Concentration Inequalities for Nonautonomous Stochastic Delay Differential Equations

Altemeier D. Concentration Inequalities for Nonautonomous Stochastic Delay Differential Equations. Bielefeld: Universität Bielefeld; 2017

Bericht vom 4. VIVO-Workshop 2019

Beim 4. VIVO-Workshop 2019 an der Technischen Informationsbibliothek (TIB) wurde über das Open-Source-Forschungsinformationssystem VIVO und dessen Anwendung in verschiedenen Institutionen berichtet und diskutiert. Dabei wurden einerseits technische Lösungen vorgestellt, andererseits über Konzepte wie Profilhoheit diskutiert. Der Workshop beinhaltete eine interaktive Session, in der verschiedene Themen aus der Community diskutiert und weiterentwickelt wurden

Bericht vom 4. VIVO-Workshop 2019

Beim 4. VIVO-Workshop 2019 an der Technischen Informationsbibliothek (TIB) wurde über das Open-Source-Forschungsinformationssystem VIVO und dessen Anwendung in verschiedenen Institutionen berichtet und diskutiert. Dabei wurden einerseits technische Lösungen vorgestellt, andererseits über Konzepte wie Profilhoheit diskutiert. Der Workshop beinhaltete eine interaktive Session, in der verschiedene Themen aus der Community diskutiert und weiterentwickelt wurden

A Top-Down Approach for an Automatic Precedence Graph Construction under the Influence of High Product Variety

Abstract. This paper describes a top-down method for an automatic precedence graph construction that can cope with high variant products. The concept generates a joint precedence graph including all variants of a product directly. The graph is automatically derived from the bill of materials and buildability rules as well as existing solutions for the assignment of tasks to workstations. The presented method is very error prone and can improve the practical applicability of many assembly line balancing problems, that could not be used in practice yet

CryoGrid-PIXUL-RNA: high throughput RNA isolation platform for tissue transcript analysis

Abstract Background Disease molecular complexity requires high throughput workflows to map disease pathways through analysis of vast tissue repositories. Great progress has been made in tissue multiomics analytical technologies. To match the high throughput of these advanced analytical platforms, we have previously developed a multipurpose 96-well microplate sonicator, PIXUL, that can be used in multiple workflows to extract analytes from cultured cells and tissue fragments for various downstream molecular assays. And yet, the sample preparation devices, such as PIXUL, along with the downstream multiomics analytical capabilities have not been fully exploited to interrogate tissues because storing and sampling of such biospecimens remain, in comparison, inefficient. Results To mitigate this tissue interrogation bottleneck, we have developed a low-cost user-friendly system, CryoGrid, to catalog, cryostore and sample tissue fragments. TRIzol is widely used to isolate RNA but it is labor-intensive, hazardous, requires fume-hoods, and is an expensive reagent. Columns are also commonly used to extract RNA but they involve many steps, are prone to human errors, and are also expensive. Both TRIzol and column protocols use test tubes. We developed a microplate PIXUL-based TRIzol-free and column-free RNA isolation protocol that uses a buffer containing proteinase K (PK buffer). We have integrated the CryoGrid system with PIXUL-based PK buffer, TRIzol, and PureLink column methods to isolate RNA for gene-specific qPCR and genome-wide transcript analyses. CryoGrid-PIXUL, when integrated with either PK buffer, TRIzol or PureLink column RNA isolation protocols, yielded similar transcript profiles in frozen organs (brain, heart, kidney and liver) from a mouse model of sepsis. Conclusions RNA isolation using the CryoGrid-PIXUL system combined with the 96-well microplate PK buffer method offers an inexpensive user-friendly high throughput workflow to study transcriptional responses in tissues in health and disease as well as in therapeutic interventions

Human Recombinant Apyrase Therapy Protects Against Canine Pulmonary Ischemia-Reperfusion Injury

Abstract BACKGROUND: There is accumulating evidence that extracellular adenosine triphosphate (eATP) promotes many of the underlying mechanisms that exacerbate acute lung injury. However, much of these data are from inbred rodent models, indicating the need for further investigation in higher vertebrates to better establish clinical relevance. To this end we evaluated a human recombinant apyrase therapy in a canine warm pulmonary ischemia-reperfusion injury (IRI) model and measured eATP levels in human lung recipients with or without primary lung graft dysfunction (PGD). METHODS: Warm ischemia was induced for 90 minutes in the left lung of 14 mongrel dogs. Seven minutes after reperfusion, the apyrase APT102 (1 mg/kg, n = 7) or saline vehicle (n = 7) was injected into the pulmonary artery. Arterial blood gases were obtained every 30 minutes up to 180 minutes after reperfusion. Bronchioalveolar lavage fluid (BALF) was analyzed for eATP concentration, cellularity, and inflammatory medi