathered, it is expected to open a space for debate with other teachers about how these new practices, adopted during the absence of the face-to-face- classroom, can be added to future planning beyond the physical return to classrooms to discuss about the new normality almost on Higher Education

Una arquitectura orientada a objetos para Sistemas de Información Geográfica

Los SIG (Sistemas de Información Geográfica) son sistemas que se utilizan para almacenar y manipular información geográfica. Se diseñan para la recolección, almacenamiento, y análisis de objetos y fenómenos donde la posición geográfica es una de las características más importante o crítica para el análisis. En este trabajo presentamos una arquitectura orientada a objetos que enfoca los aspectos m¡is relevantes involucrados en la mayoría de las aplicaciones GIS. En particular nos referimos a los siguientes aspectos: georeferenciamiento, topologías, campos continuos y visualización.Eje: Ingeniería de software y base de datosRed de Universidades con Carreras en Informática (RedUNCI

Una arquitectura orientada a objetos para Sistemas de Información Geográfica

Los SIG (Sistemas de Información Geográfica) son sistemas que se utilizan para almacenar y manipular información geográfica. Se diseñan para la recolección, almacenamiento, y análisis de objetos y fenómenos donde la posición geográfica es una de las características más importante o crítica para el análisis. En este trabajo presentamos una arquitectura orientada a objetos que enfoca los aspectos m¡is relevantes involucrados en la mayoría de las aplicaciones GIS. En particular nos referimos a los siguientes aspectos: georeferenciamiento, topologías, campos continuos y visualización.Eje: Ingeniería de software y base de datosRed de Universidades con Carreras en Informática (RedUNCI

Identification of ZBTB18 as a novel colorectal tumor suppressor gene through genome-wide promoter hypermethylation analysis

Background Cancer initiation and progression are driven by genetic and epigenetic changes. Although genome/exome sequencing has significantly contributed to the characterization of the genetic driver alterations, further investigation is required to systematically identify cancer driver genes regulated by promoter hypermethylation. Results Using genome-wide analysis of promoter methylation in 45 colorectal cancer cell lines, we found that higher overall methylation levels were associated with microsatellite instability (MSI), faster proliferation and absence of APC mutations. Because epigenetically silenced genes could represent important oncogenic drivers, we used mRNA expression profiling of colorectal cancer cell lines and primary tumors to identify a subset of 382 (3.9%) genes for which promoter methylation was negatively associated with gene expression. Remarkably, a significant enrichment in zinc finger proteins was observed, including the transcriptional repressor ZBTB18. Re-introduction of ZBTB18 in colon cancer cells significantly reduced proliferation in vitro and in a subcutaneous xenograft mouse model. Moreover, immunohistochemical analysis revealed that ZBTB18 is frequently lost or reduced in colorectal tumors, and reduced ZBTB18 expression was found to be associated with lymph node metastasis and shorter survival of patients with locally advanced colorectal cancer. Conclusions We identified a set of 382 genes putatively silenced by promoter methylation in colorectal cancer that could significantly contribute to the oncogenic process. Moreover, as a proof of concept, we demonstrate that the epigenetically silenced gene ZBTB18 has tumor suppressor activity and is a novel prognostic marker for patients with locally advanced colorectal cancer.Peer reviewe

Investigation of the role of tyrosine kinase receptor EPHA3 in colorectal cancer

EPH signaling deregulation has been shown to be important for colorectal carcinogenesis and genome-wide sequencing efforts have identified EPHA3 as one of the most frequently mutated genes in these tumors. However, the role of EPHA3 in colorectal cancer has not been thoroughly investigated. We show here that ectopic expression of wild type EPHA3 in colon cancer cells did not affect their growth, motility/invasion or metastatic potential in vivo. Moreover, overexpression of mutant EPHA3 or deletion of the endogenous mutant EPHA3 in colon cancer cells did not affect their growth or motility. EPHA3 inactivation in mice did not initiate the tumorigenic process in their intestine, and had no effects on tumor size/multiplicity after tumor initiation either genetically or pharmacologically. In addition, immunohistochemical analysis of EPHA3 tumor levels did not reveal associations with survival or clinicopathological features of colorectal cancer patients. In conclusion, we show that EPHA3 does not play a major role in colorectal tumorigenesis. These results significantly contribute to our understanding of the role of EPH signaling during colorectal carcinogenesis, and highlighting the need for detailed functional studies to confirm the relevance of putative cancer driver genes identified in sequencing efforts of the cancer genome

Investigation of the role of tyrosine kinase receptor EPHA3 in colorectal cancer

Tyrosine kinase; EPHA3; Colorectal cancerTirosina-cinasa; EPHA3; Càncer colorectalTirosina quinasa; EPHA3; Cáncer colorrectalEPH signaling deregulation has been shown to be important for colorectal carcinogenesis and genome-wide sequencing efforts have identified EPHA3 as one of the most frequently mutated genes in these tumors. However, the role of EPHA3 in colorectal cancer has not been thoroughly investigated. We show here that ectopic expression of wild type EPHA3 in colon cancer cells did not affect their growth, motility/invasion or metastatic potential in vivo. Moreover, overexpression of mutant EPHA3 or deletion of the endogenous mutant EPHA3 in colon cancer cells did not affect their growth or motility. EPHA3 inactivation in mice did not initiate the tumorigenic process in their intestine, and had no effects on tumor size/multiplicity after tumor initiation either genetically or pharmacologically. In addition, immunohistochemical analysis of EPHA3 tumor levels did not reveal associations with survival or clinicopathological features of colorectal cancer patients. In conclusion, we show that EPHA3 does not play a major role in colorectal tumorigenesis. These results significantly contribute to our understanding of the role of EPH signaling during colorectal carcinogenesis, and highlighting the need for detailed functional studies to confirm the relevance of putative cancer driver genes identified in sequencing efforts of the cancer genome

Mitigation of coupled wind-wave-earthquake responses of a 10 MW fixed-bottom offshore wind turbine

© 2020 Elsevier Ltd In this paper we present a study on the mitigation of dynamic responses of a 10 MW monopile offshore wind turbine under coupled wind-wave-earthquake excitations. We have developed and validated the generic seismic coupled analysis and structural control architecture tool to overcome the limitation of numerical tools when examining the wind-wave-earthquake coupling effects. We investigated the dynamic responses of a 10 MW monopile offshore wind turbine under different loading combinations and found that the earthquake loading increases the tower-top displacement and pile-cap moment by 47.6% and 95.1%, respectively, compared to the wind-wave-only condition. It is found that the earthquake-induced vibration in the fore-aft direction is mitigated by the wind and wave loadings due to the energy dissipated by the aerodynamic and hydrodynamic damping. In addition, the tower responses are dominated by the earthquake excitation. In order to alleviate the tower vibration induced by the earthquake, we implemented the structural control capability within the tool using tuned mass dampers. The tuned mass dampers with appropriately selected design parameters achieve a larger mitigation on the tower-top displacement for the earthquake-only condition compared to the coupled-loading scenario. The reason is that the tuned mass damper is only effective in mitigating tower vibration, and it is not capable of reducing the tower elastic deformation which is the major contribution of the tower displacement for the coupled-loading condition. In addition, we have found that a heavier tuned mass damper requires a lower tuned frequency to achieve a larger mitigation. A configuration for the mitigation control of the 10 MW offshore wind turbine is suggested by using a 5% mass ratio of the tuned mass damper

Mechanisms of inactivation of the tumour suppressor gene RHOA in colorectal cancer

Reduced RHOA signalling has been shown to increase the growth/metastatic potential of colorectal tumours. However, the mechanisms of inactivation of RHOA signalling in colon cancer have not been characterised. A panel of colorectal cancer cell lines and large cohorts of primary tumours were used to investigate the expression and activity of RHOA, as well as the presence of RHOA mutations/deletions and promoter methylation affecting RHOA. Changes in RHOA expression were assessed by western blotting and qPCR after modulation of microRNAs, SMAD4 and c-MYC. We show here that RHOA point mutations and promoter hypermethylation do not significantly contribute to the large variability of RHOA expression observed among colorectal tumours. However, RHOA copy number loss was observed in 16% of colorectal tumours and this was associated with reduced RHOA expression. Moreover, we show that miR-200a/b/429 downregulates RHOA in colorectal cancer cells. In addition, we found that TGF- β /SMAD4 upregulates the RHOA promoter. Conversely, RHOA expression is transcriptionally downregulated by canonical Wnt signalling through the Wnt target gene c-MYC that interferes with the binding of SP1 to the RHOA promoter in colon cancer cells. We demonstrate a complex pattern of inactivation of the tumour suppressor gene RHOA in colon cancer cells through genetic, transcriptional and post-transcriptional mechanisms