Torocyte Membrane Endovesicles Induced by Octaethyleneglycol Dodecylether in Human Erythrocytes

AbstractEndovesicles induced in human erythrocytes by octaethyleneglycol dodecylether (C12E8) were studied by confocal laser scanning microscopy, using fluorescein isothiocyanate dextran as a nonspecific fluid marker. The endovesicles appeared to consist mainly of a ring-formed toroidal part joined with a central flat membrane segment. The torocyte contour length was several μm. There was usually one torocyte endovesicle per cell. The endovesicles seemed to be located near the cell surface. In sections of C12E8-treated erythrocytes transmission electron microscopy revealed the frequent occurrence of flat membrane structures with a bulby periphery, which apparently are cross sections of torocyte endovesicles. The possible physical mechanisms leading to the observed torocyte endovesicle shape are discussed. The torocyte endovesicles seem to be formed in a process in which an initially stomatocytic invagination loses volume while maintaining a large surface area. Because intercalation of C12E8 in the erythrocyte membrane induces inward membrane bending (stomatocytosis) we assume that C12E8 is preferentially located in the inner lipid layer of the erythrocyte membrane, i.e., in the outer lipid layer of the endovesicle membrane. It is suggested that local disturbances of the lipid molecules in the vicinity of the C12E8 molecules in the outer lipid layer of the endovesicle membrane form membrane inclusions with the effective shape of an inverted truncated cone. If the interaction between the inclusion and the membrane is weak, the membrane of such an endovesicle can be characterized by its negative spontaneous curvature, which may lead to a torocyte endovesicle shape with a small relative volume. Effects of a possible strong interaction between the C12E8-induced membrane inclusions and the membrane on the stability of the torocyte endovesicles are also indicated

Does Accessibility Planning address what matters? A review of current practice and practitioner perspectives

'Accessibility' has become commonplace in transport planning and as such there is a plethora of interpretations of what accessibility means, what constitutes a good measure of accessibility, and how this might be applied in practice. This paper presents an overview of approaches to measuring accessibility and presents a case study of Accessibility Planning in England — one approach to formalising the concept of accessibility. Results of semi-structured interviews with local authority officers are discussed to establish whether current approaches, allow their desired outcomes to be met. This approach demonstrates where there might be gaps between measured or modelled accessibility and the perceptions of the individuals. Findings suggest that while the process is deemed useful in raising the profile of accessibility issues, measures of accessibility do not necessarily easily translate into quantifying benefits of those improvements that are perceived by practitioners to improve accessibility and reduce transport disadvantage

The antiparasitic compound Licochalcon A is a potent echinocytogenic agent that modifies erythrocyte membrane in the concentration region where the antiplasmodial activity is observed

The well-known antiparasitic compound licochalcone A is a potent membrane-active agent that transforms normal erythrocytes into echinocytes in parallel with the inhibition of growth of Plasmodium falciparum cultures, the in vitro antiplasmodial effect apparently being an indirect effect on the host cell. In vitro experiments with synchronous cultures demonstrate that inhibition of invasion is the principal mechanism of growth inhibition. The erythrocyte membrane-modifying effect was also transiently observed in vivo in mice after intravenous administration

Geographical and temporal weighted regression (GTWR)

Both space and time are fundamental in human activities as well as in various physical processes. Spatiotemporal analysis and modeling has long been a major concern of geographical information science (GIScience), environmental science, hydrology, epidemiology, and other research areas. Although the importance of incorporating the temporal dimension into spatial analysis and modeling has been well recognized, challenges still exist given the complexity of spatiotemporal models. Of particular interest in this article is the spatiotemporal modeling of local nonstationary processes. Specifically, an extension of geographically weighted regression (GWR), geographical and temporal weighted regression (GTWR), is developed in order to account for local effects in both space and time. An efficient model calibration approach is proposed for this statistical technique. Using a 19-year set of house price data in London from 1980 to 1998, empirical results from the application of GTWR to hedonic house price modeling demonstrate the effectiveness of the proposed method and its superiority to the traditional GWR approach, highlighting the importance of temporally explicit spatial modeling

An event-based conceptual model for context-aware movement analysis

Current tracking technologies enable collection of data, describing movements of various kinds of objects, including people, animals, icebergs, vehicles, containers with goods and so on. Analysis of movement data is now a hot research topic. However, most of the suggested analysis methods deal with movement data alone. Little has been done to support the analysis of movement in its spatio-temporal context, which includes various spatial and temporal objects as well as diverse properties associated with spatial locations and time moments. Comprehensive analysis of movement requires detection and analysis of relations that occur between moving objects and elements of the context in the process of the movement. We suggest a conceptual model in which movement is considered as a combination of spatial events of diverse types and extents in space and time. Spatial and temporal relations occur between movement events and elements of the spatial and temporal contexts. The model gives a ground to a generic approach based on extraction of interesting events from trajectories and treating the events as independent objects. By means of a prototype implementation, we tested the approach on complex real data about movement of wild animals. The testing showed the validity of the approach

Distribution and Characterization of Progenitor Cells within the Human Filum Terminale

Filum terminale (FT) is a structure that is intimately associated with conus medullaris, the most caudal part of the spinal cord. It is well documented that certain regions of the adult human central nervous system contains undifferentiated, progenitor cells or multipotent precursors. The primary objective of this study was to describe the distribution and progenitor features of this cell population in humans, and to confirm their ability to differentiate within the neuroectodermal lineage.We demonstrate that neural stem/progenitor cells are present in FT obtained from patients treated for tethered cord. When human or rat FT-derived cells were cultured in defined medium, they proliferated and formed neurospheres in 13 out of 21 individuals. Cells expressing Sox2 and Musashi-1 were found to outline the central canal, and also to be distributed in islets throughout the whole FT. Following plating, the cells developed antigen profiles characteristic of astrocytes (GFAP) and neurons (β-III-tubulin). Addition of PDGF-BB directed the cells towards a neuronal fate. Moreover, the cells obtained from young donors shows higher capacity for proliferation and are easier to expand than cells derived from older donors.The identification of bona fide neural progenitor cells in FT suggests a possible role for progenitor cells in this extension of conus medullaris and may provide an additional source of such cells for possible therapeutic purposes. Filum terminale, human, progenitor cells, neuron, astrocytes, spinal cord