The effects of disruptive and stabilizing selection on body size in Drosophila melanogaster. III. Genetic analysis of two lines with different reactions to disruptive selection with mating of opposite extremes

A genetic analysis was made of two lines which when subjected to disruptive selection with compulsary mating of opposite extremes (D−) showed a different response viz. one, D−-1, showing predominantly an increase of environmental variance and possibly interaction variance, the other, D−-2, showing an increase of genetic variance. Crosses between extreme flies within lines revealed that D−-1 genomes from large flies are dominant to genomes from small individuals. In D−-2 the genetic variation is predominantly additive variance. Tests for dominant chromosome effect in crosses with an inbred stock with recessive markers showed clear third chromosome differences in D−-2 and not in D−-1. Chromosome exchange between extreme flies corroborated the importance of genetic differences in D−-2. A factor or complex of factors with large effect decreasing body size is located on third chromosomes from small flies in D−-2. Interaction between chromosomes has a similar magnitude in the two lines. Crowding and temperature experiments did not reveal an increased general sensitivity to environmental factors in D−-1, which was suggested by the enlarged environmental variance of this line

Метаболическая активность митохондрий корней гороха в условиях моделированной микрогравитации

Наведено дані досліджень впливу кліностатування на метаболічну активність мітохондрій, ізольованих із коренів 5-добових проростків гороху (Pisum sativum L). Встановлено збільшення швидкості окиснення в стані 3 малату+глутамату та екзогенного НАДН, а також значення коефіцієнта дихального контролю одночасно зі зниженням відношення АДФ/О порівняно з контролем. Висловлено припущення, що такі зміни характеру дихання є наслідком адаптації метаболічної системи мітохондрій до умов кліностатування.The effect of clinorotation on the respiration of mitochondria isolated from roots of pea 5-day-old seedlings has been examined. An increase in the rate of oxidation of malate and NADH in state 3 is detected. A respiratory control ratio is also increased simultaneously with a decrease in the efficiency of oxidative phosphorylation. Such character of mitochondrial respiration under simulated microgravity is supposed to be a consequence of the adaptation to these conditions

Genetic variation and quantitative trait loci associated with developmental stability and the environmental correlation between traits in maize

11 páginas, 4 figuras, 5 tablas.There is limited experimental information about the genetic basis of micro-environmental variance (VE) (developmental stability) and environmental correlations. This study, by using a population of maize recombinant inbred lines (RIL) and simple sequence repeat (SSR) polymorphic markers, aims at the following: firstly, to quantify the genetic component of the VE or developmental stability for four traits in maize and the environmental correlation between these traits, and secondly, to identify quantitative trait loci (QTLs) that influence these quantities. We found that, when estimating variances and correlations and testing their homogeneity, estimates and tests are needed that are not highly dependent on normality assumptions. There was significant variation among the RILs in VE and in the environmental correlation for some of the traits, implying genetic heterogeneity in the VE and environmental correlations. The genetic coefficient of variation of the environmental variance (GCVVE) was estimated to be 20%, which is lower than estimates obtained for other species. A few genomic regions involved in the stability of one trait or two traits were detected, and these did not have an important influence on the mean of the trait. One region that could be associated with the environmental correlations between traits was also detected.The National Plan for Research and Development of Spain (project code AGL2006-13140) is acknowledged for financial support. B. Ordas acknowledges a contract from the Spanish National Research Council (I3P Program).Peer reviewe

Induction of morphological aberrations by enzyme inhibition in Drosophila melanogaster

Zusatz zum Futter vonDrosophila melanogaster von 5-Fluoro-2-deoxyuridin oder Aminopterin induziert überzählige Skutellar- und Dorsozentralborsten sowie gekerbte Flügel. Diese Modifikationen wurden als Konsequenz von Enzymhemmung interpretiert

Nonadherence to psoriasis medication as an outcome of limited coping resources and conflicting goals: findings from a qualitative interview study with people with psoriasis

Background Medication nonadherence is known to limit the effectiveness of available therapies; however, little is known specifically about medication adherence in people with psoriasis. Medicines self‐management can feel onerous to those with dermatological conditions due to the nature of therapies prescribed and many individuals with psoriasis experience additional challenges such as physical and psychological comorbidities that place significant additional demands on individuals and may undermine adherence. Viewing nonadherence to medication as an outcome of limited personal coping resources and conflicting goals may help to explain medication nonadherence. Objectives To explore individuals’ perspectives of their psoriasis, medication and its management. Methods Twenty people with psoriasis were recruited from community samples in England and interviewed in‐depth about their perceptions of their psoriasis, medication, and adherence to medication and self‐management advice. Data were analysed using Framework Analysis. Results Participants reported that adhering to recommended treatment regimens conflicted with the management of the physical and psychological demands of living with psoriasis. Medication usage was viewed as a source of unresolved emotional distress and, for some, resulted in poor self‐reported adherence, which included medication overuse, underuse and rejection of prescribed therapies. Perceived lack of engagement by clinicians with participants’ self‐management difficulties was viewed as an additional source of stress and distress. Conclusions Adhering to medication in psoriasis can be an additional source of considerable emotional distress. We interpreted some episodes of nonadherence to psoriasis medication as rational attempts by individuals to minimize distress and to gain control over their life

Isotopic analysis of Solenopsis geminata diets in invaded Indonesian savanna.

OBJECTIVE: To examine the extent to which smokers express negative statements about quitting and the extent to which these statements influence general practitioners' (GPs') and practice nurses' (PNs') (dis)continuation of guideline-recommended smoking cessation care. METHODS: Fifty-two video-consultations were observed (GP-consultations: 2007-2008; PN-consultations: 2010-2011). Dialogues were transcribed verbatim and professionals' and patients' speech units were coded and analysed using sequential analyses (n=1424 speech units). RESULTS: GPs focused on asking about smoking (GPs: 42.4% versus PNs: 26.2%, p=0.011) and advising them to quit (GPs: 15.3% versus PNs: 3.5%, p<0.001), whereas PNs focused on assisting them with quitting (GPs: 25.4% versus PNs: 55.2%, p<0.001). Overall, patients expressed more negative statements about quitting than positive statements (negative: 25.3% versus positive: 11.9%, p<0.001), especially when PNs assessed their willingness to quit (OR 3.61, 95% CI 1.44-9.01) or assisted them with quitting (OR 2.23, 95% CI 1.43-3.48). PRACTICE IMPLICATIONS: An alternative approach to smoking cessation care is proposed in which GPs' tasks are limited to asking, advising, and arranging follow-up. This approach seems the least likely to evoke negative statements of patients about quitting during dialogues with GPs and is compatible with the tasks and skills of PNs who could, subsequently, assist smokers with quitting

Sexuality in patients with asthma and COPD

SummarySexual quality of life was examined in 55 outpatients with chronic obstructive pulmonary disease (COPD) and asthma, using disease-specific questionnaires. Compared to an age- and sex-matched norm group, male patients with COPD reported a significantly lower sexual quality of life on all dimensions of the questionnaire. Female patients with COPD reported a lower frequency of sexual intimacy and lower sexual quality of life overall. Patients with asthma reported sexual quality-of-life scores that were somewhat better than COPD patients but worse than the healthy control group. Patients reported that they did not discuss sexual quality-of-life issues with their physician. Sexuality needs to be discussed by the health care provider in the consultation in order to improve quality of life of patients with chronic respiratory disorders

Osteoarthritis: Patient Expectations about Future Pain, Stiffness and Function

Objectives The aim of the present study was to examine the difference between osteoarthritis patients’ self-reported assessments of current pain, stiffness and physical function and their expectations of these symptoms in one and five years’ time, and to determine the significant predictors of positive expectations. Methods Eighty patients completed ratings of baseline assessments and one- and five-year expectations of pain, stiffness and physical function using the Western Ontario and McMaster Osteoarthritis Index (WOMAC). Measures of illness perceptions, coping styles, health values, satisfaction, quality of life, optimism, self-esteem and moods were also collected at baseline. Agreement between patients’ current assessment and expectations were calculated using intra-class correlations (ICCs). Paired-sample t-tests were conducted to look at differences between assessments. Univariate logistic regressions were then performed to identify the variables significantly associated with positive expectations of pain, stiffness and function. Significant variables (p < 0.05) were entered into a forward stepwise multivariate logistic regression to identify unique independent predictors of positive expectations for each of the WOMAC subscales. Results Differences were found between current assessments and expectations, with the majority of patients being positive about future symptoms. There were some differences between the predictors for one- and five-year expectations, with current assessments of health status only affecting five-year expectations. Conclusions It is necessary to investigate further the variables that may contribute to positive expectations in osteoarthritis patients in order to manage the condition more effectively