474 research outputs found
Computational Analysis of Binding of the GBD Domain of WASP to Different Binding Partners
The GTP-ase binding domain (GBD) of the signaling protein Wiskott-Aldrich Syndrome Protein
(WASP) is intrinsically disordered and mutations in it have been linked with Wiskott-Aldrich Syndrome
(WAS), an X-linked disorder characterized by thrombocytopenia, eczema and recurrent infections. Here,
we use molecular dynamics simulations and the semi-empirical GROMOS 45A3 force field to study interaction
of the GBD domain of WASP with a fragment of the protein EspFU as well as with the VCA domain
of WASP (auto-inhibited state). EspFU is secreted and used by enterohaemorrhagic Escherichia coli
to hijack eukaryotic cytoskeletal machinery, and it does so by competitively disrupting the auto-inhibitory
interaction between GBD and VCA domains of WASP. In addition, naturally occurring mutations in the
VCA domain cause different variants of WAS. Our simulations confirm that the EspFU domain binds the
GBD domain similarly to the VCA domain, which explains why these two binding partners are competitive
binders of the GBD domain. Furthermore, we propose a possible mechanism to explain the higher affinity
of EspFU for the GBD domain. Finally, we show that the mutations in the VCA domain responsible
for Wiskott-Aldrich syndrome can cause formation of β-sheets in the VCA domain. This effect, combined
with the mutation-induced rearrangement of the salt bridge network, consequently disables tight binding
between GBD and VCA domains. Overall, our results provide a microscopic, dynamic picture behind the
two main ways through which the interactions involving the GBD domain of WASP participate in different
disease processes.(doi: 10.5562/cca1806
Effect of Oxidative Damage on the Stability and Dimerization of Superoxide Dismutase 1
During their life cycle, proteins are subject to different modifications involving reactive oxygen species. Such oxidative damage to proteins may lead to the formation of insoluble aggregates and cytotoxicity and is associated with age-related disorders including neurodegenerative diseases, cancer, and diabetes. Superoxide dismutase 1 (SOD1), a key antioxidant enzyme in human cells, is particularly susceptible to such modifications. Moreover, this homodimeric metalloenzyme has been directly linked to both familial and sporadic amyotrophic lateral sclerosis (ALS), a devastating, late-onset motor neuronal disease, with more than 150 ALS-related mutations in the SOD1 gene. Importantly, oxidatively damaged SOD1 aggregates have been observed in both familial and sporadic forms of the disease. However, the molecular mechanisms as well as potential implications of oxidative stress in SOD1-induced cytotoxicity remain elusive. In this study, we examine the effects of oxidative modification on SOD1 monomer and homodimer stability, the key molecular properties related to SOD1 aggregation. We use molecular dynamics simulations in combination with thermodynamic integration to study microscopic-level site-specific effects of oxidative "mutations" at the dimer interface, including lysine, arginine, proline and threonine carbonylation, and cysteine oxidation. Our results show that oxidative damage of even single residues at the interface may drastically destabilize the SOD1 homodimer, with several modifications exhibiting a comparable effect to that of the most drastic ALS-causing mutations known. Additionally, we show that the SOD1 monomer stability decreases upon oxidative stress, which may lead to partial local unfolding and consequently to increased aggregation propensity. Importantly, these results suggest that oxidative stress may play a key role in development of ALS, with the mutations in the SOD1 gene being an additional factor
Intrinsically Disordered Regions May Lower the Hydration Free Energy in Proteins: A Case Study of Nudix Hydrolase in the Bacterium Deinococcus radiodurans
The proteome of the radiation- and desiccation-resistant bacterium D. radiodurans features a group of proteins that contain significant intrinsically disordered regions that are not present in non-extremophile homologues. Interestingly, this group includes a number of housekeeping and repair proteins such as DNA polymerase III, nudix hydrolase and rotamase. Here, we focus on a member of the nudix hydrolase family from D. radiodurans possessing low-complexity N- and C-terminal tails, which exhibit sequence signatures of intrinsic disorder and have unknown function. The enzyme catalyzes the hydrolysis of oxidatively damaged and mutagenic nucleotides, and it is thought to play an important role in D. radiodurans during the recovery phase after exposure to ionizing radiation or desiccation. We use molecular dynamics simulations to study the dynamics of the protein, and study its hydration free energy using the GB/SA formalism. We show that the presence of disordered tails significantly decreases the hydration free energy of the whole protein. We hypothesize that the tails increase the chances of the protein to be located in the remaining water patches in the desiccated cell, where it is protected from the desiccation effects and can function normally. We extrapolate this to other intrinsically disordered regions in proteins, and propose a novel function for them: intrinsically disordered regions increase the “surface-properties” of the folded domains they are attached to, making them on the whole more hydrophilic and potentially influencing, in this way, their localization and cellular activity
Structure and dynamics of two β-peptides in solution from molecular dynamics simulations validated against experiment
We have studied two different β-peptides in methanol using explicit solvent molecular dynamics simulations and the GROMOS 53A6 force field: a heptapeptide (peptide 1) expected to form a left-handed 314-helix, and a hexapeptide (peptide 2) expected to form a β-hairpin in solution. Our analysis has focused on identifying and analyzing the stability of the dominant secondary structure conformations adopted by the peptides, as well as on comparing the experimental NOE distance upper bounds and 3J-coupling values with their counterparts calculated on the basis of the simulated ensembles. Moreover, we have critically compared the present results with the analogous results obtained with the GROMOS 45A3 (peptide 1) and 43A1 (peptide 2) force fields. We conclude that within the limits of conformational sampling employed here, the GROMOS 53A6 force field satisfactorily reproduces experimental findings regarding the behavior of short β-peptides, with accuracy that is comparable to but not exceeding that of the previous versions of the force field. GCE legend Conformational clustering analysis of the simulated ensemble of a ß-hexapeptide with two different simulation setups (a and b). The central members of all of the clusters populating more than 5% of all of the structures are shown, together with the most dominant hydrogen bonds and the corresponding percentages of cluster members containing the
Widespread autogenous mRNA–protein interactions detected by CLIP-seq
Autogenous interactions between mRNAs and the proteins they encode are implicated in cellular feedback-loop regulation, but their extent and mechanistic foundation are unclear. It was recently hypothesized that such interactions may be common, reflecting the role of intrinsic nucleobase–amino acid affinities in shaping the genetic code's structure. Here we analyze a comprehensive set of CLIP-seq experiments involving multiple protocols and report on widespread autogenous interactions across different organisms. Specifically, 230 of 341 (67%) studied RNA-binding proteins (RBPs) interact with their own mRNAs, with a heavy enrichment among high-confidence hits and a preference for coding sequence binding. We account for different confounding variables, including physical (overexpression and proximity during translation), methodological (difference in CLIP protocols, peak callers and cell types) and statistical (treatment of null backgrounds). In particular, we demonstrate a high statistical significance of autogenous interactions by sampling null distributions of fixed-margin interaction matrices. Furthermore, we study the dependence of autogenous binding on the presence of RNA-binding motifs and structured domains in RBPs. Finally, we show that intrinsic nucleobase–amino acid affinities favor co-aligned binding between mRNA coding regions and the proteins they encode. Our results suggest a central role for autogenous interactions in RBP regulation and support the possibility of a fundamental connection between coding and binding
X-ray refinement signficantly underestimates the level of microscopic heterogeneity in biomolecular crystals
Biophysical Structural Chemistr
Universality and diversity of folding mechanics for three-helix bundle proteins
In this study we evaluate, at full atomic detail, the folding processes of
two small helical proteins, the B domain of protein A and the Villin headpiece.
Folding kinetics are studied by performing a large number of ab initio Monte
Carlo folding simulations using a single transferable all-atom potential. Using
these trajectories, we examine the relaxation behavior, secondary structure
formation, and transition-state ensembles (TSEs) of the two proteins and
compare our results with experimental data and previous computational studies.
To obtain a detailed structural information on the folding dynamics viewed as
an ensemble process, we perform a clustering analysis procedure based on graph
theory. Moreover, rigorous pfold analysis is used to obtain representative
samples of the TSEs and a good quantitative agreement between experimental and
simulated Fi-values is obtained for protein A. Fi-values for Villin are also
obtained and left as predictions to be tested by future experiments. Our
analysis shows that two-helix hairpin is a common partially stable structural
motif that gets formed prior to entering the TSE in the studied proteins. These
results together with our earlier study of Engrailed Homeodomain and recent
experimental studies provide a comprehensive, atomic-level picture of folding
mechanics of three-helix bundle proteins.Comment: PNAS, in press, revised versio
Two-state folding over a weak free-energy barrier
We present a Monte Carlo study of a model protein with 54 amino acids that
folds directly to its native three-helix-bundle state without forming any
well-defined intermediate state. The free-energy barrier separating the native
and unfolded states of this protein is found to be weak, even at the folding
temperature. Nevertheless, we find that melting curves to a good approximation
can be described in terms of a simple two-state system, and that the relaxation
behavior is close to single exponential. The motion along individual reaction
coordinates is roughly diffusive on timescales beyond the reconfiguration time
for an individual helix. A simple estimate based on diffusion in a square-well
potential predicts the relaxation time within a factor of two.Comment: 22 pages, 5 figure
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