11 research outputs found
Diversity and enrichment of nitrite-dependent anaerobic methane oxidizing bacteria from wastewater sludge
Recently discovered microorganisms affiliated to the bacterial phylum NC10, named âCandidatus Methylomirabilis oxyferaâ, perform nitrite-dependent anaerobic methane oxidation. These microorganisms could be important players in a novel way of anaerobic wastewater treatment where ammonium and residual dissolved methane might be removed at the expense of nitrate or nitrite. To find suitable inocula for reactor startup, ten selected wastewater treatment plants (WWTPs) located in The Netherlands were screened for the endogenous presence of M. oxyfera using molecular diagnostic methods. We could identify NC10 bacteria with 98% similarity to M. oxyfera in nine out of ten WWTPs tested. Sludge from one selected WWTP was used to start a new enrichment culture of NC10 bacteria. This enrichment was monitored using specific pmoA primers and M. oxyfera cells were visualized with fluorescence oligonucleotide probes. After 112Â days, the enrichment consumed up to 0.4Â mM NO2â per day. The results of this study show that appropriate sources of biomass, enrichment strategies, and diagnostic tools existed to start and monitor pilot scale tests for the implementation of nitrite-dependent methane oxidation in wastewater treatment at ambient temperature
New Anaerobic, Ammonium-Oxidizing Community Enriched from Peat Soilâż â
Anaerobic ammonium-oxidizing (anammox) bacteria have been recognized as an important sink for fixed nitrogen and are detected in many natural environments. However, their presence in terrestrial ecosystems has long been overlooked, and their contribution to the nitrogen cycling in natural and agricultural soils is currently unknown. Here we describe the enrichment and characterization of anammox bacteria from a nitrogen-loaded peat soil. After 8 months of incubation with the natural surface water of the sampling site and increasing ammonium and nitrite concentrations, anammox cells constituted 40 to 50% of the enrichment culture. The two dominant anammox phylotypes were affiliated with âCandidatus Jettenia asiaticaâ and âCandidatus Brocadia fulgida.â The enrichment culture converted NH4+ and NO2â to N2 with the previously reported stoichiometry (1:1.27) and had a maximum specific anaerobic ammonium oxidation rate of 0.94 mmol NH4+·g (dry weight)â1·hâ1 at pH 7.1 and 32°C. The diagnostic anammox-specific lipids were detected at a concentration of 650 ng·g (dry weight)â1, and pentyl-[3]-ladderane was the most abundant ladderane lipid
Downstream targets of the Phytophthora infestans Galpha subunit PiGPA1 revealed by cDNA-AFLP
Identification and quantification of anammox bacteria in eight nitrogen removal reactors
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Institutional stimuli and individual response as explanations of turnout : the 2009 EP election
This article sets out a social psychological theory of voting behaviour that integrates stimuli from institutions; perceptions of stimuli from such institutions as the media and government; and individual predispositions to respond. It thus clarifies the confusion caused in the classic Michigan model, which treats perceptions as individual attributes without regard to institutional stimuli. Multi-level statistical analysis of turnout at the 2009 European Parliament election shows that, after controlling for individual predispositions, institutional stimuli have a substantial effect on behaviour both contextually and by shaping individual perceptions
A proteomics study of in vitro cyst germination and appressoria formation in €Phytophthora infestans€
The metagenome of the marine anammox bacterium âCandidatus Scalindua profundaâ illustrates the versatility of this globally important nitrogen cycle bacterium
Anaerobic ammonium-oxidizing (anammox) bacteria are responsible for a significant portion of the loss of fixed nitrogen from the oceans, making them important players in the global nitrogen cycle. To date, marine anammox bacteria found in marine water columns and sediments worldwide belong almost exclusively to the 'Candidatus Scalindua' species, but the molecular basis of their metabolism and competitive fitness is presently unknown. We applied community sequencing of a marine anammox enrichment culture dominated by 'Candidatus Scalindua profunda' to construct a genome assembly, which was subsequently used to analyse the most abundant gene transcripts and proteins. In the S. profunda assembly, 4756 genes were annotated, and only about half of them showed the highest identity to the only other anammox bacterium of which a metagenome assembly had been constructed so far, the freshwater 'Candidatus Kuenenia stuttgartiensis'. In total, 2016 genes of S. profunda could not be matched to the K. stuttgartiensis metagenome assembly at all, and a similar number of genes in K. stuttgartiensis could not be found in S. profunda. Most of these genes did not have a known function but 98 expressed genes could be attributed to oligopeptide transport, amino acid metabolism, use of organic acids and electron transport. On the basis of the S. profunda metagenome, and environmental metagenome data, we observed pronounced differences in the gene organization and expression of important anammox enzymes, such as hydrazine synthase (HzsAB), nitrite reductase (NirS) and inorganic nitrogen transport proteins. Adaptations of Scalindua to the substrate limitation of the ocean may include highly expressed ammonium, nitrite and oligopeptide transport systems and pathways for the transport, oxidation, and assimilation of small organic compounds that may allow a more versatile lifestyle contributing to the competitive fitness of Scalindua in the marine realm