Lessons from the Abyss: Reflections on Recent Fisheries Crises in Atlantic Canada and North Norway

This paper examines some of the basic economic, political and scientific assumptions we have utilized to organize fisheries activities in the North Atlantic. In particular, we discuss and criticize our commitments to corporate economic organization, centralized administrative structures, and conventional science. In addition, we raise questions about the obligation of our respective nation-states to the coastal communities which have most directly been affected by the social policies emanating from our institutional commitments

The Display of a Tourist Nation: Canada in Government Film, 1945-1959

Between 1945 and 1959, the Canadian Government Travel Bureau experimented with the production of films to promote tourism that were shown in Canada and the US. As both propaganda and instruction, these films tell us much more than is immediately apparent, providing clues to post-war ideas about nation-building, economic reconstruction, citizenship, and the relationship between the state and consumer culture. Using established stereotypes of tourist venues and experiences, as well as creating tropes about government administration and the tourist “industry” itself, the political economy of the tourist trade was transformed in these films into a commodity for mass consumption.De 1945 à 1959, l'Office du tourisme du gouvernement canadien entreprend de produire des films de promotion touristique qui sont présentés au Canada et aux Etats-Unis. À la fois propagande et pédagogie, ces films nous en disent plus long qu il n' y paraît et donnent des indices sur les idées d'après-guerre au sujet du nationalisme, de la reconstruction économique, de la citoyenneté et du rapport entre l'Etat et la culture de consommation. Dans ces films, le recours à des expériences et à des attraits touristiques fortement stéréotypés et la création de figures de style sur l’administration gouvernementale et « l'industrie » touristique elle-même, permettent de transformer l'économie politique du commerce touristique en produit de consommation de masse

Long oligodeoxynucleotides: chemical synthesis, isolation via catching-by-polymerization, verification via sequencing, and gene expression demonstration

Long oligodeoxynucleotides (ODNs) are segments of DNAs having over one hundred nucleotides (nt). They are typically assembled using enzymatic methods such as PCR and ligation from shorter 20 to 60 nt ODNs produced by automated de novo chemical synthesis. While these methods have made many projects in areas such as synthetic biology and protein engineering possible, they have various drawbacks. For example, they cannot produce genes and genomes with long repeats and have difficulty to produce sequences containing stable secondary structures. Here, we report a direct de novo chemical synthesis of 400 nt ODNs, and their isolation from the complex reaction mixture using the catching-by-polymerization (CBP) method. To determine the authenticity of the ODNs, 399 and 401 nt ODNs were synthesized and purified with CBP. The two were joined together using Gibson assembly to give the 800 nt green fluorescent protein (GFP) gene construct. The sequence of the construct was verified via Sanger sequencing. To demonstrate the potential use of the long ODN synthesis method, the GFP gene was expressed in E. coli. The long ODN synthesis and isolation method presented here provides a pathway to the production of genes and genomes containing long repeats or stable secondary structures that cannot be produced or are highly challenging to produce using existing technologies

Critical-Sized Bone Defects: Sequence and Planning.

Bone defects associated with open fractures require a careful approach and planning. At initial presentation, an emergent irrigation and debridement is required. Immediate definitive fixation is frequently safe, with the exception of those injuries that normally require staged management or very severe type IIIB and IIIC injuries. Traumatic wounds that can be approximated primarily should be closed at the time of initial presentation. Wounds that cannot be closed should have a negative pressure wound therapy dressing applied. The need for subsequent debridements remains a clinical judgment, but all nonviable tissue should be removed before definitive coverage. Cefazolin remains the standard of care for all open fractures, and type III injuries also require gram-negative coverage. Both induced membrane technique with staged bone grafting and distraction osteogenesis are excellent options for bony reconstruction. Soft tissue coverage within 1 week of injury seems critical

Correction to: Cluster identification, selection, and description in Cluster randomized crossover trials: the PREP-IT trials

An amendment to this paper has been published and can be accessed via the original article