Ultrasound-promoted Synthesis Of 3-(thiophen-2-yl)-4,5-dihydro-1h-pyrazole-1carboximidamides And Anticancer Activity Evaluation In Leukemia Cell Lines

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)3-(Thiophen-2-yl)-4,5-dihydro-1H-pyrazole-1-carboximidamides were efficiently prepared through a cyclocondensation of thiophenylchalcones with aminoguanidine hydrochloride under ultrasonic conditions in the presence of KOH and ethanol as a green solvent in short reaction times (15-35 min) and good yields (62-95%). All compounds produced were evaluated against the human Jurkat and RS4; 11 acute lymphoblastic leukemia cell lines of T- and B-cell origin, respectively, and the K562 myelogenous leukemia cell line. Six compounds presented half maximal inhibitory concentration (IC50) values around 15 mu mol L-1 and five compounds presented IC50 values around 40 mu mol L-1 for at least one of the three cell lines analyzed. One compound was not significantly cytotoxic, presenting IC50 value > 100 mu mol L- 1.282217224Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [483021/2013-0]Fundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT) [0180/12]Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [12/12802-1]Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)FAPESPCNPqConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

GEOMETRIC ANALYSIS BY CONSTRUCTAL DESIGN OF STIFFENED STEEL PLATES UNDER BENDING WITH TRANSVERSE I-SHAPED OR T-SHAPED STIFFENERS

Several stiffened plates arrangements subjected to bending were configured applying the Constructal Design Method (CDM) and solved by Finite Element Method (FEM), aiming through the Exhaustive Search (ES) technique analyze the influence of transverse I-Shaped or T-Shaped stiffeners in mechanical behavior. Considering a non-stiffened plate as reference and maintaining the total steel volume constant, a portion of the reference plate was deducted from its thickness, and transformed into stiffeners through the ???? volume fraction parameter, which represents the ratio between the steel volume of the stiffeners and the steel volume of the reference plate. Assuming ???? = 0.3, 25 plates with just I-Shaped stiffeners in longitudinal and transverse directions and 25 plates with I-Shaped stiffeners in longitudinal direction and T-Shaped stiffeners in transverse direction were proposed. The results showed that the plates with transverse T-Shaped stiffeners are more effective, reducing the maximum von Mises stress and maximum deflection, respectively, in up to more than 60% and 50% when compared with the plates with just I-Shaped stiffeners

Ultrasound-promoted Synthesis Of 3-(thiophen-2-yl)-4,5-dihydro-1h-pyrazole-1-carboximidamides And Anticancer Activity Evaluation In Leukemia Cell Lines

3-(Thiophen-2-yl)-4,5-dihydro-1H-pyrazole-1-carboximidamides were efficiently prepared through a cyclocondensation of thiophenylchalcones with aminoguanidine hydrochloride under ultrasonic conditions in the presence of KOH and ethanol as a green solvent in short reaction times (15-35 min) and good yields (62-95%). All compounds produced were evaluated against the human Jurkat and RS4;11 acute lymphoblastic leukemia cell lines of T- and B-cell origin, respectively, and the K562 myelogenous leukemia cell line. Six compounds presented half maximal inhibitory concentration (IC50) values around 15 µmol L-1 and five compounds presented IC50 values around 40 µmol L-1 for at least one of the three cell lines analyzed. One compound was not significantly cytotoxic, presenting IC50 value > 100 µmol L-1.28221722

Delayed Fluorescence by Triplet–Triplet Annihilation from Columnar Liquid Crystal Films

Delayed fluorescence (DF) by triplet–triplet annihilation (TTA) is observed in solutions of a benzoperylene-imidoester mesogen that shows a hexagonal columnar mesophase at room temperature in the neat state. A similar benzoperylene-imide with a slightly smaller HOMO–LUMO gap, that also is hexagonal columnar liquid crystalline at room temperature, does not show DF in solution, and mixtures of the two mesogens show no DF in solution either, because of collisional quenching of the excited triplet states on the imidoester by the imide. In contrast, DF by TTA from the imide but not from the imidoester is observed in condensed films of such mixtures, even though neat films of either single material are not displaying DF. In contrast to the DF from the monomeric imidoester in solution, DF of the imide occurs from dimeric aggregates in the blend films, assisted by the imidoester. Thus, the close contact of intimately stacked molecules of the two different species in the columnar mesophase leads to a unique mesophase-assisted aggregate DF. This constitutes the first observation of DF by TTA from the columnar liquid crystalline state. If the imide is dispersed in films of polybromostyrene, which provides an external heavy-atom effect facilitating triplet formation, DF is also observed. Organic light-emitting diodes (OLEDs) devices incorporating these liquid crystal molecules demonstrated high external quantum efficiency (EQE). On the basis of the literature and to the best of our knowledge, the EQE reported is the highest among nondoped solution-processed OLED devices using a columnar liquid crystal molecule as the emitting layer

Verification and Validation of a Methodology to Numerically Generate Waves Using Transient Discrete Data as Prescribed Velocity Boundary Condition

This work presents a two-dimensional numerical analysis of a wave channel and a oscillating water column (OWC) device. The main goal is to validate a methodology which uses transient velocity data as a means to impose velocity boundary condition for the generation of numerical waves. To achieve this, a numerical wave channel was simulated using regular waves with the same parameters as those used in a laboratory experiment. First, these waves were imposed as prescribed velocity boundary condition and compared with the analytical solution; then, the OWC device was inserted into the computational domain, aiming to validate this methodology. For the numerical analysis, computational fluid dynamics ANSYS Fluent software was employed, and to tackle with water–air interaction, the nonlinear multiphase model volume of fluid (VOF) was applied. Although the results obtained through the use of discrete data as velocity boundary condition presented a little disparity; in general, they showed a good agreement with laboratory experiment results. Since many studies use regular waves, there is a lack of analysis with ocean waves realistic data; thus, the proposed methodology stands out for its capacity of using realistic sea state data in numerical simulations regarding wave energy converters (WECs)

Ultrasound-Promoted Synthesis of 3-(Thiophen-2-yl)-4,5-dihydro-1H-pyrazole-1carboximidamides and Anticancer Activity Evaluation in Leukemia Cell Lines

3-(Thiophen-2-yl)-4,5-dihydro-1H-pyrazole-1-carboximidamides were efficiently prepared through a cyclocondensation of thiophenylchalcones with aminoguanidine hydrochloride under ultrasonic conditions in the presence of KOH and ethanol as a green solvent in short reaction times (15-35 min) and good yields (62-95%). All compounds produced were evaluated against the human Jurkat and RS411 acute lymphoblastic leukemia cell lines of T- and B-cell origin, respectively, and the K562 myelogenous leukemia cell line. Six compounds presented half maximal inhibitory concentration (IC50) values around 15 mu mol L-1 and five compounds presented IC50 values around 40 mu mol L-1 for at least one of the three cell lines analyzed. One compound was not significantly cytotoxic, presenting IC50 value > 100 mu mol L- 1.Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)Fundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)FAPESPCNPqFundacao Univ Fed Grande Dourados, Grp Pesquisa Sintese & Caracterizacao Mol MS, Rua Joao Rosa Goes 1761, BR-79825070 Dourados, MS, BrazilUniv Estadual Campinas, Inst Biol, Dept Genet & Biol Mol, Rua Monteiro Lobato 255, BR-13083970 Campinas, SP, BrazilCtr Infantil Boldrini, Mol Biol Lab, Rua Dr Gabriel Porto 1270, BR-13083210 Campinas, SP, BrazilUniv Fed Sao Paulo, Dept Ciencias Exatas & Terra, Rua Prof Artur Riedel 275, BR-09972270 Diadema, SP, BrazilUniv Fed Pelotas, Ctr Ciencias Quim Farmaceut & Alimentos, Lab Lipid & Bioorgan, Campus Univ, BR-96001900 Pelotas, RS, BrazilUniv Estadual Mato Grosso do Sul, Rua Emilio Mascolli 275, BR-79950000 Navirai, MS, BrazilUniv Fed Sao Paulo, Dept Ciencias Exatas & Terra, Rua Prof Artur Riedel 275, BR-09972270 Diadema, SP, BrazilCNPq: 483021/2013-0FUNDECT: 0180/12FAPESP: 12/12802-1Web of Scienc

A Orla de Atalaia em Aracaju/SE e seus equipamentos de esporte e lazer como problemática de pesquisa: levantamento e discussão dos dados

O presente estudo analisou a infraestrutura de esporte e lazer da Orla da praia de Atalaia em Aracaju/SE, entre maio e dezembro/2010, no qual se identificou as condições de manutenção, acesso e uso dos equipamentos de esporte e lazer desse espaço. Os dados indicam a predominância de equipamentos esportivos de lazer, em detrimento dos infantis e culturais; um processo gradativo de privatização de alguns equipamentos públicos; a ausência de projetos e políticas públicas de incentivo ao pleno acesso da população aos equipamentos; estrutura diversificada de suporte ao lazer, mas com deficiências em relação aos banheiros e transporte públicos e segurança no local. As conclusões indicam a necessidade do estado de Sergipe e do município de Aracaju reavaliarem e ampliarem as políticas públicas de esporte e lazer neste espaço, para garantir o pleno acesso da população local, e não apenas aos turistas e às classes mais abastadas

The Complex Puzzle of Interactions Among Functional Food, Gut Microbiota, and Colorectal Cancer

Colorectal cancer exerts a strong influence on the epidemiological panorama worldwide, and it is directly correlated to etiologic factors that are substantiated by genetic and environmental elements. This complex mixture of factors also has a relationship involving the structural dependence and composition of the gut microbiome, leading to a dysbacteriosis process that may evolve to serious modifications in the intestinal lining, eventually causing the development of a neoplasm. The gastrointestinal tract presents defense strategies and immunological properties that interfere in intestinal permeability, inhibiting the bacterial translocation, thus maintaining the integrity of intestinal homeostasis. The modulation of the intestinal microbiome and the extinction of risk factors associated with intestinal balance losses, especially of environmental factors, make cell and defense alterations impossible. This modulation may be conducted by means of functional foods in the diet, especially soluble fibers, polyunsaturated fatty acids, antioxidants and prebiotics that signal immunomodulatory effects in the intestinal microbiota, with preventive and therapeutic action for colorectal cancer. In summary, this review focuses on the importance of dietary modulation of the intestinal microbiota as an instrument for dysbacteriosis and, consequently, for the prevention of colorectal cancer, suggesting anticarcinogenic, and antiangiogenic properties. Among the intestinal modulating agents considered here are functional foods, especially flaxseed, oat and soy, composing a Bioactive Food Compound

IMPACTO DA “MÃE DE LEITE ELETRÔNICA” NO DESEMPENHO ZOOTÉCNICO DE LEITÕES LACTENTES

Com os avanços da genética na suinocultura e a aplicação de tecnologias no manejo o número de leitões nascidos, por parto, tem aumentado significativamente. Por consequência, as leitegadas raramente são uniformes e muitos leitões apresentam baixo peso ao nascimento. Pesquisas indicam que a cada leitão extra na média de nascidos acarreta uma redução de 100g no peso ao nascimento, dobrando o número de leitões que nascem com peso abaixo de 800g. O aumento da taxa de mortalidades entre os leitões com baixo peso, ainda nos primeiros dias de vida, é algo comum. Logo, torna-se essencial o emprego de alternativas que contribuam tanto para a diminuição da mortalidade pré-desmame quanto para o alcance da uniformidade da leitegada. Com o intuito de melhorar esses índices foi avaliado a influência do equipamento “mãe de leite eletrônica” sobre o desempenho zootécnico de leitões no período entre o nascimento e o desmame. O equipamento foi cedido por colaboradores da Empresa STA, de Joinville/SC. Cada lote experimental contou com dois grupos: o controle (GC) e o tratamento (GT). No GC os leitões receberam apenas aleitamento natural, fornecido pela mãe. No GT os leitões foram submetidos ao regime de aleitamento misto (natural, fornecido pela mãe; e o artificial, fornecido pelo equipamento o leite de vaca). Foram avaliadas 6 leitegadas, duas por experimento. As fêmeas eram provenientes de matrizes híbridas Landrace x Large White, entre o 3º e 6º parto. Ao todo realizaram-se 3 procedimentos experimentais. O aleitamento propiciado pelo equipamento beneficiou todas as leitegadas do GT já nos três primeiros dias de vida. Muitos leitões que apresentaram dificuldades de amamentarem-seadequadamente na mãe, pela competição da leitegada pelos tetos, conseguiram sobreviver pela disponibilidade de leite oferecida artificialmente. Por ocasião do desmame, o peso médio e o número de leitões foram similares numericamente entre as 6 leitegadas avaliadas durante o período experimental. No entanto, no GT observou-se maior desuniformidade entre os leitões, uma vez que alguns animais ganharam peso mais rápido pelo acesso também ao leite artificial. Apesar de auxiliar na sobrevivência dos leitões, evitando maiores taxas de mortalidade pré-desmame, principalmente nos primeiros dias após o parto, não houve contribuição do equipamento sobre a uniformidade dos lotes. Concluímos que a “mãe de leite eletrônica” possibilitou não só o interesse como também a ingestão de leite artificial pelos leitões, de modo com que mais tetos ficassem disponíveis para outros leitões durante a mamada. Evidenciamos também que tanto leitões refugos como os mais fracos se beneficiaram do aleitamento misto, mantendo seu desenvolvimento de forma adequada até o desmame. Por fim, a equipe indica a utilização do equipamento, no mínimo, durante os 14 primeiros dias de vida dos leitões